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The map label for this gene is potF [H]

Identifier: 78067814

GI number: 78067814

Start: 3508816

End: 3509934

Strand: Direct

Name: potF [H]

Synonym: Bcep18194_A6345

Alternate gene names: 78067814

Gene position: 3508816-3509934 (Clockwise)

Preceding gene: 78067813

Following gene: 78067815

Centisome position: 94.98

GC content: 63.18

Gene sequence:

>1119_bases
ATGCGTGCCTGCTTTCTTCGCCAAGCCTGTTCCGTTGCCGCCCTTGCCGCCGCGGCCGCGTTCACGTCGGTCGCCAGCCC
GACGGCACACGCGGCCGACGAGCTGAATGTCTACAACTGGTCCGACTACATCGCACCGGACACGATCCCGAACTTCCAGA
AGCAGACGGGCATCCACGTCAAGTACGACAACTACGACAGCGACGACACGCTTCAGGCGAAGCTGCTGGCCGGCAGTTCG
GGCTACGACATCGTCGTGCCGACGTCGAACTACATGGCCAAGCAAATCCAGGCCGGCGTGTACCAGAAGCTCGACAAGTC
GAAGCTGCCGAACCTCGCGAACCTCGACCCGCTGCTGATGAAGATGATCGCGGATGCCGATCCGGGCAACCAGTACGGCG
TGCCCTGGGCGTACGGCACGGACGGCATCGGCTACAACGCGCAGGCAGTGAAGAAGGCACTCGGCGACAAGGCGCCGGTC
GACAGCTGGGCACTCGTGTTCGACCCGGCCAACATGGAAAAGCTGAAGGGCTGCGGCGTATCGTTCCTCGACCAGGCCGT
CGACGTGTTCGCCGCCACGCTGCAATACATGGGCAAGAACCCGAACAGCACGAATCCCGGCGACTACCAGGCTGCGTTCG
AAGTCCTGAAGAAAGTCCGCCCGTACATCACCCAGTTCAACTCGTCCGGCTACATCAACGACCTCGCGAACAACGACGTG
TGCGTCGCGCTCGGCTGGTCGGGCGACGTCGGCATCGCGCATCGCCGCTCGGCCGAAGCGAAGCGTTCGTACGACATCAA
GTTCTCGAACCCGAAGGAAGGCGGCCTGCTGTGGTTCGACGTGATGGTGATCCCGAAGGATGCACCGCACCCCGAGGCCG
CCCTGAAGTGGATCAACTACGTGTCCGATCCGAAGGTCAACGCGGCGATCACCAACACGGTGTTCTACCCGACCGCGAAC
AAGGCCGCACACCAGTTCGTCACGCCGGCCGTCGCGCAAGACCCGACCGTCTACCCGCCGGAAGACGTGCTGAAGAAGAT
GGTGCTGATGAAGCCGATGCCGGCCGACATCCTGCGCCTCCAGAACCGCCTGTGGGCCCAATTGAAGACCGGCCACTGA

Upstream 100 bases:

>100_bases
GCAGTCGATGCAACCGCCCAGCAATTGGGTATTTCGTAACGGTTTTGCCTATAGGCGTGCGGCGGGCGCCGCACGCCGCC
ATAACCAGGGGAATTCCACC

Downstream 100 bases:

>100_bases
TCGCCCAGCATTCCGGCGGTGCAGGCAACGCTTCACGCCTGCCCGCCTGAGATCCGCGGACCTCCGTCCGCCCGCGCGCG
CCGCGCGCACTGTTCCATCC

Product: ABC spermidine/putrescine transporter, periplasmic ligand binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 372; Mature: 372

Protein sequence:

>372_residues
MRACFLRQACSVAALAAAAAFTSVASPTAHAADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSS
GYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPLLMKMIADADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPV
DSWALVFDPANMEKLKGCGVSFLDQAVDVFAATLQYMGKNPNSTNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
CVALGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPEAALKWINYVSDPKVNAAITNTVFYPTAN
KAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLQNRLWAQLKTGH

Sequences:

>Translated_372_residues
MRACFLRQACSVAALAAAAAFTSVASPTAHAADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSS
GYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPLLMKMIADADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPV
DSWALVFDPANMEKLKGCGVSFLDQAVDVFAATLQYMGKNPNSTNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
CVALGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPEAALKWINYVSDPKVNAAITNTVFYPTAN
KAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLQNRLWAQLKTGH
>Mature_372_residues
MRACFLRQACSVAALAAAAAFTSVASPTAHAADELNVYNWSDYIAPDTIPNFQKQTGIHVKYDNYDSDDTLQAKLLAGSS
GYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPLLMKMIADADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPV
DSWALVFDPANMEKLKGCGVSFLDQAVDVFAATLQYMGKNPNSTNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
CVALGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPEAALKWINYVSDPKVNAAITNTVFYPTAN
KAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRLQNRLWAQLKTGH

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: COG0687

COG function: function code E; Spermidine/putrescine-binding periplasmic protein

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=361, Percent_Identity=49.0304709141274, Blast_Score=368, Evalue=1e-103,
Organism=Escherichia coli, GI1787367, Length=320, Percent_Identity=37.8125, Blast_Score=202, Evalue=2e-53,
Organism=Escherichia coli, GI1787711, Length=311, Percent_Identity=24.7588424437299, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40632; Mature: 40632

Theoretical pI: Translated: 7.11; Mature: 7.11

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRACFLRQACSVAALAAAAAFTSVASPTAHAADELNVYNWSDYIAPDTIPNFQKQTGIHV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECCCCCCCCCCCCCHHHHCCCEE
KYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPLLM
EECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KMIADADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVDSWALVFDPANMEKLKGCGV
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCH
SFLDQAVDVFAATLQYMGKNPNSTNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCE
CVALGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPEAALKWINY
EEEECCCCCCCCCCCCCCHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
VSDPKVNAAITNTVFYPTANKAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRL
HCCCCCCEEEEEEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
QNRLWAQLKTGH
HHHHHHHHCCCC
>Mature Secondary Structure
MRACFLRQACSVAALAAAAAFTSVASPTAHAADELNVYNWSDYIAPDTIPNFQKQTGIHV
CCHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHCCCEECCCCCCCCCCCCCHHHHCCCEE
KYDNYDSDDTLQAKLLAGSSGYDIVVPTSNYMAKQIQAGVYQKLDKSKLPNLANLDPLLM
EECCCCCCCCEEEEEEECCCCCEEEEECCHHHHHHHHHHHHHHHHHHHCCCCCCCCHHHH
KMIADADPGNQYGVPWAYGTDGIGYNAQAVKKALGDKAPVDSWALVFDPANMEKLKGCGV
HHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHCCCCCCCCEEEEECCCCHHHHHCCCH
SFLDQAVDVFAATLQYMGKNPNSTNPGDYQAAFEVLKKVRPYITQFNSSGYINDLANNDV
HHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHCCCCCE
CVALGWSGDVGIAHRRSAEAKRSYDIKFSNPKEGGLLWFDVMVIPKDAPHPEAALKWINY
EEEECCCCCCCCCCCCCCHHHHCCCEEECCCCCCCEEEEEEEEECCCCCCHHHHHHHHHH
VSDPKVNAAITNTVFYPTANKAAHQFVTPAVAQDPTVYPPEDVLKKMVLMKPMPADILRL
HCCCCCCEEEEEEEEECCCCHHHHHHHCCHHCCCCCCCCHHHHHHHHHHCCCCCHHHHHH
QNRLWAQLKTGH
HHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]