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The map label for this gene is ynfJ [C]

Identifier: 78066502

GI number: 78066502

Start: 2067339

End: 2069135

Strand: Direct

Name: ynfJ [C]

Synonym: Bcep18194_A5033

Alternate gene names: 78066502

Gene position: 2067339-2069135 (Clockwise)

Preceding gene: 78066501

Following gene: 78066504

Centisome position: 55.96

GC content: 68.0

Gene sequence:

>1797_bases
ATGGTCCCGCGGGCGCGGCGCAGTGCTATGCTTCGCGTCGTTTTCCATCGCCCTCTTTCAGCCGTGCTCTCGTTCCTGCT
GAAGCTGCGCACTCGCGCGCAAACGCTGTTCCGCCTGTCGGACGCCCATACGATGCTGATCTGGTCGGCCATCGTCGGCG
TCGGCGGCGCCTTTGCGACGATGGCGTTCCGCGAAGGCATCGACCTGATGCAGCACCTGATCTCCGGGCAAAGCGGCAGC
TTCGTGCAGATGGCCAAGCGCCTGCCCTGGTACGTGCGGTTCTGGATGCCGGCCGCGGGCGGCTTCCTCGCCGGCTGCGT
GCTGCTGCTCGCGACGCGCGGCGACAAGAAGTCCGGAAAGACCGACTACATGGAATCGGTCGCGCTCGGCAACGGCGTCG
TGCCGGTTCGCCAGAGCCTGTGGCGCAGCGTGTCGTCGCTGCTGACGATCGGCAGCGGCGGCTCGATCGGGCGCGAAGGC
CCGATGGTGCAGCTCGCGGCGCTCGCCGCATCGCTCGTCGGCCGCTTCGCGCACTTCGACCCGCCGCGCCTGCGCCTGCT
GGTCGCCTGCGGGGCCGCCGCCGGCATCACGTCCGCGTACAACGCGCCGATCGCCGGCGCCTTCTTCGTGTCGGAGATCG
TGCTCGGCGGCATCGCCATGGAAAGTCTCGGGCCGATGATCGTCTCGTCGGTCGTCGCGAACATCGTGATGCGCGAATTT
GCCGGCTACCGGCCGCCGTACGAGATGCCGGTGTTTCCGGCCGTCACCGGCCCCGAAGTGCTGCTGTTCGTCGTGCTCGG
CGCGCTGTGCGGCGTGCTCGCGCCGCAATTCCTGCACCTGCTCGACGCATCGAAGAACAAATTCAAGCGGCTGCCCTTGC
CGCTGCCCGTGCGGCTCGCGCTCGGCGGCCTCGTCGTCGGCGTGATCTCGGTATGGGTGCCGGACGTGTGGGGCAACGGC
TACAGCGTGGTGAACCACATCCTCCACTCGCCGTGGACCTGGCAGGCACTCGTCGCGGTGCTCGTGTTCAAGGTGATCGC
GACCTCCGCCACCGTCGGCTCCGGCGCAATCGGCGGGGTGTTCACGCCGACGCTGTTCGTCGGCGCGGTGTTCGGCTCGC
TGTTCGGGCTCGCGATGCAAGCCGTGTGGCCCGGCCATACGTCGCCGTACTTCGCGTACGCGATCGTCGGGATGGGCGCG
TTCCTGACGGGCGCCACGCAGGCGCCGCTGATGGCGATCCTGATGATCTTCGAGATGACGCTCAGCTACCAGGTCGTGCT
GCCGCTGCTGGCGTCCTGCGTATTCGCGTACTTCGTCGCGCGCGCGACCGGCACGACGTCGATGTACGAGATCACGCTGC
ACCACCACCAGGATGCCGAGGAACGCAGCCGGATCCGCACCACGCAGATGCGCGAGCTGATCCAGCCCGCGCAGACGGTC
GTGCCGCTCACCGCGAGCGTCGCCGACATGACGCGCGTGTTTCTCGAATATCCGGTGAAGTACCTGTACGTGACCGACGA
CGCCGGGCGCTTTCGCGGCGCAGTCGCGCTGAAGGACATCACGTCGGACCTGCTCGACAAGCGCGACACGACCGACAAGA
CGGCTGCGCACTACGCGCACACGCCGTTTCCGCTCCTCACGCCCGACATGCCGCTCGCCACCGCGCTCGAACGCTTCATG
GCGTTCCAGGGCGAACGCCTGCCGGTGATCGAAAGCGAAGCCGAGCCGACGCTCGCGGGGGTCGTCTACAAGACGTCGCT
GCTCGATGCGTACCGGCGGATGACCGGCGAACGCTGA

