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The map label for this gene is glpK

Identifier: 78067475

GI number: 78067475

Start: 3140231

End: 3141748

Strand: Direct

Name: glpK

Synonym: Bcep18194_A6006

Alternate gene names: 78067475

Gene position: 3140231-3141748 (Clockwise)

Preceding gene: 78067474

Following gene: 78067476

Centisome position: 85.01

GC content: 65.68

Gene sequence:

>1518_bases
ATGGAGATGAGAGACATGCAGGATCAGTACATCCTCGCGCTTGACCAGGGCACCACGAGTTCCCGCGCGATGCTGTTCGA
TCGCCAGGGCAATATCGTTTCGATCGCCCAGAAGGAATTCGAGCAGATCTACCCGCAACCCGGCTGGGTCGAGCACGATC
CGCAGGAAATCTGGTCGACGCAAGCCGGCGTCGCCGCCGAAGCCGTCACGCGCACGGGCCTGAACGGCACGTCGATCGCC
GCGATCGGCATCACCAACCAGCGTGAAACCACGATCGTCTGGGATCGCGAGACGGGCCAGCCCGTCTACAACGCGATCGT
CTGGCAGGATCGCCGCACGGCCGACTTCTGCGATTCGCTGAAAGCACAGGGCTTCGAGGCGAAGGTGCGTGCGAAGACCG
GCCTGCCGATCGATTCGTACTTCTCAGGGACCAAGATCCGCTGGATTCTCGACAACGTGCCGGGCGCACGCGAGAAGGCG
AAGCAGGGCAAGCTCGCGTTCGGCACCGTCGACAGCTGGCTCGTGTGGAACTTCACGAAGCACGAACTGCACGTGACCGA
CGTGACGAACGCGTCGCGTACGATGCTGTTCAACATCCACACGCGCGAGTGGGACAACGAACTGCTCGACATGCTCGACA
TCCCGCGCAGCATGCTGCCGGAAGTGAAGGCGTCGTCGGAAATCTACGGCCATACGAAGACCACCGTGTTCGCGTCGAAG
ATCCCGCTCGCGGGTATCGCGGGCGACCAGCAGGCGGCGCTGTTCGGCCAGATGTGCACGCAGTCCGGCATGGTGAAGAA
CACCTACGGTACCGGCTGCTTCCTGATGATGAACACCGGCGACAAGCCGATCGAATCGAAGAACAACCTCGTCACGACGA
TCGCGTGGCAGATCGGCGACAACGTGCAGTACGCGCTGGAAGGCAGCATCTTCATCGCGGGCGCGGTGGTGCAGTGGCTG
CGCGACGGCGTCGGCATCATCAAGTCGGCCGCCGAGATCGAAGCGCTCGCCGCAAGCGTGCCGCACACCGACGGCGTCTA
CCTGGTGCCCGCCTTCGCCGGCCTCGGCGCACCGCACTGGAACGCGCGGGCGCGCGGCTCGGTGTTCGGCGTCACGCGCG
GCACAACCTCCGCGCACCTCGCACGCGCGGCGCTCGATTCGATCGCGTACCAGTCGCTCGACGTGCTGGCCGCGATGGAA
GCCGATTCGGGCATCAGCATCGGCGAGCTGCGCGTCGACGGCGGCGCGAGCGCGAACGACCTGCTGATGCAGTTCCAGGC
GGACCTGCTCGGCGTTGACGCGGTGCGTCCGCAGATCACCGAGACGACCGCGCTCGGCGCGGCCTACCTCGCGGGCCTCG
CGATCGGCTACTGGAAGAGCCTCGACGACGTGCGCGACCAGTGGCAGCTCGATCGCCGCTTCTCGCCGTCGATGCCGAAG
GAGCACGTCGAACGCTGCATGTCGGGCTGGCAGCGTGCGGTGCGTGCCGCGAAGGCATGGGCCGACGACGCCCAGTAA

Upstream 100 bases:

>100_bases
CAGGCCTGATCCGAATGCAGTTGCAGTTGCCGTACCGACGTTGACTCACCGACGCGCCGCCCAACATGCGGCGCGCATCA
CAACTAAGCCAAATCCACGG

Downstream 100 bases:

>100_bases
TCCTCACACACTTAAACGAAATACAACAACACCGGCGGACCGCGCCACGCGCGAGTCCGCGATGACATACGAGAGACAAC
CATGTCACCTTATATTGCAG

Product: glycerol kinase

Products: NA

Alternate protein names: ATP:glycerol 3-phosphotransferase; Glycerokinase; GK [H]

Number of amino acids: Translated: 505; Mature: 505

Protein sequence:

