Click here to switch to the map view.

The map label for this gene is gcvA [H]

Identifier: 78065082

GI number: 78065082

Start: 478674

End: 479591

Strand: Reverse

Name: gcvA [H]

Synonym: Bcep18194_A3606

Alternate gene names: 78065082

Gene position: 479591-478674 (Counterclockwise)

Preceding gene: 78065087

Following gene: 78065081

Centisome position: 12.98

GC content: 70.92

Gene sequence:

>918_bases
ATGAATCGTTCCCGCCTACCGCCTCTCAACGCGCTGCGCGCCTTCGAAGCAGCTGCCCGCCACCTGTCGGTGAAAGCGGC
TGCCGAGGAGCTGTCGGTCACGCCCGGCGCGGTGAGCCAGATGATCCGCACGCTCGAGACGCATCTCGGCGTGCAGCTGT
TCGAGCGCGTCAACCGCGGCATCCTGCTCACGGCCGCCGGCCGCGACTACCTGCCGCCGGTGCGCAACGCATTCAGGCAG
ATCGCCGATGCGTCGCAGCGCGTGTCGGGTGCTGCCGACAGCGGCGTGCTGACGGTGAGCGTCACGCCTTTCTTCGCATC
CGCGTGGCTCGTCCCGCGCCTCGCGCAGTTCCGGGAGGCCTGCCCTGACGTCGACCTGCAGGTCGTCACGAGCCACGCGC
TCGCGGATTTCTCGCGCGATGGCGTCGACGTCGCGATCCGTCACGGCCTCGGGCGCTACATCGGGCTGTGCAGCGAGCGC
CTGTTGACGGTGGAGATCGTCGCGCTCGCGTCGCCCGAACTCGTCGCGCGGCTCGGCATGCCGGCGACGCCCGCCGATCT
CGTCGGCTGGCCGCAGCTGCACGACGCCGAGCGCAAGGGCTGGCACATCTGGTTCGGCGCGCAGCGGATCGACGATTTCG
GCCCGCCGCGCGGCCCCGCGTTCGACGATTCGGGCCTGTTGCTGCAGGCGATCGTCGCCGGCCAGGGCGCCGGGCTGCTG
CCGGCCGCGATGGTGCGCGGCGAGCTGGCAAGCGGCCGGCTCGTGCAGCTCGCCGACGTCGCGTGGCTCGACGATTTCGC
GTACTACCTCGTCTATCCGCCGCATCACGCGGAACGGCCAAAGGTCGCGGCATTCCGTCGATGGATTCTCGACGCCGCAC
TGGCCGACGGCGCGGCATCGATGTCCGGCGCGTCGTAG

Upstream 100 bases:

>100_bases
CAGGCAGTCGCGAAGCATGTGATCCAATGCGTCAACGGTACTCGCGCGTACACCTCGTCTCAAACGATATTTCGACGGAC
TTTCGATTTAGAAAAACTAA

Downstream 100 bases:

>100_bases
CGCCACTCCCCCGGCGCGGCGCGGTTTCTCTGCCGATCGGCTAACATGCGGCCCGGCACCGATAACAACAGACCGAGAGA
GACCATGCCTGCCCTGCTCC

Product: LysR family transcriptional regulator

Products: NA

Alternate protein names: Gcv operon activator [H]

Number of amino acids: Translated: 305; Mature: 305

Protein sequence:

>305_residues
MNRSRLPPLNALRAFEAAARHLSVKAAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFREACPDVDLQVVTSHALADFSRDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPELVARLGMPATPADLVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAALADGAASMSGAS

Sequences:

>Translated_305_residues
MNRSRLPPLNALRAFEAAARHLSVKAAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFREACPDVDLQVVTSHALADFSRDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPELVARLGMPATPADLVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAALADGAASMSGAS
>Mature_305_residues
MNRSRLPPLNALRAFEAAARHLSVKAAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRGILLTAAGRDYLPPVRNAFRQ
IADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFREACPDVDLQVVTSHALADFSRDGVDVAIRHGLGRYIGLCSER
LLTVEIVALASPELVARLGMPATPADLVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAALADGAASMSGAS

Specific function: Regulatory protein for the glycine cleavage system operon (gcv). Mediates activation of gcv by glycine and repression by purines. GcvA is negatively autoregulated. Bind to three sites upstream of the gcv promoter [H]

COG id: COG0583

COG function: function code K; Transcriptional regulator

Gene ontology:

Cell location: Cytoplasmic [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 HTH lysR-type DNA-binding domain [H]

Homologues:

Organism=Escherichia coli, GI1789173, Length=293, Percent_Identity=41.6382252559727, Blast_Score=217, Evalue=7e-58,
Organism=Escherichia coli, GI1786448, Length=289, Percent_Identity=34.2560553633218, Blast_Score=139, Evalue=2e-34,
Organism=Escherichia coli, GI1788706, Length=289, Percent_Identity=31.4878892733564, Blast_Score=125, Evalue=4e-30,
Organism=Escherichia coli, GI145693193, Length=287, Percent_Identity=27.1777003484321, Blast_Score=100, Evalue=2e-22,
Organism=Escherichia coli, GI1786401, Length=267, Percent_Identity=29.2134831460674, Blast_Score=94, Evalue=1e-20,
Organism=Escherichia coli, GI1787128, Length=272, Percent_Identity=26.1029411764706, Blast_Score=90, Evalue=2e-19,
Organism=Escherichia coli, GI87081978, Length=266, Percent_Identity=32.3308270676692, Blast_Score=82, Evalue=5e-17,
Organism=Escherichia coli, GI1789639, Length=250, Percent_Identity=27.6, Blast_Score=80, Evalue=1e-16,
Organism=Escherichia coli, GI1787879, Length=303, Percent_Identity=27.3927392739274, Blast_Score=78, Evalue=7e-16,
Organism=Escherichia coli, GI1787589, Length=279, Percent_Identity=24.7311827956989, Blast_Score=76, Evalue=3e-15,
Organism=Escherichia coli, GI157672245, Length=137, Percent_Identity=36.4963503649635, Blast_Score=69, Evalue=4e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000847
- InterPro:   IPR005119
- InterPro:   IPR011991 [H]

Pfam domain/function: PF00126 HTH_1; PF03466 LysR_substrate [H]

EC number: NA

Molecular weight: Translated: 32795; Mature: 32795

Theoretical pI: Translated: 6.96; Mature: 6.96

Prosite motif: PS50931 HTH_LYSR

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.7 %Cys     (Translated Protein)
1.6 %Met     (Translated Protein)
2.3 %Cys+Met (Translated Protein)
0.7 %Cys     (Mature Protein)
1.6 %Met     (Mature Protein)
2.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKAAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFREA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
CPDVDLQVVTSHALADFSRDGVDVAIRHGLGRYIGLCSERLLTVEIVALASPELVARLGM
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHCCC
PATPADLVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
CCCCHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCH
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAALADGAAS
HHHHHHHHCCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
MSGAS
CCCCC
>Mature Secondary Structure
MNRSRLPPLNALRAFEAAARHLSVKAAAEELSVTPGAVSQMIRTLETHLGVQLFERVNRG
CCCCCCCCHHHHHHHHHHHHHHHHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHHHHHCCC
ILLTAAGRDYLPPVRNAFRQIADASQRVSGAADSGVLTVSVTPFFASAWLVPRLAQFREA
EEEEECCCCCCCHHHHHHHHHHHHHHHHCCCCCCCEEEEEECHHHHHHHHHHHHHHHHHH
CPDVDLQVVTSHALADFSRDGVDVAIRHGLGRYIGLCSERLLTVEIVALASPELVARLGM
CCCCCHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHHHHHEEEEEEECCCHHHHHHCCC
PATPADLVGWPQLHDAERKGWHIWFGAQRIDDFGPPRGPAFDDSGLLLQAIVAGQGAGLL
CCCCHHHCCCCCCCCCCCCCEEEEECHHHHHHCCCCCCCCCCCCCHHHHHHHHCCCCCCH
PAAMVRGELASGRLVQLADVAWLDDFAYYLVYPPHHAERPKVAAFRRWILDAALADGAAS
HHHHHHHHCCCCCEEEHHHHHHHHCCEEEEEECCCCCCCCHHHHHHHHHHHHHHHCCCCC
MSGAS
CCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: DNA [C]

Specific reaction: Protein + DNA = Protein-DNA [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]