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The map label for this gene is ghrA [H]

Identifier: 78067777

GI number: 78067777

Start: 3474105

End: 3475046

Strand: Direct

Name: ghrA [H]

Synonym: Bcep18194_A6308

Alternate gene names: 78067777

Gene position: 3474105-3475046 (Clockwise)

Preceding gene: 78067775

Following gene: 78067778

Centisome position: 94.04

GC content: 71.97

Gene sequence:

>942_bases
ATGAAGATCCTGTTCCACTCCCCGCACCAGGAAGCCAGCGCGTGGCGCGACGAGATCGCGCATGCGCTGCCGGAAGCCGA
ATTGCGCGCGTGGCAGCCGGGCGACACGGCCGCCGCCGACTACGCGCTCGTCTGGCGCGCGCCGCGCGAATTCTTCGCCC
CGCGCGACGGCCTGCGCGCGATCTTCAACCTCGGCGCCGGCGTCGACGCGCTGCTCGCGCTCGAGCACGCGCATCCGGGC
ACGCTGCCGCGGCACGTACCGCTCGTGCGGCTGGAAGATTCGGGCATGGCGCAGCAGATGGTCGAATACGTGGCGCACGC
GGTGCTGCGCTACCTGCGCCGCTTCGACGAATACGAAGTGCTGCAGCGCGAGCGGCGCTGGCAGCCGCTCGAACCGCATG
CGCGCGCACGCTTCACCGTCGCCGTGCTCGGCCTCGGCGTGCTCGGCGCGCAGGTCGCGCGCGCGCTCGCCGCGCTCGGC
CTGCCCGTGCGCGGCTACAGCCGCAGCGCGAAGCAGCTCGACGGCGTCACGACCTTCGCCGGCGAAGGCGCATTCGATGC
GTGCATCGACGGCGCAAAGGTGCTCGTCAACCTGCTGCCGAGCACGCCCGACACCGACGGCATCCTGTCGTCGCGCGCGT
TCGCGCGGCTCGCGCCCGGTGCGTATGTCGTCAACGTCGCACGCGGCGCGCACCTCGTAGAAGCCGATCTGCTCGACGCA
CTCTCGAGCGGCCAGGTAGCCGCCGCGACGCTCGACGTGTTCCATCGCGAGCCGCTGCCGGAAGACCATCCGTTCTGGCA
CGCGCCGCGCATCACGATCACGCCGCACAGCTCGGCCGAGACGCTGCGCGACGAGGCCATCGCGCAGATCGCCGGCAAGA
TCCGCGCGCTCGAGCGCGGCGAGCGCGTCGGCGGCATCGTCGACTACGCACGCGGCTACTGA

Upstream 100 bases:

>100_bases
TCAGGAGACGCCCGATGTCCGCTACGCCCGCCGGCGCGCTGCGCCCGGCCCCGCTCGCCGTTCCCGTGACGCGCCTCGCC
GGCGCGCGCGCCGTCATCCA

Downstream 100 bases:

>100_bases
ACCTACAAGCAAACCAGGAGACAACCATGACGTTCCCCACCGCCGTCAAGATCGTCGAAGTCGGCCCGCGCGACGGGCTG
CAGAACGAGAAGACCTTCGT

Product: D-isomer specific 2-hydroxyacid dehydrogenase, NAD-binding

Products: NA

Alternate protein names: 2-ketoacid reductase [H]

Number of amino acids: Translated: 313; Mature: 313

Protein sequence:

>313_residues
MKILFHSPHQEASAWRDEIAHALPEAELRAWQPGDTAAADYALVWRAPREFFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPRHVPLVRLEDSGMAQQMVEYVAHAVLRYLRRFDEYEVLQRERRWQPLEPHARARFTVAVLGLGVLGAQVARALAALG
LPVRGYSRSAKQLDGVTTFAGEGAFDACIDGAKVLVNLLPSTPDTDGILSSRAFARLAPGAYVVNVARGAHLVEADLLDA
LSSGQVAAATLDVFHREPLPEDHPFWHAPRITITPHSSAETLRDEAIAQIAGKIRALERGERVGGIVDYARGY

Sequences:

>Translated_313_residues
MKILFHSPHQEASAWRDEIAHALPEAELRAWQPGDTAAADYALVWRAPREFFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPRHVPLVRLEDSGMAQQMVEYVAHAVLRYLRRFDEYEVLQRERRWQPLEPHARARFTVAVLGLGVLGAQVARALAALG
LPVRGYSRSAKQLDGVTTFAGEGAFDACIDGAKVLVNLLPSTPDTDGILSSRAFARLAPGAYVVNVARGAHLVEADLLDA
LSSGQVAAATLDVFHREPLPEDHPFWHAPRITITPHSSAETLRDEAIAQIAGKIRALERGERVGGIVDYARGY
>Mature_313_residues
MKILFHSPHQEASAWRDEIAHALPEAELRAWQPGDTAAADYALVWRAPREFFAPRDGLRAIFNLGAGVDALLALEHAHPG
TLPRHVPLVRLEDSGMAQQMVEYVAHAVLRYLRRFDEYEVLQRERRWQPLEPHARARFTVAVLGLGVLGAQVARALAALG
LPVRGYSRSAKQLDGVTTFAGEGAFDACIDGAKVLVNLLPSTPDTDGILSSRAFARLAPGAYVVNVARGAHLVEADLLDA
LSSGQVAAATLDVFHREPLPEDHPFWHAPRITITPHSSAETLRDEAIAQIAGKIRALERGERVGGIVDYARGY

