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The map label for this gene is oprM [H]

Identifier: 78066435

GI number: 78066435

Start: 1991246

End: 1992646

Strand: Reverse

Name: oprM [H]

Synonym: Bcep18194_A4966

Alternate gene names: 78066435

Gene position: 1992646-1991246 (Counterclockwise)

Preceding gene: 78066436

Following gene: 78066433

Centisome position: 53.94

GC content: 62.74

Gene sequence:

>1401_bases
ATGAAACGTGCATCCGCCCTGATCGCCGCGTACGCGCTGGCCTCTTGCACGATTGTCCCGCGGCCGCTCGTGCCGGTACC
AGTCGGACATGATACGTTCCGCCACGCTGGTACACCGCTTGCGCACGAGGCCGCGTTGACCGATTGGCCCGCGCTGTTCG
GCGACCCGCAACTCAGTGCACTCGTCGATGCCGCACTCACACACAACAATGACATCCGTGCCGCATCCGCGCGGATCGAT
CAAGCTCAGGCGCTGCTCGGCATGCACGAGGCCTCGCTGTTGCCGTCAGTTACCGTCGACCCCTCGTTCAGTCGCGCACG
TGTCTCTGGCACCGTTGACAACGCACTGCCGAAACGCATGATGCATAACTGGTCGGCCCCGGTGACAGCAAGCTACGAAA
TCGACCTGTGGGGACGCGTGCGCGGAGATGTCGACATCGAACGCGAGAATGTGCTGCAGGCAATCGCAGATCGAGACGCT
GTTCGTCTACGCGTCGCAACGGATGTCGCGAGCGACTACCTGACGCTGCATTATGTTGAACAGGATCTCAACGCACTTAC
TCGTGTGATCGACCTGAGATACACAGCGCTCGATGTGATCTCCGCACGCGTGCGAGCCGGCGCGGCGAGCGATCTCGATT
CTCTACGCGCAGCGGCCGATCTCGACACTGCGCGAGCCGACCTTGCGGACAGCCAGCGGACCCGTGAAAATCTCATCGAC
GCGCTCGCCATCCTGACCGGCGTATCGCCAACTGCGATCGCAATCGATCCGACCGCGGTGAACCTCCGTGTGCCGGATGT
GCCATCCGGCTTGCCGTCCGCGTTGCTCGGCCATCGACCAGATGTATTCGCAGCCGAGCGCCGTGTGGATGCGGCATCGC
TCGAGCTTGGCATTGCCCGCAAGGCCTGGCTGCCCACGCTGTCGCTCACCGCACAAGGTGGCTTTGCGAGTCGCGACCTG
CGTACATTCCTCGACCGTAACAGTTCGCTGTGGAGCTTCGGCCTGAGCATGGCCGAGACTATCTTCGATGGTGGCAAGCG
TGACGCGGCAGTGGCCGCCGCCCAGGCAGGGGTAACCGTTGCAGACGCGAACTATCGCTCAATCGCAATCGGCGCGCTAC
GCGAGGTACAGGATGCGCTCAACAACATTGCGTCCGAGAAGGAACGGATCGCACGCCTCGATAGCGCGGCACGTACTACC
GAGGCCGCAGCACGACTGTCAGTGAGCCGATACGAGCATGGCTACGTCAGTTACCTGGAGGTGATAGACGCCGATCGGGA
CGCTCTCAATGCGCAGCGCCAGTGGATCCATAGCCGCCAGGCCTACTCGGTCGCGACGGTAAGCCTTGTGCGTGCACTGG
GTGGCGGATGGATACCGCCAGTCCCAGCAGACGCACGATGA

Upstream 100 bases:

>100_bases
TGCCAGTCAAAATCCTGCTGGACGGCCGAGCGGCAATCGAACCTCGCATCCACCCCGGGCTGTCGGTCGTCGTCACGCTG
CAACGCGGACGTCATTCGCC

Downstream 100 bases:

