Click here to switch to the map view.

The map label for this gene is potF [H]

Identifier: 78066228

GI number: 78066228

Start: 1753932

End: 1755044

Strand: Direct

Name: potF [H]

Synonym: Bcep18194_A4758

Alternate gene names: 78066228

Gene position: 1753932-1755044 (Clockwise)

Preceding gene: 78066227

Following gene: 78066229

Centisome position: 47.48

GC content: 63.97

Gene sequence:

>1113_bases
ATGAAACGGAAAGGATGGTTGCGCTTCGCGGTGCCGGGCCTCGCGCTGTGCATGCTGGCCGGCATGGCCCGCGCCGATGA
AGAGAAGGTGCTGAACCTGTACAACTGGAGCAATTACTTCGCGCCGGATACGATTGCCGGCTTCGAAAAGGAAACCGGCA
TCAAGGTGCGCTACGACGCGTACGACAGCGACGAGACGCTGCAGTCGAAGCTGCTGACCGGCAACAGCGGCTACGACCTC
GTGTGGCCGACCAACGACTTCATGGCCAAGCAGATCCAGGCCGGCGTGTTTCGCAAGCTCGACAAGAACCGGCTGCCGAA
CCTGAAGTATCTCGATGGGTCGCTGCTGAAGCAGATGGCGCAATCCGATCCGGGCAACCAGTACGGCGTGCCGTACATGT
GGGGCACGGTGGGCGTCGGCTACGACCGCGCGAAGGTGCGTGCGATCCTCGGCAAGGACATGCCGGCCGACAGCCTCGAC
CTGCTGTTCAAGCCGGACATCGCCGCGCGCGTCGCCGCGAAGTGCGGGCTGGGCCTGCAGGATTCCGCGAGCACCGTGCT
GCCGCTGGCGTTGCGCTATATCGGCCGCGATCCGATGCATCCGTCCGCGCAGGACTATGCGGCCGCGCAGGCGATGCTGA
TGAAGATCCGGCCGTTCATCCGCGAGTTCGTCAATTCGTCCGATGCCAACGAGCTGATCGACGGCGAGCTGTGCGTGACG
ATCGGCTTCTCCGGCGCGATCAACCTGGCCGCGCAGAAGGCGAAGGCGCGCGATCCGAAACGCGACGTCGTGTACGACAT
TCCGCGGATCGGCACCCTGATGTGGTTCGACGCGATGGTCATTCCGGCCTCCTCGAAACATCCGGACAACGCGCATGCGT
TCATCAACTACATCCTGCGTCCGGACGTGATCGCGAAGATCTCGAATTCGACGCGCTACGCGAATGCGAACCAGGGCGCG
CTGAAGCTGATGGATCCGGCGCTGGTGCGCGATCCGGGCATCTATCCGGACGAGGCGACGCGGCGTACGCTGTTCACGCC
GATCGCCGAATCGCCGGCCGCGATGCGTATTGAAGGCCGGACCTGGCTCGGCTTCAAGACGGCGCGACCTTGA

Upstream 100 bases:

>100_bases
CGGCAATCGTGGGGCTTTTTCCGCGATCGCCGCCCGGAGATGTATCGCACGTTGCTGACCAGCGACGGCGTATCGTCCTG
TTATCGATGAGGAAGGCCCG

Downstream 100 bases:

>100_bases
CCGCGCGTCACGAAGCCACCGAACGATTGAAACGACCGAAGCACACGAAGTAACCGGCACGAGAAAGAGGAATGCCATGA
CAACGAGACGTCAACTGTTG

Product: ABC spermidine/putrescine transporter, periplasmic ligand binding protein

Products: ADP; phosphate; putrescine [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 370; Mature: 370

Protein sequence:

>370_residues
MKRKGWLRFAVPGLALCMLAGMARADEEKVLNLYNWSNYFAPDTIAGFEKETGIKVRYDAYDSDETLQSKLLTGNSGYDL
VWPTNDFMAKQIQAGVFRKLDKNRLPNLKYLDGSLLKQMAQSDPGNQYGVPYMWGTVGVGYDRAKVRAILGKDMPADSLD
LLFKPDIAARVAAKCGLGLQDSASTVLPLALRYIGRDPMHPSAQDYAAAQAMLMKIRPFIREFVNSSDANELIDGELCVT
IGFSGAINLAAQKAKARDPKRDVVYDIPRIGTLMWFDAMVIPASSKHPDNAHAFINYILRPDVIAKISNSTRYANANQGA
LKLMDPALVRDPGIYPDEATRRTLFTPIAESPAAMRIEGRTWLGFKTARP

Sequences:

