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The map label for this gene is oprM [H]

Identifier: 78066506

GI number: 78066506

Start: 2074249

End: 2075814

Strand: Direct

Name: oprM [H]

Synonym: Bcep18194_A5037

Alternate gene names: 78066506

Gene position: 2074249-2075814 (Clockwise)

Preceding gene: 78066505

Following gene: 78066507

Centisome position: 56.15

GC content: 71.84

Gene sequence:

>1566_bases
ATGGATCGATCGATGAACCTGAAAACCCAAGCCGTGCGGGCACTCGCCGTGGCGAGCCTCGCCGGCCCGCTCGCGGGCTG
TTCGTGGTTCGCGCCGAGCGGCGAGCCGCCCGCGATGCCGTCGCCCGCGCACTACGGCACCACACCGCAAGTCCAGCAGA
CCGTGTCCGCGCAAGGTGTCGCGCAGCAGTTCGAGGTCGGCGCGCAGCCGGTGCCCGACTGGTGGAAGCAGTACCGCTCC
GATGCACTGAACGCGCTCGTCGACGAAGGGCTGCGCAACAGCCCGACGTTGAGCGCGGCATCGCACTCGCTCGATGCCGC
GCGCGAACAGTTGCGCGGGCAGATCGGCAGCTCGATGCTGCCGTCGATCGATGCGGGCGGCCAGGCCACGCGCCAGCGGG
CGCTCGGCGTGCCGATTCCCGCGCTCGGTGCGCCGACGCTGCTGTACGACACGTTCGTCGGGCAACTGCAGGCGAGCTAC
ACGATCGACCTGTTCGGCGCGTCGCGTTTCGCGAACCGTGCGCTGGCGCGGCGCGTCGACGTCAGCGCGTTCCAGCTCGA
ATCCGCCCGGCGCGCGCTGGCCGCGAACATCGTCACCGCATCGATCACGGTGTCGGTGCTGAATGCGCAGATCAATACCA
CCGAGCGGCTCGTCGCGCTCGCCAACGATCAGGCACACGATGCCGAACGCCGCTACGCGCTCGGTTCGGCCTCGCGCAGC
GACGCGCTGAATGCACGGCAAAGCGCCGACACGTTCGCGGCGAGCCTGCCCGCGCTGCGCCAGCAACGCGACTCGGCGCG
CCATGCGCTCGCGGTGCTGGTCGGCCGCACGCCCGACCAGCCGCCGGCCGACCTCACGCTTGCCGACCTGCACCTGCCCG
AGCAAGTGCCCGTCGTCGTGCCGTCGGACCTGCTGCAAAGCCGCCCCGACATCCAGGCGGCCGACGCGGGGCTGAAGGCG
GCCGCGGCCGAAGTGGGCCTCGCGACCGCGCAGATGTTCCCGCAGCTGTCGCTGTCGGCCGCGATGGGCAAAGGCGGCTT
CAGCTGGCCGACGATGCTTTCGGGCGCCGGCTCGATCTGGAACGTCGGCGCGTCGCTGAGCCAGCCGATCTTCCACGGCG
GCGCGCTGCTCGCGCAGCGCCGCGCAGCGAAGGCGACCTACGAGGCCGCCGTCGACCAGTACAAGCAGGCCGTGCTCGGC
GCGTTCCAGAACGTCGCCGATTCGCTCGCGGCGCTCGAGCACGATGCGGAAGCGCTCGACGCGTCGTCGCGCGCCGCACT
GTCCGCGCGGGGTGCGTATGACGACGCGGCGGCCCGCGTGCGGCTCGGCGCACTGCCGCCGTCGGCCGCGCGCGCCAGCG
AGCTGCAGTATCGCAATGCACGGCTCGACGAGATCCGCGCGACCGGCGCGCGGTTCGCCGATACCGCGAGGCTTTACCAG
GCGATGGGCACGCCGCCGGTCGAGAAGGCTGACAAGGCCGGCAAGACCGACAAGGCAACCGGCGACTCCGCCACCGGCCA
GCAAGCGCGCGCCGCCACGGCCGACACCCCGCCTTCGCCGCAATAA

Upstream 100 bases:

>100_bases
CGCTGTATGCGACGTGGTTCCGCGTGAAGCGCGACGAACGCGACCCGCAGGACGGCCCGCCGCCTGGCGGCACGCCTGCC
GCGCCGTCGGGAGCCTGACC

Downstream 100 bases:

>100_bases
GCCGGCCCTGCCGTCGGGTGCGTGCCGAATCTGCGTGCGCACCCGATTTCCGATCTTCCGCTTGACCCTCACGTAGCGTA
ACGTTCGAGACTCCAGTGTG

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 521; Mature: 521

Protein sequence:

>521_residues
MDRSMNLKTQAVRALAVASLAGPLAGCSWFAPSGEPPAMPSPAHYGTTPQVQQTVSAQGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGASRFANRALARRVDVSAFQLESARRALAANIVTASITVSVLNAQINTTERLVALANDQAHDAERRYALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDQPPADLTLADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPVEKADKAGKTDKATGDSATGQQARAATADTPPSPQ

Sequences:

>Translated_521_residues
MDRSMNLKTQAVRALAVASLAGPLAGCSWFAPSGEPPAMPSPAHYGTTPQVQQTVSAQGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGASRFANRALARRVDVSAFQLESARRALAANIVTASITVSVLNAQINTTERLVALANDQAHDAERRYALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDQPPADLTLADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPVEKADKAGKTDKATGDSATGQQARAATADTPPSPQ
>Mature_521_residues
MDRSMNLKTQAVRALAVASLAGPLAGCSWFAPSGEPPAMPSPAHYGTTPQVQQTVSAQGVAQQFEVGAQPVPDWWKQYRS
DALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSMLPSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASY
TIDLFGASRFANRALARRVDVSAFQLESARRALAANIVTASITVSVLNAQINTTERLVALANDQAHDAERRYALGSASRS
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDQPPADLTLADLHLPEQVPVVVPSDLLQSRPDIQAADAGLKA
AAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIWNVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLG
AFQNVADSLAALEHDAEALDASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
AMGTPPVEKADKAGKTDKATGDSATGQQARAATADTPPSPQ

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: COG1538

COG function: function code MU; Outer membrane protein

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=468, Percent_Identity=25.4273504273504, Blast_Score=99, Evalue=6e-22,
Organism=Escherichia coli, GI2367348, Length=387, Percent_Identity=23.5142118863049, Blast_Score=73, Evalue=4e-14,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 54409; Mature: 54409

Theoretical pI: Translated: 7.78; Mature: 7.78

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
1.7 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MDRSMNLKTQAVRALAVASLAGPLAGCSWFAPSGEPPAMPSPAHYGTTPQVQQTVSAQGV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AQQFEVGAQPVPDWWKQYRSDALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTIDLFGASRFANRALARRVD
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTASITVSVLNAQINTTERLVALANDQAHDAERRYALGSASRS
HHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCH
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDQPPADLTLADLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
CCHHHHHHHCCCCCHHHHHEEECCCCCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
AMGTPPVEKADKAGKTDKATGDSATGQQARAATADTPPSPQ
HHCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC
>Mature Secondary Structure
MDRSMNLKTQAVRALAVASLAGPLAGCSWFAPSGEPPAMPSPAHYGTTPQVQQTVSAQGV
CCCCCCHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHH
AQQFEVGAQPVPDWWKQYRSDALNALVDEGLRNSPTLSAASHSLDAAREQLRGQIGSSML
HHHHHCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCC
PSIDAGGQATRQRALGVPIPALGAPTLLYDTFVGQLQASYTIDLFGASRFANRALARRVD
CCCCCCCHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHC
VSAFQLESARRALAANIVTASITVSVLNAQINTTERLVALANDQAHDAERRYALGSASRS
HHHHHHHHHHHHHHHHHHHHHEEEEEHHCCCCCHHHHHHHCCCCHHHHHHHHHCCCCCCH
DALNARQSADTFAASLPALRQQRDSARHALAVLVGRTPDQPPADLTLADLHLPEQVPVVV
HHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCEEEECCCCCCCCEEC
PSDLLQSRPDIQAADAGLKAAAAEVGLATAQMFPQLSLSAAMGKGGFSWPTMLSGAGSIW
CHHHHCCCCCCHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCHH
NVGASLSQPIFHGGALLAQRRAAKATYEAAVDQYKQAVLGAFQNVADSLAALEHDAEALD
CCCCHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
ASSRAALSARGAYDDAAARVRLGALPPSAARASELQYRNARLDEIRATGARFADTARLYQ
CCHHHHHHHCCCCCHHHHHEEECCCCCCHHHHHHHHHHHCHHHHHHHCCCHHHHHHHHHH
AMGTPPVEKADKAGKTDKATGDSATGQQARAATADTPPSPQ
HHCCCCHHHHHCCCCCCCCCCCCCCCCHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]