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The map label for this gene is psdht [H]

Identifier: 78065871

GI number: 78065871

Start: 1361099

End: 1362019

Strand: Direct

Name: psdht [H]

Synonym: Bcep18194_A4400

Alternate gene names: 78065871

Gene position: 1361099-1362019 (Clockwise)

Preceding gene: 78065864

Following gene: 78065874

Centisome position: 36.84

GC content: 71.23

Gene sequence:

>921_bases
ATGCCGCTTCATATCCCGACCCCTTATATCCGCTCGCAAATTGCGTCCCGCCGGCTCGGCAGGACCATCCGGCTGAAGCT
CGATGCGCTGCAGCCGTCGGGCTCGTTCAAGCTGCGCGGCATCGGCGCCGTTTGTGAAGCGCGGCATGCGGCCGGCGCGC
GGCGCTTCGTGTCGTCGTCGGGCGGCAATGCGGGCATCGCGGTCGCGTATTGCGGCCGCGAACTCGGCGTGCCGGTGCTC
GTCGTCGTGCCGGAGAGCGCGTCGGCCCGCGCCCGCGAGCTGATCCGCGTCGAGGGCGCGGAGGTGGTCGTGCACGGCGC
GAGCTGGGCCGAGGCGAACGCGTACGCGCAGTCGGCACTCGGCGAGCACGATGCGTTCGTGCATCCGTTCGACGACCGTG
TGCTGTGGCAGGGCCACGCGACGATGATCGACGAGATGGCGGCGGCCGGCCCGAAGCCCGGCGCGGTCGTGCTCGCGGTC
GGCGGCGGCGGGCTGCTGTGCGGCGTGCTCGAAGGGCTGGCGCGCAACGGCTGGCACGACGTGCCGGTAGTCGCCGCCGA
AACGGAGGGCGCCGACTGCTATGCGCGCTCGCTGGCGCAGGGGCGTGTGGTCGAGTTGTCGGCGATCACGAGCATCGCGA
CGTCGCTCGGCGCGAAGCGGCCATGCGATGCGGCGGTCGAATGGGCCGCGCGACACGAGATCCATCCGGTCGTCGTGTCC
GATGCCGACGCGGTGGCCGCGTCGCTGCGCTTCCTCGATGAACACCGGATCGTTGTCGAGCCCGCATGCGGTGCTGCGCT
GGCCGCACTCGAGCGACCGGTGCCCGTGCTTGCATCGGCGACGGATATCGCCGTGATCGTCTGCGGCGGCGTGACGGCAA
CCGTCGAGCAGATGCAGACGTTGCGCGCGACGTTGCGGTAG

Upstream 100 bases:

>100_bases
AAAGCATGGGAAGCGTGCCGTACGATAACCGTCGTTGCCGCGCGCAGTGGCGCGTCGCGTGCGTTCCTTCAGTTCCCTCC
GTTCCCATTGGTCCCGTCCC

Downstream 100 bases:

>100_bases
GCGAGAGGCCGGCGCAAGCAGGTTTGTCGGAGTCGGTTGGATGGCGGTCGATGATGGATATCCCTGCGATCGTCTGCGCT
GGCGTGACGGCGATCATCGA

Product: L-serine ammonia-lyase

Products: oxobutanoate; NH3; H2O [C]

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 305

Protein sequence:

>306_residues
MPLHIPTPYIRSQIASRRLGRTIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIAVAYCGRELGVPVL
VVVPESASARARELIRVEGAEVVVHGASWAEANAYAQSALGEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPGAVVLAV
GGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSLAQGRVVELSAITSIATSLGAKRPCDAAVEWAARHEIHPVVVS
DADAVAASLRFLDEHRIVVEPACGAALAALERPVPVLASATDIAVIVCGGVTATVEQMQTLRATLR

Sequences:

>Translated_306_residues
MPLHIPTPYIRSQIASRRLGRTIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIAVAYCGRELGVPVL
VVVPESASARARELIRVEGAEVVVHGASWAEANAYAQSALGEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPGAVVLAV
GGGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSLAQGRVVELSAITSIATSLGAKRPCDAAVEWAARHEIHPVVVS
DADAVAASLRFLDEHRIVVEPACGAALAALERPVPVLASATDIAVIVCGGVTATVEQMQTLRATLR
>Mature_305_residues
PLHIPTPYIRSQIASRRLGRTIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSSGGNAGIAVAYCGRELGVPVLV
VVPESASARARELIRVEGAEVVVHGASWAEANAYAQSALGEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPGAVVLAVG
GGGLLCGVLEGLARNGWHDVPVVAAETEGADCYARSLAQGRVVELSAITSIATSLGAKRPCDAAVEWAARHEIHPVVVSD
ADAVAASLRFLDEHRIVVEPACGAALAALERPVPVLASATDIAVIVCGGVTATVEQMQTLRATLR

Specific function: Catalyzes The Formation Of Alpha-Ketobutyrate From Threonine In A Two-Step Reaction. The First Step Is A Dehydration Of Threonine, Followed By Rehydration And Liberation Of Ammonia. Deaminates L-Threonine, But Also L-Serine To A Lesser Extent. [C]

COG id: COG1171

COG function: function code E; Threonine dehydratase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Homo sapiens, GI33469958, Length=321, Percent_Identity=43.3021806853583, Blast_Score=196, Evalue=3e-50,
Organism=Homo sapiens, GI19923959, Length=317, Percent_Identity=45.4258675078864, Blast_Score=192, Evalue=2e-49,
Organism=Homo sapiens, GI11345492, Length=296, Percent_Identity=27.7027027027027, Blast_Score=84, Evalue=2e-16,
Organism=Escherichia coli, GI1790207, Length=275, Percent_Identity=30.5454545454545, Blast_Score=86, Evalue=3e-18,
Organism=Caenorhabditis elegans, GI71991565, Length=296, Percent_Identity=28.3783783783784, Blast_Score=80, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6319788, Length=332, Percent_Identity=34.9397590361446, Blast_Score=194, Evalue=2e-50,
Organism=Saccharomyces cerevisiae, GI6322024, Length=205, Percent_Identity=37.5609756097561, Blast_Score=136, Evalue=4e-33,
Organism=Saccharomyces cerevisiae, GI6322023, Length=121, Percent_Identity=36.3636363636364, Blast_Score=77, Evalue=2e-15,
Organism=Saccharomyces cerevisiae, GI6320930, Length=298, Percent_Identity=24.496644295302, Blast_Score=65, Evalue=9e-12,

Paralogues:

None

Copy number: 1344 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 800 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001926 [H]

Pfam domain/function: PF00291 PALP [H]

EC number: 4.3.1.19 [C]

Molecular weight: Translated: 31829; Mature: 31698

Theoretical pI: Translated: 6.84; Mature: 6.84

Prosite motif: PS00165 DEHYDRATASE_SER_THR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
3.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
1.0 %Met     (Mature Protein)
3.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MPLHIPTPYIRSQIASRRLGRTIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSS
CCCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GGNAGIAVAYCGRELGVPVLVVVPESASARARELIRVEGAEVVVHGASWAEANAYAQSAL
CCCCCEEEEECCHHHCCCEEEEECCCCCHHHHHHHEECCCEEEEECCCCHHHHHHHHHHC
GEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPGAVVLAVGGGGLLCGVLEGLARNGWHD
CCCCCEECCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
VPVVAAETEGADCYARSLAQGRVVELSAITSIATSLGAKRPCDAAVEWAARHEIHPVVVS
CCEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEE
DADAVAASLRFLDEHRIVVEPACGAALAALERPVPVLASATDIAVIVCGGVTATVEQMQT
CHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCEEECCCCEEEEEECCCHHHHHHHHH
LRATLR
HHHHCC
>Mature Secondary Structure 
PLHIPTPYIRSQIASRRLGRTIRLKLDALQPSGSFKLRGIGAVCEARHAAGARRFVSSS
CCCCCCHHHHHHHHHHHCCCEEEEEEEEECCCCCEEEEECHHHHHHHHHHHHHHHHHCC
GGNAGIAVAYCGRELGVPVLVVVPESASARARELIRVEGAEVVVHGASWAEANAYAQSAL
CCCCCEEEEECCHHHCCCEEEEECCCCCHHHHHHHEECCCEEEEECCCCHHHHHHHHHHC
GEHDAFVHPFDDRVLWQGHATMIDEMAAAGPKPGAVVLAVGGGGLLCGVLEGLARNGWHD
CCCCCEECCCCCCEEECCCHHHHHHHHHCCCCCCEEEEEECCCHHHHHHHHHHHHCCCCC
VPVVAAETEGADCYARSLAQGRVVELSAITSIATSLGAKRPCDAAVEWAARHEIHPVVVS
CCEEEECCCCCHHHHHHHHCCCEEEHHHHHHHHHHHCCCCCCHHHHHHHHHCCCCEEEEE
DADAVAASLRFLDEHRIVVEPACGAALAALERPVPVLASATDIAVIVCGGVTATVEQMQT
CHHHHHHHHHHHHCCCEEEECCCCHHHHHHCCCCCEEECCCCEEEEEECCCHHHHHHHHH
LRATLR
HHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: AMP; L-Val; pyridoxal 5'-phosphate [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): 40 {L-Ser}} 5 {L-Ser}} 91 {L-Thr}} 70 {L-Thr}} 11 {L-Thr}} [C]

Substrates: @THRE01.txt*L-threonine!; H2O [C]

Specific reaction: @THRE01.txt*L-threonine! + H2O = oxobutanoate + NH3 + H2O [C]

General reaction: Deamination; elimination of NH alphabeta-position of amino acid [C]

Inhibitor: Ile [C]

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12596884; 8743570 [H]