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The map label for this gene is gcvA [H]

Identifier: 78067702

GI number: 78067702

Start: 3391040

End: 3392026

Strand: Direct

Name: gcvA [H]

Synonym: Bcep18194_A6233

Alternate gene names: 78067702

Gene position: 3391040-3392026 (Clockwise)

Preceding gene: 78067693

Following gene: 78067713

Centisome position: 91.8

GC content: 67.78

Gene sequence:

>987_bases
ATGGACCTCCGCCAGCTACCTGCGCTGAACGCGATTCGCGCGTTCGAAGCCGCCGCCCGTCACGAGAATTTCTCGCGCGC
CGCCGACGAGCTGTACGTCACGCACGGCGCGGTCAGCCACCAGGTACGCGCGCTCGAGGAGGAGCTCGGCGTGCAGCTGT
TCACGCGCAACGGCAAGCGGCTGTGCCTGACCGACGCCGGCATGCGCTACGCGCAGCAGATCCGCACCGCGCTGATGGTG
ATCGCCGATGCGACCCGCGACGTGCGCGCGAGCGACCGCGACCGACGGCTCGTGGTGTCGATGCTGTCGTCGTTCGCCGC
GCGCTTCATCACGCCGCGCATCGGCACGTTCATCGAGCGGCATCCGGAAATCGACGTCGAGCTGCAGTCGACCAACTCGC
TCACCGATTTCGCACGCGACGACGTCGACATCGCGATCCGCTTTGGTCAGGGCAACTATCCGGGGCTCCATGTCGAGCCG
CTGTTCGACGAGGTGTTCTTTCCGGCGTGCGCGCCGACGCTCAATGGCGGCAAGCTGCCGCAAACGCCCGCCGATCTCGC
GCACTACAACCTGCTGCGCTCGGACGACGAGCTGTGGCGGCCGTGGTTCGACGCGGCCGGGCTCAAGACGCTGACCGAGC
CGAAACGGGGGATTCTGTACCAGGATTCGTCGAACCTGCTGCTGGCCGCGATCGACGGCCAGGGCATCGCGCTGGTGCGC
CGCTCGCTCGCGGTGCACGACCTGCTCGACGGGCGCATCGTGCGCCTGTTCGACATCGACGGGCCGAGCCCGTGGCACTA
TTTTTTCGTGTGTCCGCCGCCGCTCCTGAACACGCCGCGCGTGCAGGCGTTCAGGACCTGGCTGCTTCAGGAAGTCGCCG
AATACAAGCGGCTGTGCGACGAGCTGGACGCGCGGCGCGCGGCGGGGAAGACCCCCGCCGAGTGCGCGCAGGAAGGAGGC
TGGAAAGCGCTTAGAGTACGACCTTGA

Upstream 100 bases:

>100_bases
TCGACTCCGCCAATAAGTGCTTCGATGGCTACAATCGTAGTCACTTCAAGGCGTCTGACAAAACGACCAATTTTCACGTC
GATGTGAGGAAAATTAGCAA

Downstream 100 bases:

>100_bases
CCATCGGATGGCCGGTCAGTGCGCGGTAGATGTTGTCGAGCTGTTCCTGGCTCGTCGCCCGCACGGTGATCGTGAGGCCC
GTGTAATTACCGCCGCTCGA

Product: DNA-binding transcriptional activator GcvA

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 328; Mature: 328

Protein sequence:

>328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELYVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDIAIRFGQGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLAHYNLLRSDDELWRPWFDAAGLKTLTEPKRGILYQDSSNLLLAAIDGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLLQEVAEYKRLCDELDARRAAGKTPAECAQEGG
WKALRVRP

Sequences:

>Translated_328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELYVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDIAIRFGQGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLAHYNLLRSDDELWRPWFDAAGLKTLTEPKRGILYQDSSNLLLAAIDGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLLQEVAEYKRLCDELDARRAAGKTPAECAQEGG
WKALRVRP
>Mature_328_residues
MDLRQLPALNAIRAFEAAARHENFSRAADELYVTHGAVSHQVRALEEELGVQLFTRNGKRLCLTDAGMRYAQQIRTALMV
IADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIERHPEIDVELQSTNSLTDFARDDVDIAIRFGQGNYPGLHVEP
LFDEVFFPACAPTLNGGKLPQTPADLAHYNLLRSDDELWRPWFDAAGLKTLTEPKRGILYQDSSNLLLAAIDGQGIALVR
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLLQEVAEYKRLCDELDARRAAGKTPAECAQEGG
WKALRVRP

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=292, Percent_Identity=39.3835616438356, Blast_Score=209, Evalue=2e-55,
Organism=Escherichia coli, GI1786448, Length=298, Percent_Identity=32.2147651006711, Blast_Score=134, Evalue=8e-33,
Organism=Escherichia coli, GI1788706, Length=293, Percent_Identity=28.6689419795222, Blast_Score=130, Evalue=1e-31,
Organism=Escherichia coli, GI145693193, Length=303, Percent_Identity=28.7128712871287, Blast_Score=107, Evalue=1e-24,
Organism=Escherichia coli, GI1786401, Length=284, Percent_Identity=28.169014084507, Blast_Score=92, Evalue=7e-20,
Organism=Escherichia coli, GI157672245, Length=158, Percent_Identity=37.3417721518987, Blast_Score=91, Evalue=7e-20,
Organism=Escherichia coli, GI145693105, Length=167, Percent_Identity=26.9461077844311, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787128, Length=139, Percent_Identity=29.4964028776978, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI1787806, Length=261, Percent_Identity=27.9693486590038, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI1787589, Length=271, Percent_Identity=25.0922509225092, Blast_Score=67, Evalue=2e-12,
Organism=Escherichia coli, GI87081978, Length=157, Percent_Identity=28.6624203821656, Blast_Score=66, Evalue=4e-12,
Organism=Escherichia coli, GI1788748, Length=168, Percent_Identity=27.3809523809524, Blast_Score=65, Evalue=5e-12,
Organism=Escherichia coli, GI1789639, Length=257, Percent_Identity=24.124513618677, Blast_Score=64, Evalue=1e-11,
Organism=Escherichia coli, GI1788887, Length=205, Percent_Identity=28.2926829268293, Blast_Score=64, Evalue=2e-11,
Organism=Escherichia coli, GI1787601, Length=171, Percent_Identity=28.0701754385965, Blast_Score=62, Evalue=6e-11,
Organism=Escherichia coli, GI1790262, Length=218, Percent_Identity=26.1467889908257, Blast_Score=61, Evalue=9e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 36903; Mature: 36903

Theoretical pI: Translated: 6.72; Mature: 6.72

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
1.2 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
1.2 %Met     (Mature Protein)
2.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELYVTHGAVSHQVRALEEELGVQLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQIRTALMVIADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
HPEIDVELQSTNSLTDFARDDVDIAIRFGQGNYPGLHVEPLFDEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHCCCCCCCCCCC
QTPADLAHYNLLRSDDELWRPWFDAAGLKTLTEPKRGILYQDSSNLLLAAIDGQGIALVR
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLLQEVAEYKRLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKTPAECAQEGGWKALRVRP
HHHHHHHCCCCHHHHHHCCCCCEEEECC
>Mature Secondary Structure
MDLRQLPALNAIRAFEAAARHENFSRAADELYVTHGAVSHQVRALEEELGVQLFTRNGKR
CCHHHCCHHHHHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHCEEEEECCCCE
LCLTDAGMRYAQQIRTALMVIADATRDVRASDRDRRLVVSMLSSFAARFITPRIGTFIER
EEEEHHHHHHHHHHHHHHHHHHHCCCCCCCCCCHHHHHHHHHHHHHHHHHCHHHHHHHHH
HPEIDVELQSTNSLTDFARDDVDIAIRFGQGNYPGLHVEPLFDEVFFPACAPTLNGGKLP
CCCCEEEECCCCCHHHHHCCCEEEEEEECCCCCCCEEECHHHHHHHHHHCCCCCCCCCCC
QTPADLAHYNLLRSDDELWRPWFDAAGLKTLTEPKRGILYQDSSNLLLAAIDGQGIALVR
CCCHHHHHHHHHCCCHHHHHHHHHHCCCCHHCCCCCCEEEECCCCEEEEEECCCCHHHHH
RSLAVHDLLDGRIVRLFDIDGPSPWHYFFVCPPPLLNTPRVQAFRTWLLQEVAEYKRLCD
HHHHHHHHHCCCEEEEEECCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHHHHHHHHH
ELDARRAAGKTPAECAQEGGWKALRVRP
HHHHHHHCCCCHHHHHHCCCCCEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]