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The map label for this gene is emrB [H]

Identifier: 78066156

GI number: 78066156

Start: 1680887

End: 1682449

Strand: Direct

Name: emrB [H]

Synonym: Bcep18194_A4686

Alternate gene names: 78066156

Gene position: 1680887-1682449 (Clockwise)

Preceding gene: 78066149

Following gene: 78066157

Centisome position: 45.5

GC content: 64.81

Gene sequence:

>1563_bases
ATGAAGCCGTCCGAAACCCCGGGTACGCCGGCGCCCCTGACCGGCGCCAGCTTCGCGCTGGGCACGCTCGCCGTGTCGCT
CGCCACGTTCATGAACGTTCTCGATTCGTCGATCGCCAACGTCGCGATTCCAACCATTTCCGGCAATCTGGGTGTGTCCA
CGGACGAGGGCACGTGGGTCATCACGCTCTTCTCGGCGGCCAACGCGGTGTCGATCCCGCTGACCGGCTGGCTGACACAG
CGGGTCGGGCAGGTGAAGCTGTTCGTCTGGGCCATCCTGTTGTTCGTGCTGTCGTCGGCCGCGTGCGGACTTGCGCCCAA
CCTCACCACGCTCCTCGCGGCACGGATCGTCCAGGGTGCGGTGGCCGGGCCGCTCGTGCCACTGTCGCAGGCGTTGCTGC
TGGCTTCGTTCCCGAAGGAGAAGAGTTCGAGCGCGCTGGCGCTGTGGGGGATGACCGCGACCGTCGGGCCGATTGCCGGC
CCAGCCCTCGGCGGCTGGATCACGGACAACTACAGTTGGTCGTGGATCTTCTACATCAATGTGCCGGTCGGGTTGTTTGC
CGCCGGCGTGATATGGGCGCTCTATCGCAACCGCGAGACGCCAACCAGCAAGCTGCCGATCGACAAGGTCGGGTTGATTG
CGCTGGTCGTGTGGGTGGCCGCGTTGCAGGTCATGCTCGACAAGGGCAAGGATCTCGACTGGTTCAATTCGTCGACGATC
TGGATCCTGACCATCGTGGCGGTGGTCGGCTTCGTGTTCTTCCTGATCTGGGAGTTGACCGAGGCGCGCCCGATCGTCGA
TCTCAGGCTGTTTGCACAACGAAATTTCCTCGGCGGGACGGTGGCCATTTCGATTGCGTTTGCGGTATTTTTCGCGAATC
TCGTGATCCTGCCGCAATGGATCCAGGGTTCACTCGGCTACCCGGTCATCAATGCAGGCCTCGTGACTGCGCCGCTCGGC
ATCTTCGCGGTGATCCTCGCGCCGGTGGTCGGCAAGGTGATGCCGAAATCGGATATGCGCGTGCTCGTCACGCTCGCCTT
TCTCGGGTTTGCCGGTGTGTTTTTCATGCGCTCGCGCTATACGACGGGCGTCGATACATGGACGCTCATCGTGCCCACCT
TGCTGCAAGGGATCCCGACGGCGTTCTTCATGACGCCGCTTACGTCGATCATCCTGTCGGGGCTGCCGCCCGAGCGCATC
CCGGCGGCCGCGGGACTGTCCAATTTCGCGCGGGTGTTCATGGGGGCGGTCGGCACGTCGCTCGCGAGCACCGCGTGGAA
TGACCGCACGATCCTGCATCACGCGCGACTGGCCGAGCAGGGCAGCGTGAACAACCCGATCTTCGCGGGCGCGCTCTCCG
GCGTGCAGAGTGCGCTGGATGCCGGTGCGCCGAAGGCGCTGACGTTCCTCGAGGCGACGCTGAATGCGCAGGCGACGATG
CTGGGGCTGAACGATGTGTTCTGGCTGTCGTCGGTGATCTTCGTGCTGATCATCCCGCTGGTCTGGGTGACAAGGCCGGG
CAAGGGCGTGGCAGCCGGGGCCGCGGCCGCCGGCGGGCACTGA

Upstream 100 bases:

>100_bases
GTCGCGCCGATCGCTTTTTTTCCGTCATTTATCTGACTTGTCAGATATTTTTATTCGGCGCCGAAGCTGTTTGCGTGGCG
CTCGATCAGAAGGACATCCG

Downstream 100 bases:

>100_bases
GCCGGTGCAGGCGGTGCATGCAGGATTCCGCGTGCCTTGAAAAATCGTGATGTCGCAATTATTGTGTGGGTCAATAAATT
GCGACTCAGCGAGCCCGATG

Product: EmrB/QacA family drug resistance transporter

Products: Proton [Cytoplasm]; multidrug [Periplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 520; Mature: 520

Protein sequence:

>520_residues
MKPSETPGTPAPLTGASFALGTLAVSLATFMNVLDSSIANVAIPTISGNLGVSTDEGTWVITLFSAANAVSIPLTGWLTQ
RVGQVKLFVWAILLFVLSSAACGLAPNLTTLLAARIVQGAVAGPLVPLSQALLLASFPKEKSSSALALWGMTATVGPIAG
PALGGWITDNYSWSWIFYINVPVGLFAAGVIWALYRNRETPTSKLPIDKVGLIALVVWVAALQVMLDKGKDLDWFNSSTI
WILTIVAVVGFVFFLIWELTEARPIVDLRLFAQRNFLGGTVAISIAFAVFFANLVILPQWIQGSLGYPVINAGLVTAPLG
IFAVILAPVVGKVMPKSDMRVLVTLAFLGFAGVFFMRSRYTTGVDTWTLIVPTLLQGIPTAFFMTPLTSIILSGLPPERI
PAAAGLSNFARVFMGAVGTSLASTAWNDRTILHHARLAEQGSVNNPIFAGALSGVQSALDAGAPKALTFLEATLNAQATM
LGLNDVFWLSSVIFVLIIPLVWVTRPGKGVAAGAAAAGGH

Sequences:

>Translated_520_residues
MKPSETPGTPAPLTGASFALGTLAVSLATFMNVLDSSIANVAIPTISGNLGVSTDEGTWVITLFSAANAVSIPLTGWLTQ
RVGQVKLFVWAILLFVLSSAACGLAPNLTTLLAARIVQGAVAGPLVPLSQALLLASFPKEKSSSALALWGMTATVGPIAG
PALGGWITDNYSWSWIFYINVPVGLFAAGVIWALYRNRETPTSKLPIDKVGLIALVVWVAALQVMLDKGKDLDWFNSSTI
WILTIVAVVGFVFFLIWELTEARPIVDLRLFAQRNFLGGTVAISIAFAVFFANLVILPQWIQGSLGYPVINAGLVTAPLG
IFAVILAPVVGKVMPKSDMRVLVTLAFLGFAGVFFMRSRYTTGVDTWTLIVPTLLQGIPTAFFMTPLTSIILSGLPPERI
PAAAGLSNFARVFMGAVGTSLASTAWNDRTILHHARLAEQGSVNNPIFAGALSGVQSALDAGAPKALTFLEATLNAQATM
LGLNDVFWLSSVIFVLIIPLVWVTRPGKGVAAGAAAAGGH
>Mature_520_residues
MKPSETPGTPAPLTGASFALGTLAVSLATFMNVLDSSIANVAIPTISGNLGVSTDEGTWVITLFSAANAVSIPLTGWLTQ
RVGQVKLFVWAILLFVLSSAACGLAPNLTTLLAARIVQGAVAGPLVPLSQALLLASFPKEKSSSALALWGMTATVGPIAG
PALGGWITDNYSWSWIFYINVPVGLFAAGVIWALYRNRETPTSKLPIDKVGLIALVVWVAALQVMLDKGKDLDWFNSSTI
WILTIVAVVGFVFFLIWELTEARPIVDLRLFAQRNFLGGTVAISIAFAVFFANLVILPQWIQGSLGYPVINAGLVTAPLG
IFAVILAPVVGKVMPKSDMRVLVTLAFLGFAGVFFMRSRYTTGVDTWTLIVPTLLQGIPTAFFMTPLTSIILSGLPPERI
PAAAGLSNFARVFMGAVGTSLASTAWNDRTILHHARLAEQGSVNNPIFAGALSGVQSALDAGAPKALTFLEATLNAQATM
LGLNDVFWLSSVIFVLIIPLVWVTRPGKGVAAGAAAAGGH

Specific function: Translocase that confers resistance to substances of high hydrophobicity [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. EmrB family [H]

Homologues:

Organism=Escherichia coli, GI1789042, Length=496, Percent_Identity=49.5967741935484, Blast_Score=467, Evalue=1e-133,
Organism=Escherichia coli, GI1788710, Length=509, Percent_Identity=46.5618860510805, Blast_Score=442, Evalue=1e-125,
Organism=Escherichia coli, GI1790195, Length=384, Percent_Identity=28.3854166666667, Blast_Score=133, Evalue=3e-32,
Organism=Escherichia coli, GI1788392, Length=385, Percent_Identity=30.9090909090909, Blast_Score=120, Evalue=2e-28,
Organism=Escherichia coli, GI87081983, Length=413, Percent_Identity=25.6658595641646, Blast_Score=115, Evalue=8e-27,
Organism=Saccharomyces cerevisiae, GI6322958, Length=200, Percent_Identity=25.5, Blast_Score=65, Evalue=4e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004638
- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 54727; Mature: 54727

Theoretical pI: Translated: 9.86; Mature: 9.86

Prosite motif: PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.1 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
1.9 %Met     (Mature Protein)
2.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MKPSETPGTPAPLTGASFALGTLAVSLATFMNVLDSSIANVAIPTISGNLGVSTDEGTWV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEE
ITLFSAANAVSIPLTGWLTQRVGQVKLFVWAILLFVLSSAACGLAPNLTTLLAARIVQGA
EEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VAGPLVPLSQALLLASFPKEKSSSALALWGMTATVGPIAGPALGGWITDNYSWSWIFYIN
HCCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCCCCCCCCCCEEECCCCEEEEEEEE
VPVGLFAAGVIWALYRNRETPTSKLPIDKVGLIALVVWVAALQVMLDKGKDLDWFNSSTI
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
WILTIVAVVGFVFFLIWELTEARPIVDLRLFAQRNFLGGTVAISIAFAVFFANLVILPQW
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IQGSLGYPVINAGLVTAPLGIFAVILAPVVGKVMPKSDMRVLVTLAFLGFAGVFFMRSRY
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
TTGVDTWTLIVPTLLQGIPTAFFMTPLTSIILSGLPPERIPAAAGLSNFARVFMGAVGTS
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCHHHCHHHHHHHHHHHHHHH
LASTAWNDRTILHHARLAEQGSVNNPIFAGALSGVQSALDAGAPKALTFLEATLNAQATM
HHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEE
LGLNDVFWLSSVIFVLIIPLVWVTRPGKGVAAGAAAAGGH
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC
>Mature Secondary Structure
MKPSETPGTPAPLTGASFALGTLAVSLATFMNVLDSSIANVAIPTISGNLGVSTDEGTWV
CCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHHEEEEEECCCCCCCCCCCCEE
ITLFSAANAVSIPLTGWLTQRVGQVKLFVWAILLFVLSSAACGLAPNLTTLLAARIVQGA
EEEECCCCEEEECHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHHHHHHH
VAGPLVPLSQALLLASFPKEKSSSALALWGMTATVGPIAGPALGGWITDNYSWSWIFYIN
HCCCCHHHHHHHHHHHCCCCCCCCEEEEEECHHHCCCCCCCCCCCEEECCCCEEEEEEEE
VPVGLFAAGVIWALYRNRETPTSKLPIDKVGLIALVVWVAALQVMLDKGKDLDWFNSSTI
CCHHHHHHHHHHHHHHCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCHH
WILTIVAVVGFVFFLIWELTEARPIVDLRLFAQRNFLGGTVAISIAFAVFFANLVILPQW
HHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHCCCCCHHHHHHHHHHHHHHHHHHHHHH
IQGSLGYPVINAGLVTAPLGIFAVILAPVVGKVMPKSDMRVLVTLAFLGFAGVFFMRSRY
HCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHCC
TTGVDTWTLIVPTLLQGIPTAFFMTPLTSIILSGLPPERIPAAAGLSNFARVFMGAVGTS
CCCCCHHHHHHHHHHHHCCHHHHHHHHHHHHHCCCCHHHCCHHHCHHHHHHHHHHHHHHH
LASTAWNDRTILHHARLAEQGSVNNPIFAGALSGVQSALDAGAPKALTFLEATLNAQATM
HHHCCCCCCHHHHHHHHHHCCCCCCCHHHHHHHHHHHHHHCCCCHHHHHHHHHHCCCEEE
LGLNDVFWLSSVIFVLIIPLVWVTRPGKGVAAGAAAAGGH
ECCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; multidrug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + multidrug [Cytoplasm] = Proton [Cytoplasm] + multidrug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]