| Definition | Burkholderia sp. 383 chromosome 1, complete genome. |
|---|---|
| Accession | NC_007510 |
| Length | 3,694,126 |
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The map label for this gene is amiC [H]
Identifier: 78067364
GI number: 78067364
Start: 3014835
End: 3016361
Strand: Direct
Name: amiC [H]
Synonym: Bcep18194_A5895
Alternate gene names: 78067364
Gene position: 3014835-3016361 (Clockwise)
Preceding gene: 78067363
Following gene: 78067366
Centisome position: 81.61
GC content: 67.85
Gene sequence:
>1527_bases ATGTCTCGAAAGATGTTGATCAAACCGTTCCGCTCGATCGAATCGGCGGCCACCGCGACGCACAACTGGCGGCGCCGCCA GATCCTGTGCGCGGGCGCGTCGACGCTGGTGCTCGGTCTCGTCGCACCGCGGCTCGCGCACGCATCGTCGGTGCTTGGCG TGCGCGTGTGGCCCGCACGCGATTACACGCGCGTCACGATCGAATCCGACCAGCCGCTGCAGAATAGCCAACAGCTGCTG CAGGGTCCCGACCGTCTCGTCGTCGACCTGACCGGCCTCGACCTCGACCAGGCGCTGCGCGACCTCGTGTCGAAGATCGC GCCGAACGATCCGCAGATCCAGTCGGTGCGCGTCGGCCAGTATCAGCCGCACGTCGTACGGATGGTGTTCGACCTGAAAG GCTCGGTGAAGCCGCAGGTGTTCACGCTGCCGCCGGTCGGCACCTACAAGTACCGGCTCGTGTTCGACCTGTATCCGGCC GTCGCACCCGATCCGCTGTCCGACCTGATCGCACAGACGGAACGCAAGGAGCAGGCGCTCAACGACACGACGCGCGCGCA GCAGATGCAGCCGCCGACCGCGCTGGCCGGCCCGGGTACGCCCCTGCCGCCGCCTGCCGCGGGCGACAACAGCGACGCGT TCTTCCAGCGCTTCGCGCAGAACACGCCGGCCACGCCGCACACGCCGCCCGCCACCCCGTCGACGCCCGCGAAGCCGGCC GTCAAGCCGCCGCCCGTCATCGCGCGCCGCGACGACAGCGACGATGACGGCGACACCTACAAGTTCACGGCGCCGAAATC CGGCAAGGGCGGCACCGTGCGCCTGCTGACGGTGGCGATCGATCCGGGCCACGGCGGCGAGGATCCGGGCGCGATCGGCG GCGGTGGCACGTACGAGAAGCACATTGCGCTCGACATCGCAAAGAAGCTGCGCGCGAAGATCGACGGCGCACCGAACATG CGCGCGATGATGACGCGCGACGCCGACTTCTTCGTGCCGCTGAACGTGCGCGTGCAGAAGGCTCGCCGCGTGGGCGCCGA CCTGTTCGTGTCGATCCACGCCGATGCGTTCACGACGCCGTCCGCGCGCGGCTCGTCGGTGTTCGCGCTCTCCGACCACG GCGCGACGAGCGCCGCGGCGCGCTGGATGGCGAACAAGGAAAATTCGTCCGACCTGATCGGCGGCATCAATATCAAGACG GCGGACGCGACCGTGAACCGCGCGCTGTTCGACATGTCGACGACCGCGCAGATCCGCGATTCGCTGCGCTACGGCAACTA CGTGCTGAAGGAAGTGGGCGGCATCAACAAGCTGCACAAGGGCTCGGTCGAGCAGGCCGGGTTCGCGGTGCTGAAGGCGC CCGACATTCCGTCGATCCTCGTCGAGACGGCCTTCATCAGCAACCCGGAAGAAGAGAGCCGCCTGAACGACGACAGCTAT CGCGATCAGATGGCCGACGCGATCTTCCGCGGCATCAAGCGCTATTTCGCCGCGAATCCGCCGCTCGCGAAGAGCCGGAT GGCCTGA
Upstream 100 bases:
>100_bases CGGGCACGCTGCTCGGCGTACCCGATCTGGTTTTCTCGCTCGACGTGGATGGCGACGGCCGCGCCCTCACCGTCCGGGCG TACAGCGCTTCAGGAAAGGC
Downstream 100 bases:
>100_bases CGGCCGCACCCGGCCACCCCGCCCCGCCGCCGGCACGCAGTGCGTGCCGGCGCGTCACGATGCCGCGAAGCGGCGCATCA GTTTCCCGCCGAACACGTTC
Product: N-acetylmuramoyl-L-alanine amidase
Products: NA
Alternate protein names: NA
Number of amino acids: Translated: 508; Mature: 507
Protein sequence:
>508_residues MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLL QGPDRLVVDLTGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPA VAPDPLSDLIAQTERKEQALNDTTRAQQMQPPTALAGPGTPLPPPAAGDNSDAFFQRFAQNTPATPHTPPATPSTPAKPA VKPPPVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNM RAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKT ADATVNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDDSY RDQMADAIFRGIKRYFAANPPLAKSRMA
Sequences:
>Translated_508_residues MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLL QGPDRLVVDLTGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPA VAPDPLSDLIAQTERKEQALNDTTRAQQMQPPTALAGPGTPLPPPAAGDNSDAFFQRFAQNTPATPHTPPATPSTPAKPA VKPPPVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNM RAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKT ADATVNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDDSY RDQMADAIFRGIKRYFAANPPLAKSRMA >Mature_507_residues SRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPARDYTRVTIESDQPLQNSQQLLQ GPDRLVVDLTGLDLDQALRDLVSKIAPNDPQIQSVRVGQYQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAV APDPLSDLIAQTERKEQALNDTTRAQQMQPPTALAGPGTPLPPPAAGDNSDAFFQRFAQNTPATPHTPPATPSTPAKPAV KPPPVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGGGTYEKHIALDIAKKLRAKIDGAPNMR AMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTPSARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTA DATVNRALFDMSTTAQIRDSLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDDSYR DQMADAIFRGIKRYFAANPPLAKSRMA
Specific function: Cell-wall hydrolase involved in septum cleavage during cell division [H]
COG id: COG0860
COG function: function code M; N-acetylmuramoyl-L-alanine amidase
Gene ontology:
Cell location: Periplasm [H]
Metaboloic importance: Non_Essential [C]
Operon status: Not Known
Operon components: None
Similarity: Belongs to the N-acetylmuramoyl-L-alanine amidase 3 family [H]
Homologues:
Organism=Escherichia coli, GI87082163, Length=485, Percent_Identity=40.8247422680412, Blast_Score=329, Evalue=3e-91, Organism=Escherichia coli, GI1788776, Length=234, Percent_Identity=46.5811965811966, Blast_Score=216, Evalue=3e-57, Organism=Escherichia coli, GI1790611, Length=229, Percent_Identity=41.0480349344978, Blast_Score=186, Evalue=3e-48,
Paralogues:
None
Copy number: NA
Swissprot (AC and ID): NA
Other databases:
- InterPro: IPR021731 - InterPro: IPR002508 - InterPro: IPR006311 [H]
Pfam domain/function: PF01520 Amidase_3; PF11741 AMIN [H]
EC number: =3.5.1.28 [H]
Molecular weight: Translated: 54941; Mature: 54810
Theoretical pI: Translated: 9.90; Mature: 9.90
Prosite motif: NA
Important sites: NA
Signals:
None
Transmembrane regions:
None
Cys/Met content:
0.2 %Cys (Translated Protein) 2.2 %Met (Translated Protein) 2.4 %Cys+Met (Translated Protein) 0.2 %Cys (Mature Protein) 2.0 %Met (Mature Protein) 2.2 %Cys+Met (Mature Protein)
Secondary structure:
>Translated Secondary Structure MSRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPAR CCCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCC DYTRVTIESDQPLQNSQQLLQGPDRLVVDLTGLDLDQALRDLVSKIAPNDPQIQSVRVGQ CCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCC YQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQAL CCCHHEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH NDTTRAQQMQPPTALAGPGTPLPPPAAGDNSDAFFQRFAQNTPATPHTPPATPSTPAKPA HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC VKPPPVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGGGTYEK CCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHH HIALDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTP HHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCC SARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADATVNRALFDMSTTAQIRD CCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEECCHHHHHHHHCCCHHHHHHH SLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDDSY HHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHHCCCHHH RDQMADAIFRGIKRYFAANPPLAKSRMA HHHHHHHHHHHHHHHHCCCCCCHHCCCC >Mature Secondary Structure SRKMLIKPFRSIESAATATHNWRRRQILCAGASTLVLGLVAPRLAHASSVLGVRVWPAR CCCHHHHHHHHHHHHHHHHCCCCCEEEEECCHHHHHHHHHHHHHHHHHHEEEEEEECCC DYTRVTIESDQPLQNSQQLLQGPDRLVVDLTGLDLDQALRDLVSKIAPNDPQIQSVRVGQ CCEEEEECCCCCCHHHHHHHCCCCEEEEEEECCCHHHHHHHHHHHHCCCCCCEEEEECCC YQPHVVRMVFDLKGSVKPQVFTLPPVGTYKYRLVFDLYPAVAPDPLSDLIAQTERKEQAL CCCHHEEEEECCCCCCCCEEEECCCCCCEEEEEEEEECCCCCCCCHHHHHHHHHHHHHHH NDTTRAQQMQPPTALAGPGTPLPPPAAGDNSDAFFQRFAQNTPATPHTPPATPSTPAKPA HHHHHHHHCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCCCCCCCCCCCCCCCCCCC VKPPPVIARRDDSDDDGDTYKFTAPKSGKGGTVRLLTVAIDPGHGGEDPGAIGGGGTYEK CCCCCEEEECCCCCCCCCEEEEECCCCCCCCEEEEEEEEECCCCCCCCCCCCCCCCCCHH HIALDIAKKLRAKIDGAPNMRAMMTRDADFFVPLNVRVQKARRVGADLFVSIHADAFTTP HHHHHHHHHHHHHCCCCCCCEEEEECCCCEEEECCHHHHHHHHCCCEEEEEEECCCCCCC SARGSSVFALSDHGATSAAARWMANKENSSDLIGGINIKTADATVNRALFDMSTTAQIRD CCCCCEEEEEECCCCHHHHHHHHCCCCCCCCEEECCEEEECCHHHHHHHHCCCHHHHHHH SLRYGNYVLKEVGGINKLHKGSVEQAGFAVLKAPDIPSILVETAFISNPEEESRLNDDSY HHHHHHHHHHHHCCCCHHCCCCCHHCCEEEEECCCCCHHHHHHHHCCCCCHHHHCCCHHH RDQMADAIFRGIKRYFAANPPLAKSRMA HHHHHHHHHHHHHHHHCCCCCCHHCCCC
PDB accession: NA
Resolution: NA
Structure class: Alpha Beta
Cofactors: NA
Metal ions: NA
Kcat value (1/min): NA
Specific activity: NA
Km value (mM): NA
Substrates: NA
Specific reaction: NA
General reaction: NA
Inhibitor: NA
Structure determination priority: 9.0
TargetDB status: NA
Availability: NA
References: 12471157 [H]