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The map label for this gene is prfH

Identifier: 78066331

GI number: 78066331

Start: 1879275

End: 1879889

Strand: Reverse

Name: prfH

Synonym: Bcep18194_A4861

Alternate gene names: 78066331

Gene position: 1879889-1879275 (Counterclockwise)

Preceding gene: 161702960

Following gene: 78066330

Centisome position: 50.89

GC content: 70.08

Gene sequence:

>615_bases
ATGCTGATGCAGATTTCTTCGGCGCACGGCCCGCTCGAATGCCAGCTGGCCGCGGCCAATGCGTTGCGGCGCCTGCAGGC
GGAAGCCGATGCGCAGCGTGTCGTCGTCACGGTGCTCGATGCGGAGCCGGGCGAACGGCCCGGTACGCTGCGCTCGGCGC
TGCTCGATCTCGACGGCGCCGGCGCGCAGGCGCTCGCGGACCGCTGGACCGGCACGCTGCAATGGATTTGCGCGAGCCCG
TACCGGTTGCGCCATCCGCGCAAGAACTGGTTCATCGGCGTCACGCGCTGTGCCGACGCGCAGCCGCTGCCGGATGGCGA
CGTGCGGTTCGAAGCGATGCGCGCACGCGGGCCGGGCGGCCAGCACGTGAACAAGACGAGCTCTGCGATTCGTGCGACCC
ATGTCGCGACCGGCCTGTCGGTGCGCGTGGAAAGCGAGCGCAGCCAGCACGCGAACAAGCGCCTGGCGCTGCAGTTGCTG
CAGGTTCGACTGCAGCAGGAAGCCGACCGTCACGCATCGGATGCGCGCCGGCAGCGGCGGATGCAGCACTTTGCGCTGGA
GCGCGGCAACCCCGTGCGCGTGTTTCACGGGGCGGCGTTCGTGCCGGCTGATTGA

Upstream 100 bases:

>100_bases
TCGACAGCGTCGTCGACGCGCTCGAGGCGGCCGGCCTGCTGCGCAAGCTGGCTCGGCTCGCGCCGGTGCTGACCTACAAG
ACGTCCGGGGAGGCTGGCCG

Downstream 100 bases:

>100_bases
GCACGAACGCGCGGCCGCCGGCGGTGGTTGCGCCGGCGGTCACGCAAGCGGCCTCATGGTTTTCCCGGCCTGCCGGTTGC
GCAATCGCGCAACCGGTTCC

Product: peptide chain release factor-like protein

Products: NA

Alternate protein names: RF-H [H]

Number of amino acids: Translated: 204; Mature: 204

Protein sequence:

>204_residues
MLMQISSAHGPLECQLAAANALRRLQAEADAQRVVVTVLDAEPGERPGTLRSALLDLDGAGAQALADRWTGTLQWICASP
YRLRHPRKNWFIGVTRCADAQPLPDGDVRFEAMRARGPGGQHVNKTSSAIRATHVATGLSVRVESERSQHANKRLALQLL
QVRLQQEADRHASDARRQRRMQHFALERGNPVRVFHGAAFVPAD

Sequences:

>Translated_204_residues
MLMQISSAHGPLECQLAAANALRRLQAEADAQRVVVTVLDAEPGERPGTLRSALLDLDGAGAQALADRWTGTLQWICASP
YRLRHPRKNWFIGVTRCADAQPLPDGDVRFEAMRARGPGGQHVNKTSSAIRATHVATGLSVRVESERSQHANKRLALQLL
QVRLQQEADRHASDARRQRRMQHFALERGNPVRVFHGAAFVPAD
>Mature_204_residues
MLMQISSAHGPLECQLAAANALRRLQAEADAQRVVVTVLDAEPGERPGTLRSALLDLDGAGAQALADRWTGTLQWICASP
YRLRHPRKNWFIGVTRCADAQPLPDGDVRFEAMRARGPGGQHVNKTSSAIRATHVATGLSVRVESERSQHANKRLALQLL
QVRLQQEADRHASDARRQRRMQHFALERGNPVRVFHGAAFVPAD

Specific function: Could be involved in translational termination [H]

COG id: COG1186

COG function: function code J; Protein chain release factor B

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the prokaryotic/mitochondrial release factor family [H]

Homologues:

Organism=Homo sapiens, GI34577120, Length=66, Percent_Identity=50, Blast_Score=70, Evalue=1e-12,
Organism=Escherichia coli, GI1787462, Length=187, Percent_Identity=31.0160427807487, Blast_Score=75, Evalue=3e-15,
Organism=Escherichia coli, GI2367172, Length=170, Percent_Identity=28.2352941176471, Blast_Score=68, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI17542784, Length=89, Percent_Identity=38.2022471910112, Blast_Score=66, Evalue=1e-11,
Organism=Saccharomyces cerevisiae, GI6321295, Length=59, Percent_Identity=52.5423728813559, Blast_Score=70, Evalue=2e-13,
Organism=Drosophila melanogaster, GI19921226, Length=105, Percent_Identity=39.0476190476191, Blast_Score=74, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000352
- InterPro:   IPR017509 [H]

Pfam domain/function: PF00472 RF-1 [H]

EC number: NA

Molecular weight: Translated: 22487; Mature: 22487

Theoretical pI: Translated: 11.14; Mature: 11.14

Prosite motif: PS00745 RF_PROK_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.5 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.5 %Cys     (Mature Protein)
2.0 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MLMQISSAHGPLECQLAAANALRRLQAEADAQRVVVTVLDAEPGERPGTLRSALLDLDGA
CEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCC
GAQALADRWTGTLQWICASPYRLRHPRKNWFIGVTRCADAQPLPDGDVRFEAMRARGPGG
CHHHHHHHHCCCEEEEECCCHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCC
QHVNKTSSAIRATHVATGLSVRVESERSQHANKRLALQLLQVRLQQEADRHASDARRQRR
CCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQHFALERGNPVRVFHGAAFVPAD
HHHHHHCCCCEEEEEECEEECCCC
>Mature Secondary Structure
MLMQISSAHGPLECQLAAANALRRLQAEADAQRVVVTVLDAEPGERPGTLRSALLDLDGA
CEEEECCCCCCEEEEEHHHHHHHHHHHHCCCCEEEEEEEECCCCCCCCHHHHHHHCCCCC
GAQALADRWTGTLQWICASPYRLRHPRKNWFIGVTRCADAQPLPDGDVRFEAMRARGPGG
CHHHHHHHHCCCEEEEECCCHHHCCCCCCEEEEEEECCCCCCCCCCCCEEEEEECCCCCC
QHVNKTSSAIRATHVATGLSVRVESERSQHANKRLALQLLQVRLQQEADRHASDARRQRR
CCCCHHHHHHHHHHHHCCEEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
MQHFALERGNPVRVFHGAAFVPAD
HHHHHHCCCCEEEEEECEEECCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 1695895; 9278503; 1408743 [H]