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The map label for this gene is pycA [H]

Identifier: 78067751

GI number: 78067751

Start: 3443374

End: 3444837

Strand: Direct

Name: pycA [H]

Synonym: Bcep18194_A6282

Alternate gene names: 78067751

Gene position: 3443374-3444837 (Clockwise)

Preceding gene: 78067750

Following gene: 78067752

Centisome position: 93.21

GC content: 71.17

Gene sequence:

>1464_bases
ATGAAACCGGCAACTTCCCCCGACACGTTCTACCGCCCGTCGCGGATCCGGACCGTGCTGGTCGCGAACCGCGGCGAGAT
CGCGGTGCGTGTGATCCGCGCCGCGCGCGAGCTCGGCATGCGCGCGGTCACCGTGGTCAGCGACGCGGACCGCGACAGCC
TCGCCGCGCGCATGGCCGACGACGCCATCCACATCGGCTCGTCGCACGCGGCGAAAAGCTACCTGAATCCCCCCGCGATC
CTCGACGCCGCGCGGCAGTGCGGCGCGGACGCGATCCATCCCGGCTACGGCTTCCTGTCCGAAAACGCCGCATTCGCCGC
GCAGGTCGAAGCGGCCGGCCTGATCTTCGTCGGCCCGGATTCGCAGGTGATCGCCACGATGGGCGACAAGGCCCGCGCAC
GCGAAACCGCGCGGCGCGCCAACGTGCCGACCGTGCCCGGCAGCGACGGCGTCGTGCAATCGCTCGACGAGGCACGGGAC
GTCGCCGCGCGCATCGGCTACCCGATCATGATCAAGGCAGCCGCCGGCGGCGGCGGGCGCGGCATCCGCGTCGCGCACGA
CGCCGCGCAGCTCGACGCCGAGCTGCCGCTCGCGCAGCGCGAGGCGCAGGCCGCGTTCGGCGACGGCGGCGTGTACCTCG
AACGCTTCATCGCACGGGCGCGCCACATCGAGGTGCAGGTGCTCGGCGACGGCCGCAACGTCGTCCACCTGTTCGAGCGC
GAATGCTCGCTGCAGCGGCGGCGCCAAAAAATCCTCGAGGAGGCGCCGTCGCCTTCGCTCACGCCCGCGCAACGCGACGC
GCTGTGCGCGTCGGCCACACGCCTCGCGCAAGAAGTCGGCTATCGCAGCGCGGGCACGCTCGAATACCTGTTCGACGACG
CACGCGGCGAGTTCTACTTTATCGAGATGAACACGCGCATCCAGGTCGAGCATCCGGTGACCGAAGCGATCACCGGCATC
GATCTCGTGCGCGAGACGCTGCGCATCGCCGACGGCGAGCCGCTGCGCTTCGCGCAGCAGGACATCGCGATGCGCGGCGC
GGCGCTCGAATGCCGGATCAACGCGGAAGATCCGCTGCAGGATTTCCGCCCGAACCCCGGCCGGATCGACGAACTCGTGT
GGCCGACCGGCCCCGGCGTGCGCGTCGATTCGCTGCTCTACCCGGGCTACGTCGTGCCGCCGTTCTACGATTCGCTGCTC
GCGAAGCTGATCGTGCACGACGAGAGCCGTGCGGCCGCGCTGCGGCGGATGGCCCGCGCGCTGGGCGAGCTGCACGTCGG
CGGCCTGAAGACGACCGCGCCGCTGCACGTCGCGCTGCTCGCGGACGACGACGTGCGCGCCGGGCGCTATCACACGAATT
TCCTCGAGGCGTGGATGCCGCGCTGGCGCGAAGCCGCCACCGCCGCCGCGCAACGACACGCAACCGACAATGCAGACGCC
GTGCGCGTCGGGGAGGCGATGTGA

Upstream 100 bases:

