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The map label for this gene is lutR [H]

Identifier: 78065748

GI number: 78065748

Start: 1210972

End: 1211733

Strand: Direct

Name: lutR [H]

Synonym: Bcep18194_A4276

Alternate gene names: 78065748

Gene position: 1210972-1211733 (Clockwise)

Preceding gene: 78065746

Following gene: 78065752

Centisome position: 32.78

GC content: 69.95

Gene sequence:

>762_bases
ATGTCCGTGCCTACGCTTCCCGCAGCGCCGCGCCGTCGCGCACGCAGCCTCGCACAAGATGTCGTCGACGCGCTGACCGC
GCAGATCGAAAACGGCACGCTGCGTCCCGGCGACAAGCTGCCGACCGAAACCGAAGTCATGGCGGCGCAGGGCGTAAGCC
GGACGGTCGTGCGCGAAGCGATCTCGCGGATGCAGGCGAGCGGCCTCGTCGAGACGCGCCACGGCATCGGCAGCTTCGTG
CTGGAGCTGTCGCGCCGCCAGACGCTCGGCATCGACCCGGCGACGATCACGACGCTGCGCGACGTGCTCGCGGTGCTGGA
ACTGCGCATCAGCCTCGAAAGCGAATGTGCGAGCCTTGCCGCGCAGCGCGCGAACGATACTGACCTGGCCGCGCTGCGGC
GCGCGCTCGATGCGATCGCATCGGGGGCGGGCGGCGGCCGCGACACCGCGCAGCTCGACTTCCAGTTCCACCTGCAGATC
GCGCAGTCCACCGGCAACCGCTATTTCGTCGACATCATGACGCAGCTCGGCACGTCGATCATCCCGCGCACGCGCGTGAA
TTCGGCCCGCTTTGCCGGCGACGATCTGGAGCGCTACGTCGGCCGCCTGAACCACGAGCACGAGGACATCTACGAGGCGA
TCGCACGCCACGACCCGGAGGCCGCCCGTGCCGCGATGCGCACGCACCTCACCAACAGCCGTGAACGGCTGCGCCGCGCG
CACGAAGCGGCTGAGGCCGAGCGCGACACGCAGGCCGGCTGA

Upstream 100 bases:

>100_bases
CGGATGACTTGTGACGCAGTATAATGAATCATCGGCTTTCGCTCAAACCCCGCGTAAACCCTCGGCTCACATTACGATCA
TTCAAAAAGGAACCGGCCTC

Downstream 100 bases:

>100_bases
CGCACCGGGCGACGTGGCCGGCGTTGTCGCCGGCCCGGTCGAATCGCTTCAACATCAGTCGTCATACGAGATCGATATCC
CTTCATCGATCACCGGGGCC

Product: GntR family transcriptional regulator

Products: NA

Alternate protein names: L-lactate utilization operon repressor [H]

Number of amino acids: Translated: 253; Mature: 252

Protein sequence:

>253_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFV
LELSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLAALRRALDAIASGAGGGRDTAQLDFQFHLQI
AQSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAERDTQAG

Sequences:

>Translated_253_residues
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFV
LELSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLAALRRALDAIASGAGGGRDTAQLDFQFHLQI
AQSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
HEAAEAERDTQAG
>Mature_252_residues
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREAISRMQASGLVETRHGIGSFVL
ELSRRQTLGIDPATITTLRDVLAVLELRISLESECASLAAQRANDTDLAALRRALDAIASGAGGGRDTAQLDFQFHLQIA
QSTGNRYFVDIMTQLGTSIIPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRAH
EAAEAERDTQAG

Specific function: Negatively regulates the transcription of the lutABC operon, which is required for L-lactate utilization. LutR activity is regulated by lactate, since presence of L-lactate, that probably binds to lutR, leads to derepression of the operon. Also appears to

COG id: COG2186

COG function: function code K; Transcriptional regulators

Gene ontology:

Cell location: Cytoplasmic

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH gntR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI87082208, Length=226, Percent_Identity=33.1858407079646, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1790780, Length=214, Percent_Identity=34.1121495327103, Blast_Score=99, Evalue=2e-22,
Organism=Escherichia coli, GI1789353, Length=232, Percent_Identity=26.7241379310345, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI1790032, Length=237, Percent_Identity=28.2700421940928, Blast_Score=77, Evalue=1e-15,
Organism=Escherichia coli, GI48994955, Length=224, Percent_Identity=28.125, Blast_Score=75, Evalue=5e-15,
Organism=Escherichia coli, GI1786303, Length=226, Percent_Identity=32.3008849557522, Blast_Score=71, Evalue=7e-14,
Organism=Escherichia coli, GI1789621, Length=229, Percent_Identity=27.0742358078603, Blast_Score=66, Evalue=2e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011711
- InterPro:   IPR000524
- InterPro:   IPR011991 [H]

Pfam domain/function: PF07729 FCD; PF00392 GntR [H]

EC number: NA

Molecular weight: Translated: 27656; Mature: 27525

Theoretical pI: Translated: 6.90; Mature: 6.90

Prosite motif: PS50949 HTH_GNTR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
2.0 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLVETRHGIGSFVLELSRRQTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDTDLAALRRALDAIASGAGGGRDTAQLDFQFHLQIAQSTGNRYFVDIMTQLGTSI
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHEEEEHHCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAERDTQAG
HHHHHHHHCCCCC
>Mature Secondary Structure 
SVPTLPAAPRRRARSLAQDVVDALTAQIENGTLRPGDKLPTETEVMAAQGVSRTVVREA
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHHHHHCHHHHHHHHH
ISRMQASGLVETRHGIGSFVLELSRRQTLGIDPATITTLRDVLAVLELRISLESECASLA
HHHHHHHCCHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHH
AQRANDTDLAALRRALDAIASGAGGGRDTAQLDFQFHLQIAQSTGNRYFVDIMTQLGTSI
HHCCCCHHHHHHHHHHHHHHCCCCCCCCCEEEEHHHEEEEHHCCCCCHHHHHHHHHCHHH
IPRTRVNSARFAGDDLERYVGRLNHEHEDIYEAIARHDPEAARAAMRTHLTNSRERLRRA
CCCCCCCCHHCCHHHHHHHHHHHCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHH
HEAAEAERDTQAG
HHHHHHHHCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9384377 [H]