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The map label for this gene is potH [H]

Identifier: 78067816

GI number: 78067816

Start: 3511288

End: 3512274

Strand: Direct

Name: potH [H]

Synonym: Bcep18194_A6347

Alternate gene names: 78067816

Gene position: 3511288-3512274 (Clockwise)

Preceding gene: 78067815

Following gene: 78067817

Centisome position: 95.05

GC content: 64.13

Gene sequence:

>987_bases
ATGAGAACCTCCGCCTCCAATCCGTCCGCGCCGGTGCCGACCGGCGCCGCGAGCGCCGGCTCCGGTCGCGCCCGCCCGGG
CCGCTTCTCGATGCTGCAGCGCTTCCTGCCGTCGGGCCGCAGCGTCGCGATCGGCGTGCCGTTCGTGTGGCTCGCCGTGT
TCTTCGCGCTGCCGTTCGTGCTGGTGCTGAAGATCAGCTTCGCCGACCAGGTGATGGGCATCCCGCCGTACACGTCACTC
GTCGAGTTCAAGGACGGCGTCGTGCACTTCGCGCTGCAGCTGTCGCACTACGCGTTCCTGCTGCAGGACGACCTGTACAT
CGCGACCTACCTCAGCTCGCTGAAGATGGCCGCCGTATCGACGGTGCTGTGTCTGCTGATCGGCTATCCGATGGCGTACT
ACATCGCGCGCTCGGAACCGAACCGCCGCAACGTGCTGATGATGGCCGTGATGCTGCCGTTCTGGACGTCGTTCCTGATC
CGCGTGTACGCCTGGATCGGCATCCTGAAGGATGACGGCCTGCTGAACCACACGCTGATCGCGCTCGGCATCATCCACAC
GCCGCTGCGCCTTTACCACAGCGATGCAGGTGTCTACATCGGGATGGTCTATTCGTACCTGCCGTTCATGGTGATGCCGC
TGTACGCGCACCTCGTGAAGATGGACCTCACGCTGCTCGAAGCCGCGTACGACCTCGGCGCGAAGCCGTGGGTCGCGTTC
ACGCGGATCACGCTGCCGCTGTCGAAGAACGGGATCATCGCCGGCAGCCTGCTGGTGTTCATCCCGGCAGTCGGCGAGTA
CGTGATTCCGGAACTGCTCGGCGGCGCCGACACGCTGATGATCGGCCGTGTGATGTGGGATGAATTCTTCAACAACATGG
ACTGGCCGATGGCGTCCGCGGTGACGGTCGCGATGGTGATGCTGCTGCTGGTGCCGATGGCGCTGTTCCAGTACTACCAG
GTCAAGGAACTGGAGGACGCGAAATGA

Upstream 100 bases:

>100_bases
TCGCACCTGATGACCGACAACGCACCGGCGTGGAACGACGACGTGTTCGTGTCGTGGTCGCCGGCCAGCGGCGTCGTGCT
GACGCAGTGAGGACACCACG

Downstream 100 bases:

>100_bases
TCAAGCCGAGCAAACCGCTGTCGACGGGCGTCCTCGCCTTCGGCTTCCTGTTCCTGTACATCCCGATCATCAGCCTGGTC
GTGTACTCGTTCAACGAGTC

Product: ABC spermidine/putrescine transporter, inner membrane subunit

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MRTSASNPSAPVPTGAASAGSGRARPGRFSMLQRFLPSGRSVAIGVPFVWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVMLLLVPMALFQYYQ
VKELEDAK

Sequences:

>Translated_328_residues
MRTSASNPSAPVPTGAASAGSGRARPGRFSMLQRFLPSGRSVAIGVPFVWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVMLLLVPMALFQYYQ
VKELEDAK
>Mature_328_residues
MRTSASNPSAPVPTGAASAGSGRARPGRFSMLQRFLPSGRSVAIGVPFVWLAVFFALPFVLVLKISFADQVMGIPPYTSL
VEFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMAAVSTVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLI
RVYAWIGILKDDGLLNHTLIALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASAVTVAMVMLLLVPMALFQYYQ
VKELEDAK

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine [H]

COG id: COG1176

COG function: function code E; ABC-type spermidine/putrescine transport system, permease component I

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 ABC transmembrane type-1 domain [H]

Homologues:

Organism=Escherichia coli, GI1787080, Length=303, Percent_Identity=53.4653465346535, Blast_Score=320, Evalue=9e-89,
Organism=Escherichia coli, GI1787369, Length=279, Percent_Identity=39.0681003584229, Blast_Score=189, Evalue=2e-49,
Organism=Escherichia coli, GI1787713, Length=221, Percent_Identity=32.1266968325792, Blast_Score=111, Evalue=7e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000515 [H]

Pfam domain/function: PF00528 BPD_transp_1 [H]

EC number: NA

Molecular weight: Translated: 36385; Mature: 36385

Theoretical pI: Translated: 8.34; Mature: 8.34

Prosite motif: PS50928 ABC_TM1

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
5.8 %Met     (Translated Protein)
6.1 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
5.8 %Met     (Mature Protein)
6.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTSASNPSAPVPTGAASAGSGRARPGRFSMLQRFLPSGRSVAIGVPFVWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVEFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHTLI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVMLLLVPMALFQYYQVKELEDAK
HHHHHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MRTSASNPSAPVPTGAASAGSGRARPGRFSMLQRFLPSGRSVAIGVPFVWLAVFFALPFV
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCEEEECCHHHHHHHHHHHHHH
LVLKISFADQVMGIPPYTSLVEFKDGVVHFALQLSHYAFLLQDDLYIATYLSSLKMAAVS
HHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHH
TVLCLLIGYPMAYYIARSEPNRRNVLMMAVMLPFWTSFLIRVYAWIGILKDDGLLNHTLI
HHHHHHHHHHHHHEEECCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHH
ALGIIHTPLRLYHSDAGVYIGMVYSYLPFMVMPLYAHLVKMDLTLLEAAYDLGAKPWVAF
HHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCEEE
TRITLPLSKNGIIAGSLLVFIPAVGEYVIPELLGGADTLMIGRVMWDEFFNNMDWPMASA
EEEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHCCCCCCHHHH
VTVAMVMLLLVPMALFQYYQVKELEDAK
HHHHHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503 [H]