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The map label for this gene is slt [H]

Identifier: 78067850

GI number: 78067850

Start: 3548157

End: 3549980

Strand: Reverse

Name: slt [H]

Synonym: Bcep18194_A6381

Alternate gene names: 78067850

Gene position: 3549980-3548157 (Counterclockwise)

Preceding gene: 78067852

Following gene: 78067849

Centisome position: 96.1

GC content: 67.21

Gene sequence:

>1824_bases
GTGCAGCTTCGTGAAGCCGCGCGTCGCAACGACGCTGCGAAAGCCGCGCAGCTCGCGTCGATGATCCCGAACTATCCGGT
ACCGTCCTACGTCGAGTATTTCCAGATCAAGCCGCAGTTGTTCGATTCGACGGGCCGCGCGCGCGTGGACGCGCCGGACG
CGCCCGTGCAGTCGTTCCTGCAGCGCTACGACGGCCAGGCGATCGCCGACCGCCTGCGCAACGACTACCTGCTCGTGCTC
GGCGCGCGCCACGACTGGCGCAACTTCGACGACCAGTACAAGCGCTTCGTGCTCGACGACGACACGCAGGTCAAGTGCTA
TGCGCTCGAATCGCGCGCGGCGCGCGGCGAGAACGTCGCGGATGCGGCACGTGCGCTGCTCGTCGAGCCGAAGAATTACG
GCGACGCGTGCGTCGACCTGATCACCGCGCTGACGGTCAACCAGCAGTTCACGAGCGACGACGTGTGGCAGCAGGCGCGT
CTCGCGTACGAGCAGAACTACACGACGCTCGGCGGCAAGATCGTCGATGCGCTCGGCCCGCGCCCGGTCGGCTTCGACCA
GGCGACGAGTGCGCCGCCGCTGTACCTCGCGCGCGGCGTCGGCCCCGACGCGACCTCGCACCAGCTCGCGCTGATCGCGC
TCAGCCGCATGGCGCGCAACGATCCGGACGCGGCAGCCGGGATGCTGACGTCGGTCGCCGCCTCGCTGACCAAGCAGGAA
CAGGCGATCGCGTGGGGCGCGATCGGCTACCAGGGCGCGATCAAGCGCTCGCCGCTCGCATCGGTCTGGTACGCGAAATC
CACGAATGCGCCGCTGTCGAACCCGGGCTACGAATGGCGCACGCGCGCCGCGCTGCTCGCCGGCAACTGGCCGATGGTGC
GCTGGTCGATCGAGCAGATGCCGCCGTCGCTGCGCGACGATCCGGCCTGGATCTACTGGCATGCGCGCGCGCTCAAGCAG
AGCGGCGACACGCTGCAGGCGAACCAGGAATTCGAGCAGGTCGCGGGCCAGTTCAACTTCTACGGGCAGCTCGCCGGCGA
AGAGCTCGGCCAGCGCACGACGATCCCGCCGCGCACGAAGGTGAGCGACGCCGAGATCGACGCGATGAGCAAGATTCCGG
GCTTCGCGCTCGCGCAGCGTTTCTACGCGCTGAACCTGCGCCTCGAAGGCAACCGCGAATGGAACTGGCCGCTGCGCGGG
ATGACCGATCGCCAGCTGCTGGCCGCAGCCGAATACGGCAAGCGCGTCGAGCTGCTCGACCGCACGGTCAACACGGCCGA
CCGCACGAAGGCCGAGCACGACTTCACGCTGCGCTACCCGTCGCCGTACCGCAATATCGTCGAGCGCTATGCGCAATCGA
CGGGCCTCGACATCGAGTGGGCGTACGGGCTGATCCGCCAGGAATCGCGCTTCATCATCAGCGCGCGTTCGTCGGTGGGC
GCGGGCGGCCTGATGCAGCTGATGCCGGCCACCGCGCAGATGGTCGCGAAGAAGCTCGGCATGGGCACGATCTCGCGTGC
GCAGATGCACGACATCGATACCAATATCCAGCTCGGCACCTGGTATCTGGCGGACATCTACAACAACTTCGACAGCTCGC
CGGTGCTCGCCACGGCCGGCTACAACGCGGGCCCGGGCCGGCCGCGCCAGTGGCGCCAGGTGTTGACGCAGCCGGTCGAA
GGCGCGATCTTCGCAGAAACGATTCCGTTCAACGAGACGCGCGACTACGTGAAGAACGTGCTGTCGAACACCGTCTATTA
CGCGGCGCTCTTCGAGGGCAAACCGCAGTCGTTGAAGAAACGTCTAGGCATGATCTCGCCCTGA

Upstream 100 bases:

>100_bases
CGCGGTCGCGCTGACGCTTTCGGCCACCGCGCTCGTCGCGGGCACGGCTGCGTGCGCCGCACCGAACGACGATACGGTCT
CCGGGGACGACCAGACCTTC

Downstream 100 bases:

>100_bases
TACGCAATCTTCACACTGCCGCGCGAGACGCGGCAGTGTTTAAGGGGCTGTTCACGCTAATAACGGGCTTGCGCTGGCCG
CCAGAAGGGCCGAGCGCAAG

Product: lytic transglycosylase, catalytic

Products: 1,6-Anhydrobond [C]

Alternate protein names: Exomuramidase; Peptidoglycan lytic exotransglycosylase; Slt70 [H]

Number of amino acids: Translated: 607; Mature: 607

Protein sequence:

>607_residues
MQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVL
GARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARGENVADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQAR
LAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLARGVGPDATSHQLALIALSRMARNDPDAAAGMLTSVAASLTKQE
QAIAWGAIGYQGAIKRSPLASVWYAKSTNAPLSNPGYEWRTRAALLAGNWPMVRWSIEQMPPSLRDDPAWIYWHARALKQ
SGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTKVSDAEIDAMSKIPGFALAQRFYALNLRLEGNREWNWPLRG
MTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTLRYPSPYRNIVERYAQSTGLDIEWAYGLIRQESRFIISARSSVG
AGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVE
GAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKKRLGMISP

Sequences:

>Translated_607_residues
MQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVL
GARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARGENVADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQAR
LAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLARGVGPDATSHQLALIALSRMARNDPDAAAGMLTSVAASLTKQE
QAIAWGAIGYQGAIKRSPLASVWYAKSTNAPLSNPGYEWRTRAALLAGNWPMVRWSIEQMPPSLRDDPAWIYWHARALKQ
SGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTKVSDAEIDAMSKIPGFALAQRFYALNLRLEGNREWNWPLRG
MTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTLRYPSPYRNIVERYAQSTGLDIEWAYGLIRQESRFIISARSSVG
AGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVE
GAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKKRLGMISP
>Mature_607_residues
MQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFLQRYDGQAIADRLRNDYLLVL
GARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARGENVADAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQAR
LAYEQNYTTLGGKIVDALGPRPVGFDQATSAPPLYLARGVGPDATSHQLALIALSRMARNDPDAAAGMLTSVAASLTKQE
QAIAWGAIGYQGAIKRSPLASVWYAKSTNAPLSNPGYEWRTRAALLAGNWPMVRWSIEQMPPSLRDDPAWIYWHARALKQ
SGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTKVSDAEIDAMSKIPGFALAQRFYALNLRLEGNREWNWPLRG
MTDRQLLAAAEYGKRVELLDRTVNTADRTKAEHDFTLRYPSPYRNIVERYAQSTGLDIEWAYGLIRQESRFIISARSSVG
AGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAGYNAGPGRPRQWRQVLTQPVE
GAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKKRLGMISP

Specific function: Murein-degrading enzyme. Catalyzes the cleavage of the glycosidic bonds between N-acetylmuramic acid and N- acetylglucosamine residues in peptidoglycan. May play a role in recycling of muropeptides during cell elongation and/or cell division [H]

COG id: COG0741

COG function: function code M; Soluble lytic murein transglycosylase and related regulatory proteins (some contain LysM/invasin domains)

Gene ontology:

Cell location: Periplasm. Note=Tightly associated with the murein sacculus (By similarity) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the transglycosylase slt family [H]

Homologues:

Organism=Escherichia coli, GI87082441, Length=612, Percent_Identity=25.6535947712418, Blast_Score=178, Evalue=9e-46,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016026
- InterPro:   IPR008258
- InterPro:   IPR012289
- InterPro:   IPR008939
- InterPro:   IPR000189 [H]

Pfam domain/function: PF01464 SLT [H]

EC number: 3.2.1.- [C]

