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The map label for this gene is otsA [H]

Identifier: 78065742

GI number: 78065742

Start: 1202501

End: 1203880

Strand: Direct

Name: otsA [H]

Synonym: Bcep18194_A4270

Alternate gene names: 78065742

Gene position: 1202501-1203880 (Clockwise)

Preceding gene: 78065741

Following gene: 78065744

Centisome position: 32.55

GC content: 66.23

Gene sequence:

>1380_bases
ATGAGTCGCCTCATCATTGTTTCAAACCGCGTTGCGCCGATTTCGGAAGGCGAACCGGCGGCGGGTGGTCTCGCGATCGG
CGTCTACGATGCGCTGAAGGAGACGGGCGGCATGTGGTTCGGCTGGAGCGGCGAGGTCGTCGCGTCCGGCGCACCGCAAA
TTCGCGTCGAGGAGCACGGGCCGGTCACGTTCGCGACCATCGGCCTGTCGCGGCGCGACTACGACCAGTATTACCGCGGC
TTCTCGAACGCGACGCTGTGGCCCGCGTTTCATTACCGCACCGACCTGATCCAGTACGACCGTCACGAGTTCGAAGGCTA
CAGCCGCGTGAACGTGTGGCTTGCCCAGCAGCTCGTGCCGCTGCTGCAGGACGACGACGTGATCTGGGTGCACGACTATC
ACCTGATCCCGTTCGCGCGCGCACTGCGCGCGGCCGGCGTGAAGAACCGCATCGGCTTCTTCCTGCATATCCCGTTTCCG
GCTGCACAGGTGCTCGTCAACGTGCCGCCGCATCGCGATCTCGTCGAGTCACTGTGCGCGTTCGACCTGATCGGTTTCCA
GACCGAACCCGACCTGCGCGCGTTCTGCGACTACGTCGAATCCGAAGCGGACGGCGTGATCGAGCGCGACGGCCACCTGG
CGACGGTGCGCGCATTCGGCCGGACGCTGCGCGCGGCCGCCTATCCGATCGGCGTGCATCCGGACGAGATCGCGTCGCTC
GCGCAGGCCGGCGAGCACGGCAAGGCGGTGCGCACGCTCGCGATCTCGCTGCGCGGCCGCAAGCTGATCATGAGCGTCGA
CCGGCTCGATTATTCGAAGGGGCTCGTCGAGCGCTTCCGTGCGTTCGAGAAGCTGCTCGAGCACGAGCCGTCGCATCGCA
ATCGCGTGTCGTTCCTGCAGATCGCGCCGTCGACCCGCGCGGAGCTGCGCGCGTACCAGGACATCCGGCTACAGCTCGAA
GGGGAGTCGGGGCGCATCAACGGACGCTTCGCCGAGCTCGACTGGGCGCCGATCCTCTATATCCATCGTCAGTACGACCG
CCAGCTGCTCGCTGCGCTGTACCGGCTCGCGCGGGTCGGCTACGTGACGCCGCTGCGCGACGGGATGAATCTCGTCGCGA
AGGAATACGTGTCCGCGCAGAATCCGGAGGATCCGGGCGTGCTCGTACTGTCGCGCTTCGCGGGCGCCGCACGCGAGTTG
ACGGGGGCGCTGATCGTCAATCCGATCGATATCGACGGAATGGCCGACGCGTTGTCGCAGGCGCTGACGATGCCGCTCGC
CGAGCGGCGCGCGCGTTATACGGACATGATTGCGCAGCTTCGCGAGAACAACGTGTCGGTGTGGCGCGACAACTTCCTGC
GCGATCTTCAGCACGCGTAA

Upstream 100 bases:

>100_bases
AGGTCGGCGCGGGCGAGACGTCCGCCCGCATGCGGCTCGATTCGGTCGACGCGCTGCATGAGCAGATCGCGTGCTGGCTT
GGTGCGGGGCAGTCGGACGC

Downstream 100 bases:

