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The map label for this gene is emrA [H]

Identifier: 78066436

GI number: 78066436

Start: 1992643

End: 1993674

Strand: Reverse

Name: emrA [H]

Synonym: Bcep18194_A4967

Alternate gene names: 78066436

Gene position: 1993674-1992643 (Counterclockwise)

Preceding gene: 78066438

Following gene: 78066435

Centisome position: 53.97

GC content: 61.82

Gene sequence:

>1032_bases
ATGGTGACGCAGACGCCCCAGGACACTCCATCGGAGCCGCCCGAGCGCGGTCGCCGGTTGCCGATCGTACTGATCGCCAT
GTTCGCGATTCTGCTCGTGACGGTACTGATTTACGAGTTCGAAGTTCGAGACCTCAGTACCGACGACGCTTATGTGACCG
GCCATCTGCATGTGATCTCTCCACGTGTCAGCGGTACCGTCGAGCGTGTTCTGGTGAACGACAATCAATTCGTGCACGCC
GGGGATCCGCTCACACTTCTTGATCCGCGAGATTTCGACGTACGCGTCGCATTGCAACGTTCGCGCGTTGCACAAGCGCA
GTCCGACGCCGCCCGTGCACGCGCACTCGTCGAACAGGCGGTCGCGACACGAATATCGGCGCAAGCCGATGCCGACAAGG
CAGAGCTCGATTACGCAAGAGCAAATGAGTTGACCCGCGAGACGCCGCGGGGGCTTTCCAAGCAGGAATACGATGCAGCT
GACGCCGCGCGCAAGTCTGCGCGCGCGAGGATCGTCGCGGCCGACGCGCAACTCCGTAGCACACGGGCCGCAGCGCAGGC
GGCAGATGCCGTATCGGGACAGAACGATGCAGAGCTGCGCGACGCGCTGCTTCAGCGCGAGTACACAACAGTCATCGCGC
CTTCGGACGGCTATGTTGGCAAGAAGACCGTGGAAACAGGCGAGCACGTCGCGCCCGGCCAGGCACTGCTCACGATCGTC
GAACCTCATCCATGGGTCGTCGCAAATTTCCGCGAGACCCAGCTTCGGCATGTTCGCGTGGGCGAGCCGGTACGACTTCA
CTTCGATGCGCTTCCGGACGTGGAGTTTGTCGGTCACATCGACAGCCAGTCACCGGCAACGGGGTCACAGTTTTCATTAT
TGCCACCGGACAACGCAACAGGCAATTTCACGAAGGTCACGCAGCGGGTGCCAGTCAAAATCCTGCTGGACGGCCGAGCG
GCAATCGAACCTCGCATCCACCCCGGGCTGTCGGTCGTCGTCACGCTGCAACGCGGACGTCATTCGCCATGA

Upstream 100 bases:

>100_bases
GCGACGAGCATGGTACTCATTGTCGAACAACTCCCGCGCAGGACGTTTGCGCAAGCATACCGCGACGTCCTGAATCCTGC
CTGCATGATGGAGAAAACAG

Downstream 100 bases:

>100_bases
AACGTGCATCCGCCCTGATCGCCGCGTACGCGCTGGCCTCTTGCACGATTGTCCCGCGGCCGCTCGTGCCGGTACCAGTC
GGACATGATACGTTCCGCCA

Product: HlyD family secretion protein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 343; Mature: 343

Protein sequence:

>343_residues
MVTQTPQDTPSEPPERGRRLPIVLIAMFAILLVTVLIYEFEVRDLSTDDAYVTGHLHVISPRVSGTVERVLVNDNQFVHA
GDPLTLLDPRDFDVRVALQRSRVAQAQSDAARARALVEQAVATRISAQADADKAELDYARANELTRETPRGLSKQEYDAA
DAARKSARARIVAADAQLRSTRAAAQAADAVSGQNDAELRDALLQREYTTVIAPSDGYVGKKTVETGEHVAPGQALLTIV
EPHPWVVANFRETQLRHVRVGEPVRLHFDALPDVEFVGHIDSQSPATGSQFSLLPPDNATGNFTKVTQRVPVKILLDGRA
AIEPRIHPGLSVVVTLQRGRHSP

Sequences:

>Translated_343_residues
MVTQTPQDTPSEPPERGRRLPIVLIAMFAILLVTVLIYEFEVRDLSTDDAYVTGHLHVISPRVSGTVERVLVNDNQFVHA
GDPLTLLDPRDFDVRVALQRSRVAQAQSDAARARALVEQAVATRISAQADADKAELDYARANELTRETPRGLSKQEYDAA
DAARKSARARIVAADAQLRSTRAAAQAADAVSGQNDAELRDALLQREYTTVIAPSDGYVGKKTVETGEHVAPGQALLTIV
EPHPWVVANFRETQLRHVRVGEPVRLHFDALPDVEFVGHIDSQSPATGSQFSLLPPDNATGNFTKVTQRVPVKILLDGRA
AIEPRIHPGLSVVVTLQRGRHSP
>Mature_343_residues
MVTQTPQDTPSEPPERGRRLPIVLIAMFAILLVTVLIYEFEVRDLSTDDAYVTGHLHVISPRVSGTVERVLVNDNQFVHA
GDPLTLLDPRDFDVRVALQRSRVAQAQSDAARARALVEQAVATRISAQADADKAELDYARANELTRETPRGLSKQEYDAA
DAARKSARARIVAADAQLRSTRAAAQAADAVSGQNDAELRDALLQREYTTVIAPSDGYVGKKTVETGEHVAPGQALLTIV
EPHPWVVANFRETQLRHVRVGEPVRLHFDALPDVEFVGHIDSQSPATGSQFSLLPPDNATGNFTKVTQRVPVKILLDGRA
AIEPRIHPGLSVVVTLQRGRHSP

Specific function: The emr locus confers resistance to substances of high hydrophobicity. EmrA probably participate in a transport system to extrude toxins and drugs from the cell [H]

COG id: COG1566

COG function: function code V; Multidrug resistance efflux pump

Gene ontology:

Cell location: Cell inner membrane; Single-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the membrane fusion protein (MFP) (TC 8.A.1) family [H]

Homologues:

Organism=Escherichia coli, GI1789041, Length=346, Percent_Identity=34.971098265896, Blast_Score=162, Evalue=3e-41,
Organism=Escherichia coli, GI1788711, Length=332, Percent_Identity=33.1325301204819, Blast_Score=148, Evalue=6e-37,
Organism=Escherichia coli, GI1790519, Length=296, Percent_Identity=31.0810810810811, Blast_Score=102, Evalue=5e-23,
Organism=Escherichia coli, GI87081951, Length=306, Percent_Identity=25.1633986928105, Blast_Score=89, Evalue=3e-19,
Organism=Escherichia coli, GI1790024, Length=241, Percent_Identity=26.5560165975104, Blast_Score=72, Evalue=4e-14,
Organism=Escherichia coli, GI1790012, Length=239, Percent_Identity=27.6150627615063, Blast_Score=65, Evalue=7e-12,
Organism=Escherichia coli, GI1787013, Length=240, Percent_Identity=32.0833333333333, Blast_Score=62, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005694
- InterPro:   IPR006143 [H]

Pfam domain/function: PF00529 HlyD [H]

EC number: NA

Molecular weight: Translated: 37446; Mature: 37446

Theoretical pI: Translated: 6.52; Mature: 6.52

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
0.6 %Met     (Translated Protein)
0.6 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MVTQTPQDTPSEPPERGRRLPIVLIAMFAILLVTVLIYEFEVRDLSTDDAYVTGHLHVIS
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEC
PRVSGTVERVLVNDNQFVHAGDPLTLLDPRDFDVRVALQRSRVAQAQSDAARARALVEQA
CCCCCCEEEEEECCCCEEECCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
VATRISAQADADKAELDYARANELTRETPRGLSKQEYDAADAARKSARARIVAADAQLRS
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCEEEEEECHHHHH
TRAAAQAADAVSGQNDAELRDALLQREYTTVIAPSDGYVGKKTVETGEHVAPGQALLTIV
HHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
EPHPWVVANFRETQLRHVRVGEPVRLHFDALPDVEFVGHIDSQSPATGSQFSLLPPDNAT
CCCCEEEECCCHHHEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCC
GNFTKVTQRVPVKILLDGRAAIEPRIHPGLSVVVTLQRGRHSP
CCHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC
>Mature Secondary Structure
MVTQTPQDTPSEPPERGRRLPIVLIAMFAILLVTVLIYEFEVRDLSTDDAYVTGHLHVIS
CCCCCCCCCCCCCHHHCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEEEEEEC
PRVSGTVERVLVNDNQFVHAGDPLTLLDPRDFDVRVALQRSRVAQAQSDAARARALVEQA
CCCCCCEEEEEECCCCEEECCCCEEEECCCCCCEEEEEHHHHHHHHHHHHHHHHHHHHHH
VATRISAQADADKAELDYARANELTRETPRGLSKQEYDAADAARKSARARIVAADAQLRS
HHHHHHCCCCCCHHHHHHHHHHHHHHHCCCCCCCHHCCHHHHHHHHHCEEEEEECHHHHH
TRAAAQAADAVSGQNDAELRDALLQREYTTVIAPSDGYVGKKTVETGEHVAPGQALLTIV
HHHHHHHHHHHCCCCHHHHHHHHHHCCCEEEEECCCCCCCCCCCCCCCCCCCCCEEEEEE
EPHPWVVANFRETQLRHVRVGEPVRLHFDALPDVEFVGHIDSQSPATGSQFSLLPPDNAT
CCCCEEEECCCHHHEEEEECCCCEEEEECCCCCCCEEECCCCCCCCCCCEEEEECCCCCC
GNFTKVTQRVPVKILLDGRAAIEPRIHPGLSVVVTLQRGRHSP
CCHHHHHHCCCEEEEECCCCCCCCCCCCCCEEEEEEECCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 1409590; 9205837; 9278503; 8203018 [H]