Click here to switch to the map view.

The map label for this gene is smpB

Identifier: 78066778

GI number: 78066778

Start: 2360458

End: 2360904

Strand: Direct

Name: smpB

Synonym: Bcep18194_A5309

Alternate gene names: 78066778

Gene position: 2360458-2360904 (Clockwise)

Preceding gene: 78066775

Following gene: 78066782

Centisome position: 63.9

GC content: 61.52

Gene sequence:

>447_bases
ATGAGCATCATCGACAACAGGAAAGCGCACTTCGATTATCACATCGAGGAACGCTACGAGGCGGGGCTCGTGCTCGAGGG
CTGGGAAGTCAAGGCGCTGCGCGCCGGGCGCGGCCAGATCAGGGAAGGTTACGTCGTGATCAAGCACGACGAGATCTTCC
TGATCGGTACCCATATCAGCCCGCTGCCCGAGGCTTCGACCCACATCACGCCCGACCCGGTTCGCACGCGCAAGCTGCTG
CTGCACCGCGAGGAAATCAAGAAACTGATCGGCAAGGTCGAGCAGCGTGGCTACACGCTCGTGCCGCTGAACTTCCACTA
CAAGGGCGGTCGCGTGAAGTGCGACATCGCGCTCGCGAAGGGCAAGAAGCTGCACGACAAGCGCGAAACCGAGAAGAAAC
GCGACTGGGAACGCGAAAAGGCACGCATCATGCGCGCCGGCACCTGA

Upstream 100 bases:

>100_bases
ATAAATCGCGCGGCATCGCTTAAGGTGAGAACGCAAGCCGGGCGATTCCCGCCCCGCTTTGCTAAAATCGTGATTTTAAA
CGAGTTGGCCCGATCCTTTC

Downstream 100 bases:

>100_bases
CCTGCGCGACACGCATCACAAACGACACGGCCCGCTCGAAGCGGGCCGTGTTGCTTTGGGCGATGTGAATCTGGCAGGCC
GGCTGCCCGGCAGCGGCCGG

Product: SsrA-binding protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 148; Mature: 147

Protein sequence:

>148_residues
MSIIDNRKAHFDYHIEERYEAGLVLEGWEVKALRAGRGQIREGYVVIKHDEIFLIGTHISPLPEASTHITPDPVRTRKLL
LHREEIKKLIGKVEQRGYTLVPLNFHYKGGRVKCDIALAKGKKLHDKRETEKKRDWEREKARIMRAGT

Sequences:

>Translated_148_residues
MSIIDNRKAHFDYHIEERYEAGLVLEGWEVKALRAGRGQIREGYVVIKHDEIFLIGTHISPLPEASTHITPDPVRTRKLL
LHREEIKKLIGKVEQRGYTLVPLNFHYKGGRVKCDIALAKGKKLHDKRETEKKRDWEREKARIMRAGT
>Mature_147_residues
SIIDNRKAHFDYHIEERYEAGLVLEGWEVKALRAGRGQIREGYVVIKHDEIFLIGTHISPLPEASTHITPDPVRTRKLLL
HREEIKKLIGKVEQRGYTLVPLNFHYKGGRVKCDIALAKGKKLHDKRETEKKRDWEREKARIMRAGT

Specific function: Binds specifically to the ssrA RNA (tmRNA) and is required for stable association of ssrA with ribosomes [H]

COG id: COG0691

COG function: function code O; tmRNA-binding protein

Gene ontology:

Cell location: Cytoplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the smpB family [H]

Homologues:

Organism=Escherichia coli, GI1788973, Length=144, Percent_Identity=50, Blast_Score=156, Evalue=6e-40,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000037
- InterPro:   IPR020081
- ProDom:   PD004488 [H]

Pfam domain/function: PF01668 SmpB [H]

EC number: NA

Molecular weight: Translated: 17245; Mature: 17114

Theoretical pI: Translated: 10.30; Mature: 10.30

Prosite motif: PS01317 SSRP

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
1.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSIIDNRKAHFDYHIEERYEAGLVLEGWEVKALRAGRGQIREGYVVIKHDEIFLIGTHIS
CCCCCCCCCEEEEEECCCCCCCEEEECCEEEEEECCCCCCCCCEEEEEECCEEEEECCCC
PLPEASTHITPDPVRTRKLLLHREEIKKLIGKVEQRGYTLVPLNFHYKGGRVKCDIALAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEEECC
GKKLHDKRETEKKRDWEREKARIMRAGT
CCCCCHHHHHHHHHHHHHHHHHHCCCCC
>Mature Secondary Structure 
SIIDNRKAHFDYHIEERYEAGLVLEGWEVKALRAGRGQIREGYVVIKHDEIFLIGTHIS
CCCCCCCCEEEEEECCCCCCCEEEECCEEEEEECCCCCCCCCEEEEEECCEEEEECCCC
PLPEASTHITPDPVRTRKLLLHREEIKKLIGKVEQRGYTLVPLNFHYKGGRVKCDIALAK
CCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEEEECCCEEEEEEEECC
GKKLHDKRETEKKRDWEREKARIMRAGT
CCCCCHHHHHHHHHHHHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA