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The map label for this gene is dnaN [H]

Identifier: 78064660

GI number: 78064660

Start: 1822

End: 2928

Strand: Direct

Name: dnaN [H]

Synonym: Bcep18194_A3183

Alternate gene names: 78064660

Gene position: 1822-2928 (Clockwise)

Preceding gene: 78064659

Following gene: 78064661

Centisome position: 0.05

GC content: 63.69

Gene sequence:

>1107_bases
ATGCAACTGGTCAAGACCGAACGAGACACCCTCCTCAGGCCGCTGCAAACGGTCAGCGGCATCGTCGAACGCCGCCATAC
GTTGCCGATCCTCGCCAACCTGCTGATCACCAAGAACGGCCCGGACGTTTCGTTCCTGTCGACCGACCTGGAGCTGCAGA
TCACCACGCGCGCCGATTTCGGCGTCGGCGGCGACCAGGTCGCGACCACCGTCGCCGCGCGCAAGCTGCTCGACATCCTG
CGCGCGATGCCTGACGGCCAGGTCACGCTGTCGCTCGCCGACAAGCGCCTCACCGTTCAATCCGGCAAGAGCCGTTTCGC
ACTCCAGACGCTGGCGGCCGACGAGTTCCCGACCGTCGCGCAGGCGAAGGATTTCGGCGCAACGCTCACCGTCCCGCAGA
AGTCGTTCCGCCAGCTGCTCGGCATGGTGCACTTCGCGATGGCGCAGCAGGACATCCGCTACTACCTGAACGGCATGCTG
CTCGTCGTCGACGGCGACCAGCTGATGGCCGTGGCCACCGACGGCCACCGCCTCGCGTTCTCGTCGATGAAGCTCGAAGG
CGGCACGTTCGGCCGCCAGGAAGTCATCGTGCCGCGCAAGACGATTCTCGAGCTGCAACGCCTGCTCGAAGACATCGACG
ACACCGTCACCATCGACATCGCGCAAACCCAGGCCAAGTTCACGTTCGGCCAGGTCGAGCTCGTGTCGAAACTCGTCGAG
GGCAAGTTCCCCGACTTCCAGCGCGTGATCCCGAAGGCGCACAAGAACACGTTCGAGATCGGCCGTGAAGAGCTGCAGCG
TTCGCTGCAGCGCGCGGCGATCCTGACCTCGGACAAGTTCAAGGGCGTGCGCTGCATCATCGCGCCGGGCCAGCTGAAGA
TCATGTCGACCAACGCCGATCAGGAAGAGGCGCAGGAAGAACTGGAAATCGCCTACCAGGGCGATACCGTCGACATCGGC
TTCAACGTCACGTATCTGCTCGACGTACTCGCGAACCTGAAGGTCGACACCGTGCAGGTGAGCCTCGGCGACGCCAGCTC
GAGCGCGCTGATTACCGTGCCCGAGAACGAAGAGTTCAAGTATGTCGTGATGCCGATGCGCATCTGA

Upstream 100 bases:

>100_bases
ATACTGGTTTGACCGCCCCGGTGGTGGCGGGCCAAGAGCCCGGCCGGCGGGGCGCTGCGAGGCTGCTGCGCTGCAGGCCG
TTACTCAACGAAGGAACTCT

Downstream 100 bases:

>100_bases
CGCGCGCGACATCACGACACACACCAGGGGGCGGCAAGCCCCTTTGGCGTTTTTATGGCGAACCGACAACCCGTCCCTTT
GGTGCCTCACCGGTGCCTTG

Product: DNA polymerase III subunit beta

Products: NA

Alternate protein names: NA

Number of amino acids: Translated: 368; Mature: 368

Protein sequence:

>368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGATLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKLEGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENEEFKYVVMPMRI

Sequences:

>Translated_368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGATLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKLEGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENEEFKYVVMPMRI
>Mature_368_residues
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADFGVGGDQVATTVAARKLLDIL
RAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVAQAKDFGATLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGML
LVVDGDQLMAVATDGHRLAFSSMKLEGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
GKFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNADQEEAQEELEIAYQGDTVDIG
FNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENEEFKYVVMPMRI

Specific function: DNA polymerase III is a complex, multichain enzyme responsible for most of the replicative synthesis in bacteria. This DNA polymerase also exhibits 3' to 5' exonuclease activity. The beta chain is required for initiation of replication once it is clamped

COG id: COG0592

COG function: function code L; DNA polymerase sliding clamp subunit (PCNA homolog)

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: NA

Homologues:

Organism=Escherichia coli, GI1790136, Length=363, Percent_Identity=43.5261707988981, Blast_Score=302, Evalue=2e-83,

Paralogues:

None

Copy number: 5954 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001001
- InterPro:   IPR022635
- InterPro:   IPR022637
- InterPro:   IPR022634 [H]

Pfam domain/function: PF00712 DNA_pol3_beta; PF02767 DNA_pol3_beta_2; PF02768 DNA_pol3_beta_3 [H]

EC number: =2.7.7.7 [H]

Molecular weight: Translated: 40728; Mature: 40728

Theoretical pI: Translated: 5.14; Mature: 5.14

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.3 %Cys     (Translated Protein)
2.7 %Met     (Translated Protein)
3.0 %Cys+Met (Translated Protein)
0.3 %Cys     (Mature Protein)
2.7 %Met     (Mature Protein)
3.0 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCHH
QAKDFGATLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKLEGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
EEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHEEHHHHHHHHHHHC
GKFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNAD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
QEEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENEEFK
HHHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC
>Mature Secondary Structure
MQLVKTERDTLLRPLQTVSGIVERRHTLPILANLLITKNGPDVSFLSTDLELQITTRADF
CCCCCCCHHHHHHHHHHHHHHHHHHCCHHHHEEEEEECCCCCEEEEECCEEEEEEECCCC
GVGGDQVATTVAARKLLDILRAMPDGQVTLSLADKRLTVQSGKSRFALQTLAADEFPTVA
CCCHHHHHHHHHHHHHHHHHHHCCCCCEEEEEECCEEEECCCCCCEEHHHHCCCCCCCHH
QAKDFGATLTVPQKSFRQLLGMVHFAMAQQDIRYYLNGMLLVVDGDQLMAVATDGHRLAF
HHHHCCCEEECCHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEECCEEEEEECCCCEEEE
SSMKLEGGTFGRQEVIVPRKTILELQRLLEDIDDTVTIDIAQTQAKFTFGQVELVSKLVE
EEEEECCCCCCCCEEECCHHHHHHHHHHHHCCCCEEEEEEECCCHHEEHHHHHHHHHHHC
GKFPDFQRVIPKAHKNTFEIGREELQRSLQRAAILTSDKFKGVRCIIAPGQLKIMSTNAD
CCCCCHHHHHHHHCCCHHHHHHHHHHHHHHHHHHHCCCCCCCEEEEECCCEEEEEECCCC
QEEAQEELEIAYQGDTVDIGFNVTYLLDVLANLKVDTVQVSLGDASSSALITVPENEEFK
HHHHHHHHEEEECCCEEEECCHHHHHHHHHHCCEEEEEEEEECCCCCCEEEECCCCCCEE
YVVMPMRI
EEEEEECC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12534463 [H]