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The map label for this gene is yafC [C]

Identifier: 78067785

GI number: 78067785

Start: 3480775

End: 3481737

Strand: Reverse

Name: yafC [C]

Synonym: Bcep18194_A6316

Alternate gene names: 78067785

Gene position: 3481737-3480775 (Counterclockwise)

Preceding gene: 78067786

Following gene: 78067783

Centisome position: 94.25

GC content: 66.98

Gene sequence:

>963_bases
ATGGATACCCTCGTCAGCATGAATGTGTTTCGCTACGTCGTCGAAGTGGGCAGCTTCGTCGGCGCGGCCGAGCGCATGCA
GATGTCGGCTGCGATGGCGAGCAAGCACGTGATGCATCTCGAGCAGCAGCTCGGTGCGCGGCTGCTGCATCGCACGACAC
GACGCGTCGCCCCTACCGAGGCAGGACGCGAATACTATGAGCGCCTCGTGCAGGCGCTGTCTGAACTCGACGAAGCCGGG
CAGGCCGTCGGCGCCGCGAGCGTGGTGCCGCAAGGCAGGCTGCGCGTCACGTCGCTATCGGCATTCGGGCTGCGGCACGT
GATGCAGGCCGTCACCGATTATGCGGGCCGGTTCCCGGACGTGACCGTCGACATGACGCTGTCCGATCGCGTGGTCGACC
TGATCGAGGAAGGCTACGACGTCGCGGTGCGTGCGGCGCCGAACGGATTGAAATCGTCGTCGCTGGTTGCGCGGCAGATT
GCGACCGCGCACATCCTGCTGGTCGCGTCGCCCGAGTATCTCGAGAAGCACGGCACGCCCGCGACGATCGCCGATCTCGC
GCATCACAACTACCTGCGGCGCGACTCGAATTCGACGATGCTCGACTCGCTGGTGATCGACGCGGCCGCCGCGTCGCGCG
TGAACCTCAACGGCAACCTGATCGTCAATCATCTCGAAGGGCTGCGTGCGGCCGTGCTCGCGGGCGCGGGCATCGCGCTG
CTCGGCACCGAGGTGGTCGGCGACGACATCGAGTCCGGCCGGCTCGTGCCGGTGCTGCTCGATGCGGTGCCGCCGCACGA
AGCGCCGATCTATGCGGTCTACGCGAGCCGTCGTCACGTGTCCGCGAAGGTGCGGTCGTTCGTCGATTTCCTGGCGGCGC
GTTTCGAAGGGCAGTCGCTGTGCCCGTCGATCGAATCGCGCCTGCGCGTGCTGCCGATCACGCGGATGAAGCGCGCGGTC
TGA

Upstream 100 bases:

>100_bases
CCCCGCGGCGCCGTTTTGCCTTTCTTGACCTTTCTGTTTCCCGGCCTTTGTTACCCTATCTCGTAATTGGACCATTCCCC
CGTCATTACGAGCTAGTTCG

Downstream 100 bases:

>100_bases
GCCGTCTTTCCCGTGGCAGTGGGGCAATAAAAAAGCGCGAACCGCTGCGGTTCGCGCTTTTTGTTTGGTCCGACGGAATG
CGTGCCGGCGGCGGCCGTGA

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: ORF2 [H]

Number of amino acids: Translated: 320; Mature: 320

Protein sequence:

>320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPATIADLAHHNYLRRDSNSTMLDSLVIDAAAASRVNLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPVLLDAVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV

Sequences:

>Translated_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPATIADLAHHNYLRRDSNSTMLDSLVIDAAAASRVNLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPVLLDAVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV
>Mature_320_residues
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTEAGREYYERLVQALSELDEAG
QAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPDVTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQI
ATAHILLVASPEYLEKHGTPATIADLAHHNYLRRDSNSTMLDSLVIDAAAASRVNLNGNLIVNHLEGLRAAVLAGAGIAL
LGTEVVGDDIESGRLVPVLLDAVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSLCPSIESRLRVLPITRMKRAV

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1786401, Length=286, Percent_Identity=34.965034965035, Blast_Score=166, Evalue=1e-42,
Organism=Escherichia coli, GI87081978, Length=299, Percent_Identity=36.1204013377926, Blast_Score=149, Evalue=3e-37,
Organism=Escherichia coli, GI1789639, Length=296, Percent_Identity=31.0810810810811, Blast_Score=136, Evalue=2e-33,
Organism=Escherichia coli, GI1789440, Length=290, Percent_Identity=30.3448275862069, Blast_Score=125, Evalue=2e-30,
Organism=Escherichia coli, GI1787128, Length=294, Percent_Identity=29.5918367346939, Blast_Score=123, Evalue=2e-29,
Organism=Escherichia coli, GI145693193, Length=299, Percent_Identity=29.7658862876254, Blast_Score=121, Evalue=8e-29,
Organism=Escherichia coli, GI1787589, Length=299, Percent_Identity=27.0903010033445, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1789173, Length=298, Percent_Identity=27.5167785234899, Blast_Score=84, Evalue=9e-18,
Organism=Escherichia coli, GI1788706, Length=185, Percent_Identity=26.4864864864865, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1788481, Length=294, Percent_Identity=22.108843537415, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1790011, Length=295, Percent_Identity=23.728813559322, Blast_Score=64, Evalue=1e-11,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 34640; Mature: 34640

Theoretical pI: Translated: 7.43; Mature: 7.43

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
VTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQIATAHILLVASPEYLEKHGTP
EEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCC
ATIADLAHHNYLRRDSNSTMLDSLVIDAAAASRVNLNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEECCCCCEEEEHHHHHHHHHHHCCCHHH
LGTEVVGDDIESGRLVPVLLDAVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRLRVLPITRMKRAV
CHHHHHCCEECCHHHHHHCC
>Mature Secondary Structure
MDTLVSMNVFRYVVEVGSFVGAAERMQMSAAMASKHVMHLEQQLGARLLHRTTRRVAPTE
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCH
AGREYYERLVQALSELDEAGQAVGAASVVPQGRLRVTSLSAFGLRHVMQAVTDYAGRFPD
HHHHHHHHHHHHHHHHHHHHHHHCHHHCCCCCCEEEEHHHHHHHHHHHHHHHHHHCCCCC
VTVDMTLSDRVVDLIEEGYDVAVRAAPNGLKSSSLVARQIATAHILLVASPEYLEKHGTP
EEEEEEHHHHHHHHHHCCCCEEEEECCCCCCHHHHHHHHHHHHEEEEEECHHHHHHCCCC
ATIADLAHHNYLRRDSNSTMLDSLVIDAAAASRVNLNGNLIVNHLEGLRAAVLAGAGIAL
HHHHHHHHHHHHHCCCCCHHHHHHHHHHHHHCEECCCCCEEEEHHHHHHHHHHHCCCHHH
LGTEVVGDDIESGRLVPVLLDAVPPHEAPIYAVYASRRHVSAKVRSFVDFLAARFEGQSL
HHHHHHCCCCCCCCEEEEEEECCCCCCCCEEEEEECCHHHHHHHHHHHHHHHHHCCCCCC
CPSIESRLRVLPITRMKRAV
CHHHHHCCEECCHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8635745; 7542800 [H]