>100_bases
CCGGCGTCATCGAGCAATAAAAAAAGCCGCAGTGCGACGCGCACTGCGGCTTTTTTGTTGGGTGCGGGCCGGCGTGTCAG
GCCGCGCGTTCGCCGGTCGG

Product: Alpha,alpha-trehalose-phosphate synthase (UDP-forming)

Products: NA

Alternate protein names: Osmoregulatory trehalose synthesis protein A; Trehalose-6-phosphate synthase; UDP-glucose-glucosephosphate glucosyltransferase [H]

Number of amino acids: Translated: 459; Mature: 458

Protein sequence:

>459_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRG
FSNATLWPAFHYRTDLIQYDRHEFEGYSRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRDLVESLCAFDLIGFQTEPDLRAFCDYVESEADGVIERDGHLATVRAFGRTLRAAAYPIGVHPDEIASL
AQAGEHGKAVRTLAISLRGRKLIMSVDRLDYSKGLVERFRAFEKLLEHEPSHRNRVSFLQIAPSTRAELRAYQDIRLQLE
GESGRINGRFAELDWAPILYIHRQYDRQLLAALYRLARVGYVTPLRDGMNLVAKEYVSAQNPEDPGVLVLSRFAGAAREL
TGALIVNPIDIDGMADALSQALTMPLAERRARYTDMIAQLRENNVSVWRDNFLRDLQHA

Sequences:

>Translated_459_residues
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRG
FSNATLWPAFHYRTDLIQYDRHEFEGYSRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFP
AAQVLVNVPPHRDLVESLCAFDLIGFQTEPDLRAFCDYVESEADGVIERDGHLATVRAFGRTLRAAAYPIGVHPDEIASL
AQAGEHGKAVRTLAISLRGRKLIMSVDRLDYSKGLVERFRAFEKLLEHEPSHRNRVSFLQIAPSTRAELRAYQDIRLQLE
GESGRINGRFAELDWAPILYIHRQYDRQLLAALYRLARVGYVTPLRDGMNLVAKEYVSAQNPEDPGVLVLSRFAGAAREL
TGALIVNPIDIDGMADALSQALTMPLAERRARYTDMIAQLRENNVSVWRDNFLRDLQHA
>Mature_458_residues
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEEHGPVTFATIGLSRRDYDQYYRGF
SNATLWPAFHYRTDLIQYDRHEFEGYSRVNVWLAQQLVPLLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPA
AQVLVNVPPHRDLVESLCAFDLIGFQTEPDLRAFCDYVESEADGVIERDGHLATVRAFGRTLRAAAYPIGVHPDEIASLA
QAGEHGKAVRTLAISLRGRKLIMSVDRLDYSKGLVERFRAFEKLLEHEPSHRNRVSFLQIAPSTRAELRAYQDIRLQLEG
ESGRINGRFAELDWAPILYIHRQYDRQLLAALYRLARVGYVTPLRDGMNLVAKEYVSAQNPEDPGVLVLSRFAGAARELT
GALIVNPIDIDGMADALSQALTMPLAERRARYTDMIAQLRENNVSVWRDNFLRDLQHA

Specific function: Catalyzes the transfer of glucose from UDP-glucose to glucose-6-phosphate to form alpha,alpha-1,1 trehalose-6-phosphate. Acts with retention of the anomeric configuration of the UDP-sugar donor [H]

COG id: COG0380

COG function: function code G; Trehalose-6-phosphate synthase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the glycosyltransferase 20 family [H]

Homologues:

Organism=Escherichia coli, GI1788206, Length=458, Percent_Identity=53.4934497816594, Blast_Score=483, Evalue=1e-137,
Organism=Caenorhabditis elegans, GI115533558, Length=417, Percent_Identity=29.2565947242206, Blast_Score=155, Evalue=5e-38,
Organism=Caenorhabditis elegans, GI115533560, Length=419, Percent_Identity=29.1169451073986, Blast_Score=155, Evalue=6e-38,
Organism=Caenorhabditis elegans, GI71987012, Length=416, Percent_Identity=29.3269230769231, Blast_Score=149, Evalue=4e-36,
Organism=Saccharomyces cerevisiae, GI6319602, Length=470, Percent_Identity=32.1276595744681, Blast_Score=270, Evalue=3e-73,
Organism=Saccharomyces cerevisiae, GI6323537, Length=434, Percent_Identity=25.8064516129032, Blast_Score=165, Evalue=1e-41,
Organism=Saccharomyces cerevisiae, GI6320279, Length=403, Percent_Identity=26.0545905707196, Blast_Score=159, Evalue=1e-39,
Organism=Saccharomyces cerevisiae, GI6323917, Length=376, Percent_Identity=25.531914893617, Blast_Score=151, Evalue=2e-37,
Organism=Drosophila melanogaster, GI19920676, Length=486, Percent_Identity=34.7736625514403, Blast_Score=274, Evalue=1e-73,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001830
- InterPro:   IPR012766 [H]

Pfam domain/function: PF00982 Glyco_transf_20 [H]

EC number: =2.4.1.15 [H]

Molecular weight: Translated: 51629; Mature: 51498

Theoretical pI: Translated: 6.64; Mature: 6.64

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
1.5 %Met     (Translated Protein)
2.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
1.3 %Met     (Mature Protein)
1.7 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEEHG
CCCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCEEEEECCC
PVTFATIGLSRRDYDQYYRGFSNATLWPAFHYRTDLIQYDRHEFEGYSRVNVWLAQQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRDLVESLCA
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCCHHHHHHHHH
FDLIGFQTEPDLRAFCDYVESEADGVIERDGHLATVRAFGRTLRAAAYPIGVHPDEIASL
HHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHH
AQAGEHGKAVRTLAISLRGRKLIMSVDRLDYSKGLVERFRAFEKLLEHEPSHRNRVSFLQ
HHCCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IAPSTRAELRAYQDIRLQLEGESGRINGRFAELDWAPILYIHRQYDRQLLAALYRLARVG
ECCCCHHHHHHEEEEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCC
YVTPLRDGMNLVAKEYVSAQNPEDPGVLVLSRFAGAARELTGALIVNPIDIDGMADALSQ
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHCCEEEEECCCCCHHHHHHHH
ALTMPLAERRARYTDMIAQLRENNVSVWRDNFLRDLQHA
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SRLIIVSNRVAPISEGEPAAGGLAIGVYDALKETGGMWFGWSGEVVASGAPQIRVEEHG
CCEEEEECCCCCCCCCCCCCCCEEEHHHHHHHHCCCEEECCCCCEEECCCCEEEEECCC
PVTFATIGLSRRDYDQYYRGFSNATLWPAFHYRTDLIQYDRHEFEGYSRVNVWLAQQLVP
CEEEEEECCCCCCHHHHHCCCCCCEECCCHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHH
LLQDDDVIWVHDYHLIPFARALRAAGVKNRIGFFLHIPFPAAQVLVNVPPHRDLVESLCA
HHCCCCEEEEECCCHHHHHHHHHHCCCCCCCCEEEECCCCHHHHEEECCCCHHHHHHHHH
FDLIGFQTEPDLRAFCDYVESEADGVIERDGHLATVRAFGRTLRAAAYPIGVHPDEIASL
HHHHCCCCCCCHHHHHHHHHHCCCCCEECCCCEEHHHHHHHHHHHHCCCCCCCHHHHHHH
AQAGEHGKAVRTLAISLRGRKLIMSVDRLDYSKGLVERFRAFEKLLEHEPSHRNRVSFLQ
HHCCCCCCCEEEEEEEECCCEEEEEHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCEEEEE
IAPSTRAELRAYQDIRLQLEGESGRINGRFAELDWAPILYIHRQYDRQLLAALYRLARVG
ECCCCHHHHHHEEEEEEEEECCCCCCCCEEEECCCCEEEEHHHHHHHHHHHHHHHHHHCC
YVTPLRDGMNLVAKEYVSAQNPEDPGVLVLSRFAGAARELTGALIVNPIDIDGMADALSQ
CCCCCHHHHHHHHHHHHHCCCCCCCCEEEEHHHCCHHHHHCCEEEEECCCCCHHHHHHHH
ALTMPLAERRARYTDMIAQLRENNVSVWRDNFLRDLQHA
HHHCHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA