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The map label for this gene is exoA [H]

Identifier: 78067925

GI number: 78067925

Start: 3622008

End: 3622781

Strand: Reverse

Name: exoA [H]

Synonym: Bcep18194_A6456

Alternate gene names: 78067925

Gene position: 3622781-3622008 (Counterclockwise)

Preceding gene: 78067926

Following gene: 78067923

Centisome position: 98.07

GC content: 62.02

Gene sequence:

>774_bases
ATGATGCGAGTGATTACCGCCAACCTGAACGGCATCCGCTCCGCCGCGAAGAAGGGCTTCTTCGAGTGGCTCGGCGAACA
GAACGCCGATTGCGTGTGCGTGCAGGAAATCAAGGTCTCGGCCGACGACCTGCCGGCCGAATTCGTCGAGCCGCACGGCT
TCAAGAGCTATTTCCACCACGCCGAGAAGAAGGGCTACAGCGGCGCGGGCGTGTACAGCCGCCGCGAGCCCGATGACGTG
ATCATCGGCTTCGGCAGCAGCGAATTCGATGCCGAAGGGCGCTACGTCGAGGCGCGCTACGGCAAGCTGTCGGTCGTGTC
GGTGTACGTGCCGTCCGGCTCGAGCGGCGAAGAGCGCCAGCAGGCGAAGTACCGCTTCATGGACGAGTTCATGCCGCACC
TCGCCGCGCTGAAGAAGAAGCGCGAGGTGATCCTGTGCGGCGACGTGAACATCGTCCACAAGGAAATCGACATCAAGAAC
TGGAAGAGCAACCAGAAGAACTCGGGCTGCCTGCCGGAAGAGCGCGAATGGCTCACGAAGCTGTTCGACGATGTCGGCTA
TGTCGACGTGTTCCGCACGCTCGACCCGCGCGCCGAGCAGTACACGTGGTGGAGCAACCGCGGCCAGGCGTATGCGAAGA
ACGTCGGGTGGCGGATCGATTACCAGATCGCGACGCCGGGTGTGGCCGGCACCGCGAAAAGCACGTCGATCTTCAAGGAC
ATCAAGTTCAGCGATCATGCGCCGCTGACGATCGACTACGACTACAAGAAGTGA

Upstream 100 bases:

>100_bases
ACATGAAGCTGGAATTCCCGCCGCCGAAGCCCGAGCTCGAAGGCGTGAAGATCCGCTAAGCTGCACCCCTGAAAAAACAT
CGAACTGAACGGAATCCGAC

Downstream 100 bases:

>100_bases
CGTCCCGCGCTGCGCCGCGCGCGTAGCAGCCGGATCGTCCGGCCGCCGGATGCAAAAACGGGCCGCATGTCGAACATGCG
GCCCGTTTCTTTTTGGGGCG

Product: exodeoxyribonuclease III xth

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 257; Mature: 257

Protein sequence:

>257_residues
MMRVITANLNGIRSAAKKGFFEWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDAEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMDEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDPRAEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYKK

Sequences:

>Translated_257_residues
MMRVITANLNGIRSAAKKGFFEWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDAEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMDEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDPRAEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYKK
>Mature_257_residues
MMRVITANLNGIRSAAKKGFFEWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHHAEKKGYSGAGVYSRREPDDV
IIGFGSSEFDAEGRYVEARYGKLSVVSVYVPSGSSGEERQQAKYRFMDEFMPHLAALKKKREVILCGDVNIVHKEIDIKN
WKSNQKNSGCLPEEREWLTKLFDDVGYVDVFRTLDPRAEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
IKFSDHAPLTIDYDYKK

Specific function: Major Apurinic-Apyrimidinic Endonuclease Of E.Coli. It Removes The Damaged DNA At Cytosines And Guanines By Cleaving On The 3' Side Of The Ap Site By A Beta-Elimination Reaction. It Exhibits 3'-5'-Exonuclease, 3'-Phosphomonoesterase, 3'-Repair Diesterase

COG id: COG0708

COG function: function code L; Exonuclease III

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the DNA repair enzymes AP/ExoA family [H]

Homologues:

Organism=Homo sapiens, GI18375505, Length=253, Percent_Identity=35.9683794466403, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375503, Length=253, Percent_Identity=35.9683794466403, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375501, Length=253, Percent_Identity=35.9683794466403, Blast_Score=176, Evalue=2e-44,
Organism=Homo sapiens, GI18375507, Length=311, Percent_Identity=27.0096463022508, Blast_Score=91, Evalue=8e-19,
Organism=Escherichia coli, GI1788046, Length=267, Percent_Identity=31.4606741573034, Blast_Score=111, Evalue=4e-26,
Organism=Caenorhabditis elegans, GI71989536, Length=259, Percent_Identity=30.8880308880309, Blast_Score=137, Evalue=5e-33,
Organism=Drosophila melanogaster, GI221330655, Length=251, Percent_Identity=34.2629482071713, Blast_Score=150, Evalue=8e-37,
Organism=Drosophila melanogaster, GI17136678, Length=251, Percent_Identity=34.2629482071713, Blast_Score=149, Evalue=1e-36,

Paralogues:

None

Copy number: 900 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000097
- InterPro:   IPR020847
- InterPro:   IPR020848
- InterPro:   IPR005135
- InterPro:   IPR004808 [H]

Pfam domain/function: PF03372 Exo_endo_phos [H]

EC number: =3.1.11.2 [H]

Molecular weight: Translated: 29251; Mature: 29251

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MMRVITANLNGIRSAAKKGFFEWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHH
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCCCCHHHHHHH
AEKKGYSGAGVYSRREPDDVIIGFGSSEFDAEGRYVEARYGKLSVVSVYVPSGSSGEERQ
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMDEFMPHLAALKKKREVILCGDVNIVHKEIDIKNWKSNQKNSGCLPEEREWLTK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDDVGYVDVFRTLDPRAEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
HHHCCCHHHHHHHCCCCCHHEEEECCCCCEEHHCCCEEEEEEEECCCCCCCCCCHHHHHH
IKFSDHAPLTIDYDYKK
CCCCCCCCEEEECCCCC
>Mature Secondary Structure
MMRVITANLNGIRSAAKKGFFEWLGEQNADCVCVQEIKVSADDLPAEFVEPHGFKSYFHH
CEEEEECCCHHHHHHHHHHHHHHHCCCCCCEEEEEEEECCCCCCCHHHCCCCCHHHHHHH
AEKKGYSGAGVYSRREPDDVIIGFGSSEFDAEGRYVEARYGKLSVVSVYVPSGSSGEERQ
HHHCCCCCCCCCCCCCCCCEEEECCCCCCCCCCCEEEEECCEEEEEEEEECCCCCCHHHH
QAKYRFMDEFMPHLAALKKKREVILCGDVNIVHKEIDIKNWKSNQKNSGCLPEEREWLTK
HHHHHHHHHHHHHHHHHHCCCCEEEECCCEEEEEEECCCCCCCCCCCCCCCCHHHHHHHH
LFDDVGYVDVFRTLDPRAEQYTWWSNRGQAYAKNVGWRIDYQIATPGVAGTAKSTSIFKD
HHHCCCHHHHHHHCCCCCHHEEEECCCCCEEHHCCCEEEEEEEECCCCCCCCCCHHHHHH
IKFSDHAPLTIDYDYKK
CCCCCCCCEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 7584024; 9384377 [H]