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The map label for this gene is gspO [H]

Identifier: 78065137

GI number: 78065137

Start: 546856

End: 547767

Strand: Reverse

Name: gspO [H]

Synonym: Bcep18194_A3661

Alternate gene names: 78065137

Gene position: 547767-546856 (Counterclockwise)

Preceding gene: 78065138

Following gene: 78065136

Centisome position: 14.83

GC content: 69.52

Gene sequence:

>912_bases
ATGACGCCAGCGCCCCTGTCCGCCGTTCCCGATTCGCCCGAGAGCACGCTGCTCGCGCTGGCGATGCTGCCGCCCGCGCT
GCAGTATGCGTTCGCGGTCGTCATCGGCCTTTGCATCGGCAGCTTCCTGAATGTGGTCGTGCACCGGCTGCCCGTGATGA
TGCAGCGCGCGTGGCAGGCCGAGATCGACGAAGCGACCGGCAACGCGAGCGCCCCGCCCGACGACGGCTACCCGCCGCGC
TACGATCTGTGGCGCCCGCGCAGCGCCTGCCCGCATTGCGGCCACGTGCTGCGCGCATGGGAAAACATCCCGCTCGTCAG
CTACCTGATGCTGCGCGGGCGCTGCCGGCGCTGCGGCCACGCGATCGGCATCCGCTATCCGCTCGTCGAACTCGCGTGCG
CGCTGCTCGCCGCCGGCTCGCTCGCGGCATTTGGCCCGACCGTCGCCGCGCTTGCCGCGTTCGCGCTGTGCGCCGCGCTA
CTCGCGATGAGCGCGATCGATATCCGGACCGGCTACCTGCCCGACTCGATGACGCTGCCGCTCCTCTGGGCCGGGCTCGT
GTTGAACCTCGGTGGCACGTTCACATCGCTGCGCTCGGCCGTGATCGGGGCCATGGCCGGCTACCTGTTCCTGTGGTCGA
TCTACTGGCTGTTCAAATGGCTGCGCGGCATCGAGGGCATTGGTTTCGGCGACCTGAAGCTGCTCGCCGCGCTCGGCGCA
TGGATGGGCTGGGCCGCATTGCCGCAAGTGGTGCTGTTCGCCGCGGTGACGGGTGCGATCGTCGGGCTCATTGCGACCTG
GCGCGGCCGCATGCGCTTCGAGGAGCCGATTCCGTTCGGCCCGTTCCTCGCGGCAGGCGGCGTCGCGACGCTGTTTTTCG
GCACCCCTTTCTATTCGGCGCTCGGAGGCTGA

Upstream 100 bases:

>100_bases
CGGCCTCGTGATCGCGATGTACCTGCCCATCCTTCAGCTCGGCAACGTGGTGTAGCATCGCAACCGATTCTGTCGCCTTT
TAGTCCGAACCTTGAGCCTC

Downstream 100 bases:

>100_bases
CATGTTCGCAATCGGACTCACCGGCGGCATCGGCAGCGGCAAGACGACCGTCGCCGACCTGTTCGGCGCCCGCGGCGCGT
CGCTGGTCGATACCGATCTG

Product: type 4 prepilin peptidase 1. Aspartic peptidase. MEROPS family A24A

Products: NA

Alternate protein names: Leader peptidase; Prepilin peptidase; N-methyltransferase [H]

Number of amino acids: Translated: 303; Mature: 302

Protein sequence:

>303_residues
MTPAPLSAVPDSPESTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIDEATGNASAPPDDGYPPR
YDLWRPRSACPHCGHVLRAWENIPLVSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAALAAFALCAAL
LAMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
WMGWAALPQVVLFAAVTGAIVGLIATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYSALGG

Sequences:

>Translated_303_residues
MTPAPLSAVPDSPESTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIDEATGNASAPPDDGYPPR
YDLWRPRSACPHCGHVLRAWENIPLVSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAALAAFALCAAL
LAMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
WMGWAALPQVVLFAAVTGAIVGLIATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYSALGG
>Mature_302_residues
TPAPLSAVPDSPESTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQAEIDEATGNASAPPDDGYPPRY
DLWRPRSACPHCGHVLRAWENIPLVSYLMLRGRCRRCGHAIGIRYPLVELACALLAAGSLAAFGPTVAALAAFALCAALL
AMSAIDIRTGYLPDSMTLPLLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGAW
MGWAALPQVVLFAAVTGAIVGLIATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYSALGG

Specific function: Cleaves type-4 fimbrial leader sequence and methylates the N-terminal (generally Phe) residue [H]

COG id: COG1989

COG function: function code NOU; Type II secretory pathway, prepilin signal peptidase PulO and related peptidases

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase A24 family [H]

Homologues:

Organism=Escherichia coli, GI87082194, Length=268, Percent_Identity=39.1791044776119, Blast_Score=162, Evalue=2e-41,
Organism=Escherichia coli, GI1789732, Length=148, Percent_Identity=44.5945945945946, Blast_Score=87, Evalue=2e-18,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR010627
- InterPro:   IPR014032
- InterPro:   IPR000045 [H]

Pfam domain/function: PF06750 DiS_P_DiS; PF01478 Peptidase_A24 [H]

EC number: =3.4.23.43 [H]

Molecular weight: Translated: 32323; Mature: 32191

Theoretical pI: Translated: 8.27; Mature: 8.27

Prosite motif: PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.3 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.3 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTPAPLSAVPDSPESTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQA
CCCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH
EIDEATGNASAPPDDGYPPRYDLWRPRSACPHCGHVLRAWENIPLVSYLMLRGRCRRCGH
HHHHCCCCCCCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHCCHHHHHHHHHHHHHHHHH
AIGIRYPLVELACALLAAGSLAAFGPTVAALAAFALCAALLAMSAIDIRTGYLPDSMTLP
HHCCHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCHHH
LLWAGLVLNLGGTFTSLRSAVIGAMAGYLFLWSIYWLFKWLRGIEGIGFGDLKLLAALGA
HHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCHHHHHHHHHHH
WMGWAALPQVVLFAAVTGAIVGLIATWRGRMRFEEPIPFGPFLAAGGVATLFFGTPFYSA
HHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCCHHHHCCHHHHHHCCHHHHH
LGG
HCC
>Mature Secondary Structure 
TPAPLSAVPDSPESTLLALAMLPPALQYAFAVVIGLCIGSFLNVVVHRLPVMMQRAWQA
CCCCCCCCCCCCHHHHHHHHHHCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHH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HCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 10419967 [H]