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The map label for this gene is mlaE [H]

Identifier: 78064990

GI number: 78064990

Start: 381996

End: 382763

Strand: Direct

Name: mlaE [H]

Synonym: Bcep18194_A3513

Alternate gene names: 78064990

Gene position: 381996-382763 (Clockwise)

Preceding gene: 78064989

Following gene: 78064991

Centisome position: 10.34

GC content: 66.02

Gene sequence:

>768_bases
ATGATCAGCGCGATCGGACGTTACGTCATCGGCGGCCTCGAGCGCACGGGCTACGGCACGCGGCTGTTCGTGCGCCTCGT
GCTGGAATTCTTCCCGCTGCTGCGCCGGCCGCGGTTGGTCACGAAGCAGATCCACTTCCTCGGCAACTATTCGTTCGTGA
TCATTGCCGTGTCGGGGCTGTTCGTCGGCTTCGTGCTCGGCCTGCAGGGGTATTACACGCTGAACCGCTATGGTTCCGAG
CAGGCGCTCGGCCTGCTGGTCGCGCTGTCGCTGGTGCGTGAGCTGGGGCCGGTCGTCACCGCGCTGCTGTTCGCGGGCCG
CGCGGGCACGTCGCTCACGGCCGAGATCGGTCTGATGAAAGCCGGCGAGCAGCTCACCGCGCTCGAAATGATGGCGGTGG
ACCCGATCAAGAACGTGATCGCGCCGCGCATGTGGGCGGGCATCATCGCGATGCCGCTGCTGGCCGCGATCTTCAACGCG
GTCGGCGTGCTCGGCGGCTACTTCGTCGGCGTCGTGCTGATCGGCGTCGATCCGGGGGCGTTCTGGTCGCAGATGCAGGG
CGGCGTGCAGGTCTGGGCCGACGTCGGCAACGGCGTGCTGAAGAGCATCGTGTTCGGGTTCGCCGTGACCTTCATTGCGC
TGTATCAAGGGTATGAAGCGAAGCCCACGCCTGAGGGCGTGTCGCGCGCGACCACCAAGACGGTCGTGTTCGCGTCGCTC
GCCGTACTCGGCCTCGATTTCCTGCTGACCGCGCTGATGTTCAGCTAA

Upstream 100 bases:

>100_bases
GCGTGCGGCAGTTCATCGACGGCGCGCCGGACGGCCCGTTCAAATTTCATTACCAGAGCCCGCCGCTCGCAGCGGATTTC
GGGCTCGGCGGAGGGCGCGC

Downstream 100 bases:

>100_bases
GCCTCAGCCAAGCCAAATTTTGGGATGACGATGAAAAAGACTGCTCTCGACTTCTGGGTCGGCCTGTTCGTGGTGGTGGG
CTTCCTTGCGGTGCTGTTCC

Product: ABC transporter, inner membrane subunit

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 255; Mature: 255

Protein sequence:

>255_residues
MISAIGRYVIGGLERTGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVLKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Sequences:

>Translated_255_residues
MISAIGRYVIGGLERTGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVLKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS
>Mature_255_residues
MISAIGRYVIGGLERTGYGTRLFVRLVLEFFPLLRRPRLVTKQIHFLGNYSFVIIAVSGLFVGFVLGLQGYYTLNRYGSE
QALGLLVALSLVRELGPVVTALLFAGRAGTSLTAEIGLMKAGEQLTALEMMAVDPIKNVIAPRMWAGIIAMPLLAAIFNA
VGVLGGYFVGVVLIGVDPGAFWSQMQGGVQVWADVGNGVLKSIVFGFAVTFIALYQGYEAKPTPEGVSRATTKTVVFASL
AVLGLDFLLTALMFS

Specific function: Part of the ABC transporter complex mlaFEDB that actively prevents phospholipid accumulation at the cell surface. Probably maintains lipid asymmetry in the outer membrane by retrograde trafficking of phospholipids from the outer membrane to the inner memb

COG id: COG0767

COG function: function code Q; ABC-type transport system involved in resistance to organic solvents, permease component

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the mlaE permease family [H]

Homologues:

Organism=Escherichia coli, GI1789585, Length=220, Percent_Identity=60.4545454545455, Blast_Score=266, Evalue=1e-72,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003453 [H]

Pfam domain/function: PF02405 DUF140 [H]

EC number: NA

Molecular weight: Translated: 27262; Mature: 27262

Theoretical pI: Translated: 9.88; Mature: 9.88

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
3.1 %Met     (Translated Protein)
3.1 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
3.1 %Met     (Mature Protein)
3.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHCC
>Mature Secondary Structure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HHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 7.0

TargetDB status: NA

Availability: NA

References: 11206551; 11258796 [H]