BacMap

Definition	Burkholderia sp. 383 chromosome 1, complete genome.
Accession	NC_007510
Length	3,694,126

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The map label for this gene is paaG [H]

Identifier: 78065104

GI number: 78065104

Start: 502056

End: 502847

Strand: Reverse

Name: paaG [H]

Synonym: Bcep18194_A3628

Alternate gene names: 78065104

Gene position: 502847-502056 (Counterclockwise)

Preceding gene: 78065105

Following gene: 78065103

Centisome position: 13.61

GC content: 66.41

Gene sequence:

>792_bases
ATGTCCTATCAGGCGATTCAGCTGGATATCGATCACGCGTCGAACGTGGCCACGATCACCCTCAACCGCCCCGACAAGCT
GAACAGCTTTACGCGGGCGATGCATCGCGAGCTGCAGTCGGCACTCGATGATGTCGAGGCGGCCGGCGCGCGCGCGCTGA
TCCTGACAGGTGCGGGACGCGGCTTCTGCGCCGGCCAGGACCTGGCCGATCTCGACTTCACGCCGGGCGCGTCTACCGAC
CTCGGCACGCTGATCGACGAACACTTCAATCCACTGATCCGCCGCCTGCAGCGTCTGCCGATTCCGGTGATCGCCGCCGT
CAATGGTACGGCGGCCGGCGCCGGTGCGAATCTCGCGCTCGCCTGCGATCTCGTGTTCGCCGCCCGCTCGAGCAGTTTCA
TCCAGGCCTTCGTCAAGATCGGGCTCGTACCCGATTCCGGCGGTACGTGGTTCCTGCCGCAACGCGTCGGCATGGCACGC
GCGCTGGGGCTCGCACTGACCGGCGACAAGCTCGGGGCCGAACAGGCCGAGCAATGGGGCTTGATCTGGCGTGCCGTCGA
CGACGACACGCTCGCTGCGACAGCCCGCCAGCTCGCCACCCAGCTCGCGCAGCAGCCGACGCTTGCGATCGCATCGATCA
AGCAATCGATGCGCGCCAGTGTCACGAACACGCTCGACCAGCAACTCGACATGGAACGCGACCTGCAGCGCACGCTCGGC
CAGTCGCACGACTACGCGGAAGGCGTGAAAGCATTCATCGAGAAGCGCGCGCCGCGCTTCGAGGGGCGTTGA

Upstream 100 bases:

>100_bases
TACGATGTGCATCGGCGTCGGCCAGGGCATCGCACTCGCGATCGAACGCGTGTAACCCACGCACCGCGCGCCTATAACGA
AGTCATGAAGGAGATACTGC

Downstream 100 bases:

>100_bases
CATGACAAATGCCACTGCCACGCTCGACCCCGATTCGCTAGCCCGCGCCACCGCACGGGCCATGTACGAAGCCGACGCAT
GCAGCCGCGCGTTCGGGATG

Product: enoyl-CoA hydratase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 263; Mature: 262

Protein sequence:

>263_residues
MSYQAIQLDIDHASNVATITLNRPDKLNSFTRAMHRELQSALDDVEAAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDTLAATARQLATQLAQQPTLAIASIKQSMRASVTNTLDQQLDMERDLQRTLG
QSHDYAEGVKAFIEKRAPRFEGR

Sequences:

>Translated_263_residues
MSYQAIQLDIDHASNVATITLNRPDKLNSFTRAMHRELQSALDDVEAAGARALILTGAGRGFCAGQDLADLDFTPGASTD
LGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMAR
ALGLALTGDKLGAEQAEQWGLIWRAVDDDTLAATARQLATQLAQQPTLAIASIKQSMRASVTNTLDQQLDMERDLQRTLG
QSHDYAEGVKAFIEKRAPRFEGR
>Mature_262_residues
SYQAIQLDIDHASNVATITLNRPDKLNSFTRAMHRELQSALDDVEAAGARALILTGAGRGFCAGQDLADLDFTPGASTDL
GTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLALACDLVFAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARA
LGLALTGDKLGAEQAEQWGLIWRAVDDDTLAATARQLATQLAQQPTLAIASIKQSMRASVTNTLDQQLDMERDLQRTLGQ
SHDYAEGVKAFIEKRAPRFEGR

Specific function: Could possibly oxidize fatty acids using specific components [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the enoyl-CoA hydratase/isomerase family [H]

Homologues:

Organism=Homo sapiens, GI194097323, Length=249, Percent_Identity=33.7349397590361, Blast_Score=138, Evalue=4e-33,
Organism=Homo sapiens, GI70995211, Length=267, Percent_Identity=28.8389513108614, Blast_Score=101, Evalue=6e-22,
Organism=Homo sapiens, GI31542718, Length=253, Percent_Identity=28.0632411067194, Blast_Score=91, Evalue=9e-19,
Organism=Homo sapiens, GI4502327, Length=253, Percent_Identity=29.6442687747036, Blast_Score=84, Evalue=2e-16,
Organism=Homo sapiens, GI157694516, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI213417737, Length=237, Percent_Identity=27.4261603375527, Blast_Score=82, Evalue=4e-16,
Organism=Homo sapiens, GI221136756, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI221136753, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=1e-15,
Organism=Homo sapiens, GI221307494, Length=238, Percent_Identity=26.0504201680672, Blast_Score=80, Evalue=2e-15,
Organism=Homo sapiens, GI45643119, Length=208, Percent_Identity=27.4038461538462, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260275230, Length=208, Percent_Identity=27.4038461538462, Blast_Score=79, Evalue=3e-15,
Organism=Homo sapiens, GI260274832, Length=208, Percent_Identity=27.4038461538462, Blast_Score=79, Evalue=4e-15,
Organism=Homo sapiens, GI20127408, Length=179, Percent_Identity=28.4916201117318, Blast_Score=79, Evalue=5e-15,
Organism=Homo sapiens, GI25453481, Length=243, Percent_Identity=23.8683127572016, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI51538573, Length=243, Percent_Identity=23.8683127572016, Blast_Score=78, Evalue=6e-15,
Organism=Homo sapiens, GI4757968, Length=243, Percent_Identity=23.8683127572016, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI49355787, Length=243, Percent_Identity=23.8683127572016, Blast_Score=78, Evalue=8e-15,
Organism=Homo sapiens, GI37594469, Length=242, Percent_Identity=27.6859504132231, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI37594471, Length=235, Percent_Identity=28.5106382978723, Blast_Score=75, Evalue=5e-14,
Organism=Homo sapiens, GI4757966, Length=237, Percent_Identity=24.0506329113924, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI51538581, Length=237, Percent_Identity=24.0506329113924, Blast_Score=75, Evalue=6e-14,
Organism=Homo sapiens, GI68989263, Length=192, Percent_Identity=29.1666666666667, Blast_Score=73, Evalue=2e-13,
Organism=Homo sapiens, GI116235487, Length=202, Percent_Identity=25.2475247524752, Blast_Score=73, Evalue=3e-13,
Organism=Homo sapiens, GI157694520, Length=193, Percent_Identity=27.979274611399, Blast_Score=65, Evalue=5e-11,
Organism=Escherichia coli, GI1787660, Length=250, Percent_Identity=58.8, Blast_Score=270, Evalue=5e-74,
Organism=Escherichia coli, GI1787659, Length=261, Percent_Identity=35.632183908046, Blast_Score=142, Evalue=2e-35,
Organism=Escherichia coli, GI221142681, Length=271, Percent_Identity=35.7933579335793, Blast_Score=123, Evalue=1e-29,
Organism=Escherichia coli, GI87082183, Length=267, Percent_Identity=28.4644194756554, Blast_Score=111, Evalue=5e-26,
Organism=Escherichia coli, GI1788597, Length=249, Percent_Identity=28.5140562248996, Blast_Score=82, Evalue=3e-17,
Organism=Escherichia coli, GI1788682, Length=193, Percent_Identity=30.5699481865285, Blast_Score=73, Evalue=1e-14,
Organism=Escherichia coli, GI1790281, Length=205, Percent_Identity=28.780487804878, Blast_Score=69, Evalue=4e-13,
Organism=Caenorhabditis elegans, GI25145438, Length=249, Percent_Identity=35.7429718875502, Blast_Score=155, Evalue=2e-38,
Organism=Caenorhabditis elegans, GI17554946, Length=255, Percent_Identity=31.3725490196078, Blast_Score=134, Evalue=7e-32,
Organism=Caenorhabditis elegans, GI17536985, Length=265, Percent_Identity=28.6792452830189, Blast_Score=104, Evalue=4e-23,
Organism=Caenorhabditis elegans, GI17560910, Length=245, Percent_Identity=27.7551020408163, Blast_Score=103, Evalue=1e-22,
Organism=Caenorhabditis elegans, GI17549921, Length=235, Percent_Identity=28.936170212766, Blast_Score=99, Evalue=1e-21,
Organism=Caenorhabditis elegans, GI17540306, Length=267, Percent_Identity=29.9625468164794, Blast_Score=94, Evalue=6e-20,
Organism=Caenorhabditis elegans, GI17535521, Length=261, Percent_Identity=24.904214559387, Blast_Score=90, Evalue=1e-18,
Organism=Caenorhabditis elegans, GI17534483, Length=225, Percent_Identity=25.7777777777778, Blast_Score=80, Evalue=1e-15,
Organism=Caenorhabditis elegans, GI17540714, Length=248, Percent_Identity=25.4032258064516, Blast_Score=79, Evalue=2e-15,
Organism=Caenorhabditis elegans, GI25144276, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=4e-12,
Organism=Caenorhabditis elegans, GI17508951, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Caenorhabditis elegans, GI17508953, Length=182, Percent_Identity=28.5714285714286, Blast_Score=68, Evalue=6e-12,
Organism=Saccharomyces cerevisiae, GI6320241, Length=191, Percent_Identity=27.2251308900524, Blast_Score=72, Evalue=6e-14,
Organism=Drosophila melanogaster, GI20129971, Length=261, Percent_Identity=31.4176245210728, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653477, Length=261, Percent_Identity=31.4176245210728, Blast_Score=141, Evalue=4e-34,
Organism=Drosophila melanogaster, GI24653139, Length=253, Percent_Identity=30.8300395256917, Blast_Score=107, Evalue=6e-24,
Organism=Drosophila melanogaster, GI19922422, Length=259, Percent_Identity=26.2548262548263, Blast_Score=95, Evalue=6e-20,
Organism=Drosophila melanogaster, GI19920382, Length=255, Percent_Identity=29.8039215686275, Blast_Score=94, Evalue=7e-20,
Organism=Drosophila melanogaster, GI21357171, Length=228, Percent_Identity=29.8245614035088, Blast_Score=90, Evalue=1e-18,
Organism=Drosophila melanogaster, GI24654903, Length=216, Percent_Identity=26.8518518518519, Blast_Score=90, Evalue=2e-18,
Organism=Drosophila melanogaster, GI28571730, Length=162, Percent_Identity=32.0987654320988, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI28571729, Length=162, Percent_Identity=32.0987654320988, Blast_Score=75, Evalue=4e-14,
Organism=Drosophila melanogaster, GI24650670, Length=262, Percent_Identity=26.3358778625954, Blast_Score=69, Evalue=2e-12,
Organism=Drosophila melanogaster, GI24583077, Length=221, Percent_Identity=28.0542986425339, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI24583079, Length=221, Percent_Identity=28.0542986425339, Blast_Score=65, Evalue=6e-11,
Organism=Drosophila melanogaster, GI19921000, Length=221, Percent_Identity=28.0542986425339, Blast_Score=65, Evalue=6e-11,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001753
- InterPro:   IPR011968 [H]

Pfam domain/function: PF00378 ECH [H]

EC number: =4.2.1.17 [H]

Molecular weight: Translated: 28224; Mature: 28093

Theoretical pI: Translated: 5.70; Mature: 5.70

Prosite motif: PS00166 ENOYL_COA_HYDRATASE

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.8 %Cys     (Translated Protein)
1.9 %Met     (Translated Protein)
2.7 %Cys+Met (Translated Protein)
0.8 %Cys     (Mature Protein)
1.5 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSYQAIQLDIDHASNVATITLNRPDKLNSFTRAMHRELQSALDDVEAAGARALILTGAGR
CCCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GFCAGQDLADLDFTPGASTDLGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ACDLVFAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCCCCHHHHHHHC
LIWRAVDDDTLAATARQLATQLAQQPTLAIASIKQSMRASVTNTLDQQLDMERDLQRTLG
EEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSHDYAEGVKAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC
>Mature Secondary Structure 
SYQAIQLDIDHASNVATITLNRPDKLNSFTRAMHRELQSALDDVEAAGARALILTGAGR
CCEEEEEECCCCCCEEEEEECCCHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEEECCC
GFCAGQDLADLDFTPGASTDLGTLIDEHFNPLIRRLQRLPIPVIAAVNGTAAGAGANLAL
CCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHCCCCEEEEECCCCCCCCCCHHH
ACDLVFAARSSSFIQAFVKIGLVPDSGGTWFLPQRVGMARALGLALTGDKLGAEQAEQWG
HHHHHHHHCCHHHHHHHHHHCCEECCCCCEECHHHHHHHHHHHHEECCCCCCHHHHHHHC
LIWRAVDDDTLAATARQLATQLAQQPTLAIASIKQSMRASVTNTLDQQLDMERDLQRTLG
EEEEECCCHHHHHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHC
QSHDYAEGVKAFIEKRAPRFEGR
CCCCHHHHHHHHHHHCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9097039; 9278503; 10766858 [H]