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The map label for this gene is paaI [H]

Identifier: 78065103

GI number: 78065103

Start: 501602

End: 502054

Strand: Reverse

Name: paaI [H]

Synonym: Bcep18194_A3627

Alternate gene names: 78065103

Gene position: 502054-501602 (Counterclockwise)

Preceding gene: 78065104

Following gene: 78065102

Centisome position: 13.59

GC content: 64.68

Gene sequence:

>453_bases
ATGACAAATGCCACTGCCACGCTCGACCCCGATTCGCTAGCCCGCGCCACCGCACGGGCCATGTACGAAGCCGACGCATG
CAGCCGCGCGTTCGGGATGGAAATCGCCGAAGTACGTGCCGGCTACGCGCGTCTGCAGATGCGCGTGCGTCCCGAGTTCC
TGAACGGTCATCAGACCTGCCATGGCGGAATCATCTTCACGCTGGCCGATTCGACGTTCGCGTTCGCGTGCAACTCGTAC
AACGTGAATACCGTCGCGGCCGGCTGCTCGATCGAATTCCTGCGCCCGGTGCACGGCGACGACGTGCTGACGGCCGAGGC
GATCGAGCAAGCGCGCGCCGGCCGCCAAGGCATCTACGACATCCGCGTCACGAACCAGACCGGCGAAACCGTCGCGATGT
TTCGCGGCAAATCCGCCCAGATCAAGGGCACGGTCATCCCGGAAGACCGCTGA

Upstream 100 bases:

>100_bases
GAACGCGACCTGCAGCGCACGCTCGGCCAGTCGCACGACTACGCGGAAGGCGTGAAAGCATTCATCGAGAAGCGCGCGCC
GCGCTTCGAGGGGCGTTGAC

Downstream 100 bases:

>100_bases
CGTCGACGGCTCGCACATAACAAACACTGGAGACATGCATGACTACCCCGCTACCGCTCGAGCCGATCGAGACCGCCTCA
CGCGACGAGCTGACCGCGCT

Product: phenylacetic acid degradation protein PaaD

Products: NA

Alternate protein names: Phenylacetic acid degradation protein PaaI [H]

Number of amino acids: Translated: 150; Mature: 149

Protein sequence:

>150_residues
MTNATATLDPDSLARATARAMYEADACSRAFGMEIAEVRAGYARLQMRVRPEFLNGHQTCHGGIIFTLADSTFAFACNSY
NVNTVAAGCSIEFLRPVHGDDVLTAEAIEQARAGRQGIYDIRVTNQTGETVAMFRGKSAQIKGTVIPEDR

Sequences:

>Translated_150_residues
MTNATATLDPDSLARATARAMYEADACSRAFGMEIAEVRAGYARLQMRVRPEFLNGHQTCHGGIIFTLADSTFAFACNSY
NVNTVAAGCSIEFLRPVHGDDVLTAEAIEQARAGRQGIYDIRVTNQTGETVAMFRGKSAQIKGTVIPEDR
>Mature_149_residues
TNATATLDPDSLARATARAMYEADACSRAFGMEIAEVRAGYARLQMRVRPEFLNGHQTCHGGIIFTLADSTFAFACNSYN
VNTVAAGCSIEFLRPVHGDDVLTAEAIEQARAGRQGIYDIRVTNQTGETVAMFRGKSAQIKGTVIPEDR

Specific function: Thioesterase with a preference for ring-hydroxylated phenylacetyl-CoA esters. Hydrolyzes 3,4-dihydroxyphenylacetyl-CoA, 3-hydroxyphenylacetyl-CoA and 4-hydroxyphenylacetyl-CoA. Inactive towards 4-hydroxybenzoyl-CoA and 4-hydroxyphenacyl-CoA [H]

COG id: COG2050

COG function: function code Q; Uncharacterized protein, possibly involved in aromatic compounds catabolism

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the thioesterase PaaI family [H]

Homologues:

Organism=Escherichia coli, GI1787662, Length=135, Percent_Identity=48.8888888888889, Blast_Score=141, Evalue=1e-35,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR011973
- InterPro:   IPR003736
- InterPro:   IPR006683 [H]

Pfam domain/function: PF03061 4HBT [H]

EC number: NA

Molecular weight: Translated: 16251; Mature: 16120

Theoretical pI: Translated: 6.25; Mature: 6.25

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.7 %Cys     (Translated Protein)
3.3 %Met     (Translated Protein)
6.0 %Cys+Met (Translated Protein)
2.7 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
5.4 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTNATATLDPDSLARATARAMYEADACSRAFGMEIAEVRAGYARLQMRVRPEFLNGHQTC
CCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHEEEEEECHHHCCCCCEE
HGGIIFTLADSTFAFACNSYNVNTVAAGCSIEFLRPVHGDDVLTAEAIEQARAGRQGIYD
CCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCCEEHHHHHHHHHCCCCCEEE
IRVTNQTGETVAMFRGKSAQIKGTVIPEDR
EEEECCCCCEEEEEECCCCEEEEEECCCCC
>Mature Secondary Structure 
TNATATLDPDSLARATARAMYEADACSRAFGMEIAEVRAGYARLQMRVRPEFLNGHQTC
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCHHHHHHHCHHEEEEEECHHHCCCCCEE
HGGIIFTLADSTFAFACNSYNVNTVAAGCSIEFLRPVHGDDVLTAEAIEQARAGRQGIYD
CCCEEEEEECCEEEEEECCCCCEEEEECCEEEEECCCCCCCEEHHHHHHHHHCCCCCEEE
IRVTNQTGETVAMFRGKSAQIKGTVIPEDR
EEEECCCCCEEEEEECCCCEEEEEECCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 9748275; 9278503; 10766858 [H]