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The map label for this gene is rluF [H]

Identifier: 78066801

GI number: 78066801

Start: 2385955

End: 2387826

Strand: Direct

Name: rluF [H]

Synonym: Bcep18194_A5332

Alternate gene names: 78066801

Gene position: 2385955-2387826 (Clockwise)

Preceding gene: 78066800

Following gene: 78066807

Centisome position: 64.59

GC content: 70.67

Gene sequence:

>1872_bases
ATGCGCACCAAATTAACCGTCAAGAATCCGAAGCCGGCGTCGCCGACCCGCGCCCCTGTCCGCTCCGGCAGCCTCGTCGC
ACGCAAGGCGGTACGCCCCGCTGCCCCGCCCGCGGGGGACAAGCCGGCCCGCCCGAAGAAGGCGTCCCCGGCCGCCGCCG
GCGACCGCCCGTTCAAGCCGCGCGCCGCCGCGGGCGCCGAACGCCCGGGCGCGGATCGCGCCCCGGCCAAGCGTGCGCCG
CGTGACGGCGGTGCCGAACGCGGCACGCGTGCCCCTTATCGCGACAACGCGGCCGGTGAAGGCGCGAAGCGCAGCTTCGG
CGATCGTCGCACGTCGTCCGATCGCCCGCCGCGTCGCGATGACGATGCACGTCCGCGTCGTGCGGGCTCCAGTGAAGGCG
GCGCACGCGCACCTTATCGTGACAACGCATCCGGCGAAGGCGCAAAGCGCAGCTTCGGCGACCGCCGTACGTCGTCCGAT
CGCCCGCCGCGTCGTGATGACGATGCACGTCCGCGTCGCGCAGGTTCCAGTGAAGGCGGCGCACGCGCACCTTATCGCGA
CAACGCCGGTGGCGAAGGCGCAAAGCGCAGCTTCGGTGACCGTCGCCCGTCGTCCGATCGCCCGCCGCGTCGCGATGACG
ACTCACGGCCGCGTCGCGCGGGCAGCGAAGGCGGTGCACGTGCACCGTATCGCGACAACGCAGGCGGTGAAGGTGCAAAA
CGCAGCTTCGGCGACCGCCGCACGTCGTCGGATCGTCCGCCGCGTCGCGATGACGACTCACGGCCGCGTCGCGCAGGTTC
CAGTGAAGGCGGCGCACGCGCACCTTATCGCGACAACGCAGCCGGCGAAGGCGCAAAACGCAGCTTCGGCGACCGCCGCA
CGTCGTCCGATCGCCCGCCGCGCCGCAACGACGATGACCGACCGCGCCGCGCAGGCGCCGACGACGGCAAGCGCCCGTCG
TATCGCGACAAGCCTGCCGGCGAAGGTGCGAAGCGCAGCTTCGGCGACCGCCCGGCCCGCCCCGCACGCGACGGCGAGCG
TCGCTCGTTCGGTGCGGTCAAGACCGCGCAACCGGTCAAGCGCGCCTCGGCCGACGTCGACTACGGCGACGAAACGGGCC
TGATGCGCCTGTCGAAGCGGATGTCCGAGCTCGGCATGTGCTCGCGCCGCGAAGCTGACGAATGGATCGAAAAGGGCTGG
GTGCTTGTCGACGGCGAACGCATCGACACGCTCGGCACCAAGGTCCGCGCCGACCAGAAAATCGAGATCGACGAACGGGC
CAGCGCCGCGCAGGCCGCGCAAGTGACGATCCTGCTGCACAAGCCGGTCGGTTACGTGTCGGGCCAGGCGGAAGACGGCT
ACGAGCCCGCCGCCGTGCTGATCACGCGCGCGAACCGCTGGAGCGGCGACCATTCGCCGGTGCGCTTCTCGCCGCAGCAC
CTGCACGCACTCGCACCGGCCGGCCGCCTGGACATCGATTCGACCGGCCTGCTCGTGCTGACGCAGAACGGCGTGGTCGC
GAAACAGCTGATCGGCGAGCAGTCGGACATCGACAAGGAGTACCTGGTGCGCGTGCGCTTCGGCGAACGGCTGATCGACA
TCGACCAGCACTTCCCCGCGGAATCGCTCGCGAAGCTGCGTCACGGCCTCGAGCTCGACGGCGTCGCGCTGAAGCCCGCG
ATGGTGAGCTGGCAGAACGGCGAGCAGTTGCGCTTCGTGCTGCGCGAAGGCAAGAAACGCCAGATCCGCCGCATGTGCGA
ACTGGTGGGCCTCGACGTGATCGGCCTGAAGCGCGTGCGGATGGGCCGCGTGATGCTCGGCGCGCTGCCGCAGGGACAAT
GGCGCTATCTGTCGGCCGACGAGACGTTCTGA

Upstream 100 bases:

>100_bases
TTCCTGCTTGCGGGTGCCGGCCTGTCCGACAACCGCCTGCAGATCCAGCTCGAAACCGAATTGCTGCGTGCGATCGCAGT
CTGAATGAATTCCAGCGTTT

Downstream 100 bases:

>100_bases
CCGCGAGCGCACACACGTGCGCCACGCAATGAAAAAGCCCGCGCAAGCGGGCTTTTTTGTTTCCTGCGAGAGACCTGGAA
CGCCGGGCGATCAGTCGTCG

Product: pseudouridine synthase, Rsu

Products: NA

Alternate protein names: rRNA pseudouridylate synthase F; rRNA-uridine isomerase F [H]

Number of amino acids: Translated: 623; Mature: 623

Protein sequence:

>623_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKASPAAAGDRPFKPRAAAGAERPGADRAPAKRAP
RDGGAERGTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGSSEGGARAPYRDNASGEGAKRSFGDRRTSSD
RPPRRDDDARPRRAGSSEGGARAPYRDNAGGEGAKRSFGDRRPSSDRPPRRDDDSRPRRAGSEGGARAPYRDNAGGEGAK
RSFGDRRTSSDRPPRRDDDSRPRRAGSSEGGARAPYRDNAAGEGAKRSFGDRRTSSDRPPRRNDDDRPRRAGADDGKRPS
YRDKPAGEGAKRSFGDRPARPARDGERRSFGAVKTAQPVKRASADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGW
VLVDGERIDTLGTKVRADQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQH
LHALAPAGRLDIDSTGLLVLTQNGVVAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPA
MVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVRMGRVMLGALPQGQWRYLSADETF

Sequences:

>Translated_623_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKASPAAAGDRPFKPRAAAGAERPGADRAPAKRAP
RDGGAERGTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGSSEGGARAPYRDNASGEGAKRSFGDRRTSSD
RPPRRDDDARPRRAGSSEGGARAPYRDNAGGEGAKRSFGDRRPSSDRPPRRDDDSRPRRAGSEGGARAPYRDNAGGEGAK
RSFGDRRTSSDRPPRRDDDSRPRRAGSSEGGARAPYRDNAAGEGAKRSFGDRRTSSDRPPRRNDDDRPRRAGADDGKRPS
YRDKPAGEGAKRSFGDRPARPARDGERRSFGAVKTAQPVKRASADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGW
VLVDGERIDTLGTKVRADQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQH
LHALAPAGRLDIDSTGLLVLTQNGVVAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPA
MVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVRMGRVMLGALPQGQWRYLSADETF
>Mature_623_residues
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKASPAAAGDRPFKPRAAAGAERPGADRAPAKRAP
RDGGAERGTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGSSEGGARAPYRDNASGEGAKRSFGDRRTSSD
RPPRRDDDARPRRAGSSEGGARAPYRDNAGGEGAKRSFGDRRPSSDRPPRRDDDSRPRRAGSEGGARAPYRDNAGGEGAK
RSFGDRRTSSDRPPRRDDDSRPRRAGSSEGGARAPYRDNAAGEGAKRSFGDRRTSSDRPPRRNDDDRPRRAGADDGKRPS
YRDKPAGEGAKRSFGDRPARPARDGERRSFGAVKTAQPVKRASADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGW
VLVDGERIDTLGTKVRADQKIEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQH
LHALAPAGRLDIDSTGLLVLTQNGVVAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPAESLAKLRHGLELDGVALKPA
MVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVRMGRVMLGALPQGQWRYLSADETF

Specific function: Responsible for synthesis of pseudouridine from uracil- 2604 in 23S ribosomal RNA [H]

COG id: COG1187

COG function: function code J; 16S rRNA uridine-516 pseudouridylate synthase and related pseudouridylate synthases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1790453, Length=251, Percent_Identity=35.4581673306773, Blast_Score=118, Evalue=1e-27,
Organism=Escherichia coli, GI1788510, Length=251, Percent_Identity=29.8804780876494, Blast_Score=76, Evalue=7e-15,
Organism=Escherichia coli, GI1787524, Length=262, Percent_Identity=30.5343511450382, Blast_Score=75, Evalue=1e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020103
- InterPro:   IPR006145
- InterPro:   IPR000748
- InterPro:   IPR018496
- InterPro:   IPR002942 [H]

Pfam domain/function: PF00849 PseudoU_synth_2; PF01479 S4 [H]

EC number: NA

Molecular weight: Translated: 68101; Mature: 68101

Theoretical pI: Translated: 11.27; Mature: 11.27

Prosite motif: PS50889 S4

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.6 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKASPAAAGDRPFKP
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAAAGAERPGADRAPAKRAPRDGGAERGTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRD
HHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DDARPRRAGSSEGGARAPYRDNASGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGSSEGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ARAPYRDNAGGEGAKRSFGDRRPSSDRPPRRDDDSRPRRAGSEGGARAPYRDNAGGEGAK
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHH
RSFGDRRTSSDRPPRRDDDSRPRRAGSSEGGARAPYRDNAAGEGAKRSFGDRRTSSDRPP
HHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
RRNDDDRPRRAGADDGKRPSYRDKPAGEGAKRSFGDRPARPARDGERRSFGAVKTAQPVK
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHCCCCHHHHHHH
RASADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGWVLVDGERIDTLGTKVRADQK
HHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC
IEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQH
CCHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHH
LHALAPAGRLDIDSTGLLVLTQNGVVAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPA
HHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEHHCCHHEEHHHCCCH
ESLAKLRHGLELDGVALKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVR
HHHHHHHHCCCCCCEEECCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
MGRVMLGALPQGQWRYLSADETF
HHHHHHHCCCCCCEEEECCCCCC
>Mature Secondary Structure
MRTKLTVKNPKPASPTRAPVRSGSLVARKAVRPAAPPAGDKPARPKKASPAAAGDRPFKP
CCCCEECCCCCCCCCCCCCCCCCCHHHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
RAAAGAERPGADRAPAKRAPRDGGAERGTRAPYRDNAAGEGAKRSFGDRRTSSDRPPRRD
HHHCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCC
DDARPRRAGSSEGGARAPYRDNASGEGAKRSFGDRRTSSDRPPRRDDDARPRRAGSSEGG
CCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCCC
ARAPYRDNAGGEGAKRSFGDRRPSSDRPPRRDDDSRPRRAGSEGGARAPYRDNAGGEGAK
CCCCCCCCCCCCHHHHHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCHHH
RSFGDRRTSSDRPPRRDDDSRPRRAGSSEGGARAPYRDNAAGEGAKRSFGDRRTSSDRPP
HHCCCCCCCCCCCCCCCCCCCCHHCCCCCCCCCCCCCCCCCCCCHHHHCCCCCCCCCCCC
RRNDDDRPRRAGADDGKRPSYRDKPAGEGAKRSFGDRPARPARDGERRSFGAVKTAQPVK
CCCCCCCCHHCCCCCCCCCCCCCCCCCCCHHHCCCCCCCCCCCCCCHHHCCCCHHHHHHH
RASADVDYGDETGLMRLSKRMSELGMCSRREADEWIEKGWVLVDGERIDTLGTKVRADQK
HHCCCCCCCCCHHHHHHHHHHHHHCCHHHHHHHHHHHCCCEEECCCCHHHHHHHHCCCCC
IEIDERASAAQAAQVTILLHKPVGYVSGQAEDGYEPAAVLITRANRWSGDHSPVRFSPQH
CCHHHHHHHHHHEEEEEEEECCCCCCCCCCCCCCCCEEEEEEECCCCCCCCCCEEECHHH
LHALAPAGRLDIDSTGLLVLTQNGVVAKQLIGEQSDIDKEYLVRVRFGERLIDIDQHFPA
HHHHCCCCCCCCCCCCEEEEECCCHHHHHHHCCCCCCCHHHEEEEHHCCHHEEHHHCCCH
ESLAKLRHGLELDGVALKPAMVSWQNGEQLRFVLREGKKRQIRRMCELVGLDVIGLKRVR
HHHHHHHHCCCCCCEEECCCEEECCCCHHHHHHHHCCHHHHHHHHHHHHCCCHHHHHHHH
MGRVMLGALPQGQWRYLSADETF
HHHHHHHCCCCCCEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]