Molecular weight: Translated: 67744; Mature: 67744

Theoretical pI: Translated: 8.70; Mature: 8.70

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFL
CCCHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCCEECCCCCHHHHHHH
QRYDGQAIADRLRNDYLLVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARGENVA
HHHCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHEEEECCCCCEEEEEECCCCCCCCCHH
DAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGP
HHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHCCC
RPVGFDQATSAPPLYLARGVGPDATSHQLALIALSRMARNDPDAAAGMLTSVAASLTKQE
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QAIAWGAIGYQGAIKRSPLASVWYAKSTNAPLSNPGYEWRTRAALLAGNWPMVRWSIEQM
HHHEEECCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCHHHCEEEEECCCCEEEEEHHHC
PPSLRDDPAWIYWHARALKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTK
CCCCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
VSDAEIDAMSKIPGFALAQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLD
CCCCHHHHHHHCCHHHHHHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
RTVNTADRTKAEHDFTLRYPSPYRNIVERYAQSTGLDIEWAYGLIRQESRFIISARSSVG
HHHCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCC
AGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAG
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCEEHHHHHCCCCCCCEEEECC
YNAGPGRPRQWRQVLTQPVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKK
CCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHH
RLGMISP
HCCCCCC
>Mature Secondary Structure
MQLREAARRNDAAKAAQLASMIPNYPVPSYVEYFQIKPQLFDSTGRARVDAPDAPVQSFL
CCCHHHHHHCHHHHHHHHHHHCCCCCCCHHHHHHCCCHHHHCCCCCEECCCCCHHHHHHH
QRYDGQAIADRLRNDYLLVLGARHDWRNFDDQYKRFVLDDDTQVKCYALESRAARGENVA
HHHCCHHHHHHHCCCEEEEEECCCCCCCCCHHHHEEEECCCCCEEEEEECCCCCCCCCHH
DAARALLVEPKNYGDACVDLITALTVNQQFTSDDVWQQARLAYEQNYTTLGGKIVDALGP
HHHHHHEECCCCCCHHHHHHHHHHHCCCCCCCHHHHHHHHHHHHCCCHHCCCHHHHHCCC
RPVGFDQATSAPPLYLARGVGPDATSHQLALIALSRMARNDPDAAAGMLTSVAASLTKQE
CCCCCCCCCCCCCEEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHH
QAIAWGAIGYQGAIKRSPLASVWYAKSTNAPLSNPGYEWRTRAALLAGNWPMVRWSIEQM
HHHEEECCCCCCCCCCCCCHHEEEECCCCCCCCCCCCCHHHCEEEEECCCCEEEEEHHHC
PPSLRDDPAWIYWHARALKQSGDTLQANQEFEQVAGQFNFYGQLAGEELGQRTTIPPRTK
CCCCCCCCCEEEEEHHHHHCCCCCCHHHHHHHHHHHCCCHHHHHHHHHHCCCCCCCCCCC
VSDAEIDAMSKIPGFALAQRFYALNLRLEGNREWNWPLRGMTDRQLLAAAEYGKRVELLD
CCCCHHHHHHHCCHHHHHHHEEEEEEEEECCCCCCCCCCCCCHHHHHHHHHHCCHHHHHH
RTVNTADRTKAEHDFTLRYPSPYRNIVERYAQSTGLDIEWAYGLIRQESRFIISARSSVG
HHHCHHHHCCCCCCEEEECCCHHHHHHHHHHHHCCCCCHHHHHHHHCCCEEEEEECCCCC
AGGLMQLMPATAQMVAKKLGMGTISRAQMHDIDTNIQLGTWYLADIYNNFDSSPVLATAG
CCHHHHHHHHHHHHHHHHHCCCCCCHHHHHCCCCCEEECCEEHHHHHCCCCCCCEEEECC
YNAGPGRPRQWRQVLTQPVEGAIFAETIPFNETRDYVKNVLSNTVYYAALFEGKPQSLKK
CCCCCCCCHHHHHHHHCCCCCCEEEECCCCCHHHHHHHHHHHCCEEEEEEECCCHHHHHH
RLGMISP
HCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Muramic Acid Residue (N-Acetylmuramic Acid And N-Acetylglucosamine Residues) [C]

Specific reaction: Cleavage Of The Beta-1,4-Glycosidic Bond Between N-Acetylmuramic Acid And N-Acetylglucosamine Residues, Thereby Conserving The Energy In A Newly Synthesized 1,6-Anhydrobond In The Muramic Acid Residue. [C]

General reaction: Cleavage Of The Beta-1,4-Glycosidic Bond [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]