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The map label for this gene is 78066227

Identifier: 78066227

GI number: 78066227

Start: 1753007

End: 1753921

Strand: Direct

Name: 78066227

Synonym: Bcep18194_A4757

Alternate gene names: NA

Gene position: 1753007-1753921 (Clockwise)

Preceding gene: 78066226

Following gene: 78066228

Centisome position: 47.45

GC content: 65.9

Gene sequence:

>915_bases
ATGCCGTCCAAACACATCACCGTCGCCGCCGTCCAGATGGCGTCGGGCAACTGGAATCTCGAAGACAACATGGCCACCGC
GGAGCGCCTGATCCGCGCGGCCGCCGCACAGGGCGCGAATCTCGTGCTGTGCCCGGAGCTGTTCGCGATGCCGTACTTCT
GCCTCGACCAGAACGTGCGCCATCTCGAACTTGCGCAGCCGTTCGAAGGCAATGCGCAGATCGCCCGTTTCGCGGCGCTG
GCCGGCGAACTCGGCATCGTGCTGCCGATCGGCTTCTTCGAGCGCGCGGGCAATGCCGCGTACAACTCGATCGCCGTCGC
GGACGCGGACGGGCGCGTGCTCGGCGTCTATCGCAAGACGCATATCCCGGATGGGCCGGGCTATACGGAGAAGTTCTATT
TCACGCCGGGCGACACCGGCTTCAAGGTGTGGGATACGCGCTTCGGCCGAATCGGCATCGGCATCTGCTGGGACCAGTGG
TATCCGGAAACCGCACGCAGCCTCGCGCTGATGGGCGCCGAGATCCTGTGCTTTCCGACCATCATCGGCTCGGAGCCGTT
CAGCAGTGCGTTCGATTCGTCCGGCCACTGGCAGCGCACGATGCAGGGCCACGCGGCCGCCAACATGGTGCCGGTGGTGG
CCGCGAACCGGATCGGCCGCGAAGTCGGCTTCGGCAACGGCAATCCGGAGCAGCAGGGGCTCACGGGCGTGTTCTACGGG
TCGAGCTTCATCGCGGATCACACCGGCGAGAAGCGGGCCGAGGCGAACCGCACCGACGAAGCCGTGCTCGTGCACACGTT
CGATCTCGACGCGATCCGCGCCGACCGGCAATCGTGGGGCTTTTTCCGCGATCGCCGCCCGGAGATGTATCGCACGTTGC
TGACCAGCGACGGCGTATCGTCCTGTTATCGATGA

Upstream 100 bases:

>100_bases
GGCGGCGGCAGCATCCACTGTTCGACGCAGCAACAGCCGTCCGTCGCGAACTGAGCGACGTTTCGTCAGCGGTTTCCGGT
CCTACCGAGGAGCAATCGCA

Downstream 100 bases:

>100_bases
GGAAGGCCCGATGAAACGGAAAGGATGGTTGCGCTTCGCGGTGCCGGGCCTCGCGCTGTGCATGCTGGCCGGCATGGCCC
GCGCCGATGAAGAGAAGGTG

Product: Nitrilase/cyanide hydratase and apolipoprotein N-acyltransferase

Products: NA

Alternate protein names: D-N-alpha-carbamilase [H]

Number of amino acids: Translated: 304; Mature: 303

Protein sequence:

>304_residues
MPSKHITVAAVQMASGNWNLEDNMATAERLIRAAAAQGANLVLCPELFAMPYFCLDQNVRHLELAQPFEGNAQIARFAAL
AGELGIVLPIGFFERAGNAAYNSIAVADADGRVLGVYRKTHIPDGPGYTEKFYFTPGDTGFKVWDTRFGRIGIGICWDQW
YPETARSLALMGAEILCFPTIIGSEPFSSAFDSSGHWQRTMQGHAAANMVPVVAANRIGREVGFGNGNPEQQGLTGVFYG
SSFIADHTGEKRAEANRTDEAVLVHTFDLDAIRADRQSWGFFRDRRPEMYRTLLTSDGVSSCYR

Sequences:

>Translated_304_residues
MPSKHITVAAVQMASGNWNLEDNMATAERLIRAAAAQGANLVLCPELFAMPYFCLDQNVRHLELAQPFEGNAQIARFAAL
AGELGIVLPIGFFERAGNAAYNSIAVADADGRVLGVYRKTHIPDGPGYTEKFYFTPGDTGFKVWDTRFGRIGIGICWDQW
YPETARSLALMGAEILCFPTIIGSEPFSSAFDSSGHWQRTMQGHAAANMVPVVAANRIGREVGFGNGNPEQQGLTGVFYG
SSFIADHTGEKRAEANRTDEAVLVHTFDLDAIRADRQSWGFFRDRRPEMYRTLLTSDGVSSCYR
>Mature_303_residues
PSKHITVAAVQMASGNWNLEDNMATAERLIRAAAAQGANLVLCPELFAMPYFCLDQNVRHLELAQPFEGNAQIARFAALA
GELGIVLPIGFFERAGNAAYNSIAVADADGRVLGVYRKTHIPDGPGYTEKFYFTPGDTGFKVWDTRFGRIGIGICWDQWY
PETARSLALMGAEILCFPTIIGSEPFSSAFDSSGHWQRTMQGHAAANMVPVVAANRIGREVGFGNGNPEQQGLTGVFYGS
SFIADHTGEKRAEANRTDEAVLVHTFDLDAIRADRQSWGFFRDRRPEMYRTLLTSDGVSSCYR

Specific function: The enzyme catalyzes the hydrolysis of N-carbamoyl-D- amino acids to the corresponding which are useful intermediates in the preparation of beta-lactam antibiotics. Industrial production of beta-lactam antibiotics is now being developed using this enzyme

COG id: COG0388

COG function: function code R; Predicted amidohydrolase

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: NA

Operon status: Not Known

Operon components: None

Similarity: Contains 1 CN hydrolase domain [H]

Homologues:

Organism=Homo sapiens, GI5031947, Length=303, Percent_Identity=29.042904290429, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI297632348, Length=303, Percent_Identity=29.042904290429, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI297632350, Length=303, Percent_Identity=29.042904290429, Blast_Score=108, Evalue=8e-24,
Organism=Homo sapiens, GI7706509, Length=284, Percent_Identity=29.5774647887324, Blast_Score=107, Evalue=1e-23,
Organism=Homo sapiens, GI9910460, Length=300, Percent_Identity=29.6666666666667, Blast_Score=103, Evalue=1e-22,
Organism=Homo sapiens, GI297632346, Length=208, Percent_Identity=30.2884615384615, Blast_Score=86, Evalue=6e-17,
Organism=Caenorhabditis elegans, GI17533173, Length=278, Percent_Identity=33.4532374100719, Blast_Score=132, Evalue=3e-31,
Organism=Caenorhabditis elegans, GI17556280, Length=295, Percent_Identity=27.1186440677966, Blast_Score=82, Evalue=3e-16,
Organism=Saccharomyces cerevisiae, GI6323383, Length=303, Percent_Identity=26.7326732673267, Blast_Score=102, Evalue=1e-22,
Organism=Drosophila melanogaster, GI21358471, Length=273, Percent_Identity=31.8681318681319, Blast_Score=127, Evalue=1e-29,
Organism=Drosophila melanogaster, GI17933642, Length=289, Percent_Identity=25.6055363321799, Blast_Score=73, Evalue=2e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003010 [H]

Pfam domain/function: PF00795 CN_hydrolase [H]

EC number: =3.5.1.77 [H]

Molecular weight: Translated: 33387; Mature: 33256

Theoretical pI: Translated: 5.90; Mature: 5.90

Prosite motif: PS50263 CN_HYDROLASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.6 %Met     (Translated Protein)
4.3 %Cys+Met (Translated Protein)
1.7 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
4.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPSKHITVAAVQMASGNWNLEDNMATAERLIRAAAAQGANLVLCPELFAMPYFCLDQNVR
CCCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCHHHCCCCC
HLELAQPFEGNAQIARFAALAGELGIVLPIGFFERAGNAAYNSIAVADADGRVLGVYRKT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCEEEEEEEC
HIPDGPGYTEKFYFTPGDTGFKVWDTRFGRIGIGICWDQWYPETARSLALMGAEILCFPT
CCCCCCCCCCEEEECCCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
IIGSEPFSSAFDSSGHWQRTMQGHAAANMVPVVAANRIGREVGFGNGNPEQQGLTGVFYG
HHCCCCCHHHHCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
SSFIADHTGEKRAEANRTDEAVLVHTFDLDAIRADRQSWGFFRDRRPEMYRTLLTSDGVS
CCEEECCCCCHHHHCCCCCCEEEEEEECCHHHHHCHHHCCCHHCCCHHHHHHHHHCCCHH
SCYR
HHCC
>Mature Secondary Structure 
PSKHITVAAVQMASGNWNLEDNMATAERLIRAAAAQGANLVLCPELFAMPYFCLDQNVR
CCCEEEEEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCEEECCHHHHCCCHHHCCCCC
HLELAQPFEGNAQIARFAALAGELGIVLPIGFFERAGNAAYNSIAVADADGRVLGVYRKT
EEEECCCCCCCHHHHHHHHHHCCCCEEEEEHHHHHCCCCCCCEEEEECCCCCEEEEEEEC
HIPDGPGYTEKFYFTPGDTGFKVWDTRFGRIGIGICWDQWYPETARSLALMGAEILCFPT
CCCCCCCCCCEEEECCCCCCCEEECCCCCEEEEEEEECCCCCHHHHHHHHHCCCEEEEEE
IIGSEPFSSAFDSSGHWQRTMQGHAAANMVPVVAANRIGREVGFGNGNPEQQGLTGVFYG
HHCCCCCHHHHCCCCCHHHHHHCCHHHCCHHHHHHHHHHHHCCCCCCCCCCCCCEEEEEC
SSFIADHTGEKRAEANRTDEAVLVHTFDLDAIRADRQSWGFFRDRRPEMYRTLLTSDGVS
CCEEECCCCCHHHHCCCCCCEEEEEEECCHHHHHCHHHCCCHHCCCHHHHHHHHHCCCHH
SCYR
HHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9648217; 10903946 [H]