Specific function: Catalyzes the NADPH-dependent reduction of glyoxylate and hydroxypyruvate into glycolate and glycerate, respectively [H]

COG id: COG0111

COG function: function code HE; Phosphoglycerate dehydrogenase and related dehydrogenases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the D-isomer specific 2-hydroxyacid dehydrogenase family. GhrA subfamily [H]

Homologues:

Organism=Homo sapiens, GI23308577, Length=265, Percent_Identity=28.3018867924528, Blast_Score=82, Evalue=5e-16,
Organism=Homo sapiens, GI6912396, Length=256, Percent_Identity=27.34375, Blast_Score=79, Evalue=8e-15,
Organism=Escherichia coli, GI87081824, Length=313, Percent_Identity=49.5207667731629, Blast_Score=310, Evalue=6e-86,
Organism=Escherichia coli, GI87082289, Length=264, Percent_Identity=25, Blast_Score=62, Evalue=4e-11,
Organism=Caenorhabditis elegans, GI17532191, Length=172, Percent_Identity=28.4883720930233, Blast_Score=71, Evalue=6e-13,
Organism=Saccharomyces cerevisiae, GI6322116, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=3e-12,
Organism=Saccharomyces cerevisiae, GI6320925, Length=190, Percent_Identity=27.8947368421053, Blast_Score=67, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24585516, Length=146, Percent_Identity=30.8219178082192, Blast_Score=73, Evalue=2e-13,
Organism=Drosophila melanogaster, GI28571528, Length=138, Percent_Identity=29.7101449275362, Blast_Score=68, Evalue=7e-12,
Organism=Drosophila melanogaster, GI19921140, Length=146, Percent_Identity=32.1917808219178, Blast_Score=67, Evalue=2e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006140
- InterPro:   IPR016040 [H]

Pfam domain/function: PF02826 2-Hacid_dh_C [H]

EC number: =1.1.1.79; =1.1.1.81 [H]

Molecular weight: Translated: 34167; Mature: 34167

Theoretical pI: Translated: 6.71; Mature: 6.71

Prosite motif: PS00671 D_2_HYDROXYACID_DH_3

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.0 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKILFHSPHQEASAWRDEIAHALPEAELRAWQPGDTAAADYALVWRAPREFFAPRDGLRA
CEEEECCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCEEEECCHHHHCCCHHHHH
IFNLGAGVDALLALEHAHPGTLPRHVPLVRLEDSGMAQQMVEYVAHAVLRYLRRFDEYEV
HHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LQRERRWQPLEPHARARFTVAVLGLGVLGAQVARALAALGLPVRGYSRSAKQLDGVTTFA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEC
GEGAFDACIDGAKVLVNLLPSTPDTDGILSSRAFARLAPGAYVVNVARGAHLVEADLLDA
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LSSGQVAAATLDVFHREPLPEDHPFWHAPRITITPHSSAETLRDEAIAQIAGKIRALERG
HCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ERVGGIVDYARGY
CHHCCHHHHHCCC
>Mature Secondary Structure
MKILFHSPHQEASAWRDEIAHALPEAELRAWQPGDTAAADYALVWRAPREFFAPRDGLRA
CEEEECCCCHHHHHHHHHHHHHCCHHHHHCCCCCCCCHHCCCEEEECCHHHHCCCHHHHH
IFNLGAGVDALLALEHAHPGTLPRHVPLVRLEDSGMAQQMVEYVAHAVLRYLRRFDEYEV
HHHCCCCHHHHHHHHCCCCCCCCCCCCEEEECCCCHHHHHHHHHHHHHHHHHHHCCHHHH
LQRERRWQPLEPHARARFTVAVLGLGVLGAQVARALAALGLPVRGYSRSAKQLDGVTTFA
HHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHCCCCEEC
GEGAFDACIDGAKVLVNLLPSTPDTDGILSSRAFARLAPGAYVVNVARGAHLVEADLLDA
CCCCHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHH
LSSGQVAAATLDVFHREPLPEDHPFWHAPRITITPHSSAETLRDEAIAQIAGKIRALERG
HCCCCCHHHHHHHHHCCCCCCCCCCCCCCEEEECCCCCHHHHHHHHHHHHHHHHHHHHHC
ERVGGIVDYARGY
CHHCCHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA