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The map label for this gene is bioB

Identifier: 78067747

GI number: 78067747

Start: 3440049

End: 3441071

Strand: Direct

Name: bioB

Synonym: Bcep18194_A6278

Alternate gene names: 78067747

Gene position: 3440049-3441071 (Clockwise)

Preceding gene: 78067746

Following gene: 78067749

Centisome position: 93.12

GC content: 68.23

Gene sequence:

>1023_bases
ATGACCCAAGCCCAGACTGCCGCCACCGTGCAACCCGACGCGATTCCCGTGGCCGCTCCGGTCTCGCAGCGCTGGCGCGT
CGCCGACGTCGTCGCGCTGTTCGAGCTGCCGTTCAACGACCTGCTGTTCCGCGCGCAGCAGGTGCACCGCGAGCACTTCG
ACGCGAACGCGGTGCAGCTGTCGACGCTGCTGTCGATCAAGACGGGCGGCTGCGAGGAAGATTGCGGCTACTGCTCGCAG
TCGTCGCACCACGACACGGGCCTGAAGGCCGAGAAGCTGATGGACGTCGACGCGGTGCTCGATGCCGCGCGCGCGGCGAA
GGCGAACGGCGCGAGCCGCTTCTGCATGGGCGCCGCGTGGCGCAACCCGAAGGAGCGCCACATGCCGGCGCTGACGGAGA
TGGTGCGCGGCGTGAAGGAACTCGGCCTCGAGACCTGCATGACGCTCGGCATGCTGGAAGACGAGCAGGCGCAGGAACTC
GCGAGCGCGGGCCTCGACTACTACAACCACAACCTCGACACGTCGCCGGAGTTCTACGGCCAGGTGATCTCGACGCGCAC
CTACCAGGATCGCCTCGACACGCTCGACCGCGTGCGTGACGCGGGCATCAACGTGTGCTGCGGCGGCATCATCGGGATGG
GCGAATCGCGCCGCGAACGCGCGGGCCTGATCTCGCAGCTCGCGAACCTGAACCCGTATCCGGATTCGGTGCCGATCAAC
AACCTCGTCGCGATCGAAGGCACGCCGCTCGAAGGCACCGCGCCGCTCGACCCGTTCGAATTCGTGCGCACGATCGCGGT
GGCGCGGATCACGATGCCGAAGGCCGTCGTGCGCCTGTCGGCCGGCCGCGAGCAGCTCGACGACGGCCTGCAGGCGATGT
GCTTCCTCGCCGGCGCGAACTCGATGTTCTACGGCGACCAGCTGCTGACGACGAGCAACCCGCAGTCGCAGAAGGACCGT
GCGCTGTTCGAGCGCCTCGGCATCCGGTCGAGCGATGCGGACGCGATGTCGGCGAACGCGTAA

Upstream 100 bases:

>100_bases
GACATCGCCGCGCTCGTCGAGACGCTGCGCACCGCGCAGCCTTGACCCAACAGAAACAGATCCCCCGATCCCTCATCCCC
GACCAGGACAGGAAAACGAT

Downstream 100 bases:

>100_bases
GCGCCACGCACGGCGGATTCGTTCGCCGCATGAAAAAGCCGGGATACGTCCCGGCTTTTTTTACGTGTGCGCCGCGCACT
CAGCCCGCCACGCCCAACGC

Product: biotin synthase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 340; Mature: 339

Protein sequence:

>340_residues
MTQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQ
SSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQEL
ASAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPIN
NLVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDR
ALFERLGIRSSDADAMSANA

Sequences:

>Translated_340_residues
MTQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQ
SSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQEL
ASAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPIN
NLVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDR
ALFERLGIRSSDADAMSANA
>Mature_339_residues
TQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQLSTLLSIKTGGCEEDCGYCSQS
SHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAWRNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELA
SAGLDYYNHNLDTSPEFYGQVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPINN
LVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGANSMFYGDQLLTTSNPQSQKDRA
LFERLGIRSSDADAMSANA

Specific function: Catalyzes the conversion of dethiobiotin (DTB) to biotin by the insertion of a sulfur atom into dethiobiotin via a radical- based mechanism

COG id: COG0502

COG function: function code H; Biotin synthase and related enzymes

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the radical SAM superfamily. Biotin synthase family

Homologues:

Organism=Escherichia coli, GI1786992, Length=305, Percent_Identity=65.9016393442623, Blast_Score=437, Evalue=1e-124,
Organism=Saccharomyces cerevisiae, GI6321725, Length=320, Percent_Identity=48.125, Blast_Score=324, Evalue=1e-89,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): BIOB_BURS3 (Q39CE4)

Other databases:

- EMBL:   CP000151
- RefSeq:   YP_370516.1
- ProteinModelPortal:   Q39CE4
- SMR:   Q39CE4
- STRING:   Q39CE4
- GeneID:   3751511
- GenomeReviews:   CP000151_GR
- KEGG:   bur:Bcep18194_A6278
- NMPDR:   fig|269483.3.peg.7097
- eggNOG:   COG0502
- HOGENOM:   HBG405569
- OMA:   MGMETCM
- ProtClustDB:   CLSK2310607
- BioCyc:   BSP36773:BCEP18194_A6278-MONOMER
- HAMAP:   MF_01694
- InterPro:   IPR013785
- InterPro:   IPR010722
- InterPro:   IPR002684
- InterPro:   IPR006638
- InterPro:   IPR007197
- Gene3D:   G3DSA:3.20.20.70
- SMART:   SM00876
- SMART:   SM00729
- TIGRFAMs:   TIGR00433

Pfam domain/function: PF06968 BATS; PF04055 Radical_SAM

EC number: =2.8.1.6

Molecular weight: Translated: 37001; Mature: 36870

Theoretical pI: Translated: 4.72; Mature: 4.72

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
5.9 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
3.2 %Met     (Mature Protein)
5.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQL
CCCCCCCCCCCCCCCEEECCHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
STLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAW
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
RNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELASAGLDYYNHNLDTSPEFYG
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCCCCCHHHHH
QVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPIN
HHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NLVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGAN
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
SMFYGDQLLTTSNPQSQKDRALFERLGIRSSDADAMSANA
CCEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC
>Mature Secondary Structure 
TQAQTAATVQPDAIPVAAPVSQRWRVADVVALFELPFNDLLFRAQQVHREHFDANAVQL
CCCCCCCCCCCCCCEEECCHHHCHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCHHHH
STLLSIKTGGCEEDCGYCSQSSHHDTGLKAEKLMDVDAVLDAARAAKANGASRFCMGAAW
HHHHHHCCCCCCHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHC
RNPKERHMPALTEMVRGVKELGLETCMTLGMLEDEQAQELASAGLDYYNHNLDTSPEFYG
CCCHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCCHHHHHHHHHCHHHHCCCCCCCHHHHH
QVISTRTYQDRLDTLDRVRDAGINVCCGGIIGMGESRRERAGLISQLANLNPYPDSVPIN
HHHHHHHHHHHHHHHHHHHHCCCCEEECCHHCCCCHHHHHHHHHHHHHCCCCCCCCCCCC
NLVAIEGTPLEGTAPLDPFEFVRTIAVARITMPKAVVRLSAGREQLDDGLQAMCFLAGAN
CEEEEECCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHCCC
SMFYGDQLLTTSNPQSQKDRALFERLGIRSSDADAMSANA
CCEECCEEEECCCCCCHHHHHHHHHHCCCCCCCCHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA