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The map label for this gene is gcvH [H]

Identifier: 78064801

GI number: 78064801

Start: 171890

End: 172357

Strand: Reverse

Name: gcvH [H]

Synonym: Bcep18194_A3324

Alternate gene names: 78064801

Gene position: 172357-171890 (Counterclockwise)

Preceding gene: 78064802

Following gene: 78064800

Centisome position: 4.67

GC content: 61.75

Gene sequence:

>468_bases
ATGGCGCCCGGCGCAACACCACACCCGGCGCGCCCCGGCGGCGCGTCAGCAAAACGAATAAACCCTTTTAATCAGGAGCT
TCCGATGAGCAACGTCCCGGCCGATCTGAAATACACCGACGAACACGAGTGGATCCGCACCGAAGCAGACGGCACGCTGA
CGGTCGGCATCACCGATCACGCACAGAGCACGCTTGGCGACATCGTGTTCCTCGAGTTGCCGCAAGCCGGCAAGCAGGTG
AAGGCAGGCGACGCCATCGGCGTCGTCGAGTCGGTGAAGGCTGCATCCGACATCTACTCGCCGGTATCGGGCGAGATCAT
CGCGTTCAACCAGGACGCTGCGGACACGCCGGAAGATGTCAATGGCGATCCGTACGAAGCCTGGCTGTTCAAGATCAAGC
TCGCCGCCGGCGCCAATACCGACAACCTGCTCAGCGCCGACGCTTACAAGAAGCTGATCGGCGACTAA

Upstream 100 bases:

>100_bases
AAGAATCTTCCCGCGCGCGTGGTAAAACTGCCGTTCGTGCGCAACGGCAAGGTCCTCGCTGCGTAACGGCCGGGAGGCCC
GCCTCCCGGGAAGCCGGGAG

Downstream 100 bases:

>100_bases
CTTTCTCACTCGTATCGCGCGGGGTAGGTCAGCCGCCCTGCCTCGCGCGAAGCCGGAGCAAGCGTCCAGGCGCACGCTCC
GGCCGATTGCCAAGCATGCG

Product: glycine cleavage H-protein

Products: Proton; NADH; NH3; CO2; 5,10-methylene-THF [C]

Alternate protein names: NA

Number of amino acids: Translated: 155; Mature: 154

Protein sequence:

>155_residues
MAPGATPHPARPGGASAKRINPFNQELPMSNVPADLKYTDEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPQAGKQV
KAGDAIGVVESVKAASDIYSPVSGEIIAFNQDAADTPEDVNGDPYEAWLFKIKLAAGANTDNLLSADAYKKLIGD

Sequences:

>Translated_155_residues
MAPGATPHPARPGGASAKRINPFNQELPMSNVPADLKYTDEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPQAGKQV
KAGDAIGVVESVKAASDIYSPVSGEIIAFNQDAADTPEDVNGDPYEAWLFKIKLAAGANTDNLLSADAYKKLIGD
>Mature_154_residues
APGATPHPARPGGASAKRINPFNQELPMSNVPADLKYTDEHEWIRTEADGTLTVGITDHAQSTLGDIVFLELPQAGKQVK
AGDAIGVVESVKAASDIYSPVSGEIIAFNQDAADTPEDVNGDPYEAWLFKIKLAAGANTDNLLSADAYKKLIGD

Specific function: The glycine cleavage system catalyzes the degradation of glycine. The H protein shuttles the methylamine group of glycine from the P protein to the T protein [H]

COG id: COG0509

COG function: function code E; Glycine cleavage system H protein (lipoate-binding)

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 lipoyl-binding domain [H]

Homologues:

Organism=Homo sapiens, GI49574537, Length=155, Percent_Identity=41.9354838709677, Blast_Score=112, Evalue=1e-25,
Organism=Homo sapiens, GI89057342, Length=155, Percent_Identity=41.2903225806452, Blast_Score=109, Evalue=1e-24,
Organism=Escherichia coli, GI1789271, Length=128, Percent_Identity=60.15625, Blast_Score=158, Evalue=2e-40,
Organism=Caenorhabditis elegans, GI17551294, Length=116, Percent_Identity=46.551724137931, Blast_Score=109, Evalue=4e-25,
Organism=Caenorhabditis elegans, GI17507493, Length=119, Percent_Identity=44.5378151260504, Blast_Score=106, Evalue=5e-24,
Organism=Saccharomyces cerevisiae, GI6319272, Length=145, Percent_Identity=39.3103448275862, Blast_Score=106, Evalue=2e-24,
Organism=Drosophila melanogaster, GI17865652, Length=125, Percent_Identity=42.4, Blast_Score=111, Evalue=2e-25,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR003016
- InterPro:   IPR002930
- InterPro:   IPR017453
- InterPro:   IPR011053 [H]

Pfam domain/function: PF01597 GCV_H [H]

EC number: NA

Molecular weight: Translated: 16389; Mature: 16258

Theoretical pI: Translated: 4.27; Mature: 4.27

Prosite motif: PS00189 LIPOYL

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
1.3 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
0.6 %Met     (Mature Protein)
0.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MAPGATPHPARPGGASAKRINPFNQELPMSNVPADLKYTDEHEWIRTEADGTLTVGITDH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCCCEEEEEECCC
AQSTLGDIVFLELPQAGKQVKAGDAIGVVESVKAASDIYSPVSGEIIAFNQDAADTPEDV
CHHHHCCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
NGDPYEAWLFKIKLAAGANTDNLLSADAYKKLIGD
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure 
APGATPHPARPGGASAKRINPFNQELPMSNVPADLKYTDEHEWIRTEADGTLTVGITDH
CCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCCEECCCCCEEEECCCCEEEEEECCC
AQSTLGDIVFLELPQAGKQVKAGDAIGVVESVKAASDIYSPVSGEIIAFNQDAADTPEDV
CHHHHCCEEEEECCCCCCEECCCCHHHHHHHHHHHHHHCCCCCCCEEEECCCCCCCCCCC
NGDPYEAWLFKIKLAAGANTDNLLSADAYKKLIGD
CCCCCEEEEEEEEEECCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: Lipoyl Cofactor. [C]

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NAD; L-glycine; THF [C]

Specific reaction: NAD + L-glycine + THF = Proton + NADH + NH3 + CO2 + 5,10-methylene-THF [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: NA