Click here to switch to the map view.

The map label for this gene is pbuE [H]

Identifier: 78067388

GI number: 78067388

Start: 3042353

End: 3043564

Strand: Reverse

Name: pbuE [H]

Synonym: Bcep18194_A5919

Alternate gene names: 78067388

Gene position: 3043564-3042353 (Counterclockwise)

Preceding gene: 78067389

Following gene: 78067383

Centisome position: 82.39

GC content: 71.78

Gene sequence:

>1212_bases
ATGAGCGATTGCACGTCGATCGATGACGAACTGGCCGTGCGACCGGTGCAGGCCGGCACGCGCCTGCCGGTCGCGAACCT
GCTGGCGCTCGCGACGGCCGGTTTCATCACGATCCTCACCGAAGCGTTGCCGGCCGGCCTGCTGCCGCTGATGAGCGTCG
ACCTGCGCGTGACCGAGGCGCTGATCGGCCAGCTCGTGACCGTGTATGCACTCGGCTCGATCGTCGCGGCGATTCCGCTC
GTCGCCGCGACGCGCGCGATGCGCCGGCGCAGCCTGCTGCTCGCGGCGCTGGCCGGCTTCGTCGTGTCGAATGCGCTGAC
GGCGGTGTCGCCGTACTACGCGCTCACGCTTGCCGCGCGTTTCGTCGCGGGGATGGCGGCCGGGCTGCTGTGGGCGCTGC
TGGCCGGCTATGCGAGCCGGATGGTCGATGCGTCGCTGCGTGGACGCGCGATCGCGGTCGCGATGCTCGGCGCGCCGGTC
GCGATGTCGATCGGCATTCCGGCCGGCACCGCGTTAGGGGCGGCATTCGGATGGCGCATCGCGTTCGCGGGCATCACGCT
CGCGGCGCTCGTGCTGATCGGCTGGATCCGCTGGCGCGTGCCCGACTATCCGGGGCAACAGGCCGGCGCGCGCGAGCCGG
TGCTCGGCGTGATGACGCTGCCCGGCGTGCGGCCGGTGCTGACCGTGATGTTTGCGTACGTGCTCGCGCACAACATGCTG
TACACCTACGTCGCGCCGTTTCTCGCGGGCGTGCGGATGGGCGCGCAGGTCGATGCGGTGCTGCTCGTGTTCGGTATCGC
GTCGCTGGTCGGCATCGCGCTGACGGGTGCCTGGATCGGCGCTGCGCAGCGACGTCTGACGCTCGTCAGCATCGCGCTGT
TCGCGATCGCGGCGGCGATGCTTGGCGCCGGTTCCGGAATGGCGATCGTCTATGCGGGCGTCGCGCTGTGGGGGCTCGCG
TTCGGCGGCGCGCCGACGCTGTTCCAGACGGCCACCGCGAACGCGGCAGGGGAGGCGGCCGACGTCGCGCAGTCGATGCT
TGTGACGGTGTGGAATCTCGCGATCGCGGGCGGCGGCATCGCGGGCGGGATGCTGCTCGGCGCAACGGGCGCCGGTGCGA
TTCCGTGGGTGCTCGTCGCGCTGCTGGCGGCGGCCTGGGTCGGCGCGTGGCGTGCGCGCTGGCATGCGTTTCCGGCACCG
CACGCGGCTTGA

Upstream 100 bases:

>100_bases
CGGTCGTCGCGCTGTCCAACACGCTGTACGAGGGCCTCAATGGGCGGATCGTGACCTGTGTGCGCGATGCGGTCTACGGC
GTCGGAACCGGGAGCGCGGC

Downstream 100 bases:

>100_bases
GCGTTCGACTGCCGCGCGCGGTGCGCGGCTTCGGCGTCAGGCCGCCTTCGCGTCGAGCGCGGCGCGCGCGCAGGTTTCGT
CGGTGACGAGGCCCGACAGC

Product: major facilitator transporter

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MSDCTSIDDELAVRPVQAGTRLPVANLLALATAGFITILTEALPAGLLPLMSVDLRVTEALIGQLVTVYALGSIVAAIPL
VAATRAMRRRSLLLAALAGFVVSNALTAVSPYYALTLAARFVAGMAAGLLWALLAGYASRMVDASLRGRAIAVAMLGAPV
AMSIGIPAGTALGAAFGWRIAFAGITLAALVLIGWIRWRVPDYPGQQAGAREPVLGVMTLPGVRPVLTVMFAYVLAHNML
YTYVAPFLAGVRMGAQVDAVLLVFGIASLVGIALTGAWIGAAQRRLTLVSIALFAIAAAMLGAGSGMAIVYAGVALWGLA
FGGAPTLFQTATANAAGEAADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLAAAWVGAWRARWHAFPAP
HAA

Sequences:

>Translated_403_residues
MSDCTSIDDELAVRPVQAGTRLPVANLLALATAGFITILTEALPAGLLPLMSVDLRVTEALIGQLVTVYALGSIVAAIPL
VAATRAMRRRSLLLAALAGFVVSNALTAVSPYYALTLAARFVAGMAAGLLWALLAGYASRMVDASLRGRAIAVAMLGAPV
AMSIGIPAGTALGAAFGWRIAFAGITLAALVLIGWIRWRVPDYPGQQAGAREPVLGVMTLPGVRPVLTVMFAYVLAHNML
YTYVAPFLAGVRMGAQVDAVLLVFGIASLVGIALTGAWIGAAQRRLTLVSIALFAIAAAMLGAGSGMAIVYAGVALWGLA
FGGAPTLFQTATANAAGEAADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLAAAWVGAWRARWHAFPAP
HAA
>Mature_402_residues
SDCTSIDDELAVRPVQAGTRLPVANLLALATAGFITILTEALPAGLLPLMSVDLRVTEALIGQLVTVYALGSIVAAIPLV
AATRAMRRRSLLLAALAGFVVSNALTAVSPYYALTLAARFVAGMAAGLLWALLAGYASRMVDASLRGRAIAVAMLGAPVA
MSIGIPAGTALGAAFGWRIAFAGITLAALVLIGWIRWRVPDYPGQQAGAREPVLGVMTLPGVRPVLTVMFAYVLAHNMLY
TYVAPFLAGVRMGAQVDAVLLVFGIASLVGIALTGAWIGAAQRRLTLVSIALFAIAAAMLGAGSGMAIVYAGVALWGLAF
GGAPTLFQTATANAAGEAADVAQSMLVTVWNLAIAGGGIAGGMLLGATGAGAIPWVLVALLAAAWVGAWRARWHAFPAPH
AA

Specific function: Involved in the efflux of purine ribonucleosides, such as guanosine, adenosine and inosine, as well as purine bases guanine, adenine and hypoxanthine, and purine base analogs 2,6- diaminopurine, 6-mercaptopurine and 2-fluoroadenine. Therefore plays a role

COG id: COG2814

COG function: function code G; Arabinose efflux permease

Gene ontology:

Cell location: Cell membrane; Multi-pass membrane protein (Potential) [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. DHA1 family. PbuE (TC 2.A.1.2.25) subfamily [H]

Homologues:

Organism=Escherichia coli, GI145693198, Length=338, Percent_Identity=29.8816568047337, Blast_Score=110, Evalue=1e-25,
Organism=Escherichia coli, GI1787808, Length=226, Percent_Identity=27.4336283185841, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1787947, Length=262, Percent_Identity=32.0610687022901, Blast_Score=80, Evalue=3e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 41017; Mature: 40886

Theoretical pI: Translated: 10.37; Mature: 10.37

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
3.7 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
3.5 %Met     (Mature Protein)
3.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSDCTSIDDELAVRPVQAGTRLPVANLLALATAGFITILTEALPAGLLPLMSVDLRVTEA
CCCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LIGQLVTVYALGSIVAAIPLVAATRAMRRRSLLLAALAGFVVSNALTAVSPYYALTLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVAGMAAGLLWALLAGYASRMVDASLRGRAIAVAMLGAPVAMSIGIPAGTALGAAFGWRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCEEEECCCCCHHHHHHHHHHH
AFAGITLAALVLIGWIRWRVPDYPGQQAGAREPVLGVMTLPGVRPVLTVMFAYVLAHNML
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YTYVAPFLAGVRMGAQVDAVLLVFGIASLVGIALTGAWIGAAQRRLTLVSIALFAIAAAM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAGSGMAIVYAGVALWGLAFGGAPTLFQTATANAAGEAADVAQSMLVTVWNLAIAGGGI
HCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
AGGMLLGATGAGAIPWVLVALLAAAWVGAWRARWHAFPAPHAA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC
>Mature Secondary Structure 
SDCTSIDDELAVRPVQAGTRLPVANLLALATAGFITILTEALPAGLLPLMSVDLRVTEA
CCCCCCCHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCHHHHHHHHHHHHHH
LIGQLVTVYALGSIVAAIPLVAATRAMRRRSLLLAALAGFVVSNALTAVSPYYALTLAAR
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
FVAGMAAGLLWALLAGYASRMVDASLRGRAIAVAMLGAPVAMSIGIPAGTALGAAFGWRI
HHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEHHCCCCEEEECCCCCHHHHHHHHHHH
AFAGITLAALVLIGWIRWRVPDYPGQQAGAREPVLGVMTLPGVRPVLTVMFAYVLAHNML
HHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCEEEEECCCHHHHHHHHHHHHHHHHHH
YTYVAPFLAGVRMGAQVDAVLLVFGIASLVGIALTGAWIGAAQRRLTLVSIALFAIAAAM
HHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
LGAGSGMAIVYAGVALWGLAFGGAPTLFQTATANAAGEAADVAQSMLVTVWNLAIAGGGI
HCCCCCHHHHHHHHHHHHHHCCCCCHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCH
AGGMLLGATGAGAIPWVLVALLAAAWVGAWRARWHAFPAPHAA
HHHHHCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 9202461; 9384377 [H]