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The map label for this gene is estB [H]

Identifier: 78067389

GI number: 78067389

Start: 3043561

End: 3044757

Strand: Reverse

Name: estB [H]

Synonym: Bcep18194_A5920

Alternate gene names: 78067389

Gene position: 3044757-3043561 (Counterclockwise)

Preceding gene: 78067391

Following gene: 78067388

Centisome position: 82.42

GC content: 72.93

Gene sequence:

>1197_bases
ATGTCATCCCTGCCTGTCTCCGCTGTGCGCGCGGAACCGGACGCCGCGCTGCGCCACCGGCTCGACGATGCGCTCGATCG
CGCGCTTGCCGACGCGCGCATGGTCGGTGCGGTCGTGCTGGTCGCGCGGGACGGCGAGCTGCGCTATGCGCGCGCCGTCG
GGCTTGCCGATCGCGAAGCCGGCACGCCGATGCGGGAAGACGCGCTGTTCCGTCTGTCGTCGGTCACGAAGCCGGTCGTG
GCGGCCGCCGCGATGCGGCTGGTCGCGGCAGGCAGCATCGCGCTCGACGATCCGGTCACGCGCTGGTTGCCCGGGTTCCG
TCCGGCGTTGCCAGACGGCACGCCTGCCGTCATCACGTTGCGTCATCTGCTATCGCATACGGCCGGCCTCGGCTACCGCT
TCCTCGAAGCGGACGGCGACGGCCCGTATGCGCGCGCCAGCGTGTCCGACGGGATGGATCGCTCGGGTCTGTCGCTCGCC
GAGAACCTGCGGCGCATCGCCGGCGTGCCGCTGCAGTTCGCGCCGGGCACGTCGTGGGGCTATTCGCTCGCGATCGACGT
GGTCGGCGCGCTGATCGAGGCGGTCGACGGGCGGCCGCTCGCCGACGCGGTGGCCGCACTCGTCACGACACCGCTCGGGA
TGACCGACACCGCGTTCGTCGCGCCCGACGCCACGCGCTTCGCGACGCCGTACGTGAGCACGCCGGGCGCCCCGCGCCGG
ATGGCGGACGTCGATCTCGTGCCGGTGTTCGACGGCACGATCGGCATCCGCTTCGAGCCGGCGCGCGCGTTCGATGTCGA
TGCGTGGCCGTCGGGCGGCGCGGGGATGGTCGGTACCGCGCGCGACTGCGTGACGCTGCTCGACACGCTGCGCACGGGCC
GTGACGGTTGGCTTGGGCGCGCATGGACCGACGAGATGGCGCGCGCGCAGCCGGGTGCGCACGACCTGCGGGATGCGCCG
GGTTTCGGCTTCGGGCTCGGCTTTTCGGTGCTGCGCGACCCGGTGGCCGCGCAGTCGCCGGAGTCGGTCGGCACCTGGCG
CGGCGGCGGCGCATACGGGCATACGTGGTTCGTCGACCGCGCGGCCGGGCTGACGGTCGTCGCGCTGTCCAACACGCTGT
ACGAGGGCCTCAATGGGCGGATCGTGACCTGTGTGCGCGATGCGGTCTACGGCGTCGGAACCGGGAGCGCGGCATGA

Upstream 100 bases:

>100_bases
TGCGGACGCCGGCGTCCGGATTGTCCGGAACTCTACCCGGGTTTTTCTTCTTGCGTCGGCTCCCTACGATGAGCGCTCGT
TCACTGTATGAGGTCACGCC

Downstream 100 bases:

>100_bases
GCGATTGCACGTCGATCGATGACGAACTGGCCGTGCGACCGGTGCAGGCCGGCACGCGCCTGCCGGTCGCGAACCTGCTG
GCGCTCGCGACGGCCGGTTT

Product: Beta-lactamase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 398; Mature: 397

Protein sequence:

>398_residues
MSSLPVSAVRAEPDAALRHRLDDALDRALADARMVGAVVLVARDGELRYARAVGLADREAGTPMREDALFRLSSVTKPVV
AAAAMRLVAAGSIALDDPVTRWLPGFRPALPDGTPAVITLRHLLSHTAGLGYRFLEADGDGPYARASVSDGMDRSGLSLA
ENLRRIAGVPLQFAPGTSWGYSLAIDVVGALIEAVDGRPLADAVAALVTTPLGMTDTAFVAPDATRFATPYVSTPGAPRR
MADVDLVPVFDGTIGIRFEPARAFDVDAWPSGGAGMVGTARDCVTLLDTLRTGRDGWLGRAWTDEMARAQPGAHDLRDAP
GFGFGLGFSVLRDPVAAQSPESVGTWRGGGAYGHTWFVDRAAGLTVVALSNTLYEGLNGRIVTCVRDAVYGVGTGSAA

Sequences:

>Translated_398_residues
MSSLPVSAVRAEPDAALRHRLDDALDRALADARMVGAVVLVARDGELRYARAVGLADREAGTPMREDALFRLSSVTKPVV
AAAAMRLVAAGSIALDDPVTRWLPGFRPALPDGTPAVITLRHLLSHTAGLGYRFLEADGDGPYARASVSDGMDRSGLSLA
ENLRRIAGVPLQFAPGTSWGYSLAIDVVGALIEAVDGRPLADAVAALVTTPLGMTDTAFVAPDATRFATPYVSTPGAPRR
MADVDLVPVFDGTIGIRFEPARAFDVDAWPSGGAGMVGTARDCVTLLDTLRTGRDGWLGRAWTDEMARAQPGAHDLRDAP
GFGFGLGFSVLRDPVAAQSPESVGTWRGGGAYGHTWFVDRAAGLTVVALSNTLYEGLNGRIVTCVRDAVYGVGTGSAA
>Mature_397_residues
SSLPVSAVRAEPDAALRHRLDDALDRALADARMVGAVVLVARDGELRYARAVGLADREAGTPMREDALFRLSSVTKPVVA
AAAMRLVAAGSIALDDPVTRWLPGFRPALPDGTPAVITLRHLLSHTAGLGYRFLEADGDGPYARASVSDGMDRSGLSLAE
NLRRIAGVPLQFAPGTSWGYSLAIDVVGALIEAVDGRPLADAVAALVTTPLGMTDTAFVAPDATRFATPYVSTPGAPRRM
ADVDLVPVFDGTIGIRFEPARAFDVDAWPSGGAGMVGTARDCVTLLDTLRTGRDGWLGRAWTDEMARAQPGAHDLRDAPG
FGFGLGFSVLRDPVAAQSPESVGTWRGGGAYGHTWFVDRAAGLTVVALSNTLYEGLNGRIVTCVRDAVYGVGTGSAA

Specific function: Acts on short-chain (C4-C6) fatty acid esters and triglycerides, including tertiary alcohol esters. Activity on p- nitrophenyl esters is generally higher than on o-nitrophenyl esters. Lacks beta-lactamase activity; it hydrolyzes the ester bond of cephalos

COG id: COG1680

COG function: function code V; Beta-lactamase class C and other penicillin binding proteins

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-A beta-lactamase family [H]

Homologues:

Organism=Escherichia coli, GI87082101, Length=374, Percent_Identity=26.2032085561497, Blast_Score=81, Evalue=2e-16,
Organism=Escherichia coli, GI1786574, Length=224, Percent_Identity=29.4642857142857, Blast_Score=67, Evalue=2e-12,
Organism=Caenorhabditis elegans, GI17550934, Length=208, Percent_Identity=29.3269230769231, Blast_Score=79, Evalue=3e-15,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR012338
- InterPro:   IPR001466
- InterPro:   IPR000871 [H]

Pfam domain/function: PF00144 Beta-lactamase [H]

EC number: NA

Molecular weight: Translated: 41623; Mature: 41491

Theoretical pI: Translated: 5.24; Mature: 5.24

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.5 %Cys     (Translated Protein)
2.3 %Met     (Translated Protein)
2.8 %Cys+Met (Translated Protein)
0.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
2.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSSLPVSAVRAEPDAALRHRLDDALDRALADARMVGAVVLVARDGELRYARAVGLADREA
CCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEHHHHCCCCCCC
GTPMREDALFRLSSVTKPVVAAAAMRLVAAGSIALDDPVTRWLPGFRPALPDGTPAVITL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCCCHHHHHH
RHLLSHTAGLGYRFLEADGDGPYARASVSDGMDRSGLSLAENLRRIAGVPLQFAPGTSWG
HHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCC
YSLAIDVVGALIEAVDGRPLADAVAALVTTPLGMTDTAFVAPDATRFATPYVSTPGAPRR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCCCCCCCCC
MADVDLVPVFDGTIGIRFEPARAFDVDAWPSGGAGMVGTARDCVTLLDTLRTGRDGWLGR
CCCCEEEEEECCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
AWTDEMARAQPGAHDLRDAPGFGFGLGFSVLRDPVAAQSPESVGTWRGGGAYGHTWFVDR
HHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEEC
AAGLTVVALSNTLYEGLNGRIVTCVRDAVYGVGTGSAA
CCCEEEEEEHHHHHHCCCCCEEEEEHHHHHCCCCCCCC
>Mature Secondary Structure 
SSLPVSAVRAEPDAALRHRLDDALDRALADARMVGAVVLVARDGELRYARAVGLADREA
CCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHEEEEEEEEECCCEEEHHHHCCCCCCC
GTPMREDALFRLSSVTKPVVAAAAMRLVAAGSIALDDPVTRWLPGFRPALPDGTPAVITL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEECCCHHHHCCCCCCCCCCCCHHHHHH
RHLLSHTAGLGYRFLEADGDGPYARASVSDGMDRSGLSLAENLRRIAGVPLQFAPGTSWG
HHHHHHHCCCCEEEEEECCCCCEEECCCCCCCCCCCHHHHHHHHHHHCCCEEECCCCCCC
YSLAIDVVGALIEAVDGRPLADAVAALVTTPLGMTDTAFVAPDATRFATPYVSTPGAPRR
HHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCCCCCCEEECCCCHHHCCCCCCCCCCCCC
MADVDLVPVFDGTIGIRFEPARAFDVDAWPSGGAGMVGTARDCVTLLDTLRTGRDGWLGR
CCCCEEEEEECCEEEEEECCCCEEECCCCCCCCCCCCCCHHHHHHHHHHHHCCCCCCCCC
AWTDEMARAQPGAHDLRDAPGFGFGLGFSVLRDPVAAQSPESVGTWRGGGAYGHTWFVDR
HHHHHHHHCCCCCHHHCCCCCCCHHHHHHHHHCCHHCCCCCCCCCCCCCCCCCCEEEEEC
AAGLTVVALSNTLYEGLNGRIVTCVRDAVYGVGTGSAA
CCCEEEEEEHHHHHHCCCCCEEEEEHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11472796; 11847270 [H]