>100_bases
ATCGAAACCGATGCGGCCGGGCGCGTGGTCGAGCTGCTCGTCGAGGACGGCGAGCCGGTCGACGCGGGCCAGGTACTGAT
GCGGATCGAGGGGTGATGCG

Downstream 100 bases:

>100_bases
CGACCCGTTATACGTTCGGCGGCGACGAGTTCGTGTTCGTCGAAATCAGCGAATCGATGTCGCTCGATGCGTTCTTCAAG
GGCACGGCAATCACGCGCGA

Product: acetyl-CoA carboxylase biotin carboxylase subunit

Products: NA

Alternate protein names: Pyruvic carboxylase A [H]

Number of amino acids: Translated: 487; Mature: 487

Protein sequence:

>487_residues
MKPATSPDTFYRPSRIRTVLVANRGEIAVRVIRAARELGMRAVTVVSDADRDSLAARMADDAIHIGSSHAAKSYLNPPAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARETARRANVPTVPGSDGVVQSLDEARD
VAARIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRNVVHLFER
ECSLQRRRQKILEEAPSPSLTPAQRDALCASATRLAQEVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDELVWPTGPGVRVDSLLYPGYVVPPFYDSLL
AKLIVHDESRAAALRRMARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMPRWREAATAAAQRHATDNADA
VRVGEAM

Sequences:

>Translated_487_residues
MKPATSPDTFYRPSRIRTVLVANRGEIAVRVIRAARELGMRAVTVVSDADRDSLAARMADDAIHIGSSHAAKSYLNPPAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARETARRANVPTVPGSDGVVQSLDEARD
VAARIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRNVVHLFER
ECSLQRRRQKILEEAPSPSLTPAQRDALCASATRLAQEVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDELVWPTGPGVRVDSLLYPGYVVPPFYDSLL
AKLIVHDESRAAALRRMARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMPRWREAATAAAQRHATDNADA
VRVGEAM
>Mature_487_residues
MKPATSPDTFYRPSRIRTVLVANRGEIAVRVIRAARELGMRAVTVVSDADRDSLAARMADDAIHIGSSHAAKSYLNPPAI
LDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPDSQVIATMGDKARARETARRANVPTVPGSDGVVQSLDEARD
VAARIGYPIMIKAAAGGGGRGIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRNVVHLFER
ECSLQRRRQKILEEAPSPSLTPAQRDALCASATRLAQEVGYRSAGTLEYLFDDARGEFYFIEMNTRIQVEHPVTEAITGI
DLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQDFRPNPGRIDELVWPTGPGVRVDSLLYPGYVVPPFYDSLL
AKLIVHDESRAAALRRMARALGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMPRWREAATAAAQRHATDNADA
VRVGEAM

Specific function: Pyruvate carboxylase catalyzes a 2-step reaction, involving the ATP-dependent carboxylation of the covalently attached biotin in the first step and the transfer of the carboxyl group to pyruvate in the second [H]

COG id: COG0439

COG function: function code I; Biotin carboxylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 biotin carboxylation domain [H]

Homologues:

Organism=Homo sapiens, GI295821183, Length=445, Percent_Identity=47.4157303370787, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI65506442, Length=445, Percent_Identity=47.4157303370787, Blast_Score=393, Evalue=1e-109,
Organism=Homo sapiens, GI189095269, Length=445, Percent_Identity=47.4157303370787, Blast_Score=392, Evalue=1e-109,
Organism=Homo sapiens, GI106049528, Length=463, Percent_Identity=44.9244060475162, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI106049295, Length=463, Percent_Identity=44.9244060475162, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI106049292, Length=463, Percent_Identity=44.9244060475162, Blast_Score=375, Evalue=1e-104,
Organism=Homo sapiens, GI116805327, Length=442, Percent_Identity=44.3438914027149, Blast_Score=369, Evalue=1e-102,
Organism=Homo sapiens, GI38679977, Length=503, Percent_Identity=32.6043737574553, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI38679967, Length=503, Percent_Identity=32.6043737574553, Blast_Score=239, Evalue=3e-63,
Organism=Homo sapiens, GI38679971, Length=503, Percent_Identity=32.6043737574553, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI38679960, Length=503, Percent_Identity=32.6043737574553, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI38679974, Length=503, Percent_Identity=32.6043737574553, Blast_Score=239, Evalue=4e-63,
Organism=Homo sapiens, GI134142062, Length=511, Percent_Identity=32.2896281800391, Blast_Score=230, Evalue=2e-60,
Organism=Escherichia coli, GI1789654, Length=443, Percent_Identity=50.7900677200903, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1786216, Length=297, Percent_Identity=23.9057239057239, Blast_Score=62, Evalue=9e-11,
Organism=Caenorhabditis elegans, GI17567343, Length=453, Percent_Identity=45.916114790287, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI71987519, Length=454, Percent_Identity=46.9162995594714, Blast_Score=382, Evalue=1e-106,
Organism=Caenorhabditis elegans, GI17562816, Length=451, Percent_Identity=44.1241685144124, Blast_Score=360, Evalue=1e-99,
Organism=Caenorhabditis elegans, GI71997168, Length=509, Percent_Identity=28.6836935166994, Blast_Score=195, Evalue=4e-50,
Organism=Caenorhabditis elegans, GI133931226, Length=487, Percent_Identity=28.1314168377823, Blast_Score=194, Evalue=9e-50,
Organism=Caenorhabditis elegans, GI71997163, Length=509, Percent_Identity=28.6836935166994, Blast_Score=191, Evalue=5e-49,
Organism=Saccharomyces cerevisiae, GI6319685, Length=442, Percent_Identity=42.9864253393665, Blast_Score=374, Evalue=1e-104,
Organism=Saccharomyces cerevisiae, GI6319695, Length=457, Percent_Identity=44.2013129102845, Blast_Score=354, Evalue=1e-98,
Organism=Saccharomyces cerevisiae, GI6321376, Length=457, Percent_Identity=44.4201312910284, Blast_Score=350, Evalue=4e-97,
Organism=Saccharomyces cerevisiae, GI6324343, Length=512, Percent_Identity=32.2265625, Blast_Score=224, Evalue=2e-59,
Organism=Saccharomyces cerevisiae, GI6323863, Length=460, Percent_Identity=31.304347826087, Blast_Score=214, Evalue=3e-56,
Organism=Drosophila melanogaster, GI281363050, Length=462, Percent_Identity=45.8874458874459, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652224, Length=462, Percent_Identity=45.8874458874459, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652222, Length=462, Percent_Identity=45.8874458874459, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652220, Length=462, Percent_Identity=45.8874458874459, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652218, Length=462, Percent_Identity=45.8874458874459, Blast_Score=382, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652212, Length=462, Percent_Identity=45.8874458874459, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652210, Length=462, Percent_Identity=45.8874458874459, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652214, Length=462, Percent_Identity=45.8874458874459, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI19921944, Length=462, Percent_Identity=45.8874458874459, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24652216, Length=462, Percent_Identity=45.8874458874459, Blast_Score=381, Evalue=1e-106,
Organism=Drosophila melanogaster, GI24651757, Length=455, Percent_Identity=44.1758241758242, Blast_Score=365, Evalue=1e-101,
Organism=Drosophila melanogaster, GI24651759, Length=405, Percent_Identity=42.962962962963, Blast_Score=320, Evalue=1e-87,
Organism=Drosophila melanogaster, GI24586458, Length=499, Percent_Identity=31.2625250501002, Blast_Score=219, Evalue=2e-57,
Organism=Drosophila melanogaster, GI161076409, Length=499, Percent_Identity=31.2625250501002, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI161076407, Length=499, Percent_Identity=31.2625250501002, Blast_Score=219, Evalue=3e-57,
Organism=Drosophila melanogaster, GI24586460, Length=499, Percent_Identity=31.2625250501002, Blast_Score=219, Evalue=3e-57,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004549
- InterPro:   IPR011761
- InterPro:   IPR013815
- InterPro:   IPR013816
- InterPro:   IPR011764
- InterPro:   IPR005482
- InterPro:   IPR005479
- InterPro:   IPR005481
- InterPro:   IPR013817
- InterPro:   IPR016185
- InterPro:   IPR011054 [H]

Pfam domain/function: PF02785 Biotin_carb_C; PF00289 CPSase_L_chain; PF02786 CPSase_L_D2 [H]

EC number: =6.4.1.1 [H]

Molecular weight: Translated: 52857; Mature: 52857

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: PS50975 ATP_GRASP ; PS00866 CPSASE_1 ; PS00867 CPSASE_2 ; PS50979 BC

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
2.1 %Met     (Translated Protein)
2.9 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
2.1 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPATSPDTFYRPSRIRTVLVANRGEIAVRVIRAARELGMRAVTVVSDADRDSLAARMAD
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
DAIHIGSSHAAKSYLNPPAILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPD
CCEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCC
SQVIATMGDKARARETARRANVPTVPGSDGVVQSLDEARDVAARIGYPIMIKAAAGGGGR
CCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRNVVHLFER
CEEEECHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHH
ECSLQRRRQKILEEAPSPSLTPAQRDALCASATRLAQEVGYRSAGTLEYLFDDARGEFYF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCEEE
IEMNTRIQVEHPVTEAITGIDLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQ
EEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
DFRPNPGRIDELVWPTGPGVRVDSLLYPGYVVPPFYDSLLAKLIVHDESRAAALRRMARA
HCCCCCCCCCCCCCCCCCCCEECCEECCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMPRWREAATAAAQRHATDNADA
HHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VRVGEAM
EECCCCC
>Mature Secondary Structure
MKPATSPDTFYRPSRIRTVLVANRGEIAVRVIRAARELGMRAVTVVSDADRDSLAARMAD
CCCCCCCCCCCCCCCEEEEEEECCCHHHHHHHHHHHHCCCEEEEEECCCCHHHHHHHHHC
DAIHIGSSHAAKSYLNPPAILDAARQCGADAIHPGYGFLSENAAFAAQVEAAGLIFVGPD
CCEECCCCHHHHHHCCCHHHHHHHHHCCCCCCCCCCCCCCCCCCEEEEEEECEEEEECCC
SQVIATMGDKARARETARRANVPTVPGSDGVVQSLDEARDVAARIGYPIMIKAAAGGGGR
CCEEEECCCHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHCCCCEEEEEECCCCCC
GIRVAHDAAQLDAELPLAQREAQAAFGDGGVYLERFIARARHIEVQVLGDGRNVVHLFER
CEEEECHHHHHCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHEEEEEEEECCCCCHHHHHH
ECSLQRRRQKILEEAPSPSLTPAQRDALCASATRLAQEVGYRSAGTLEYLFDDARGEFYF
HHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHEECCCCCEEE
IEMNTRIQVEHPVTEAITGIDLVRETLRIADGEPLRFAQQDIAMRGAALECRINAEDPLQ
EEECCEEEECCCHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCEEEEEECCCCCHH
DFRPNPGRIDELVWPTGPGVRVDSLLYPGYVVPPFYDSLLAKLIVHDESRAAALRRMARA
HCCCCCCCCCCCCCCCCCCCEECCEECCCEECCHHHHHHHHHHHHCCCHHHHHHHHHHHH
LGELHVGGLKTTAPLHVALLADDDVRAGRYHTNFLEAWMPRWREAATAAAQRHATDNADA
HHHHCCCCCCCCCCEEEEEEECCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCE
VRVGEAM
EECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 9371463; 9478969 [H]