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The map label for this gene is gloB [H]

Identifier: 78065900

GI number: 78065900

Start: 1385439

End: 1386245

Strand: Direct

Name: gloB [H]

Synonym: Bcep18194_A4429

Alternate gene names: 78065900

Gene position: 1385439-1386245 (Clockwise)

Preceding gene: 161702963

Following gene: 78065901

Centisome position: 37.5

GC content: 67.78

Gene sequence:

>807_bases
ATGAACGAGCTGGAATACGTGCCGGTTCCGGCATTCGATGACAACTATATCTGGCTCGTCTCGGACGGCCGCGATGCGAT
CGCCGTCGATCCGGGTGAAGCCGCGCCGGTGCGCCGGGTTCTAGCCGAACGAGGCTGGCGGTTGACCGCTATTTTACTCA
CGCACCATCACGCCGACCACGTCGGCGGTGTCGCGGCACTGCGCGATAGCCAACCGGACGATGCTCCGCTCACTGTTTAC
GGCCCCGCGGCCGAAGCGATCGGCGTGGTCACGCGGCCGCTTTCGGGCGGCGATCGCGTGACGCTCGACGCGCCCGCCGC
CACGTTCGACGTGCTCGACGTGCCGGGCCATACGCGCGGCCATATCGCCTATTTCCAGGCGGCCGGGCAGGGCAACGCGG
CGCCGCACGTGTTCTGCGGCGACACGCTGTTCTCGTGCGGTTGCGGCCGCCTGTTCGAAGGCACGCCCGCGCAGATGCTC
GCGTCGCTCGACGCGCTCGCGGCGTTGCCGGGCGACACGCACGTGCATTGCGCACACGAATATACGCTGTCCAATATCCG
CTTCGCGCTCGCGTGCGAGCCCGGCAATACGGCGCTCGCCACGTGGCGCGACGATGCGCAAGCGCTGCGCGCGCGCGGCG
TGCCGACGCTGCCCACTACGATCGCGCATGAGCGTGCCGTTAACCCGTTCATGCGGGCGGACAGCGATGCGATCCGCGCG
ACGCTCGAGGCCGAGTTGCACGAAACAGTGCCGGATCGTCTGACGGCGTTCACGCTGATGCGCGAGTGGAAAAACCGGTT
CCGATGA

Upstream 100 bases:

>100_bases
GCGCCTGCTCCCAGCCGAGCACGTAGCGGCCGGGCGGTGAGTCGGTCCAGGCGGGCCAGTCTATAATTTGACGGTCAGAC
ATAACGATGATTGCGCGCCC

Downstream 100 bases:

>100_bases
TGATTTCCGGAGGACTCCAAAGCTCAAGCGGAGTCTGTAAAAATTGCTCCAAATGTAGGATTTCGCTGAGTTTTCGGTGT
TTTTATTGACGTGAAGCACG

Product: hydroxyacylglutathione hydrolase

Products: NA

Alternate protein names: Glyoxalase II; Glx II [H]

Number of amino acids: Translated: 268; Mature: 268

Protein sequence:

>268_residues
MNELEYVPVPAFDDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVAALRDSQPDDAPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAATFDVLDVPGHTRGHIAYFQAAGQGNAAPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTHVHCAHEYTLSNIRFALACEPGNTALATWRDDAQALRARGVPTLPTTIAHERAVNPFMRADSDAIRA
TLEAELHETVPDRLTAFTLMREWKNRFR

Sequences:

>Translated_268_residues
MNELEYVPVPAFDDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVAALRDSQPDDAPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAATFDVLDVPGHTRGHIAYFQAAGQGNAAPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTHVHCAHEYTLSNIRFALACEPGNTALATWRDDAQALRARGVPTLPTTIAHERAVNPFMRADSDAIRA
TLEAELHETVPDRLTAFTLMREWKNRFR
>Mature_268_residues
MNELEYVPVPAFDDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADHVGGVAALRDSQPDDAPLTVY
GPAAEAIGVVTRPLSGGDRVTLDAPAATFDVLDVPGHTRGHIAYFQAAGQGNAAPHVFCGDTLFSCGCGRLFEGTPAQML
ASLDALAALPGDTHVHCAHEYTLSNIRFALACEPGNTALATWRDDAQALRARGVPTLPTTIAHERAVNPFMRADSDAIRA
TLEAELHETVPDRLTAFTLMREWKNRFR

Specific function: Thiolesterase that catalyzes the hydrolysis of S-D- lactoyl-glutathione to form glutathione and D-lactic acid [H]

COG id: COG0491

COG function: function code R; Zn-dependent hydrolases, including glyoxylases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the metallo-beta-lactamase superfamily. Glyoxalase II family [H]

Homologues:

Organism=Homo sapiens, GI94538320, Length=263, Percent_Identity=38.0228136882129, Blast_Score=165, Evalue=3e-41,
Organism=Homo sapiens, GI94538322, Length=263, Percent_Identity=38.0228136882129, Blast_Score=164, Evalue=7e-41,
Organism=Homo sapiens, GI14150041, Length=233, Percent_Identity=38.1974248927039, Blast_Score=143, Evalue=1e-34,
Organism=Homo sapiens, GI116642887, Length=244, Percent_Identity=36.0655737704918, Blast_Score=140, Evalue=2e-33,
Organism=Homo sapiens, GI21703352, Length=244, Percent_Identity=36.0655737704918, Blast_Score=139, Evalue=2e-33,
Organism=Homo sapiens, GI46361987, Length=190, Percent_Identity=40, Blast_Score=119, Evalue=2e-27,
Organism=Escherichia coli, GI1786406, Length=262, Percent_Identity=45.0381679389313, Blast_Score=198, Evalue=3e-52,
Organism=Escherichia coli, GI1787158, Length=193, Percent_Identity=32.6424870466321, Blast_Score=63, Evalue=2e-11,
Organism=Caenorhabditis elegans, GI17536925, Length=260, Percent_Identity=35, Blast_Score=140, Evalue=6e-34,
Organism=Saccharomyces cerevisiae, GI6324614, Length=270, Percent_Identity=30, Blast_Score=87, Evalue=2e-18,
Organism=Saccharomyces cerevisiae, GI6320478, Length=266, Percent_Identity=28.5714285714286, Blast_Score=84, Evalue=2e-17,
Organism=Drosophila melanogaster, GI21356335, Length=239, Percent_Identity=40.1673640167364, Blast_Score=164, Evalue=4e-41,
Organism=Drosophila melanogaster, GI24667711, Length=239, Percent_Identity=40.1673640167364, Blast_Score=164, Evalue=5e-41,
Organism=Drosophila melanogaster, GI24667703, Length=239, Percent_Identity=40.1673640167364, Blast_Score=164, Evalue=7e-41,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001279
- InterPro:   IPR017782 [H]

Pfam domain/function: PF00753 Lactamase_B [H]

EC number: =3.1.2.6 [H]

Molecular weight: Translated: 28917; Mature: 28917

Theoretical pI: Translated: 5.48; Mature: 5.48

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.9 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
3.4 %Cys+Met (Translated Protein)
1.9 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
3.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVAALRDSQPDDAPLTVYGPAAEAIGVVTRPLSGGDRVTLDAPAATFDVLDVPGHTRG
HCCEEEECCCCCCCCCEEEECCCHHHHHHEECCCCCCCEEEEECCCCEEEEEECCCCCCC
HIAYFQAAGQGNAAPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTHVHCAHE
EEEEEEECCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPGNTALATWRDDAQALRARGVPTLPTTIAHERAVNPFMRADSDAIRA
EEECCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHH
TLEAELHETVPDRLTAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHHCC
>Mature Secondary Structure
MNELEYVPVPAFDDNYIWLVSDGRDAIAVDPGEAAPVRRVLAERGWRLTAILLTHHHADH
CCCCCEEECCCCCCCEEEEEECCCCEEEECCCCCHHHHHHHHHCCCEEEEEEEEECCCHH
VGGVAALRDSQPDDAPLTVYGPAAEAIGVVTRPLSGGDRVTLDAPAATFDVLDVPGHTRG
HCCEEEECCCCCCCCCEEEECCCHHHHHHEECCCCCCCEEEEECCCCEEEEEECCCCCCC
HIAYFQAAGQGNAAPHVFCGDTLFSCGCGRLFEGTPAQMLASLDALAALPGDTHVHCAHE
EEEEEEECCCCCCCCEEEECCHHHHCCCCCCCCCCHHHHHHHHHHHHCCCCCCEEEEEEE
YTLSNIRFALACEPGNTALATWRDDAQALRARGVPTLPTTIAHERAVNPFMRADSDAIRA
EEECCEEEEEEECCCCCEEEEECHHHHHHHHCCCCCCCHHHHHHHHCCHHHHCCCHHHHH
TLEAELHETVPDRLTAFTLMREWKNRFR
HHHHHHHHHHHHHHHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA