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The map label for this gene is potI [H]

Identifier: 78067817

GI number: 78067817

Start: 3512271

End: 3513110

Strand: Direct

Name: potI [H]

Synonym: Bcep18194_A6348

Alternate gene names: 78067817

Gene position: 3512271-3513110 (Clockwise)

Preceding gene: 78067816

Following gene: 78067818

Centisome position: 95.08

GC content: 64.17

Gene sequence:

>840_bases
ATGATCAAGCCGAGCAAACCGCTGTCGACGGGCGTCCTCGCCTTCGGCTTCCTGTTCCTGTACATCCCGATCATCAGCCT
GGTCGTGTACTCGTTCAACGAGTCGAAGCTGGTGACGGTGTGGTCGGGCTTCTCGCTGAAGTGGTACTCGGCGCTGCTGG
ACGACGATGAGCTGCTCACTGCCGCATGGCTGTCGCTGAAGATCGGTCTCTTGACCGCGACCGCGTCGGTCGTGATCGGC
ACGTGGGCCGGCTTCGTGCTCGCACGCTTCGGCCGCTTCAAGGGCTTCACGCTGTACACGGGGATGATCAACGCGCCGCT
GGTGATTCCGGAAGTGATCCAGGGCATCTCGCTGCTGTTGCTGTTCGTTGCGCTGGAGCAGATGTTCGGCTGGCCGAAGG
GACGCGGGATGGTGACGATCTGGATCGGTCACGTGATGCTGTGCGTGTCGTACGTGGCGATCATCGTGCAGTCGCGCGTG
AAGGAGATGAACAAGTCGCTGGAAGAGGCGGCGCTGGATCTGGGGGCGACGCCGCTGAAGGTGTTCTTCGTGGTGACGCT
GCCGCTGATCTCGCAGGCGCTGCTGTCGGGGTGGCTGCTGTCGTTCACGCTGTCGATCGACGACCTGGTGCTGTCGGCGT
TCCTGTCCGGGCCGGGCTCGACGACGCTGCCGCTGGTGGTGTTCTCGCGCGTGCGGCTGGGGCTGAACCCGGAGATGAAC
GCACTGGCGACGCTGTTCATCACGGCGGTGACGATCGGCGTGATCGTCGTGAACCGGATGATGATCGCGCGCGAGCGGCG
CCGCATCGCCGACATGAAGGCGGCGTTCGCGGTGGCGTGA

Upstream 100 bases:

>100_bases
GGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGATGCTGCTGCTGGTGCCGATGGCGCTGTTCCAGTACTACCAGGTC
AAGGAACTGGAGGACGCGAA

Downstream 100 bases:

>100_bases
CGCCCGGCCTCCTGCTTCAACGATAACAAGCACTGCAGACAAGCAATACGCGCTGCCCCAGGGGGGGCGCGCGATTCGAT
TCCGATTTGACAGGCGCACG

Product: ABC spermidine/putrescine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 279; Mature: 279

Protein sequence:

>279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYSALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRIADMKAAFAVA

Sequences:

>Translated_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYSALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRIADMKAAFAVA
>Mature_279_residues
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYSALLDDDELLTAAWLSLKIGLLTATASVVIG
TWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLLLFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRV
KEMNKSLEEAALDLGATPLKVFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
ALATLFITAVTIGVIVVNRMMIARERRRIADMKAAFAVA

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1177

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component II

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787081, Length=270, Percent_Identity=56.6666666666667, Blast_Score=288, Evalue=3e-79,
Organism=Escherichia coli, GI1787368, Length=241, Percent_Identity=38.5892116182573, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI1787714, Length=245, Percent_Identity=32.2448979591837, Blast_Score=87, Evalue=1e-18,
Organism=Escherichia coli, GI1788764, Length=219, Percent_Identity=30.1369863013699, Blast_Score=62, Evalue=3e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 30483; Mature: 30483

Theoretical pI: Translated: 10.05; Mature: 10.05

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.6 %Met     (Translated Protein)
3.9 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.6 %Met     (Mature Protein)
3.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYSALLDDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCHHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNRMMIARERRRIADMKAAFAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MIKPSKPLSTGVLAFGFLFLYIPIISLVVYSFNESKLVTVWSGFSLKWYSALLDDDELLT
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCCCHHHHHHHCCCHHHHH
AAWLSLKIGLLTATASVVIGTWAGFVLARFGRFKGFTLYTGMINAPLVIPEVIQGISLLL
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCHHHHHHHHHHHHH
LFVALEQMFGWPKGRGMVTIWIGHVMLCVSYVAIIVQSRVKEMNKSLEEAALDLGATPLK
HHHHHHHHHCCCCCCCEEEHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHH
VFFVVTLPLISQALLSGWLLSFTLSIDDLVLSAFLSGPGSTTLPLVVFSRVRLGLNPEMN
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHCCCCCCHH
ALATLFITAVTIGVIVVNRMMIARERRRIADMKAAFAVA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 12471157 [H]