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The map label for this gene is proP [H]

Identifier: 78067451

GI number: 78067451

Start: 3112698

End: 3114356

Strand: Direct

Name: proP [H]

Synonym: Bcep18194_A5982

Alternate gene names: 78067451

Gene position: 3112698-3114356 (Clockwise)

Preceding gene: 78067450

Following gene: 78067452

Centisome position: 84.26

GC content: 65.22

Gene sequence:

>1659_bases
ATGGCAACGTTAGGCGGGCAAATTTCGCACGCGCCGATGACGGGCGAAGAGAAGAAGGTGATCTTCGCGTCGTCGCTCGG
CACCGTGTTCGAGTGGTACGACTTTTACCTGGCCGGCTCGCTCGCGGCCTACATCAGCAAGAGTTTCTTCTCCGGTGTCA
ATCCAACTGCCGCCTTCATCTTCACGCTGCTCGGCTTCGCGGCCGGCTTCGCGGTGCGGCCGTTCGGCGCGATCGTGTTC
GGCCGGCTCGGCGACCTCGTCGGACGCAAGCACACGTTCCTCGTAACGATCGTGATCATGGGCCTCTCGACGTTCGTGGT
CGGCTTCCTGCCCGGCTACGCATCGATCGGCATCGCCGCGCCCGTGATCTTCATCGCGATGCGGCTGTTGCAGGGCCTCG
CGCTCGGCGGCGAGTACGGCGGCGCGGCGACCTACGTCGCCGAGCATGCGCCGGCCAACCGTCGCGGCTTCTACACCGCG
TGGATCCAGACGACCGCCACGCTCGGCCTGTTCCTGTCGCTGCTCGTGATCCTCGGCATCCGCACGCTCATCGGCGAGGA
AGCGTTCGGCAACTGGGGCTGGCGCGTGCCGTTCGTCGCGTCGATCCTGCTGCTCGCGGTGTCGGTATGGATCCGGATGC
AACTGAACGAATCGCCGGTGTTCCTGCGCATCAAGGCGGAAGGCAAGACGTCGAAGGCGCCGCTGACCGAAGCGTTCGGC
CAGTGGAAGAACCTGAAGATCGTGATCCTCGCGCTCGTCGGCCTAACCGCCGGCCAGGCTGTCGTGTGGTACACGGGCCA
GTTCTACGCGCTGTTCTTCCTCACGCAGACGCTGAAGGTGGACGGGGCAAGCGCGAACATCCTGATCGCGATCGCGCTCC
TGATCGGCACGCCGTTCTTCCTGTTCTTCGGGTCGCTGTCGGACAAGATCGGCCGCAAGCCGATCATCCTCGCGGGCTGC
CTGATCGCGGCGCTGACCTACTTCCCGCTGTTCAAGGCGCTCACGCACTACGCGAACCCGCAGCTCGAACTCGCGACGCA
GAAGGCGCCGATCACGGTCATCGCCGATCCGGCCGCCTGCTCGTTCCAGTTCAACCCGGTGGGCACGTCGAAGTTCACGA
ACTCGTGCGACGTCGCGAAGAGCGCGCTCGCGAAGGCCGGCCTGAACTACGAGAACGTCGCGGCACCGGCTGGCGCGACA
GCCCAGATCAAGGTCGGCGACACGGTGGTCCCCGCCTATGAAGGCAAGGCGGCCGACGCGAAGGCGCAGGGTGCGGCGTT
CGACAAGACGCTCGCGTCGACGCTGAAGGCGGCCGGCTACCCGGCGAAGGCGGACCCGGCGCAGCTCAACTGGCCGATGA
CGATCGTGATCCTGACGATCCTCGTGATCTACGTGACGATGGTCTACGGCCCGATCGCGGCGATGCTGGTCGAGATGTTC
CCGACGCGGATCCGCTACACGTCGATGTCGCTGCCCTATCACATCGGCAACGGCTGGTTCGGCGGCTTCCTGCCGGCCAC
CGCGTTCGCGATCGTCGCGGCGAAGGGCGACATCTACTCGGGCCTCTGGTATCCGATCGTGATCGCGCTGGCCACGTTCG
TGATCGGGCTGCTGTTCGTCAAGGAGACGAAGGACTCGAACATCTACGCTCAGGATTGA

Upstream 100 bases:

>100_bases
CGCGAGTCAGTCGGCCGTAAGTTTCCGTACCAATAATCCTCGACAGGCCCGCTCACCCAAGGCGGCCGCACATCTATATC
AAGGGACTTGGAGACGACTC

Downstream 100 bases:

>100_bases
GGGGGATGTGCGGACGGTTGCCGGCGGTGCACGGTGATCGTCCGCAACAAGGTCGGCACGGGCCGCCGGCGCCTTTTTCA
CGGATGATGAAAAAAGGTGT

Product: major facilitator transporter

Products: betaine [Cytoplasm]; Proton [Cytoplasm]; L-proline [Cytoplasm] [C]

Alternate protein names: Proline porter II; PPII [H]

Number of amino acids: Translated: 552; Mature: 551

Protein sequence:

>552_residues
MATLGGQISHAPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGLSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGIRTLIGEEAFGNWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPITVIADPAACSFQFNPVGTSKFTNSCDVAKSALAKAGLNYENVAAPAGAT
AQIKVGDTVVPAYEGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD

Sequences:

>Translated_552_residues
MATLGGQISHAPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVF
GRLGDLVGRKHTFLVTIVIMGLSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTA
WIQTTATLGLFLSLLVILGIRTLIGEEAFGNWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKAEGKTSKAPLTEAFG
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGC
LIAALTYFPLFKALTHYANPQLELATQKAPITVIADPAACSFQFNPVGTSKFTNSCDVAKSALAKAGLNYENVAAPAGAT
AQIKVGDTVVPAYEGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD
>Mature_551_residues
ATLGGQISHAPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFIFTLLGFAAGFAVRPFGAIVFG
RLGDLVGRKHTFLVTIVIMGLSTFVVGFLPGYASIGIAAPVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAW
IQTTATLGLFLSLLVILGIRTLIGEEAFGNWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKAEGKTSKAPLTEAFGQ
WKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFFLFFGSLSDKIGRKPIILAGCL
IAALTYFPLFKALTHYANPQLELATQKAPITVIADPAACSFQFNPVGTSKFTNSCDVAKSALAKAGLNYENVAAPAGATA
QIKVGDTVVPAYEGKAADAKAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMFP
TRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALATFVIGLLFVKETKDSNIYAQD

Specific function: Proton symporter that senses osmotic shifts and responds by importing osmolytes such as proline, glycine betaine, stachydrine, pipecolic acid, ectoine and taurine. It is both an osmosensor and an osmoregulator which is available to participate early in th

COG id: COG0477

COG function: function code GEPR; Permeases of the major facilitator superfamily

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1790550, Length=328, Percent_Identity=35.3658536585366, Blast_Score=198, Evalue=7e-52,
Organism=Escherichia coli, GI1788292, Length=327, Percent_Identity=34.5565749235474, Blast_Score=185, Evalue=5e-48,
Organism=Escherichia coli, GI1788942, Length=323, Percent_Identity=34.984520123839, Blast_Score=168, Evalue=9e-43,
Organism=Escherichia coli, GI1789941, Length=220, Percent_Identity=42.7272727272727, Blast_Score=151, Evalue=9e-38,
Organism=Escherichia coli, GI87082231, Length=213, Percent_Identity=27.6995305164319, Blast_Score=74, Evalue=2e-14,
Organism=Escherichia coli, GI87082404, Length=317, Percent_Identity=25.2365930599369, Blast_Score=68, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR015041
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF08946 Osmo_CC; PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 58950; Mature: 58819

Theoretical pI: Translated: 9.65; Mature: 9.65

Prosite motif: PS50850 MFS ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
1.8 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATLGGQISHAPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGLSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTLIGEEAFGNWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPITVIADPAAC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTNSCDVAKSALAKAGLNYENVAAPAGATAQIKVGDTVVPAYEGKAADA
EEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCC
KAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALATFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
EECCCCCCEECC
>Mature Secondary Structure 
ATLGGQISHAPMTGEEKKVIFASSLGTVFEWYDFYLAGSLAAYISKSFFSGVNPTAAFI
CCCCCCCCCCCCCCCCCEEEEECHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHH
FTLLGFAAGFAVRPFGAIVFGRLGDLVGRKHTFLVTIVIMGLSTFVVGFLPGYASIGIAA
HHHHHHHHCCHHCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHCCHHHHHHHH
PVIFIAMRLLQGLALGGEYGGAATYVAEHAPANRRGFYTAWIQTTATLGLFLSLLVILGI
HHHHHHHHHHHHHHCCCCCCCHHHHHHHCCCCCCCCCEEEHHHHHHHHHHHHHHHHHHHH
RTLIGEEAFGNWGWRVPFVASILLLAVSVWIRMQLNESPVFLRIKAEGKTSKAPLTEAFG
HHHHCCHHCCCCCCCHHHHHHHHHHHHHHHHEEEECCCCEEEEEEECCCCCCCCHHHHHC
QWKNLKIVILALVGLTAGQAVVWYTGQFYALFFLTQTLKVDGASANILIAIALLIGTPFF
CCCCCHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHEECCCCCHHHHHHHHHHCCHHH
LFFGSLSDKIGRKPIILAGCLIAALTYFPLFKALTHYANPQLELATQKAPITVIADPAAC
HHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCEEEEECCCEE
SFQFNPVGTSKFTNSCDVAKSALAKAGLNYENVAAPAGATAQIKVGDTVVPAYEGKAADA
EEEECCCCCCCCCCHHHHHHHHHHHCCCCHHHCCCCCCCEEEEEECCEEECCCCCCCCCC
KAQGAAFDKTLASTLKAAGYPAKADPAQLNWPMTIVILTILVIYVTMVYGPIAAMLVEMF
CCCCCHHHHHHHHHHHHCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
PTRIRYTSMSLPYHIGNGWFGGFLPATAFAIVAAKGDIYSGLWYPIVIALATFVIGLLFV
HHHEEEEEECCCEEECCCCCCHHHHHHHHHHHEECCCHHCCHHHHHHHHHHHHHHHHHHH
KETKDSNIYAQD
EECCCCCCEECC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: betaine [Periplasm]; Proton [Periplasm]; L-proline [Periplasm] [C]

Specific reaction: Proton [Periplasm] + betaine [Periplasm] = Proton [Cytoplasm] + betaine [Cytoplasm] Proton [Periplasm] + L-proline [Periplasm] = Proton [Cytoplasm] + L-proline [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]