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The map label for this gene is tolB

Identifier: 78065363

GI number: 78065363

Start: 805671

End: 806966

Strand: Reverse

Name: tolB

Synonym: Bcep18194_A3889

Alternate gene names: 78065363

Gene position: 806966-805671 (Counterclockwise)

Preceding gene: 78065364

Following gene: 78065362

Centisome position: 21.84

GC content: 64.58

Gene sequence:

>1296_bases
ATGAGTTTGATGACAAAGCTAGGTTTCAGGGCACTCGTGGCCTCGTGTCTGATTACGGCGGGCAGCGCCGCTAACGCCCA
GGTCAACGTGCTGATCACCGGTGTCGGGTCGACCCAGTTCCCCATCGCCACCGCGAATTTCGCGAACGAGGCGAACCTGC
CGCAGCAGGTCACGTCGATCGTGCGCGCCGACCTCGCCCGCAGTGGCAAATTCACCAACATCGACGCAGGCAGCACACCC
GTGCCGGAGTCCGCATCGGTCGATCTCGGCGCATGGAAGGCCAAGGGCGCGAATGCGTTCGTCGCCGGCAGCGTAAACCG
CGAGGCGAACGGCCAGTACAAGGTCAACTTCATCCTGTACGACACCGTGAAGCAGCAAAGCCTCGGCGGCCTGTCGCTGA
CGGCCACCGACACCACGCTGCGCACGGCCGGCCACAAGATCGCCGACTACATCTACCAGAAGCTTCTCGGCGTGCGCGGC
GTGTTCGCCACGCGCCTGTCGTACGTGATCAAGACCGGTAACCGCTACCAGCTGCAGATTTCGGATTCGGACGGCCAGAA
CGCGCGTATCGCACTGTCGAGCACCGAGCCGATCATCTCGCCGTCCTGGTCGCCGAGCGGCACGAAGGTCGCGTACGTGT
CGTTCGAGCGCAAGAAGCCGATCGTCTACATCCACGACCTGCCGACCGGCCGTCGCTACATCGTCTCCGACCAGAAGGGC
AACAACAGCGCACCGGCGTGGTCGCCGGACAGCAACACGCTGGCCGTCGCGCTGTCGCTGACGGGCAATACGCAAATCTA
TACGGTCAACTCGACGGGCGGCGGCCTGCGCCGCCTCACGCAGAGCAGCTCGATCGACACCGAGCCGTTCTACTCGCCGG
ATGGCCACTGGATCTACTTCACGAGCGATCGCGGCGGCGCGCCGCAGATCTACCGGATGCCCGCACAGGGCGAAAGCGCC
GGTGCTGCGCAGCGCGTGACTTTCACCGGCAGCTACAACACGAGTCCGCGCATCAGCCCGGACGGCAAGCTGCTTGCCTA
CATCTCCCGCACGGGCGGGGGCTTCAAGCTGTACGTTCAGGATCTGCAGTCTGGCGCGGCGAACGCCATCACGAATACGA
ATCGCGACGAATCGCCGAGCTTCGCGGCAAACGGCCAGTACCTTCTGTACGCTACCCAGTCGGGTGGTCGCAATGTTCTG
GCAGCAGTGCCCTCCGACGGCAGCGCGCCGCCGCAAATCCTGTCCGTCCAGGGCGGCTCCGTACGTGAGCCGTCGTGGGG
GCCCTTCATGCAATGA

Upstream 100 bases:

>100_bases
CGCTTACACTCCGGGCGTCGCTGTCGGACGGGAACGAATCGGGTATTTTTACTGTCTCTGCCGTTGCGCAGCGATCCAAG
CCATACGGGAAGCAACAAGC

Downstream 100 bases:

>100_bases
CCACAAGGAGAGTAAACATGATGTCGAATAAAGCTCGTCTGGCCCTGGCCGTGATGATGATCAGCGCGCTCGCAGCGTGC
AAGTCGGGCGTGAAGCTCGA

Product: translocation protein TolB

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 431; Mature: 430

Protein sequence:

>431_residues
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFANEANLPQQVTSIVRADLARSGKFTNIDAGSTP
VPESASVDLGAWKAKGANAFVAGSVNREANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPSWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYIVSDQKG
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNSTGGGLRRLTQSSSIDTEPFYSPDGHWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQSGAANAITNTNRDESPSFAANGQYLLYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Sequences:

>Translated_431_residues
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFANEANLPQQVTSIVRADLARSGKFTNIDAGSTP
VPESASVDLGAWKAKGANAFVAGSVNREANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRG
VFATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPSWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYIVSDQKG
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNSTGGGLRRLTQSSSIDTEPFYSPDGHWIYFTSDRGGAPQIYRMPAQGESA
GAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQSGAANAITNTNRDESPSFAANGQYLLYATQSGGRNVL
AAVPSDGSAPPQILSVQGGSVREPSWGPFMQ
>Mature_430_residues
SLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFANEANLPQQVTSIVRADLARSGKFTNIDAGSTPV
PESASVDLGAWKAKGANAFVAGSVNREANGQYKVNFILYDTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGV
FATRLSYVIKTGNRYQLQISDSDGQNARIALSSTEPIISPSWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYIVSDQKGN
NSAPAWSPDSNTLAVALSLTGNTQIYTVNSTGGGLRRLTQSSSIDTEPFYSPDGHWIYFTSDRGGAPQIYRMPAQGESAG
AAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQDLQSGAANAITNTNRDESPSFAANGQYLLYATQSGGRNVLA
AVPSDGSAPPQILSVQGGSVREPSWGPFMQ

Specific function: Involved in the tonB-independent uptake of proteins [H]

COG id: COG0823

COG function: function code U; Periplasmic component of the Tol biopolymer transport system

Gene ontology:

Cell location: Periplasm (Potential) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the tolB family [H]

Homologues:

Organism=Escherichia coli, GI1786961, Length=439, Percent_Identity=37.129840546697, Blast_Score=254, Evalue=9e-69,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011659
- InterPro:   IPR014167
- InterPro:   IPR007195
- InterPro:   IPR015943 [H]

Pfam domain/function: PF07676 PD40; PF04052 TolB_N [H]

EC number: NA

Molecular weight: Translated: 45722; Mature: 45590

Theoretical pI: Translated: 9.85; Mature: 9.85

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.2 %Cys     (Translated Protein)
0.9 %Met     (Translated Protein)
1.2 %Cys+Met (Translated Protein)
0.2 %Cys     (Mature Protein)
0.7 %Met     (Mature Protein)
0.9 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFANEANLPQQVTSI
CCHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPESASVDLGAWKAKGANAFVAGSVNREANGQYKVNFILY
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPSWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYIVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNSTGGGLRRLTQSSSIDTEPFYSPDGHWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQSGAANAITNTNRDESPSFAANGQYLLYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHHCCCCCCCCCCEECCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC
>Mature Secondary Structure 
SLMTKLGFRALVASCLITAGSAANAQVNVLITGVGSTQFPIATANFANEANLPQQVTSI
CHHHHHHHHHHHHHHHHCCCCCCCEEEEEEEEECCCCCCCEEECCCCCCCCCHHHHHHH
VRADLARSGKFTNIDAGSTPVPESASVDLGAWKAKGANAFVAGSVNREANGQYKVNFILY
HHHHHHHCCCEEECCCCCCCCCCCCCCCCCCEECCCCCEEEEECCCCCCCCEEEEEEEEE
DTVKQQSLGGLSLTATDTTLRTAGHKIADYIYQKLLGVRGVFATRLSYVIKTGNRYQLQI
ECCHHCCCCCEEEEECCCCHHHCCHHHHHHHHHHHHHHHHHHHHHHHHEEECCCEEEEEE
SDSDGQNARIALSSTEPIISPSWSPSGTKVAYVSFERKKPIVYIHDLPTGRRYIVSDQKG
ECCCCCCEEEEEECCCCCCCCCCCCCCCEEEEEEEECCCCEEEEEECCCCCEEEEECCCC
NNSAPAWSPDSNTLAVALSLTGNTQIYTVNSTGGGLRRLTQSSSIDTEPFYSPDGHWIYF
CCCCCCCCCCCCEEEEEEEECCCEEEEEEECCCCHHHHHHCCCCCCCCCCCCCCCCEEEE
TSDRGGAPQIYRMPAQGESAGAAQRVTFTGSYNTSPRISPDGKLLAYISRTGGGFKLYVQ
ECCCCCCCEEEECCCCCCCCCCEEEEEEECCCCCCCCCCCCCCEEEEEECCCCCEEEEEE
DLQSGAANAITNTNRDESPSFAANGQYLLYATQSGGRNVLAAVPSDGSAPPQILSVQGGS
ECCCCCCHHHCCCCCCCCCCEECCCEEEEEEECCCCCEEEEECCCCCCCCCEEEEECCCC
VREPSWGPFMQ
CCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA