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The map label for this gene is rep [H]

Identifier: 78064806

GI number: 78064806

Start: 176781

End: 178868

Strand: Direct

Name: rep [H]

Synonym: Bcep18194_A3329

Alternate gene names: 78064806

Gene position: 176781-178868 (Clockwise)

Preceding gene: 78064797

Following gene: 78064808

Centisome position: 4.79

GC content: 66.48

Gene sequence:

>2088_bases
ATGTCCGCAGGCCTGAATCCCGCCCAGAACGAAGCGGTGCGCTACCTCGACGGTCCCTGTCTCGTGCTCGCCGGCGCGGG
CAGCGGCAAGACCCGCGTGATCACGCAGAAGATCGCGCACCTGATCGAAGCGAAAGGCTTCGAGCCGCGCCACATCGCCG
CCGTCACGTTCACGAACAAGGCGGCCGCCGAAATGCGCGAGCGCGTGTCGAAGCTGCTCGAGGGCAAGACGCTCACCACG
CCCGGCAAGGAAGGCCGCAAGGTGCCCGTCAACCAGCTCACCGTCTGCACGTTCCACTCGCTGGGCGTGCAGATCCTGCG
CCAGGAGGCCGAGCACGTCGGCCTGAAGCCGCAGTTCTCGATCATGGATTCGGACGACTGCTTCGGGATGATCCAGGAGC
AGATCGGCACGACCGACAAGGGCCTGATCCGCAAGATCCAGAGCACCATCTCGCTGTGGAAGAACGGCCTGATCATGCCC
GACGAGGCGATGACGATCGCGGCCAACGAGGACGAGCACCAGGCCGCGCTGGTCTACCGCAACTACGTCGCGACGCTGCA
TGCGTACCAGGCGGTCGACTTCGACGACCTGATCCGCCTGCCCGCCGAGCTGTTCGCGAAGAACGAGCAGGTGCGCGACC
GCTGGCAGAACAAGCTGCGCTATCTGCTGATCGACGAGTACCAGGACACCAACGCGTGCCAGTACGAGCTGCTGAAGCTG
CTCGCCGGCCCGCGCGCGGCGTTCACGGCGGTGGGCGACGACGACCAGGCGATCTACGGCTGGCGCGGCGCGACGCTCGA
GAACCTCGCGCAGCTCGGCAAGGATTTCCCGAAGCTGCACCTGGTCAAGCTCGAGCAGAACTACCGGTCGACGGTGCGCA
TCCTGACCGCCGCGAACAACGTGATCGCGAACAACCCGAAACTGTTCGAGAAGAAGCTGTGGTCCGAGCACGGGATGGGC
GATTCGATCACCGTCACGCCGTGCAACGACGAAGAGCATGAAGCCGAGTCGGTCGTGTTCCGGCTGTCCGCGCACAAGTT
CGAGCGGCGCGCGCAGTTCCGCGACTACGCGATCCTGTATCGCGGCAACTTCCAGGCGCGGATCTTCGAGCAGGTGCTGC
GGCGCGAGCGGATCCCGTACGTGCTGTCCGGCGGCCAGTCGTTCTTCGACAAGGCCGAGATCAAGGATCTGTGCGCGTAC
CTGCGGCTGATCGCGAACGCCGACGACGATCCCGCGTTCATCCGCGCGGTCACCACGCCGCGCCGCGGGATCGGCAACAC
GACGCTCGAAGCGCTCGGCTCGTTCGCGGGCCAGGCGAAGGTGTCGCTGTTCGAGGCCGTGTACATGGGCGGGATCGAGG
CGCGGCTGTCCGCGCGCCAGGTCGAGCCGCTGCGGATGTTCTGCGACTTCATCCAGCGCCTGACCGAGCGCGCGGACAAG
GATCCTGCCACCGCCGTGCTCGACGACATGATGGAGGCGATCCACTACGAGGCGTACCTGTATGACGCGTTCGACGAACG
GCAGGCGCAGTCGAAGTGGCAGAACGTGCTCGAATTTCTCGAATGGCTGAAGCGCAAGGGCACCAAGCCCGAGACGGCGG
CCGTCGACGGCGAGGCGGAAGGCTTCCACAACGCGGACGGCCTCGCCGATACGGGCAAGAACCTGCTCGGCCTGATCCAG
ACCGTCGCGCTGATGTCGATGCTAGAGGGCAAGGACGAGGACCCGGACGCCGTGCGGCTGTCGACCGTCCATGCGTCGAA
GGGGCTCGAGTATCCGCACGTGTTCCTGGTCGGCGTCGAGGAAGGCATCATGCCGCACCGCGGCGGCTCCGAGGACGACG
GCCCGATCGACAACGAGCGGATCGAGGAGGAGCGCCGGCTGATGTACGTGGCGATCACGCGCGCGCAGCGCAGCCTGCAC
CTGAACTGGTGCAAGAAGCGCAAGCGGGCGCGCGAGACGGTCGTGTGCGAACCGTCGCGCTTCATCCCGGAGATGGGGCT
CGACGAGGCGCCGCCGCCGACGCCGGAAGAGGCGCCGATGTCGCCGAAGGACCGGCTCGCGAGCCTGAAGGCGTTGCTGC
AGAAGTGA

Upstream 100 bases:

>100_bases
TAACATGCGCGTCCTGCCCGCGTGTCCGCCTGCTTCGCCAGCCCGCCGCGCACGGCATCCTCCCGGCGTTCCGCCGTCAA
CCGCAACACCATCCGCCATC

Downstream 100 bases:

>100_bases
TGCCGAGCGTTCCGGTGGAGCGCCGACAAAACGACAAAACAAAACCCCCGCGATGCGTAGGCGTCGCGGGGGTTTTTTAC
TGCACGGCCGGGCCGGTGTT

Product: UvrD/REP helicase

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 695; Mature: 694

Protein sequence:

>695_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQSTISLWKNGLIMP
DEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKL
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHLVKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTPCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
DPATAVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETAAVDGEAEGFHNADGLADTGKNLLGLIQ
TVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK

Sequences:

>Translated_695_residues
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTT
PGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQSTISLWKNGLIMP
DEAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKL
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHLVKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMG
DSITVTPCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAY
LRLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
DPATAVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETAAVDGEAEGFHNADGLADTGKNLLGLIQ
TVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLH
LNWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
>Mature_694_residues
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNKAAAEMRERVSKLLEGKTLTTP
GKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFSIMDSDDCFGMIQEQIGTTDKGLIRKIQSTISLWKNGLIMPD
EAMTIAANEDEHQAALVYRNYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKLL
AGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHLVKLEQNYRSTVRILTAANNVIANNPKLFEKKLWSEHGMGD
SITVTPCNDEEHEAESVVFRLSAHKFERRAQFRDYAILYRGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYL
RLIANADDDPAFIRAVTTPRRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADKD
PATAVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETAAVDGEAEGFHNADGLADTGKNLLGLIQT
VALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVEEGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHL
NWCKKRKRARETVVCEPSRFIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK

Specific function: Rep helicase is a single-stranded DNA-dependent ATPase involved in DNA replication; it can initiate unwinding at a nick in the DNA. It binds to the single-stranded DNA and acts in a progressive fashion along the DNA in the 3' to 5' direction [H]

COG id: COG0210

COG function: function code L; Superfamily I DNA and RNA helicases

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 uvrD-like helicase C-terminal domain [H]

Homologues:

Organism=Escherichia coli, GI48994965, Length=701, Percent_Identity=47.0756062767475, Blast_Score=588, Evalue=1e-169,
Organism=Escherichia coli, GI2367296, Length=665, Percent_Identity=35.187969924812, Blast_Score=354, Evalue=1e-98,
Organism=Escherichia coli, GI1787196, Length=380, Percent_Identity=27.1052631578947, Blast_Score=97, Evalue=3e-21,
Organism=Saccharomyces cerevisiae, GI6322369, Length=724, Percent_Identity=28.1767955801105, Blast_Score=201, Evalue=3e-52,
Organism=Saccharomyces cerevisiae, GI6324477, Length=668, Percent_Identity=24.251497005988, Blast_Score=114, Evalue=5e-26,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR005752
- InterPro:   IPR013986
- InterPro:   IPR014017
- InterPro:   IPR000212
- InterPro:   IPR014016 [H]

Pfam domain/function: PF00580 UvrD-helicase [H]

EC number: =3.6.4.12 [H]

Molecular weight: Translated: 78084; Mature: 77953

Theoretical pI: Translated: 6.02; Mature: 6.02

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.3 %Cys     (Translated Protein)
2.4 %Met     (Translated Protein)
3.7 %Cys+Met (Translated Protein)
1.3 %Cys     (Mature Protein)
2.3 %Met     (Mature Protein)
3.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQIGTTDKGLIRKIQSTISLWKNGLIMPDEAMTIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHLVKLEQNYRSTVRILTAANN
HCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHEEHHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTPCNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPATAVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETAAVDGEAE
CHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
GFHNADGLADTGKNLLGLIQTVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEEC
EGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC
>Mature Secondary Structure 
SAGLNPAQNEAVRYLDGPCLVLAGAGSGKTRVITQKIAHLIEAKGFEPRHIAAVTFTNK
CCCCCCCHHHHHHHCCCCEEEEEECCCCCHHHHHHHHHHHHHHCCCCCCEEEEEEECCH
AAAEMRERVSKLLEGKTLTTPGKEGRKVPVNQLTVCTFHSLGVQILRQEAEHVGLKPQFS
HHHHHHHHHHHHHCCCEECCCCCCCCCCCCCCEEEEHHHHHHHHHHHHHHHHCCCCCCEE
IMDSDDCFGMIQEQIGTTDKGLIRKIQSTISLWKNGLIMPDEAMTIAANEDEHQAALVYR
ECCCCHHHHHHHHHHCCCHHHHHHHHHHHHHHHHCCCCCCCCCEEEECCCCCHHHHHHHH
NYVATLHAYQAVDFDDLIRLPAELFAKNEQVRDRWQNKLRYLLIDEYQDTNACQYELLKL
HHHHHHHHHHCCCHHHHHHCCHHHHHCCHHHHHHHHHCCEEEEEECCCCCCHHHHHHHHH
LAGPRAAFTAVGDDDQAIYGWRGATLENLAQLGKDFPKLHLVKLEQNYRSTVRILTAANN
HCCCCCEEEEECCCCCEEEECCCCCHHHHHHHCCCCCCEEEEEEHHHHHHHEEHHHHCCC
VIANNPKLFEKKLWSEHGMGDSITVTPCNDEEHEAESVVFRLSAHKFERRAQFRDYAILY
HHCCCCHHHHHHHHHHCCCCCEEEEECCCCCHHHHHHHHHHHHHHHHHHHHHHCCEEEEE
RGNFQARIFEQVLRRERIPYVLSGGQSFFDKAEIKDLCAYLRLIANADDDPAFIRAVTTP
ECCCHHHHHHHHHHHCCCCEEEECCHHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCC
RRGIGNTTLEALGSFAGQAKVSLFEAVYMGGIEARLSARQVEPLRMFCDFIQRLTERADK
CCCCCHHHHHHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHCCC
DPATAVLDDMMEAIHYEAYLYDAFDERQAQSKWQNVLEFLEWLKRKGTKPETAAVDGEAE
CHHHHHHHHHHHHHHHHHHEEHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCEECCCCCC
GFHNADGLADTGKNLLGLIQTVALMSMLEGKDEDPDAVRLSTVHASKGLEYPHVFLVGVE
CCCCCCCCHHHHHHHHHHHHHHHHHHHHCCCCCCCCCEEEEEEEHHCCCCCCEEEEEEEC
EGIMPHRGGSEDDGPIDNERIEEERRLMYVAITRAQRSLHLNWCKKRKRARETVVCEPSR
CCCCCCCCCCCCCCCCCHHHHHHHHHEEEEEEEHHHHHHHHHHHHHHHHHCCCEEECCHH
FIPEMGLDEAPPPTPEEAPMSPKDRLASLKALLQK
HCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 7542800 [H]