>505_residues
MEMRDMQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIA
AIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKAQGFEAKVRAKTGLPIDSYFSGTKIRWILDNVPGAREKA
KQGKLAFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTREWDNELLDMLDIPRSMLPEVKASSEIYGHTKTTVFASK
IPLAGIAGDQQAALFGQMCTQSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDNVQYALEGSIFIAGAVVQWL
RDGVGIIKSAAEIEALAASVPHTDGVYLVPAFAGLGAPHWNARARGSVFGVTRGTTSAHLARAALDSIAYQSLDVLAAME
ADSGISIGELRVDGGASANDLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKSLDDVRDQWQLDRRFSPSMPK
EHVERCMSGWQRAVRAAKAWADDAQ

Sequences:

>Translated_505_residues
MEMRDMQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIA
AIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKAQGFEAKVRAKTGLPIDSYFSGTKIRWILDNVPGAREKA
KQGKLAFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTREWDNELLDMLDIPRSMLPEVKASSEIYGHTKTTVFASK
IPLAGIAGDQQAALFGQMCTQSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDNVQYALEGSIFIAGAVVQWL
RDGVGIIKSAAEIEALAASVPHTDGVYLVPAFAGLGAPHWNARARGSVFGVTRGTTSAHLARAALDSIAYQSLDVLAAME
ADSGISIGELRVDGGASANDLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKSLDDVRDQWQLDRRFSPSMPK
EHVERCMSGWQRAVRAAKAWADDAQ
>Mature_505_residues
MEMRDMQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWSTQAGVAAEAVTRTGLNGTSIA
AIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSLKAQGFEAKVRAKTGLPIDSYFSGTKIRWILDNVPGAREKA
KQGKLAFGTVDSWLVWNFTKHELHVTDVTNASRTMLFNIHTREWDNELLDMLDIPRSMLPEVKASSEIYGHTKTTVFASK
IPLAGIAGDQQAALFGQMCTQSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGDNVQYALEGSIFIAGAVVQWL
RDGVGIIKSAAEIEALAASVPHTDGVYLVPAFAGLGAPHWNARARGSVFGVTRGTTSAHLARAALDSIAYQSLDVLAAME
ADSGISIGELRVDGGASANDLLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKSLDDVRDQWQLDRRFSPSMPK
EHVERCMSGWQRAVRAAKAWADDAQ

Specific function: Key enzyme in the regulation of glycerol uptake and metabolism [H]

COG id: COG0554

COG function: function code C; Glycerol kinase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the FGGY kinase family [H]

Homologues:

Organism=Homo sapiens, GI189409120, Length=502, Percent_Identity=45.6175298804781, Blast_Score=444, Evalue=1e-124,
Organism=Homo sapiens, GI4504007, Length=502, Percent_Identity=45.6175298804781, Blast_Score=443, Evalue=1e-124,
Organism=Homo sapiens, GI42794763, Length=507, Percent_Identity=45.1676528599605, Blast_Score=441, Evalue=1e-124,
Organism=Homo sapiens, GI41393575, Length=502, Percent_Identity=44.0239043824701, Blast_Score=433, Evalue=1e-121,
Organism=Homo sapiens, GI88196792, Length=509, Percent_Identity=32.6129666011788, Blast_Score=311, Evalue=1e-84,
Organism=Escherichia coli, GI1790361, Length=497, Percent_Identity=58.148893360161, Blast_Score=638, Evalue=0.0,
Organism=Escherichia coli, GI1790005, Length=499, Percent_Identity=25.8517034068136, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI1789168, Length=493, Percent_Identity=25.5578093306288, Blast_Score=110, Evalue=2e-25,
Organism=Escherichia coli, GI1789987, Length=474, Percent_Identity=24.2616033755274, Blast_Score=109, Evalue=5e-25,
Organism=Escherichia coli, GI1787789, Length=498, Percent_Identity=25.7028112449799, Blast_Score=98, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17535599, Length=500, Percent_Identity=43.4, Blast_Score=417, Evalue=1e-117,
Organism=Caenorhabditis elegans, GI71986140, Length=525, Percent_Identity=24.1904761904762, Blast_Score=173, Evalue=2e-43,
Organism=Caenorhabditis elegans, GI71986147, Length=303, Percent_Identity=29.3729372937294, Blast_Score=122, Evalue=4e-28,
Organism=Saccharomyces cerevisiae, GI6321755, Length=555, Percent_Identity=35.6756756756757, Blast_Score=315, Evalue=1e-86,
Organism=Drosophila melanogaster, GI17864214, Length=505, Percent_Identity=42.7722772277228, Blast_Score=408, Evalue=1e-114,
Organism=Drosophila melanogaster, GI24655555, Length=473, Percent_Identity=42.2832980972516, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655560, Length=473, Percent_Identity=42.2832980972516, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655570, Length=473, Percent_Identity=42.2832980972516, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655574, Length=473, Percent_Identity=42.2832980972516, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24655565, Length=473, Percent_Identity=42.2832980972516, Blast_Score=394, Evalue=1e-110,
Organism=Drosophila melanogaster, GI24654573, Length=445, Percent_Identity=43.3707865168539, Blast_Score=377, Evalue=1e-104,
Organism=Drosophila melanogaster, GI19922058, Length=527, Percent_Identity=33.0170777988615, Blast_Score=292, Evalue=4e-79,
Organism=Drosophila melanogaster, GI24652871, Length=494, Percent_Identity=33.8056680161943, Blast_Score=278, Evalue=8e-75,
Organism=Drosophila melanogaster, GI24656367, Length=514, Percent_Identity=31.1284046692607, Blast_Score=249, Evalue=3e-66,
Organism=Drosophila melanogaster, GI21357867, Length=526, Percent_Identity=30.4182509505703, Blast_Score=247, Evalue=2e-65,
Organism=Drosophila melanogaster, GI24656372, Length=363, Percent_Identity=31.6804407713499, Blast_Score=184, Evalue=1e-46,
Organism=Drosophila melanogaster, GI45552909, Length=303, Percent_Identity=29.3729372937294, Blast_Score=134, Evalue=1e-31,