>Translated_370_residues
MKRKGWLRFAVPGLALCMLAGMARADEEKVLNLYNWSNYFAPDTIAGFEKETGIKVRYDAYDSDETLQSKLLTGNSGYDL
VWPTNDFMAKQIQAGVFRKLDKNRLPNLKYLDGSLLKQMAQSDPGNQYGVPYMWGTVGVGYDRAKVRAILGKDMPADSLD
LLFKPDIAARVAAKCGLGLQDSASTVLPLALRYIGRDPMHPSAQDYAAAQAMLMKIRPFIREFVNSSDANELIDGELCVT
IGFSGAINLAAQKAKARDPKRDVVYDIPRIGTLMWFDAMVIPASSKHPDNAHAFINYILRPDVIAKISNSTRYANANQGA
LKLMDPALVRDPGIYPDEATRRTLFTPIAESPAAMRIEGRTWLGFKTARP
>Mature_370_residues
MKRKGWLRFAVPGLALCMLAGMARADEEKVLNLYNWSNYFAPDTIAGFEKETGIKVRYDAYDSDETLQSKLLTGNSGYDL
VWPTNDFMAKQIQAGVFRKLDKNRLPNLKYLDGSLLKQMAQSDPGNQYGVPYMWGTVGVGYDRAKVRAILGKDMPADSLD
LLFKPDIAARVAAKCGLGLQDSASTVLPLALRYIGRDPMHPSAQDYAAAQAMLMKIRPFIREFVNSSDANELIDGELCVT
IGFSGAINLAAQKAKARDPKRDVVYDIPRIGTLMWFDAMVIPASSKHPDNAHAFINYILRPDVIAKISNSTRYANANQGA
LKLMDPALVRDPGIYPDEATRRTLFTPIAESPAAMRIEGRTWLGFKTARP

Specific function: Required for the activity of the bacterial periplasmic transport system of putrescine. Polyamine binding protein [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Periplasm [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the bacterial solute-binding protein potD/potF family [H]

Homologues:

Organism=Escherichia coli, GI1787078, Length=368, Percent_Identity=43.2065217391304, Blast_Score=324, Evalue=6e-90,
Organism=Escherichia coli, GI1787367, Length=343, Percent_Identity=33.2361516034985, Blast_Score=174, Evalue=9e-45,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR006059
- InterPro:   IPR001188 [H]

Pfam domain/function: PF01547 SBP_bac_1 [H]

EC number: NA

Molecular weight: Translated: 40978; Mature: 40978

Theoretical pI: Translated: 9.41; Mature: 9.41

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
3.8 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
3.8 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKRKGWLRFAVPGLALCMLAGMARADEEKVLNLYNWSNYFAPDTIAGFEKETGIKVRYDA
CCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCEEEEECC
YDSDETLQSKLLTGNSGYDLVWPTNDFMAKQIQAGVFRKLDKNRLPNLKYLDGSLLKQMA
CCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHH
QSDPGNQYGVPYMWGTVGVGYDRAKVRAILGKDMPADSLDLLFKPDIAARVAAKCGLGLQ
HCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCC
DSASTVLPLALRYIGRDPMHPSAQDYAAAQAMLMKIRPFIREFVNSSDANELIDGELCVT
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEE
IGFSGAINLAAQKAKARDPKRDVVYDIPRIGTLMWFDAMVIPASSKHPDNAHAFINYILR
EECCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHC
PDVIAKISNSTRYANANQGALKLMDPALVRDPGIYPDEATRRTLFTPIAESPAAMRIEGR
CHHHEEECCCCEECCCCCCCEEECCCHHHCCCCCCCCCCCCCEEECCCCCCCCEEEECCC
TWLGFKTARP
EEECEECCCC
>Mature Secondary Structure
MKRKGWLRFAVPGLALCMLAGMARADEEKVLNLYNWSNYFAPDTIAGFEKETGIKVRYDA
CCCCCCEEEHHHHHHHHHHHHHCCCCHHHHHHHHCCCCCCCCCHHCCCCCCCCEEEEECC
YDSDETLQSKLLTGNSGYDLVWPTNDFMAKQIQAGVFRKLDKNRLPNLKYLDGSLLKQMA
CCCHHHHHHHHHCCCCCCEEEECCHHHHHHHHHHHHHHHHHHHCCCCCEECCHHHHHHHH
QSDPGNQYGVPYMWGTVGVGYDRAKVRAILGKDMPADSLDLLFKPDIAARVAAKCGLGLQ
HCCCCCCCCCCEEECCCCCCCCHHHHHHHHCCCCCCCCCCEEECCCHHHHHHHHHCCCCC
DSASTVLPLALRYIGRDPMHPSAQDYAAAQAMLMKIRPFIREFVNSSDANELIDGELCVT
CCCHHHHHHHHHHHCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCHHHCCCCEEEE
IGFSGAINLAAQKAKARDPKRDVVYDIPRIGTLMWFDAMVIPASSKHPDNAHAFINYILR
EECCCHHHHHHHHHHCCCCCCCCEECCCHHHHHHHHHEEEECCCCCCCCCHHHHHHHHHC
PDVIAKISNSTRYANANQGALKLMDPALVRDPGIYPDEATRRTLFTPIAESPAAMRIEGR
CHHHEEECCCCEECCCCCCCEEECCCHHHCCCCCCCCCCCCCEEECCCCCCCCEEEECCC
TWLGFKTARP
EEECEECCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: ATP; putrescine [Periplasm]; H2O [C]

Specific reaction: ATP + putrescine [Periplasm] + H2O = ADP + phosphate + putrescine [Cytoplasm] [C]

General reaction: Polyamine Binding Protein [C]

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 8416922; 8905232; 9278503; 9298646; 9651355 [H]