>100_bases
CAGACTCAAAAAATTAAGGAATTTTCAGGATTTTTAATATGTCACGTCACTGCGAACCGGGGCGTGCCGAAAACCGCTTG
CCGGGCAGAATCAGCACATT

Product: RND efflux system outer membrane lipoprotein

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 466; Mature: 466

Protein sequence:

>466_residues
MKRASALIAAYALASCTIVPRPLVPVPVGHDTFRHAGTPLAHEAALTDWPALFGDPQLSALVDAALTHNNDIRAASARID
QAQALLGMHEASLLPSVTVDPSFSRARVSGTVDNALPKRMMHNWSAPVTASYEIDLWGRVRGDVDIERENVLQAIADRDA
VRLRVATDVASDYLTLHYVEQDLNALTRVIDLRYTALDVISARVRAGAASDLDSLRAAADLDTARADLADSQRTRENLID
ALAILTGVSPTAIAIDPTAVNLRVPDVPSGLPSALLGHRPDVFAAERRVDAASLELGIARKAWLPTLSLTAQGGFASRDL
RTFLDRNSSLWSFGLSMAETIFDGGKRDAAVAAAQAGVTVADANYRSIAIGALREVQDALNNIASEKERIARLDSAARTT
EAAARLSVSRYEHGYVSYLEVIDADRDALNAQRQWIHSRQAYSVATVSLVRALGGGWIPPVPADAR

Sequences:

>Translated_466_residues
MKRASALIAAYALASCTIVPRPLVPVPVGHDTFRHAGTPLAHEAALTDWPALFGDPQLSALVDAALTHNNDIRAASARID
QAQALLGMHEASLLPSVTVDPSFSRARVSGTVDNALPKRMMHNWSAPVTASYEIDLWGRVRGDVDIERENVLQAIADRDA
VRLRVATDVASDYLTLHYVEQDLNALTRVIDLRYTALDVISARVRAGAASDLDSLRAAADLDTARADLADSQRTRENLID
ALAILTGVSPTAIAIDPTAVNLRVPDVPSGLPSALLGHRPDVFAAERRVDAASLELGIARKAWLPTLSLTAQGGFASRDL
RTFLDRNSSLWSFGLSMAETIFDGGKRDAAVAAAQAGVTVADANYRSIAIGALREVQDALNNIASEKERIARLDSAARTT
EAAARLSVSRYEHGYVSYLEVIDADRDALNAQRQWIHSRQAYSVATVSLVRALGGGWIPPVPADAR
>Mature_466_residues
MKRASALIAAYALASCTIVPRPLVPVPVGHDTFRHAGTPLAHEAALTDWPALFGDPQLSALVDAALTHNNDIRAASARID
QAQALLGMHEASLLPSVTVDPSFSRARVSGTVDNALPKRMMHNWSAPVTASYEIDLWGRVRGDVDIERENVLQAIADRDA
VRLRVATDVASDYLTLHYVEQDLNALTRVIDLRYTALDVISARVRAGAASDLDSLRAAADLDTARADLADSQRTRENLID
ALAILTGVSPTAIAIDPTAVNLRVPDVPSGLPSALLGHRPDVFAAERRVDAASLELGIARKAWLPTLSLTAQGGFASRDL
RTFLDRNSSLWSFGLSMAETIFDGGKRDAAVAAAQAGVTVADANYRSIAIGALREVQDALNNIASEKERIARLDSAARTT
EAAARLSVSRYEHGYVSYLEVIDADRDALNAQRQWIHSRQAYSVATVSLVRALGGGWIPPVPADAR

Specific function: The ability to export antibiotics and solvents is dramatically decreased in the presence of the proton conductor carbonyl cyanide m-chlorophenylhydrazone (CCCP), showing that an energized inner membrane is required for efflux. It is thought that the mexB

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell outer membrane; Lipid-anchor. Note=Attached to the outer membrane by a lipid anchor and via the transmembrane beta-barrel. The membrane anchor is not necessary for antibiotic efflux. In one report (PubMed:10889211) the Cys-18- Gly mutant is reported

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the outer membrane factor (OMF) (TC 1.B.17) family [H]

Homologues:

Organism=Escherichia coli, GI1786785, Length=461, Percent_Identity=27.9826464208243, Blast_Score=165, Evalue=6e-42,
Organism=Escherichia coli, GI2367348, Length=492, Percent_Identity=25, Blast_Score=94, Evalue=1e-20,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003423
- InterPro:   IPR010131 [H]

Pfam domain/function: PF02321 OEP [H]

EC number: NA

Molecular weight: Translated: 49993; Mature: 49993

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: PS00013 PROKAR_LIPOPROTEIN

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.1 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
1.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRASALIAAYALASCTIVPRPLVPVPVGHDTFRHAGTPLAHEAALTDWPALFGDPQLSA
CCHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHCCCCCHHHHHHCCCCHHCCCCHHHH
LVDAALTHNNDIRAASARIDQAQALLGMHEASLLPSVTVDPSFSRARVSGTVDNALPKRM
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHEECCCHHHHHHHHH
MHNWSAPVTASYEIDLWGRVRGDVDIERENVLQAIADRDAVRLRVATDVASDYLTLHYVE
HHCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
QDLNALTRVIDLRYTALDVISARVRAGAASDLDSLRAAADLDTARADLADSQRTRENLID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAILTGVSPTAIAIDPTAVNLRVPDVPSGLPSALLGHRPDVFAAERRVDAASLELGIAR
HHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHH
KAWLPTLSLTAQGGFASRDLRTFLDRNSSLWSFGLSMAETIFDGGKRDAAVAAAQAGVTV
HHHCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEE
ADANYRSIAIGALREVQDALNNIASEKERIARLDSAARTTEAAARLSVSRYEHGYVSYLE
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VIDADRDALNAQRQWIHSRQAYSVATVSLVRALGGGWIPPVPADAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC
>Mature Secondary Structure
MKRASALIAAYALASCTIVPRPLVPVPVGHDTFRHAGTPLAHEAALTDWPALFGDPQLSA
CCHHHHHHHHHHHHHCEECCCCCCCCCCCCHHHHHCCCCCHHHHHHCCCCHHCCCCHHHH
LVDAALTHNNDIRAASARIDQAQALLGMHEASLLPSVTVDPSFSRARVSGTVDNALPKRM
HHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHCCCEECCCCCCHHEECCCHHHHHHHHH
MHNWSAPVTASYEIDLWGRVRGDVDIERENVLQAIADRDAVRLRVATDVASDYLTLHYVE
HHCCCCCEEEEEEEEEEEEECCCCCCCHHHHHHHHHCCCCEEEEEEHHHHHHHHHHHHHH
QDLNALTRVIDLRYTALDVISARVRAGAASDLDSLRAAADLDTARADLADSQRTRENLID
HHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
ALAILTGVSPTAIAIDPTAVNLRVPDVPSGLPSALLGHRPDVFAAERRVDAASLELGIAR
HHHHHHCCCCCEEEECCCEEEEECCCCCCCCCHHHHCCCCCHHHHHHCCCHHHHHHHHHH
KAWLPTLSLTAQGGFASRDLRTFLDRNSSLWSFGLSMAETIFDGGKRDAAVAAAQAGVTV
HHHCCEEEEEECCCCCHHHHHHHHCCCCCHHHHHHHHHHHHHCCCCCHHHHHHHHCCCEE
ADANYRSIAIGALREVQDALNNIASEKERIARLDSAARTTEAAARLSVSRYEHGYVSYLE
ECCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHH
VIDADRDALNAQRQWIHSRQAYSVATVSLVRALGGGWIPPVPADAR
HHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 8226684; 10889211; 10984043; 7968531; 8540696; 9401051; 9603892; 10952562 [H]