Paralogues:

None

Copy number: 2,500 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000577
- InterPro:   IPR018485
- InterPro:   IPR018483
- InterPro:   IPR018484
- InterPro:   IPR005999 [H]

Pfam domain/function: PF02782 FGGY_C; PF00370 FGGY_N [H]

EC number: =2.7.1.30 [H]

Molecular weight: Translated: 55298; Mature: 55298

Theoretical pI: Translated: 5.20; Mature: 5.20

Prosite motif: PS00933 FGGY_KINASES_1 ; PS00445 FGGY_KINASES_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.0 %Met     (Translated Protein)
3.8 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MEMRDMQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWST
CCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCC
QAGVAAEAVTRTGLNGTSIAAIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSL
CCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHH
KAQGFEAKVRAKTGLPIDSYFSGTKIRWILDNVPGAREKAKQGKLAFGTVDSWLVWNFTK
HCCCCEEEEEECCCCCHHHCCCCCEEEEEECCCCCHHHHHHCCCEEEECCCCEEEEEEEC
HELHVTDVTNASRTMLFNIHTREWDNELLDMLDIPRSMLPEVKASSEIYGHTKTTVFASK
CEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHCCHHHCCCHHCCCHHCCCCEEEEEEEC
IPLAGIAGDQQAALFGQMCTQSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGD
CCEEECCCCHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCEEEEEEEEECC
NVQYALEGSIFIAGAVVQWLRDGVGIIKSAAEIEALAASVPHTDGVYLVPAFAGLGAPHW
CEEEEEECCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCC
NARARGSVFGVTRGTTSAHLARAALDSIAYQSLDVLAAMEADSGISIGELRVDGGASAND
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHH
LLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKSLDDVRDQWQLDRRFSPSMPK
HHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EHVERCMSGWQRAVRAAKAWADDAQ
HHHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure
MEMRDMQDQYILALDQGTTSSRAMLFDRQGNIVSIAQKEFEQIYPQPGWVEHDPQEIWST
CCCCCCCCCEEEEEECCCCCCEEEEEECCCCEEEHHHHHHHHHCCCCCCCCCCHHHHHCC
QAGVAAEAVTRTGLNGTSIAAIGITNQRETTIVWDRETGQPVYNAIVWQDRRTADFCDSL
CCCHHHHHHHHCCCCCCEEEEEEECCCCCEEEEEECCCCCHHHHHEEECCCCCHHHHHHH
KAQGFEAKVRAKTGLPIDSYFSGTKIRWILDNVPGAREKAKQGKLAFGTVDSWLVWNFTK
HCCCCEEEEEECCCCCHHHCCCCCEEEEEECCCCCHHHHHHCCCEEEECCCCEEEEEEEC
HELHVTDVTNASRTMLFNIHTREWDNELLDMLDIPRSMLPEVKASSEIYGHTKTTVFASK
CEEEEEECCCCCEEEEEEEECCHHHHHHHHHHHCCHHHCCCHHCCCHHCCCCEEEEEEEC
IPLAGIAGDQQAALFGQMCTQSGMVKNTYGTGCFLMMNTGDKPIESKNNLVTTIAWQIGD
CCEEECCCCHHHHHHHHHHHCCCCEEECCCCEEEEEECCCCCCCCCCCCEEEEEEEEECC
NVQYALEGSIFIAGAVVQWLRDGVGIIKSAAEIEALAASVPHTDGVYLVPAFAGLGAPHW
CEEEEEECCEEEHHHHHHHHHCCHHHHHHHHHHHHHHHCCCCCCCEEEEEEHHCCCCCCC
NARARGSVFGVTRGTTSAHLARAALDSIAYQSLDVLAAMEADSGISIGELRVDGGASAND
CCCCCCCEEEEECCCCHHHHHHHHHHHHHHHHHHHHEEECCCCCCEEEEEEECCCCCHHH
LLMQFQADLLGVDAVRPQITETTALGAAYLAGLAIGYWKSLDDVRDQWQLDRRFSPSMPK
HHHHHHHHHHCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCH
EHVERCMSGWQRAVRAAKAWADDAQ
HHHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA