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The map label for this gene is dctD [H]

Identifier: 78067682

GI number: 78067682

Start: 3366966

End: 3368321

Strand: Reverse

Name: dctD [H]

Synonym: Bcep18194_A6213

Alternate gene names: 78067682

Gene position: 3368321-3366966 (Counterclockwise)

Preceding gene: 78067683

Following gene: 78067680

Centisome position: 91.18

GC content: 70.13

Gene sequence:

>1356_bases
ATGGCCAACCGGCTGCAAGTGATCTATATCGAAGACGATGCGCTCGTTCGCCGGGCAAGCGTGCAGAGCCTTCAGCTGGC
GGGGTTCGACGTCGCCGGCTTCGAGTCGGCTGAAGCGGCCGAGAAGGCGATCGTCGCGGATAACGCCGGCGCGATCGTCA
GCGACATCCGGCTGCCCGGCGCGAGCGGGCTCGACGTGCTCGCGCAGTGCCGCGAGCGCGTGCCCGACGTGCCCGTGATC
CTTGTCACGGGGCACGGCGACATTTCGATGGCCGTGCAGGCGATGCGCGACGGCGCGTACGACTTCATCGAAAAGCCGTT
CGCGGCCGAGCGCCTGATCGAGACGGTGCGCCGCGCGCTCGAGCGCCGCGAGCTCGTGCTCGAGAACCACGCGCTGCGGC
GCGAGCTGGCCGGGCAGAACGTCGTCGCGCCGCGCATCATCGGCCGCAGCCCCGCGATCGAACAGGTACGCAAGCTGATC
GCGAACGTCGCGCCGACCGATGCGTCGGTGCTGATCAACGGCGACACCGGCGCCGGCAAGGAGCTGATCGCGCGCAGCCT
GCACGAGCTGTCGCCGCGCCGCGACAAGCCGTTCATCGCGGTGAATTGCGGCGCGTTGCCCGAGCCGATGTTCGAGTCGG
AGATGTTCGGCTACGAGCCCGGCGCGTTCACCGGCGCCGCGAAGCGCCGCGTCGGCAAGCTCGAGTACGCGTCGGGCGGC
ACGCTGTTCCTCGACGAAATCGAAAGCATGCCGCTCGCGCTGCAGGTGAAGCTGCTGCGCGTGCTGCAGGACGGCGTGCT
GGAGCGGCTCGGCTCGAACCAGCCGATCCGCGTGAACTGCCGCGTCGTCGCGGCCGCGAAGGGCGACATGAGCGAGCTCG
TCGCGGCCGGCACGTTCCGGCGCGACCTGCTGTACCGTCTCAACGTCGTGACGATCGCGCTGCCGCCGCTGGCCGAGCGC
CGCGAGGACATCGTGCCGCTGTTCGAGCACTTCATGCTCGATGCGGCCGTGCGTTACGCGCGGCCCGCGCCGGTGCTGAC
CGACCGGCAGCGCGCGAGCCTGATGCAGCGCGACTGGCCCGGCAACGTGCGCGAGCTGCGCAACGCGGCCGACCGCTTCG
TGCTCGGCGTGGCCGACATGCCGCAGGAGACGGGCGCGAGCGGCGACGAGGCCGACAACGAGCAGACGCTGAAGGAGCGC
ATCGAGCAATTCGAGCGCGCGGTGATCGCCGAGGCGCTGAACCAGACGGGTGGCGCGGTTGCCGCGACGGCCGACCGGCT
GCATGTCGGCAAGGCGACGCTGTACGAGAAGATGAAGCGCTACGGGCTGTCGGCGAAAGGCGAAACCGAGCGCTGA

Upstream 100 bases:

>100_bases
GCTAGGCGGCGTCCCGCCACCCGCCCGATCGGCGCACGATCGGTATCAACGACGCCGCGCGCGCTGCCGCCGCGCGGCCC
GTCAGGAGCAAGTGAGTACG

Downstream 100 bases:

>100_bases
AAAGCAAACGGACCGCGGAACCGGGTTGCCCGGTCGTGCGGTCCGTCGTGCGGGGAAGGGCGCGGCGCGCGGCCCTTCGA
GCCGGATCGGGCGGCGCTCA

Product: two component, sigma54 specific, Fis family transcriptional regulator

Products: NADP; 2 glutathione [C]

Alternate protein names: NA

Number of amino acids: Translated: 451; Mature: 450

Protein sequence:

>451_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAAEKAIVADNAGAIVSDIRLPGASGLDVLAQCRERVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYARPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPQETGASGDEADNEQTLKER
IEQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER

Sequences:

>Translated_451_residues
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAAEKAIVADNAGAIVSDIRLPGASGLDVLAQCRERVPDVPVI
LVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLI
ANVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAER
REDIVPLFEHFMLDAAVRYARPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPQETGASGDEADNEQTLKER
IEQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER
>Mature_450_residues
ANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAAEKAIVADNAGAIVSDIRLPGASGLDVLAQCRERVPDVPVIL
VTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRALERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIA
NVAPTDASVLINGDTGAGKELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGGT
LFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFRRDLLYRLNVVTIALPPLAERR
EDIVPLFEHFMLDAAVRYARPAPVLTDRQRASLMQRDWPGNVRELRNAADRFVLGVADMPQETGASGDEADNEQTLKERI
EQFERAVIAEALNQTGGAVAATADRLHVGKATLYEKMKRYGLSAKGETER

Specific function: Member of the two-component regulatory system dctB/dctD involved in the transport of C4-dicarboxylates. When activated by dctB acts in conjunction with sigma-54 to activate the transcription of dctA [H]

COG id: COG2204

COG function: function code T; Response regulator containing CheY-like receiver, AAA-type ATPase, and DNA-binding domains

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 sigma-54 factor interaction domain [H]

Homologues:

Organism=Escherichia coli, GI1790299, Length=470, Percent_Identity=38.5106382978723, Blast_Score=302, Evalue=3e-83,
Organism=Escherichia coli, GI1788550, Length=452, Percent_Identity=38.2743362831858, Blast_Score=298, Evalue=6e-82,
Organism=Escherichia coli, GI1790437, Length=448, Percent_Identity=37.2767857142857, Blast_Score=281, Evalue=7e-77,
Organism=Escherichia coli, GI1788905, Length=446, Percent_Identity=36.9955156950673, Blast_Score=269, Evalue=3e-73,
Organism=Escherichia coli, GI87082117, Length=339, Percent_Identity=42.4778761061947, Blast_Score=243, Evalue=1e-65,
Organism=Escherichia coli, GI1789087, Length=361, Percent_Identity=40.7202216066482, Blast_Score=230, Evalue=1e-61,
Organism=Escherichia coli, GI1789233, Length=320, Percent_Identity=39.0625, Blast_Score=229, Evalue=3e-61,
Organism=Escherichia coli, GI87082152, Length=322, Percent_Identity=42.8571428571429, Blast_Score=226, Evalue=2e-60,
Organism=Escherichia coli, GI87081872, Length=239, Percent_Identity=46.8619246861925, Blast_Score=218, Evalue=4e-58,
Organism=Escherichia coli, GI1786524, Length=286, Percent_Identity=43.7062937062937, Blast_Score=213, Evalue=2e-56,
Organism=Escherichia coli, GI1787583, Length=330, Percent_Identity=38.1818181818182, Blast_Score=203, Evalue=2e-53,
Organism=Escherichia coli, GI87081858, Length=377, Percent_Identity=29.1777188328912, Blast_Score=141, Evalue=8e-35,
Organism=Escherichia coli, GI1789828, Length=236, Percent_Identity=36.0169491525424, Blast_Score=130, Evalue=2e-31,
Organism=Escherichia coli, GI1789402, Length=121, Percent_Identity=33.8842975206612, Blast_Score=67, Evalue=3e-12,
Organism=Escherichia coli, GI1790552, Length=119, Percent_Identity=28.5714285714286, Blast_Score=66, Evalue=5e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003593
- InterPro:   IPR011006
- InterPro:   IPR009057
- InterPro:   IPR002197
- InterPro:   IPR002078
- InterPro:   IPR001789 [H]

Pfam domain/function: PF02954 HTH_8; PF00072 Response_reg; PF00158 Sigma54_activat [H]

EC number: NA

Molecular weight: Translated: 49309; Mature: 49178

Theoretical pI: Translated: 5.63; Mature: 5.63

Prosite motif: PS50110 RESPONSE_REGULATORY ; PS00675 SIGMA54_INTERACT_1 ; PS00676 SIGMA54_INTERACT_2 ; PS00688 SIGMA54_INTERACT_3 ; PS50045 SIGMA54_INTERACT_4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAAEKAIVADNAGAIVSDIRLPG
CCCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCCHHHHCCCCC
ASGLDVLAQCRERVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYARPAPVLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVADMPQETGASGDEADNEQTLKERIEQFERAVIAEALNQTGGAV
CCHHHHHHHHHHEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
AATADRLHVGKATLYEKMKRYGLSAKGETER
EEEHHHHHCCHHHHHHHHHHHCCCCCCCCCC
>Mature Secondary Structure 
ANRLQVIYIEDDALVRRASVQSLQLAGFDVAGFESAEAAEKAIVADNAGAIVSDIRLPG
CCCEEEEEECCHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHEECCCCCHHHHCCCCC
ASGLDVLAQCRERVPDVPVILVTGHGDISMAVQAMRDGAYDFIEKPFAAERLIETVRRAL
CCCHHHHHHHHHHCCCCCEEEEECCCCHHHHHHHHHCCCHHHHHCCHHHHHHHHHHHHHH
ERRELVLENHALRRELAGQNVVAPRIIGRSPAIEQVRKLIANVAPTDASVLINGDTGAGK
HHHHHHHHHHHHHHHHCCCCCCCCHHCCCCHHHHHHHHHHHHCCCCCCEEEEECCCCCCH
ELIARSLHELSPRRDKPFIAVNCGALPEPMFESEMFGYEPGAFTGAAKRRVGKLEYASGG
HHHHHHHHHCCCCCCCCEEEEECCCCCCCHHHHHHCCCCCCCCCCHHHHHHCCEEECCCC
TLFLDEIESMPLALQVKLLRVLQDGVLERLGSNQPIRVNCRVVAAAKGDMSELVAAGTFR
EEEHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEEEECCCCHHHHHHHHHHH
RDLLYRLNVVTIALPPLAERREDIVPLFEHFMLDAAVRYARPAPVLTDRQRASLMQRDWP
HHHHHHHEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHCCCC
GNVRELRNAADRFVLGVADMPQETGASGDEADNEQTLKERIEQFERAVIAEALNQTGGAV
CCHHHHHHHHHHEEEECCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHCCCCEE
AATADRLHVGKATLYEKMKRYGLSAKGETER
EEEHHHHHCCHHHHHHHHHHHCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NADPH2; oxidized glutathione [C]

Specific reaction: NADPH2 + oxidized glutathione = NADP + 2 glutathione [C]

General reaction: Oxidoreductases; Acting on NADH or NADPH; With a disulfide as acceptor [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 2134335; 2793824; 11481431 [H]