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The map label for this gene is purR [H]

Identifier: 78067790

GI number: 78067790

Start: 3487722

End: 3488741

Strand: Direct

Name: purR [H]

Synonym: Bcep18194_A6321

Alternate gene names: 78067790

Gene position: 3487722-3488741 (Clockwise)

Preceding gene: 78067789

Following gene: 78067791

Centisome position: 94.41

GC content: 72.16

Gene sequence:

>1020_bases
ATGGGTACGACCATTCGCGATGTGGCGCGGGCGGCAGAGGTCTCGATCGGCACCGTCTCCCGCGCGCTGAAAAACCAGCC
GGGCCTGTCCGAAGCGACGCGGGCGCGCATCGTCGAGATCGCGCAACGACTCGGCTACGATCCCGCGCAACTGCGGCCGC
GCATCCGCCGGCTCACCTTCCTGCTGCACCGCCAGCACAACCGCTTTCCGGCCAGCCCGTTCTTCTCGCACGTGCTGCAC
GGCGTCGAGGACGCGTGCCGCGAGCGCGGCATCGTGCCGACGCTGCTCACGGTCGGGCCGAACGACGACGTGCTGCGCCA
GATGCGCCCGCACGCGCCCGACGCGATCGCGGTGGCCGGCTTCATCGAGCCCGAGACGATCGAAGCGCTCGCCGCGACCG
GCCGGCCGCTCGTGCTGATCGACCTGTGGGCGCCCGGGCTGCGTTCGGTGAATATCGACAACGCGACGGGCGCGGCGCTC
GCGATGCGCCACCTGCTCGCCACCGGCCGCACGCGGATCGCGTTCATCGGCGGCTCGCCCGCGCACTACAGCATTGCGCA
GCGCGCGATCGGCTACCGCCGCGCGTTCTTCGAAGCCGGGCGGCTGTTCGATCCCGCGTACGAAGTGACGATCGACGCGG
GGCTCGATCCCGACACCGGTGCCTCGCGAGCGATGGAGCAGTTGCTCGACGCGCCGGGCCCGCGCCCGGAAGCCGTCTTC
GCGTACAACGACGCGGCCGCGCTCGCCGCGCAGCGCGTGTGCACCGCGCGCGGGCTGCGCATTCCCGACGACATCGCGAT
CGTCGGCTTCGACAACATCCCGGCCGCCGCGCACGCAAGCCCGCCGCTCACGACGCTCGCGGTCGACAAGGAGGCGCTCG
GCCGCCGCGGCGTCGAGCTGCTGCTCGCCGACTCGCCCGAGCGCACCGAGATTTCCCTGCCCGTCGAGCTGATCGTGCGG
GCCAGCAGCCAGCCCGCCGGCTCCCCGGCACTCGATACCGCCACGGTCACCGAATCATGA

Upstream 100 bases:

>100_bases
AACCGGGAATTCGCGGGTATTCCCCGGCTATCCGGCCCGAATTCCGCGATATGTTTTACTATACAATCGCTTTCACCCTA
AACAAACCGGACCCCGAACG

Downstream 100 bases:

>100_bases
ACATGCCCCCGGTCCAACCCTGCACGGCCGCGCCGGCCACCCACACCCAAGCCGCGCCGTTCGTCGCGAGTTTCCGCGAT
CCGTCGTTCCTGTTGTCGCA

Product: LacI family transcription regulator

Products: NA

Alternate protein names: Pur regulon repressor; Purine nucleotide synthesis repressor [H]

Number of amino acids: Translated: 339; Mature: 338

Protein sequence:

>339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRTRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGASRAMEQLLDAPGPRPEAVF
AYNDAAALAAQRVCTARGLRIPDDIAIVGFDNIPAAAHASPPLTTLAVDKEALGRRGVELLLADSPERTEISLPVELIVR
ASSQPAGSPALDTATVTES

Sequences:

>Translated_339_residues
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLH
GVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAAL
AMRHLLATGRTRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGASRAMEQLLDAPGPRPEAVF
AYNDAAALAAQRVCTARGLRIPDDIAIVGFDNIPAAAHASPPLTTLAVDKEALGRRGVELLLADSPERTEISLPVELIVR
ASSQPAGSPALDTATVTES
>Mature_338_residues
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTFLLHRQHNRFPASPFFSHVLHG
VEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAGFIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALA
MRHLLATGRTRIAFIGGSPAHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGASRAMEQLLDAPGPRPEAVFA
YNDAAALAAQRVCTARGLRIPDDIAIVGFDNIPAAAHASPPLTTLAVDKEALGRRGVELLLADSPERTEISLPVELIVRA
SSQPAGSPALDTATVTES

Specific function: Is the main repressor of the genes involved in the de novo synthesis of purine nucleotides, regulating purB, purC, purEK, purF, purHD, purL, purMN and guaBA expression. PurR is allosterically activated to bind its cognate DNA by binding the purine corepre

COG id: COG1609

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lacI-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790369, Length=351, Percent_Identity=32.4786324786325, Blast_Score=140, Evalue=8e-35,
Organism=Escherichia coli, GI1790194, Length=313, Percent_Identity=32.9073482428115, Blast_Score=125, Evalue=3e-30,
Organism=Escherichia coli, GI1789456, Length=316, Percent_Identity=29.746835443038, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787948, Length=313, Percent_Identity=29.3929712460064, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789202, Length=294, Percent_Identity=30.6122448979592, Blast_Score=105, Evalue=3e-24,
Organism=Escherichia coli, GI1788474, Length=328, Percent_Identity=29.5731707317073, Blast_Score=97, Evalue=1e-21,
Organism=Escherichia coli, GI48994940, Length=341, Percent_Identity=28.7390029325513, Blast_Score=86, Evalue=3e-18,
Organism=Escherichia coli, GI1787580, Length=315, Percent_Identity=26.984126984127, Blast_Score=85, Evalue=5e-18,
Organism=Escherichia coli, GI1789068, Length=298, Percent_Identity=25.8389261744966, Blast_Score=80, Evalue=2e-16,
Organism=Escherichia coli, GI1786540, Length=352, Percent_Identity=26.1363636363636, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787906, Length=269, Percent_Identity=28.2527881040892, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1790689, Length=327, Percent_Identity=24.1590214067278, Blast_Score=61, Evalue=1e-10,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000843
- InterPro:   IPR010982
- InterPro:   IPR001761 [H]

Pfam domain/function: PF00356 LacI; PF00532 Peripla_BP_1 [H]

EC number: NA

Molecular weight: Translated: 36280; Mature: 36149

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS00356 HTH_LACI_1 ; PS50932 HTH_LACI_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.6 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
1.8 %Cys+Met (Translated Protein)
0.6 %Cys     (Mature Protein)
0.9 %Met     (Mature Protein)
1.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MGTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTF
CCCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRTRIAFIGGSP
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCC
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGASRAMEQLLDAPGPRPEAVF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCTARGLRIPDDIAIVGFDNIPAAAHASPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCEEE
LLADSPERTEISLPVELIVRASSQPAGSPALDTATVTES
EEECCCCCCEECCCHHEEEECCCCCCCCCCCCCCCCCCC
>Mature Secondary Structure 
GTTIRDVARAAEVSIGTVSRALKNQPGLSEATRARIVEIAQRLGYDPAQLRPRIRRLTF
CCHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLHRQHNRFPASPFFSHVLHGVEDACRERGIVPTLLTVGPNDDVLRQMRPHAPDAIAVAG
HHHHHCCCCCCCHHHHHHHHHHHHHHHHCCCCEEEEEECCCHHHHHHHCCCCCCCEEEEE
FIEPETIEALAATGRPLVLIDLWAPGLRSVNIDNATGAALAMRHLLATGRTRIAFIGGSP
CCCHHHHHHHHHCCCCEEEEEECCCCCCEECCCCCCHHHHHHHHHHHCCCEEEEEECCCC
AHYSIAQRAIGYRRAFFEAGRLFDPAYEVTIDAGLDPDTGASRAMEQLLDAPGPRPEAVF
CHHHHHHHHHHHHHHHHHHCCCCCCCEEEEEECCCCCCCCHHHHHHHHHHCCCCCCCEEE
AYNDAAALAAQRVCTARGLRIPDDIAIVGFDNIPAAAHASPPLTTLAVDKEALGRRGVEL
EECCHHHHHHHHHHHHCCCCCCCCEEEEECCCCCCCCCCCCCCEEEEECHHHHCCCCEEE
LLADSPERTEISLPVELIVRASSQPAGSPALDTATVTES
EEECCCCCCEECCCHHEEEECCCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA