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The map label for this gene is gltX

Identifier: 78066834

GI number: 78066834

Start: 2426327

End: 2427865

Strand: Reverse

Name: gltX

Synonym: Bcep18194_A5365

Alternate gene names: 78066834

Gene position: 2427865-2426327 (Counterclockwise)

Preceding gene: 78066836

Following gene: 78066833

Centisome position: 65.72

GC content: 66.41

Gene sequence:

>1539_bases
ATGTCCGGCGCGAGTATAATTCCGGCTTCTTTCCCGTTCCGGGCGTCGCCGGCCCCGTTGCTGTCCGGGCTGCCGCCGTC
GATCCCGCGTCGAATCATTCATGCGCCCCAGGCGTTCGAGTTACCGTTTATGACCCGTCCTGTCCGTACCCGCTTCGCGC
CGAGCCCCACCGGCTTCATCCACCTCGGCAACATCCGCTCCGCACTCTATCCGTGGGCGTTCGCCCGCAAGATGAAGGGC
ACGTTCGTGCTGCGCATCGAGGATACCGACGTCGAGCGCTCGTCGCAGGAAGCAGTCGACGCGATCCTCGATGGGATGCA
GTGGCTCGGCCTGGATTTCGACGAAGGCCCGATCTATCAGATGCAGCGGATGGACCGCTATCGCGAGGTGATCGCGCAGA
TGCTCGAAAAGGGCCTCGCGTACCCGTGCTACATGTCGGCCGAGGAACTCGACGCGCTGCGCGAACGCCAGCGCGAGGCC
GGCCTGAAGCCGCGCTATGACGGCACGTGGCGTCCGGAGCCCGGCAAGGTGCTGCCGGAGCCGCCGGCCGGCGTGAAGCC
GGTGCTGCGCTTCCGCAATCCGCTGACCGGCACGGTCGTGTGGGACGACGCCGTGAAGGGGCGTGTCGAGATCTCGAATG
AAGAGCTCGACGACCTCGTGATCGCGCGCCCGGACGGCACGCCGATCTACAACTTCTGCGTGGTGGTGGACGACATGGAC
ATGGGCATAACGCACGTGATCCGTGGCGATGACCACGTGAACAACACGCCGCGCCAGATCAACATCCTGAATGCGCTCGG
CGGCGAGCCGCCCGTCTACGCGCACCTGCCGACCGTGCTGAACGAGCAGGGCGAGAAGATGAGCAAGCGCCATGGCGCGA
TGAGCGTGATGGCGTACCGCGACGCGGGCTTCCTGCCTGAAGCCGTCGTCAACTATCTCGCGCGCCTCGGCTGGTCGCAC
GGCGACGCCGAGATCTTCTCGCGCGAGCAGTTCGTCGAATGGTTCGATCTCGAGCATCTCGGCAAGTCGCCCGCGCAGTA
CGACCACAGCAAGCTGAGCTGGCTGAACGCGCATTACATCAAGGAAGCGGACAACGCGCGCCTCGCCGAGCTGGCGAAGC
CGTTCCTCGCCGCCCTCGGCATCGACGATGCGGCGATCGCGACGGGCCCTGCGCTCGAAGCGGTGGTCGGGCTGATGAAG
GATCGCGCGACGACGGTCAAGGAGATCGCGGAAGGCGCCACGATGTTCTATCGTGTGCCGGCACCGGAAGCCGATGCGCT
TGCACAGCACGTGACCGATGTGGTGCGTCCGGCGCTGGCCGATCTCGTCGCTGCGCTGAAGGCGGCCGACTGGACCAAGG
AGGCCGTGTCCGCCGCGCTGAAGGCGACGCTCGGCACGCACAAGCTGAAGATGCCGCAACTCGCGATGCCGGTGCGCCTG
CTGGTGGCCGGTACGACGCACACGCCGTCGATCGATGCGGTGCTCGTGCTGTTCGGTCGCGATGTCGTGGTGTCGCGCAT
CGAGGCCGCGCTGGCCTGA

Upstream 100 bases:

>100_bases
TAGGTTCCGAGCGACCGTACGGCAGGTTCCGTATGGCCGGCGCCGCGCCGGCTGGCGGCCGCGGCGCGCACATCATAAAG
CAAGCGGCGCGCTTGGCGCG

Downstream 100 bases:

>100_bases
GGTTCGAGGGCGAGCGAAGCTGCCTGTGCGTGACATCGCGGCAGCTTCGCAAAAAATTTCGCTCGAATCGCAAAAAGGGT
ATTTACAAGTTCAAAGTAGC

Product: glutamyl-tRNA synthetase

Products: NA

Alternate protein names: Glutamate--tRNA ligase; GluRS [H]

Number of amino acids: Translated: 512; Mature: 511

Protein sequence:

>512_residues
MSGASIIPASFPFRASPAPLLSGLPPSIPRRIIHAPQAFELPFMTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKG
TFVLRIEDTDVERSSQEAVDAILDGMQWLGLDFDEGPIYQMQRMDRYREVIAQMLEKGLAYPCYMSAEELDALRERQREA
GLKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMD
MGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQGEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSH
GDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEADNARLAELAKPFLAALGIDDAAIATGPALEAVVGLMK
DRATTVKEIAEGATMFYRVPAPEADALAQHVTDVVRPALADLVAALKAADWTKEAVSAALKATLGTHKLKMPQLAMPVRL
LVAGTTHTPSIDAVLVLFGRDVVVSRIEAALA

Sequences:

>Translated_512_residues
MSGASIIPASFPFRASPAPLLSGLPPSIPRRIIHAPQAFELPFMTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKG
TFVLRIEDTDVERSSQEAVDAILDGMQWLGLDFDEGPIYQMQRMDRYREVIAQMLEKGLAYPCYMSAEELDALRERQREA
GLKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMD
MGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQGEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSH
GDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEADNARLAELAKPFLAALGIDDAAIATGPALEAVVGLMK
DRATTVKEIAEGATMFYRVPAPEADALAQHVTDVVRPALADLVAALKAADWTKEAVSAALKATLGTHKLKMPQLAMPVRL
LVAGTTHTPSIDAVLVLFGRDVVVSRIEAALA
>Mature_511_residues
SGASIIPASFPFRASPAPLLSGLPPSIPRRIIHAPQAFELPFMTRPVRTRFAPSPTGFIHLGNIRSALYPWAFARKMKGT
FVLRIEDTDVERSSQEAVDAILDGMQWLGLDFDEGPIYQMQRMDRYREVIAQMLEKGLAYPCYMSAEELDALRERQREAG
LKPRYDGTWRPEPGKVLPEPPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMDM
GITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQGEKMSKRHGAMSVMAYRDAGFLPEAVVNYLARLGWSHG
DAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYIKEADNARLAELAKPFLAALGIDDAAIATGPALEAVVGLMKD
RATTVKEIAEGATMFYRVPAPEADALAQHVTDVVRPALADLVAALKAADWTKEAVSAALKATLGTHKLKMPQLAMPVRLL
VAGTTHTPSIDAVLVLFGRDVVVSRIEAALA

Specific function: Catalyzes the attachment of glutamate to tRNA(Glu) in a two-step reaction:glutamate is first activated by ATP to form Glu-AMP and then transferred to the acceptor end of tRNA(Glu) [H]

COG id: COG0008

COG function: function code J; Glutamyl- and glutaminyl-tRNA synthetases

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the class-I aminoacyl-tRNA synthetase family [H]

Homologues:

Organism=Homo sapiens, GI134288884, Length=494, Percent_Identity=29.5546558704453, Blast_Score=204, Evalue=2e-52,
Organism=Escherichia coli, GI1788743, Length=468, Percent_Identity=50.2136752136752, Blast_Score=466, Evalue=1e-132,
Organism=Escherichia coli, GI226510925, Length=245, Percent_Identity=36.734693877551, Blast_Score=142, Evalue=4e-35,
Organism=Escherichia coli, GI1786895, Length=207, Percent_Identity=25.6038647342995, Blast_Score=69, Evalue=9e-13,
Organism=Caenorhabditis elegans, GI71993394, Length=481, Percent_Identity=29.3139293139293, Blast_Score=186, Evalue=2e-47,
Organism=Caenorhabditis elegans, GI17543332, Length=130, Percent_Identity=30.7692307692308, Blast_Score=69, Evalue=4e-12,
Organism=Saccharomyces cerevisiae, GI6324540, Length=332, Percent_Identity=36.7469879518072, Blast_Score=220, Evalue=5e-58,
Organism=Saccharomyces cerevisiae, GI6321192, Length=233, Percent_Identity=26.1802575107296, Blast_Score=76, Evalue=1e-14,
Organism=Saccharomyces cerevisiae, GI6324742, Length=123, Percent_Identity=32.520325203252, Blast_Score=69, Evalue=3e-12,
Organism=Drosophila melanogaster, GI24665321, Length=498, Percent_Identity=29.3172690763052, Blast_Score=218, Evalue=9e-57,

Paralogues:

None

Copy number: 1,800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR008925
- InterPro:   IPR020751
- InterPro:   IPR001412
- InterPro:   IPR004527
- InterPro:   IPR000924
- InterPro:   IPR020061
- InterPro:   IPR020058
- InterPro:   IPR014729 [H]

Pfam domain/function: PF00749 tRNA-synt_1c [H]

EC number: =6.1.1.17 [H]

Molecular weight: Translated: 56405; Mature: 56274

Theoretical pI: Translated: 6.07; Mature: 6.07

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.5 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSGASIIPASFPFRASPAPLLSGLPPSIPRRIIHAPQAFELPFMTRPVRTRFAPSPTGFI
CCCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEE
HLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILDGMQWLGLDFDEGPIYQ
EECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHH
MQRMDRYREVIAQMLEKGLAYPCYMSAEELDALRERQREAGLKPRYDGTWRPEPGKVLPE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMD
CCCCCHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEECCCCCCEEEEEEEEECCC
MGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQGEKMSKRHGAMSVMAYR
CCEEEEECCCCCCCCCCCEEEEHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEEEEC
DAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYI
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KEADNARLAELAKPFLAALGIDDAAIATGPALEAVVGLMKDRATTVKEIAEGATMFYRVP
HCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
APEADALAQHVTDVVRPALADLVAALKAADWTKEAVSAALKATLGTHKLKMPQLAMPVRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHCCEEE
LVAGTTHTPSIDAVLVLFGRDVVVSRIEAALA
EEECCCCCCCCCEEEEECCCHHHHHHHHHHCC
>Mature Secondary Structure 
SGASIIPASFPFRASPAPLLSGLPPSIPRRIIHAPQAFELPFMTRPVRTRFAPSPTGFI
CCCCCCCCCCCCCCCCCHHHCCCCCHHHHHHHCCCCCCCCCCCCCCHHHCCCCCCCCEE
HLGNIRSALYPWAFARKMKGTFVLRIEDTDVERSSQEAVDAILDGMQWLGLDFDEGPIYQ
EECCHHHHHHHHHHHHHCCCEEEEEEECCCCCCHHHHHHHHHHCCHHHHCCCCCCCCHHH
MQRMDRYREVIAQMLEKGLAYPCYMSAEELDALRERQREAGLKPRYDGTWRPEPGKVLPE
HHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCC
PPAGVKPVLRFRNPLTGTVVWDDAVKGRVEISNEELDDLVIARPDGTPIYNFCVVVDDMD
CCCCCHHHHHHCCCCCEEEEECCCCCCEEEECCCCCCCEEEECCCCCCEEEEEEEEECCC
MGITHVIRGDDHVNNTPRQINILNALGGEPPVYAHLPTVLNEQGEKMSKRHGAMSVMAYR
CCEEEEECCCCCCCCCCCEEEEHHHCCCCCCEEEECCHHHHHHHHHHHHHHCCEEEEEEC
DAGFLPEAVVNYLARLGWSHGDAEIFSREQFVEWFDLEHLGKSPAQYDHSKLSWLNAHYI
CCCCCHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHH
KEADNARLAELAKPFLAALGIDDAAIATGPALEAVVGLMKDRATTVKEIAEGATMFYRVP
HCCCCHHHHHHHHHHHHHHCCCCHHHCCCHHHHHHHHHHHHHHHHHHHHHCCCEEEEECC
APEADALAQHVTDVVRPALADLVAALKAADWTKEAVSAALKATLGTHKLKMPQLAMPVRL
CCCHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHCCCCCCCCHHHCCEEE
LVAGTTHTPSIDAVLVLFGRDVVVSRIEAALA
EEECCCCCCCCCEEEEECCCHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA