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The map label for this gene is murD

Identifier: 78065119

GI number: 78065119

Start: 527338

End: 528849

Strand: Direct

Name: murD

Synonym: Bcep18194_A3643

Alternate gene names: 78065119

Gene position: 527338-528849 (Clockwise)

Preceding gene: 78065118

Following gene: 78065120

Centisome position: 14.28

GC content: 70.04

Gene sequence:

>1512_bases
ATGTTTGGAGATCGGCAACGGCCGATGGTGCTCGTACTGGGGCTCGGGGAATCGGGTCTCGCGATCGCGCGATGGTGCGC
GAGGCACGGGTGCCGGCTGCGTATTGCCGATACCCGCGAGGCGCCGCCGAACCTTGCCGCGCTGCAGGCCGAAGGCATCG
ATGCGGAATTCGTCGGCGGGGCGTTCACGCCTGCGCTGCTCGACGGCGGTATCGAGCTCGTCGGGCTGAGCCCCGGGCTG
TCGCCGCTCGAACCGGCACTCGCAGCGCTGGTCGCAGCTGCGAACGAGCAGGGCGTCGCGGTGTGGGGCGAGCTGGAATT
CTTCGCGCAGGCGCTGCGTGCACTCGGCACGAGCGGCTACCAGCCGAAGGTGCTCGCGATCACGGGCACCAACGGCAAGA
CTACGACGACGAGCCTCACGGGCCTGCTGTGCCAGCGCTCGGGCAAGAAGGTCGCCGTCGCGGGCAACATCAGCCCCGCG
ATGCTCGACCGGCTCGCCGCCGCGATCGACGAAACCGCGCTGCCCGACGTGTGGGTGCTCGAACTGTCGAGCTTCCAGCT
CGAGACCGCGCGCACGTTCGCGCCCGATGCGGCCGCGATCCTCAACATCACGCAGGACCACCTCGACTGGCACGGCAGCT
TCGACGCGTACGCGCAGGCAAAGGGCCGGATCTTCGGCGCAACGACCACGCGCGTGCTGAACCGCGACGATGCGGCCGTA
ATGAAGTTCGCGCCGGCAGCCGGCGCGGCCGATGCGCCGCGCACGGTCACGTTCGGCCTGAACGAACCGGTGCAGGACGG
CGACTACGGGCTGTCGCGCGACAACGGCATCGCGTGGCTGGTGGAAGCCGTCGATCGCGACGCCCCGGATGAAGCTGCGA
CGACGACCCGTCGGCGCAAGCGCGACGCCCATACGCCCGACATCGCGCAGAAGCGCCTGATGCCGGCCGACGCCCTGCGC
ATCCGCGGGCTGCACAACGCGGCGAACGCGCTGGCCGCATTCGCGCTCGCACGCGCGATCGACCTGCCGGCCGCGCCGCT
GCTGCACGCGCTGCGCGAATACCGCGGCGAGGCGCATCGCGTCGAAGTGATCGCGACGATCGACGACGTGGACTACGTCG
ACGACAGCAAGGGTACGAACGTCGGCGCGACGGTTGCCGCGCTCGACGGGCTGGCACAGAAGATCGTGCTGATCGCGGGC
GGCGACGGCAAGGGGCAGGATTTCGCACCGCTGGTTGCGCCGGTCGCGCGCTGGTGCCGCGCGGTGATGCTGATCGGCCG
TGATGCGCCGGCGATCCGCGACACGCTCGCCGAAACGGGTGTGCCGCTCGCCGACCACGTGACGCTCGAAGGGGCGGTGC
ACGCGGCAGCCGACCTCGCCGAACCGGGCGATGCAGTGCTGCTGTCGCCTGCATGCGCGAGCCTCGACATGTTCAGGAAC
TACGCCCATCGCGCGGATGTGTTCCGCGCGGCGGTCGACGAAATCGCCATCGACAAAGGAGCGACGCCATGA

Upstream 100 bases:

>100_bases
GTGGTCGTGCGTTTCTGGATCATCACGCTGATGCTGTGCCTGTTCGGTCTGACCACCCTCAAGCTGCGGTAAAGGAAAGG
TAACAAGGATGTCTGGCGAG

Downstream 100 bases:

>100_bases
GCTGGTCCGATCGCCTCGTCTCCCGTTTCAACGATCGCCGCGACACCGGCGGCAATGCCGCGGGCGGCCGCGTCGCGTCC
GCCACGCGCGCCGCGACGGG

Product: UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase

Products: NA

Alternate protein names: D-glutamic acid-adding enzyme; UDP-N-acetylmuramoyl-L-alanyl-D-glutamate synthetase [H]

Number of amino acids: Translated: 503; Mature: 503

Protein sequence:

>503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGIELVGLSPGL
SPLEPALAALVAAANEQGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLAAAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAPRTVTFGLNEPVQDGDYGLSRDNGIAWLVEAVDRDAPDEAATTTRRRKRDAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHVTLEGAVHAAADLAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDEIAIDKGATP

Sequences:

>Translated_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGIELVGLSPGL
SPLEPALAALVAAANEQGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLAAAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAPRTVTFGLNEPVQDGDYGLSRDNGIAWLVEAVDRDAPDEAATTTRRRKRDAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHVTLEGAVHAAADLAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDEIAIDKGATP
>Mature_503_residues
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGGAFTPALLDGGIELVGLSPGL
SPLEPALAALVAAANEQGVAVWGELEFFAQALRALGTSGYQPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPA
MLDRLAAAIDETALPDVWVLELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
MKFAPAAGAADAPRTVTFGLNEPVQDGDYGLSRDNGIAWLVEAVDRDAPDEAATTTRRRKRDAHTPDIAQKRLMPADALR
IRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHRVEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAG
GDGKGQDFAPLVAPVARWCRAVMLIGRDAPAIRDTLAETGVPLADHVTLEGAVHAAADLAEPGDAVLLSPACASLDMFRN
YAHRADVFRAAVDEIAIDKGATP

Specific function: Cell wall formation. Catalyzes the addition of glutamate to the nucleotide precursor UDP-N-acetylmuramoyl-L-alanine (UMA) [H]

COG id: COG0771

COG function: function code M; UDP-N-acetylmuramoylalanine-D-glutamate ligase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the MurCDEF family [H]

Homologues:

Organism=Escherichia coli, GI1786276, Length=506, Percent_Identity=35.3754940711462, Blast_Score=219, Evalue=4e-58,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004101
- InterPro:   IPR013221
- InterPro:   IPR016040
- InterPro:   IPR005762 [H]

Pfam domain/function: PF02875 Mur_ligase_C; PF08245 Mur_ligase_M [H]

EC number: =6.3.2.9 [H]

Molecular weight: Translated: 52793; Mature: 52793

Theoretical pI: Translated: 4.94; Mature: 4.94

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.0 %Cys     (Translated Protein)
1.4 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.0 %Cys     (Mature Protein)
1.4 %Met     (Mature Protein)
2.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
AFTPALLDGGIELVGLSPGLSPLEPALAALVAAANEQGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPAMLDRLAAAIDETALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECHHHEECCCCCCHH
MKFAPAAGAADAPRTVTFGLNEPVQDGDYGLSRDNGIAWLVEAVDRDAPDEAATTTRRRK
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHH
RDAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPAIRDTLAETGVPLADHVTLEGAVHAAADLAEPGDAVLLSPACASLDMFRN
HHHHHCCCCHHHHHHHHHCCCCCHHCEEEHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
YAHRADVFRAAVDEIAIDKGATP
HHHHHHHHHHHHHHHHCCCCCCC
>Mature Secondary Structure
MFGDRQRPMVLVLGLGESGLAIARWCARHGCRLRIADTREAPPNLAALQAEGIDAEFVGG
CCCCCCCCEEEEEECCCCCHHHHHHHHHCCCEEEECCCCCCCCCCEEEEECCCCCCCCCC
AFTPALLDGGIELVGLSPGLSPLEPALAALVAAANEQGVAVWGELEFFAQALRALGTSGY
CCCHHHHCCCEEEEEECCCCCHHHHHHHHHHHHCCCCCEEEEECHHHHHHHHHHHCCCCC
QPKVLAITGTNGKTTTTSLTGLLCQRSGKKVAVAGNISPAMLDRLAAAIDETALPDVWVL
CCEEEEEECCCCCEEHHHHHHHHHHCCCCEEEEECCCCHHHHHHHHHHHHHCCCCCEEEE
ELSSFQLETARTFAPDAAAILNITQDHLDWHGSFDAYAQAKGRIFGATTTRVLNRDDAAV
EECCCEEHHHHHCCCCHHHHHHHHHHHCCCCCCCHHHHHCCCEEEECHHHEECCCCCCHH
MKFAPAAGAADAPRTVTFGLNEPVQDGDYGLSRDNGIAWLVEAVDRDAPDEAATTTRRRK
EEECCCCCCCCCCCEEEECCCCCCCCCCCCCCCCCCCEEEEEHHCCCCCCHHHHHHHHHH
RDAHTPDIAQKRLMPADALRIRGLHNAANALAAFALARAIDLPAAPLLHALREYRGEAHR
CCCCCCCHHHHHCCCHHHHHHCCHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHCCCCEE
VEVIATIDDVDYVDDSKGTNVGATVAALDGLAQKIVLIAGGDGKGQDFAPLVAPVARWCR
EEEEEEECCCCCCCCCCCCCCCHHHHHHHHHCEEEEEEECCCCCCCCCHHHHHHHHHHHH
AVMLIGRDAPAIRDTLAETGVPLADHVTLEGAVHAAADLAEPGDAVLLSPACASLDMFRN
HHHHHCCCCHHHHHHHHHCCCCCHHCEEEHHHHHHHHHCCCCCCEEEECCHHHHHHHHHH
YAHRADVFRAAVDEIAIDKGATP
HHHHHHHHHHHHHHHHCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: NA