Upstream 100 bases:

>100_bases
GTGTGGCTGATCGCCGGCGGTTACGGGATCACCTGGGCCGTGCAGCACTGGCTTGGCTGAGTTGCCCGCCCGTGACACTT
CGCGCGCCGCGCCCGACGGG

Downstream 100 bases:

>100_bases
CCGCACGCCTCGCCGCCCGCCGCGCGAGCGCGGCTCGAGGCCAACGCCCGCTCAGTCGGGGGCGCGCCCTAGCACCACGC
GCGCGAAGAATCCCGCGAGC

Product: putative voltage-gated ClC-type chloride channel ClcB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 598; Mature: 598

Protein sequence:

>598_residues
MVPRARRSAMLRVVFHRPLSAVLSFLLKLRTRAQTLFRLSDAHTMLIWSAIVGVGGAFATMAFREGIDLMQHLISGQSGS
FVQMAKRLPWYVRFWMPAAGGFLAGCVLLLATRGDKKSGKTDYMESVALGNGVVPVRQSLWRSVSSLLTIGSGGSIGREG
PMVQLAALAASLVGRFAHFDPPRLRLLVACGAAAGITSAYNAPIAGAFFVSEIVLGGIAMESLGPMIVSSVVANIVMREF
AGYRPPYEMPVFPAVTGPEVLLFVVLGALCGVLAPQFLHLLDASKNKFKRLPLPLPVRLALGGLVVGVISVWVPDVWGNG
YSVVNHILHSPWTWQALVAVLVFKVIATSATVGSGAIGGVFTPTLFVGAVFGSLFGLAMQAVWPGHTSPYFAYAIVGMGA
FLTGATQAPLMAILMIFEMTLSYQVVLPLLASCVFAYFVARATGTTSMYEITLHHHQDAEERSRIRTTQMRELIQPAQTV
VPLTASVADMTRVFLEYPVKYLYVTDDAGRFRGAVALKDITSDLLDKRDTTDKTAAHYAHTPFPLLTPDMPLATALERFM
AFQGERLPVIESEAEPTLAGVVYKTSLLDAYRRMTGER

Sequences:

>Translated_598_residues
MVPRARRSAMLRVVFHRPLSAVLSFLLKLRTRAQTLFRLSDAHTMLIWSAIVGVGGAFATMAFREGIDLMQHLISGQSGS
FVQMAKRLPWYVRFWMPAAGGFLAGCVLLLATRGDKKSGKTDYMESVALGNGVVPVRQSLWRSVSSLLTIGSGGSIGREG
PMVQLAALAASLVGRFAHFDPPRLRLLVACGAAAGITSAYNAPIAGAFFVSEIVLGGIAMESLGPMIVSSVVANIVMREF
AGYRPPYEMPVFPAVTGPEVLLFVVLGALCGVLAPQFLHLLDASKNKFKRLPLPLPVRLALGGLVVGVISVWVPDVWGNG
YSVVNHILHSPWTWQALVAVLVFKVIATSATVGSGAIGGVFTPTLFVGAVFGSLFGLAMQAVWPGHTSPYFAYAIVGMGA
FLTGATQAPLMAILMIFEMTLSYQVVLPLLASCVFAYFVARATGTTSMYEITLHHHQDAEERSRIRTTQMRELIQPAQTV
VPLTASVADMTRVFLEYPVKYLYVTDDAGRFRGAVALKDITSDLLDKRDTTDKTAAHYAHTPFPLLTPDMPLATALERFM
AFQGERLPVIESEAEPTLAGVVYKTSLLDAYRRMTGER
>Mature_598_residues
MVPRARRSAMLRVVFHRPLSAVLSFLLKLRTRAQTLFRLSDAHTMLIWSAIVGVGGAFATMAFREGIDLMQHLISGQSGS
FVQMAKRLPWYVRFWMPAAGGFLAGCVLLLATRGDKKSGKTDYMESVALGNGVVPVRQSLWRSVSSLLTIGSGGSIGREG
PMVQLAALAASLVGRFAHFDPPRLRLLVACGAAAGITSAYNAPIAGAFFVSEIVLGGIAMESLGPMIVSSVVANIVMREF
AGYRPPYEMPVFPAVTGPEVLLFVVLGALCGVLAPQFLHLLDASKNKFKRLPLPLPVRLALGGLVVGVISVWVPDVWGNG
YSVVNHILHSPWTWQALVAVLVFKVIATSATVGSGAIGGVFTPTLFVGAVFGSLFGLAMQAVWPGHTSPYFAYAIVGMGA
FLTGATQAPLMAILMIFEMTLSYQVVLPLLASCVFAYFVARATGTTSMYEITLHHHQDAEERSRIRTTQMRELIQPAQTV
VPLTASVADMTRVFLEYPVKYLYVTDDAGRFRGAVALKDITSDLLDKRDTTDKTAAHYAHTPFPLLTPDMPLATALERFM
AFQGERLPVIESEAEPTLAGVVYKTSLLDAYRRMTGER

Specific function: Unknown

COG id: COG0038

COG function: function code P; Chloride channel protein EriC

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the chloride channel (TC 2.A.49) family. ClcB subfamily [H]

Homologues:

Organism=Homo sapiens, GI55770838, Length=498, Percent_Identity=23.4939759036145, Blast_Score=77, Evalue=3e-14,
Organism=Homo sapiens, GI55770840, Length=498, Percent_Identity=23.4939759036145, Blast_Score=77, Evalue=4e-14,
Organism=Homo sapiens, GI153252026, Length=499, Percent_Identity=23.6472945891784, Blast_Score=74, Evalue=4e-13,
Organism=Escherichia coli, GI87081943, Length=422, Percent_Identity=38.3886255924171, Blast_Score=199, Evalue=6e-52,
Organism=Escherichia coli, GI1786350, Length=326, Percent_Identity=27.9141104294479, Blast_Score=99, Evalue=6e-22,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR014743
- InterPro:   IPR001807 [H]

Pfam domain/function: PF00654 Voltage_CLC [H]

EC number: NA

Molecular weight: Translated: 64660; Mature: 64660

Theoretical pI: Translated: 9.82; Mature: 9.82

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
4.0 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
4.0 %Met     (Mature Protein)
4.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVPRARRSAMLRVVFHRPLSAVLSFLLKLRTRAQTLFRLSDAHTMLIWSAIVGVGGAFAT
CCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHHHHHCCHHHHH
MAFREGIDLMQHLISGQSGSFVQMAKRLPWYVRFWMPAAGGFLAGCVLLLATRGDKKSGK
HHHHHHHHHHHHHHCCCCCCHHHHHHHCCCEEEEECCCCCHHHHHHHHHHHCCCCCCCCC
TDYMESVALGNGVVPVRQSLWRSVSSLLTIGSGGSIGREGPMVQLAALAASLVGRFAHFD
HHHHHHHHHCCCCHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHHHHHHHHHCCC
PPRLRLLVACGAAAGITSAYNAPIAGAFFVSEIVLGGIAMESLGPMIVSSVVANIVMREF
CHHHHHHHHHCCHHCCCHHCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AGYRPPYEMPVFPAVTGPEVLLFVVLGALCGVLAPQFLHLLDASKNKFKRLPLPLPVRLA
HCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHCCCCCCCHHHH
LGGLVVGVISVWVPDVWGNGYSVVNHILHSPWTWQALVAVLVFKVIATSATVGSGAIGGV
HHHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHCCCCCCCCCCH
FTPTLFVGAVFGSLFGLAMQAVWPGHTSPYFAYAIVGMGAFLTGATQAPLMAILMIFEMT
HHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHH
LSYQVVLPLLASCVFAYFVARATGTTSMYEITLHHHQDAEERSRIRTTQMRELIQPAQTV
HHHHHHHHHHHHHHHHHHHHHCCCCCEEEEEEEECCCCHHHHHHHHHHHHHHHHHHHHHH
VPLTASVADMTRVFLEYPVKYLYVTDDAGRFRGAVALKDITSDLLDKRDTTDKTAAHYAH
HHHHHHHHHHHHHHHHCCEEEEEEECCCCHHHHHHHHHHHHHHHHCCCCCCCHHHHHHCC
TPFPLLTPDMPLATALERFMAFQGERLPVIESEAEPTLAGVVYKTSLLDAYRRMTGER
CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC
>Mature Secondary Structure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CCCCCCCCCCCHHHHHHHHHHHCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11823852 [H]