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The map label for this gene is ynfL [H]

Identifier: 78065870

GI number: 78065870

Start: 1360013

End: 1360933

Strand: Reverse

Name: ynfL [H]

Synonym: Bcep18194_A4399

Alternate gene names: 78065870

Gene position: 1360933-1360013 (Counterclockwise)

Preceding gene: 78065872

Following gene: 78065869

Centisome position: 36.84

GC content: 72.1

Gene sequence:

>921_bases
ATGCGTCAGATCGAATTGCGTCACCTGCGCTACTTCGTGGCCGTCGCGCAAGCCGGCAGCGTGATGGCCGGCGCCCGCGC
GGCCGGCATCGTCCAGCCCGCGCTGTCGCGACAGATCCGCGAACTCGAGGACGCGATCGGCACGCCGCTGCTGGTGCGCC
GCGCAACGGGCGTCACGCTCACGGCGGCCGGCGCCAGCTTCCTGCAGGACGCGACCGGCCTGCTCGCGACGCTGCAGGAC
AGCCGTGAGCGCGCACTGCGCAGCGCGGCCGGCCAGCTGGGCGAGCTGCGGCTCGGTGCGTTGCCGAACTGCCTGCCGTT
GCCCGTCGTCGCAAACGTCCTCAAGTCCTTTCGCGATGCGTGCCCGGACGTGAAGCTGTCGATCGCACCGATGCTGTCGG
CCGAACAGGCGGGCGCGCTGATGCGCGGCCAGCTCGACGGCGGGATCATGGCGTGGCGCCGGGACGAGGCGCCTCACCTG
TCGGGCGTACGGCTGCTGAGCGACCGCTTCGTGCTCGCGATGCCCGCGCCGCCGGGCGGACGCTTCACCGCGCCGCGCAC
GCTGGCCGACGTCGCGAACGAGCCGTTCGTCTGGTTCGATGCGCAGCGCTCCGCCGCGCACCACCGGTTCCTGATGGCGC
AGTGCCAGCAGGCCGGCTTCACGCCGCGCATCGCGCAGGTCGGCAGCGACATTCCGACGTTGATCGGCCTCGTCGCAGCC
GGGATGGGCTGTGCATTCGTGCCGGAAAGCTCGTCGCCGACCTGCCCGCACACGGTCCGGCTCGTCGCGCTCGACGAACT
CGCGAGCCGCTTCGACATCGAGTTCGTGTTCGACGGTGCGGCAATGCCGCCGTCGCCCGTCGTCGCGCGGTTTCTCGAGG
CCGTGCGCGACGCGGCCGGTGAAGCGCGCCAAGCGGGCTGA

Upstream 100 bases:

>100_bases
ACGACGGTTATCGTACGGCACGCTTCCCATGCTTTCCAATACCATCGGATGGCGTTTGCTATGCTTCACAGGTATATCGA
TGAACCGGAACGGAACCCGC

Downstream 100 bases:

>100_bases
GTGACAGCAGATTCCACGACTTCGCCCGGCGCTTGAGCGACACGATTCGCGGTGCCGAATGACGGGCGGAACCCCCTTCC
GTCAAACCGTCGGCTTGGCT

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 306; Mature: 306

Protein sequence:

>306_residues
MRQIELRHLRYFVAVAQAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLVRRATGVTLTAAGASFLQDATGLLATLQD
SRERALRSAAGQLGELRLGALPNCLPLPVVANVLKSFRDACPDVKLSIAPMLSAEQAGALMRGQLDGGIMAWRRDEAPHL
SGVRLLSDRFVLAMPAPPGGRFTAPRTLADVANEPFVWFDAQRSAAHHRFLMAQCQQAGFTPRIAQVGSDIPTLIGLVAA
GMGCAFVPESSSPTCPHTVRLVALDELASRFDIEFVFDGAAMPPSPVVARFLEAVRDAAGEARQAG

Sequences:

>Translated_306_residues
MRQIELRHLRYFVAVAQAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLVRRATGVTLTAAGASFLQDATGLLATLQD
SRERALRSAAGQLGELRLGALPNCLPLPVVANVLKSFRDACPDVKLSIAPMLSAEQAGALMRGQLDGGIMAWRRDEAPHL
SGVRLLSDRFVLAMPAPPGGRFTAPRTLADVANEPFVWFDAQRSAAHHRFLMAQCQQAGFTPRIAQVGSDIPTLIGLVAA
GMGCAFVPESSSPTCPHTVRLVALDELASRFDIEFVFDGAAMPPSPVVARFLEAVRDAAGEARQAG
>Mature_306_residues
MRQIELRHLRYFVAVAQAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLVRRATGVTLTAAGASFLQDATGLLATLQD
SRERALRSAAGQLGELRLGALPNCLPLPVVANVLKSFRDACPDVKLSIAPMLSAEQAGALMRGQLDGGIMAWRRDEAPHL
SGVRLLSDRFVLAMPAPPGGRFTAPRTLADVANEPFVWFDAQRSAAHHRFLMAQCQQAGFTPRIAQVGSDIPTLIGLVAA
GMGCAFVPESSSPTCPHTVRLVALDELASRFDIEFVFDGAAMPPSPVVARFLEAVRDAAGEARQAG

Specific function: Unknown

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1787879, Length=265, Percent_Identity=35.8490566037736, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1788887, Length=249, Percent_Identity=34.5381526104418, Blast_Score=127, Evalue=1e-30,
Organism=Escherichia coli, GI2367136, Length=250, Percent_Identity=28.4, Blast_Score=94, Evalue=9e-21,
Organism=Escherichia coli, GI1787601, Length=249, Percent_Identity=28.5140562248996, Blast_Score=92, Evalue=4e-20,
Organism=Escherichia coli, GI157672245, Length=236, Percent_Identity=28.8135593220339, Blast_Score=83, Evalue=2e-17,
Organism=Escherichia coli, GI1789506, Length=235, Percent_Identity=25.1063829787234, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI1790399, Length=255, Percent_Identity=26.6666666666667, Blast_Score=73, Evalue=2e-14,
Organism=Escherichia coli, GI87081904, Length=148, Percent_Identity=33.1081081081081, Blast_Score=70, Evalue=1e-13,
Organism=Escherichia coli, GI1788297, Length=249, Percent_Identity=25.3012048192771, Blast_Score=66, Evalue=3e-12,
Organism=Escherichia coli, GI1790262, Length=253, Percent_Identity=28.0632411067194, Blast_Score=66, Evalue=3e-12,

Paralogues:

None

Copy number: 10-20 Molecules/Cell [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32452; Mature: 32452

Theoretical pI: Translated: 8.24; Mature: 8.24

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.6 %Cys     (Translated Protein)
2.9 %Met     (Translated Protein)
4.6 %Cys+Met (Translated Protein)
1.6 %Cys     (Mature Protein)
2.9 %Met     (Mature Protein)
4.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MRQIELRHLRYFVAVAQAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLVRRATGVTL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
TAAGASFLQDATGLLATLQDSRERALRSAAGQLGELRLGALPNCLPLPVVANVLKSFRDA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
CPDVKLSIAPMLSAEQAGALMRGQLDGGIMAWRRDEAPHLSGVRLLSDRFVLAMPAPPGG
CCCCEEEHHHHHCHHHHCHHHHCCCCCCEEEEECCCCCCCCCHHEECCCEEEEECCCCCC
RFTAPRTLADVANEPFVWFDAQRSAAHHRFLMAQCQQAGFTPRIAQVGSDIPTLIGLVAA
CCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHH
GMGCAFVPESSSPTCPHTVRLVALDELASRFDIEFVFDGAAMPPSPVVARFLEAVRDAAG
CCCCEECCCCCCCCCCHHEEEEEHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
EARQAG
HHHCCC
>Mature Secondary Structure
MRQIELRHLRYFVAVAQAGSVMAGARAAGIVQPALSRQIRELEDAIGTPLLVRRATGVTL
CCCHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEECCCCEE
TAAGASFLQDATGLLATLQDSRERALRSAAGQLGELRLGALPNCLPLPVVANVLKSFRDA
EECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCHHCCCCCCCCHHHHHHHHHHHHHH
CPDVKLSIAPMLSAEQAGALMRGQLDGGIMAWRRDEAPHLSGVRLLSDRFVLAMPAPPGG
CCCCEEEHHHHHCHHHHCHHHHCCCCCCEEEEECCCCCCCCCHHEECCCEEEEECCCCCC
RFTAPRTLADVANEPFVWFDAQRSAAHHRFLMAQCQQAGFTPRIAQVGSDIPTLIGLVAA
CCCCCHHHHHHCCCCEEEEECCHHHHHHHHHHHHHHHCCCCCHHHHHCCCHHHHHHHHHH
GMGCAFVPESSSPTCPHTVRLVALDELASRFDIEFVFDGAAMPPSPVVARFLEAVRDAAG
CCCCEECCCCCCCCCCHHEEEEEHHHHHHHCCEEEEECCCCCCCCHHHHHHHHHHHHHHC
EARQAG
HHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9097039; 9278503 [H]