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The map label for this gene is kgtP [H]

Identifier: 78049964

GI number: 78049964

Start: 5083463

End: 5084824

Strand: Reverse

Name: kgtP [H]

Synonym: XCV4408

Alternate gene names: 78049964

Gene position: 5084824-5083463 (Counterclockwise)

Preceding gene: 78049965

Following gene: 78049958

Centisome position: 98.19

GC content: 62.56

Gene sequence:

>1362_bases
ATGAGCGCACCCCCATCGTCTCCGATCGCTGCCGGTTCTGGCACAGCTGCCGGCAGCCTTCGCCGCTCGGTGTCCAACAC
GCTCAAGGGCTCGGCCGGCAACCTCGTCGAGTGGTACGACGTCTACGTCTATTCGGTGTTCGCCACCTACTTCGAGTCGC
AGTTCTTTTCCAAGGAAGACAAGAACGCCACCATGTTCGTGTGGGCGATCTTTGCGATGACGTTCCTGATGCGCCCGATC
GGCGCGTGGTATTTCGGCCGCTTCGCCGACCGTTATGGCCGCCGCCTGGCGTTGACCATTTCGGTATCGGTGATGGCGCT
GTGCTCGTTCGTGATTGCGATCACGCCCACGGTGGCCACGATCGGCATCGCTGCGCCGATCATCTTGCTGGTGGCGCGCC
TGCTGCAGGGCTTTGCGACCGGCGGCGAATACGGCACCAGCGCCACGTACATGTCCGAAGCGGCGATTCCCGGCCGGCGC
GGGTTTTTGTCGTCGTTCCATTACGTGACGCTGGTGGGCGGGCACGTACTGGCACAGACCACCTTGCTGATCATGTTGCA
TTTCTTCGATGCCTCGCAGGTCTCCGAATGGGGCTGGCGCGTGGCGTTCGGGCTGGGCGGCATCGGCGCGTTGGTGGTGT
TCTGGCTGCGCCGCACGATGGATGAGTCGCTGAGCTCCGAATCCATCGCCGATTCGCGTACCGGCAAGGCCAAGGCCTCC
GGCTCGATGCGCGAATTGTTCGTCAATCAGTGGCGTCCGCTGCTGTTGTGCTTTTTGATCACCGCCGGCGGCACGATCGC
GTTCTACACCTATTCGGTCACCGGGCCGAAGATGATCCAGACCGCGTTCGCCGGTGGCGACGTGATGGCCGGCACCATCA
TCAATCTGGTGGCGCTGACCGTGCTGATGCTGATGCAGCCGGTGGGCGGCTGGTTGTCGGACATCGTCGGGCGCAAGACC
TTGCTGGTGTTCTTCGGCATCGGCGGCGTGCTCTACACCTGGTTCCTGGTGATGGAACTGCCCAAGCAGACCGACTGGTT
GACCGCGTTCGCAATCCTCACCGTGGGCTTTGTGATCCTGACCGGCTACACCTCGATCAATGCCGTGGTGAAGGCGGAAT
TGTTCCCCACCCACGTGCGCGCGCTGGGTGTCGGCCTTGGCTACGCGCTGGCCAATTCGGCCTTCGGCGGAACCGCGCCG
TTGCTGTATCAGGCCTCGCTGAAGACCAACCACGTGACCGAGTTCGTGATCTATGCGACGGCGGTGATTGCGGTGTCGTT
GGTGGTCTACATGTTCTTCCTGACCAACAAGGGCAGCAACTGGCTGGACGACGAAGCGGCGATGCATCAGCGCAAGCGGT
GA

Upstream 100 bases:

>100_bases
GTGTGCGAGTTGCCGCCATCTATAAGCCAAAATGCTTACATGCGTCACACAAGCGCGCACCTATACTCCGGTGCTGTCCC
ACCGAAGAGGTTCTGACCGC

Downstream 100 bases:

>100_bases
TGCGCGCGCGCGGGCAGAGCCCTTCCCTTCTCCCTGCGGGACATTGTCCCCCTTCATGGGGGAGAAGGTGCCCGAAGGGC
GGATGAGGGGCGCCTCAGGT

Product: metabolite:H+ symporter family protein

Products: Proton [Cytoplasm]; 2-oxoglutarate [Cytoplasm] [C]

Alternate protein names: NA

Number of amino acids: Translated: 453; Mature: 452

Protein sequence:

>453_residues
MSAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLIMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNHVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDEAAMHQRKR

Sequences:

>Translated_453_residues
MSAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPI
GAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRR
GFLSSFHYVTLVGGHVLAQTTLLIMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKT
LLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAP
LLYQASLKTNHVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDEAAMHQRKR
>Mature_452_residues
SAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKEDKNATMFVWAIFAMTFLMRPIG
AWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVATIGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRRG
FLSSFHYVTLVGGHVLAQTTLLIMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKASG
SMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALTVLMLMQPVGGWLSDIVGRKTL
LVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVILTGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAPL
LYQASLKTNHVTEFVIYATAVIAVSLVVYMFFLTNKGSNWLDDEAAMHQRKR

Specific function: Uptake of alpha-ketoglutarate across the boundary membrane with the concomitant import of a cation (symport system) [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. Sugar transporter (TC 2.A.1.1) family [H]

Homologues:

Organism=Escherichia coli, GI1788942, Length=436, Percent_Identity=41.0550458715596, Blast_Score=289, Evalue=3e-79,
Organism=Escherichia coli, GI1790550, Length=375, Percent_Identity=28, Blast_Score=160, Evalue=2e-40,
Organism=Escherichia coli, GI1788292, Length=431, Percent_Identity=29.0023201856148, Blast_Score=137, Evalue=1e-33,
Organism=Escherichia coli, GI1789941, Length=387, Percent_Identity=30.4909560723514, Blast_Score=117, Evalue=1e-27,
Organism=Escherichia coli, GI87082231, Length=221, Percent_Identity=27.1493212669683, Blast_Score=70, Evalue=2e-13,
Organism=Escherichia coli, GI87082404, Length=354, Percent_Identity=25.4237288135593, Blast_Score=67, Evalue=3e-12,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR004736
- InterPro:   IPR020846
- InterPro:   IPR016196
- InterPro:   IPR005828
- InterPro:   IPR005829 [H]

Pfam domain/function: PF00083 Sugar_tr [H]

EC number: NA

Molecular weight: Translated: 49112; Mature: 48981

Theoretical pI: Translated: 9.64; Mature: 9.64

Prosite motif: PS50850 MFS ; PS00216 SUGAR_TRANSPORT_1 ; PS00217 SUGAR_TRANSPORT_2

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.4 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.0 %Cys+Met (Translated Protein)
0.4 %Cys     (Mature Protein)
3.3 %Met     (Mature Protein)
3.8 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MSAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKED
CCCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KNATMFVWAIFAMTFLMRPIGAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRRGFLSSFHYVTLVGGHVLAQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHCCHHHHHCHHHHHHH
TLLIMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
HHHHHHHHHCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALT
CHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
VLMLMQPVGGWLSDIVGRKTLLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVIL
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAPLLYQASLKTNHVTEFVIYAT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
AVIAVSLVVYMFFLTNKGSNWLDDEAAMHQRKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC
>Mature Secondary Structure 
SAPPSSPIAAGSGTAAGSLRRSVSNTLKGSAGNLVEWYDVYVYSVFATYFESQFFSKED
CCCCCCCCCCCCCCHHHHHHHHHHHHHCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCC
KNATMFVWAIFAMTFLMRPIGAWYFGRFADRYGRRLALTISVSVMALCSFVIAITPTVAT
CCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCEEEEEEHHHHHHHHHHHHHHHHHHHH
IGIAAPIILLVARLLQGFATGGEYGTSATYMSEAAIPGRRGFLSSFHYVTLVGGHVLAQT
HHHHHHHHHHHHHHHHHHCCCCCCCCCHHHHHHHCCCCCHHHHHHCCHHHHHCHHHHHHH
TLLIMLHFFDASQVSEWGWRVAFGLGGIGALVVFWLRRTMDESLSSESIADSRTGKAKAS
HHHHHHHHHCHHHHHHCCCEEEECCCCHHHHHHHHHHHHHHHHCCHHHHHCCCCCCCCCC
GSMRELFVNQWRPLLLCFLITAGGTIAFYTYSVTGPKMIQTAFAGGDVMAGTIINLVALT
CHHHHHHHHCCHHHHHHHHHHCCCEEEEEEEECCCHHHHHHHHCCCCHHHHHHHHHHHHH
VLMLMQPVGGWLSDIVGRKTLLVFFGIGGVLYTWFLVMELPKQTDWLTAFAILTVGFVIL
HHHHHHHCCHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHHHH
TGYTSINAVVKAELFPTHVRALGVGLGYALANSAFGGTAPLLYQASLKTNHVTEFVIYAT
HCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCCCCEEEECCCCCHHHHHHHHHH
AVIAVSLVVYMFFLTNKGSNWLDDEAAMHQRKR
HHHHHHHHHHHHHHHCCCCCCCCHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; 2-oxoglutarate [Periplasm] [C]

Specific reaction: Proton [Periplasm] + 2-oxoglutarate [Periplasm] = Proton [Cytoplasm] + 2-oxoglutarate [Cytoplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 2118499; 9205837; 9278503; 1709493; 2190187; 2053984; 8419306 [H]