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The map label for this gene is puuB [H]

Identifier: 78067967

GI number: 78067967

Start: 3669045

End: 3670520

Strand: Direct

Name: puuB [H]

Synonym: Bcep18194_A6498

Alternate gene names: 78067967

Gene position: 3669045-3670520 (Clockwise)

Preceding gene: 78067966

Following gene: 78067970

Centisome position: 99.32

GC content: 69.24

Gene sequence:

>1476_bases
ATGGTCCCCGCCGCATGCACGTTCGCATGCGGCGGCCGGTGTTTTTCGGGCCGTGCGCGGCACGGCCCCTTTTCGAAGCC
CCCTCTCATGCGACCCTTCTGGATCGAACAAGCGCTGTTCAACGACGGCGATCTCGCCCCCGCGCTGCAAGGCGAAACGC
GGGCCGACGTGTGCATCGTCGGCGGCGGTTTCACCGGGCTCTGGACGGCAATCCAGGCGAAGCAGCAGAACCCCGCGCTC
GACATCGCGATCGTCGAGGCCGACCTGTGCGGCGCCGGCGCGAGCGGTCGCAACGGCGGTTGCCTGCTCACGTGGTCCGC
AAAATTCCTGACGCTGCGGCGGCTGTTCGGCGAAGCCGAGGCGATCCGGCTCGTGAAGGCATCCGAAGCGGCCGTGCAGC
ACATCGCCGATTTCTGCCGCGCGCACGACATCGATGCCGAGCTGCGGCTCGACGGCACGCTGTACACCGCGACGTCACGC
GCGCAGGTCGGCACGCTCGCGCCGGTACTCGACGCACTCGCCGCGTGCGGCATCCGCAGCTACGAGCCGCTGCCGCCCGC
CGAAGTCGCGCGCCGCTCGGGGTCCGCTCGCAATCTCGACGGCGTCTACTCGCCGATCGCCGCGACCGTGCATCCCGGCA
AGCTCGTGCGCGGGCTGCGCCGCGTCGCGCTCGCGATGGGCATCCGGATCTACGAACGCACGCCGATGCTCGACTTCACG
CCCGGGCAACCGGCCGTCGTGCGCACGCCGTCCGGCAGCGTGAGCGCGGGCAAGCTCGTGTTCGCGATCAATGCGTGGAT
GGCGAGCCGCTTCCCGCAGTTCGAGCGCACGATCGCGGTCGTGTCGAGCGACATGGTCATCACCGAGAAATGCCCGGAAC
TGCTCGAGAAAACCGGGCTCGTGGATGGCGTATCCGTGCTCGATTCGCGGATCTTCGTCTACTACTACCGCACCACCGCG
GACGGGCGGCTGATGCTCGGCAAGGGCGGCAACACGTTCTCGTGGCGTAGCCGCATCGCGCCCGTGTTCGACCGGCGCTC
GCCGTACGAGGCGCAGCTCACGCAAAGCCTGCGCGAGTTCTTTCCGTCGCTCGCAGGCGTGCCGGTCACGGCGAGCTGGA
ACGGGCCGTCGGATCGCTCGGTCACCGGCTTCCCGTTCTTCGGCCGCCTCGACGACGCGCCGAACGTGTTCTACGGTTTC
GGCTACTCGGGTAACGGCGTGGGGCCGACCTACATGGGCGGGCAGATCCTGTCGTCGCTCGTGCTCGGTCTCGACAACGC
GTGGACGCGCAGCCCGATCGTGCGCGGCCCGCTTGGGGCGTTCCCGCCGGAGCCGATCCGCTATGTCGGTGCGCACGTCG
TGCGCAATGCGATCCGCCGCAAGGAGCGCGCGGAGGACGAAAGCCGGCAGCCGGCCGTTGTCGACACGTGGCTCGCGAAA
TTCGCGAGCGCGGCCGGGAAGGCAGACAAGGCGTGA

Upstream 100 bases:

>100_bases
GGGCGCCGTCGCCGCGATCGCCGACACGCTGCGCGCGATGGGTGTGCGCCGCGTGTCGGCCGAAGCGGCAGCGTGACGTG
ACGCACCACGACGTGACGTG

Downstream 100 bases:

>100_bases
CGCTGCGTTGGTCTGCAGGACCTGCATGACCTGAAGACGCAATGAAAAAGGGACGCTGCGAAGCGTCCCTTTGCTTTGTG
CCTTGTGCTGTACGCGGCAA

Product: FAD dependent oxidoreductase

Products: NA

Alternate protein names: Gamma-Glu-Put oxidase; Gamma-glutamylputrescine oxidase [H]

Number of amino acids: Translated: 491; Mature: 491

Protein sequence:

>491_residues
MVPAACTFACGGRCFSGRARHGPFSKPPLMRPFWIEQALFNDGDLAPALQGETRADVCIVGGGFTGLWTAIQAKQQNPAL
DIAIVEADLCGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSR
AQVGTLAPVLDALAACGIRSYEPLPPAEVARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFT
PGQPAVVRTPSGSVSAGKLVFAINAWMASRFPQFERTIAVVSSDMVITEKCPELLEKTGLVDGVSVLDSRIFVYYYRTTA
DGRLMLGKGGNTFSWRSRIAPVFDRRSPYEAQLTQSLREFFPSLAGVPVTASWNGPSDRSVTGFPFFGRLDDAPNVFYGF
GYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGAFPPEPIRYVGAHVVRNAIRRKERAEDESRQPAVVDTWLAK
FASAAGKADKA

Sequences:

>Translated_491_residues
MVPAACTFACGGRCFSGRARHGPFSKPPLMRPFWIEQALFNDGDLAPALQGETRADVCIVGGGFTGLWTAIQAKQQNPAL
DIAIVEADLCGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSR
AQVGTLAPVLDALAACGIRSYEPLPPAEVARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFT
PGQPAVVRTPSGSVSAGKLVFAINAWMASRFPQFERTIAVVSSDMVITEKCPELLEKTGLVDGVSVLDSRIFVYYYRTTA
DGRLMLGKGGNTFSWRSRIAPVFDRRSPYEAQLTQSLREFFPSLAGVPVTASWNGPSDRSVTGFPFFGRLDDAPNVFYGF
GYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGAFPPEPIRYVGAHVVRNAIRRKERAEDESRQPAVVDTWLAK
FASAAGKADKA
>Mature_491_residues
MVPAACTFACGGRCFSGRARHGPFSKPPLMRPFWIEQALFNDGDLAPALQGETRADVCIVGGGFTGLWTAIQAKQQNPAL
DIAIVEADLCGAGASGRNGGCLLTWSAKFLTLRRLFGEAEAIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSR
AQVGTLAPVLDALAACGIRSYEPLPPAEVARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFT
PGQPAVVRTPSGSVSAGKLVFAINAWMASRFPQFERTIAVVSSDMVITEKCPELLEKTGLVDGVSVLDSRIFVYYYRTTA
DGRLMLGKGGNTFSWRSRIAPVFDRRSPYEAQLTQSLREFFPSLAGVPVTASWNGPSDRSVTGFPFFGRLDDAPNVFYGF
GYSGNGVGPTYMGGQILSSLVLGLDNAWTRSPIVRGPLGAFPPEPIRYVGAHVVRNAIRRKERAEDESRQPAVVDTWLAK
FASAAGKADKA

Specific function: Involved in the breakdown of putrescine via the oxidation of L-glutamylputrescine [H]

COG id: COG0665

COG function: function code E; Glycine/D-amino acid oxidases (deaminating)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1787559, Length=372, Percent_Identity=29.5698924731183, Blast_Score=153, Evalue=2e-38,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006076 [H]

Pfam domain/function: PF01266 DAO [H]

EC number: 1.-.-.- [C]

Molecular weight: Translated: 52849; Mature: 52849

Theoretical pI: Translated: 9.35; Mature: 9.35

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.8 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.5 %Cys+Met (Translated Protein)
1.8 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPAACTFACGGRCFSGRARHGPFSKPPLMRPFWIEQALFNDGDLAPALQGETRADVCIV
CCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
GGGFTGLWTAIQAKQQNPALDIAIVEADLCGAGASGRNGGCLLTWSAKFLTLRRLFGEAE
CCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHH
AIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSRAQVGTLAPVLDALAACGIRS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCC
YEPLPPAEVARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFT
CCCCCHHHHHHCCCCCCCCCHHHHCHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEC
PGQPAVVRTPSGSVSAGKLVFAINAWMASRFPQFERTIAVVSSDMVITEKCPELLEKTGL
CCCCEEEECCCCCCCCCEEEEEEEHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCC
VDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPYEAQLTQSLREF
CHHHHHHCCEEEEEEEEECCCCEEEEECCCCCCHHHHHCCHHHCCCCCHHHHHHHHHHHH
FPSLAGVPVTASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSL
HHHHCCCCEEECCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
VLGLDNAWTRSPIVRGPLGAFPPEPIRYVGAHVVRNAIRRKERAEDESRQPAVVDTWLAK
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
FASAAGKADKA
HHHHCCCCCCC
>Mature Secondary Structure
MVPAACTFACGGRCFSGRARHGPFSKPPLMRPFWIEQALFNDGDLAPALQGETRADVCIV
CCCCCEEHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCCCCCCCCCCEEEE
GGGFTGLWTAIQAKQQNPALDIAIVEADLCGAGASGRNGGCLLTWSAKFLTLRRLFGEAE
CCCHHHHHHHHHHHCCCCCEEEEEEEEECCCCCCCCCCCCEEEEEHHHHHHHHHHHCCHH
AIRLVKASEAAVQHIADFCRAHDIDAELRLDGTLYTATSRAQVGTLAPVLDALAACGIRS
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEECCEEEEECCCCCHHHHHHHHHHHHHCCCCC
YEPLPPAEVARRSGSARNLDGVYSPIAATVHPGKLVRGLRRVALAMGIRIYERTPMLDFT
CCCCCHHHHHHCCCCCCCCCHHHHCHHHCCCCHHHHHHHHHHHHHHCCEEEECCCCEEEC
PGQPAVVRTPSGSVSAGKLVFAINAWMASRFPQFERTIAVVSSDMVITEKCPELLEKTGL
CCCCEEEECCCCCCCCCEEEEEEEHHHHHCCCHHHHHHHHHCCCEEEHHHHHHHHHHCCC
VDGVSVLDSRIFVYYYRTTADGRLMLGKGGNTFSWRSRIAPVFDRRSPYEAQLTQSLREF
CHHHHHHCCEEEEEEEEECCCCEEEEECCCCCCHHHHHCCHHHCCCCCHHHHHHHHHHHH
FPSLAGVPVTASWNGPSDRSVTGFPFFGRLDDAPNVFYGFGYSGNGVGPTYMGGQILSSL
HHHHCCCCEEECCCCCCCCCEECCCCCCCCCCCCCEEEECCCCCCCCCCCHHHHHHHHHH
VLGLDNAWTRSPIVRGPLGAFPPEPIRYVGAHVVRNAIRRKERAEDESRQPAVVDTWLAK
HHCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCHHCCCCHHHHHHHHH
FASAAGKADKA
HHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503; 1840553; 7984428 [H]