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The map label for this gene is tetA [H]

Identifier: 78049950

GI number: 78049950

Start: 5064884

End: 5066233

Strand: Reverse

Name: tetA [H]

Synonym: XCV4394

Alternate gene names: 78049950

Gene position: 5066233-5064884 (Counterclockwise)

Preceding gene: 78049951

Following gene: 78049949

Centisome position: 97.83

GC content: 68.3

Gene sequence:

>1350_bases
TTGGGCGCATCAGACGACAGCCCACCCCAACCCCACCATTCAATCCACCGCTACACTCGCCGCTTCGCCCAACTGCGCCG
CGCGATGTCCCTCTCTCCCGATTCCGCTCCCGTCCCCGGGCGCCGCCGCGCCGCGCTGATCTTCATCTTCATCACCGTCC
TGATCGACGTGCTGTCCTTCGGGGTGATCATTCCGGTACTTCCGGATCTGGTGCGCCACTTCACCGGCGGCGACTACGTG
GTGGCCGCCGGCTGGATCGGCTGGTTCGGTTTCCTGTTCGCTGCCATCCAGTTCGTCTGCTCGCCATTGCAGGGCGCGCT
CTCCGATCGCTTCGGTCGCCGCCCGGTGATCCTGCTCTCGTGCCTGGGCCTGGGCCTGGACTTCATCCTGATGGCCATTG
CCCACAGCCTGCCGATGCTGCTGCTGGCGCGGGTCATCTCCGGCGTGTGCTCGGCCAGCTTCTCCACCGCCAATGCCTAC
ATCGCCGATGTCACCCCACCGGACAAACGCGCCGGCGCATTCGGCATGCTCGGTGCCGCCTTCGGCATCGGCTTTGTCGC
CGGCCCGTTGATCGGGGGTTGGCTGGGCAGCATCGGGTTGCGCTGGCCGTTCTGGTTTGCCGCCGGGCTGGCCCTGTTGA
ACGTGCTGTATGGCTGGTTCGTGCTGCCCGAATCGCTGCCCGCCGAACGCCGTACCGCGCGGCTGGACTGGTCGCACGCC
AATCCGCTGGGCGCGCTCAAGCTGCTGCGCCGCTACCCGCAGGTGTTCGGCCTGGCGTCGGTGGTGTTCCTGGCCAACCT
GGCGCATTACGTGTATCCCAGCATTTTCGTGCTGTTCGCCGGCTACCAATACCATTGGGGCCCGCGCGAAGTGAGCTGGG
TGCTGGCCGGGGTCGGTGTCTGCAGCATCATCGTCAATGCGTTGCTGGTCGGCCGCCTGGTGCGCCGGCTCGGCGAACGC
CGCGCGTTGCTGCTGGGGCTGGGCTGCGGCGTGATCGGCTTCATCATCTATGGGCTGGCCGACAGCGGCACTGCCTTCCT
GATCGGTGTGCCGATCAGTGCGTTGTGGGCCATCGCCGCACCGTCCGCGCAGGCCCTGATCACCCGTGAGGTCGGCGCCG
ACGCGCAAGGTCGCGTGCAGGGCGCGCTCACCGGCCTGGTCAGCCTGGCCGGCATCGCCGGTCCATTGTTGTTCGCCAAT
GTCTTCGCCTGGTTCATCGGCAGCGGTGCACCGCTGCATCTGCCGGGCGCGCCGTGGTTGCTGGCCGCTGTACTGCTGGC
CGCAGGCTGGGCCATGGCCTGGAAACGGGCCGCGCGCGGCGCCGGCACCAGCCCCGCGGCGCAGCCGTGA

Upstream 100 bases:

>100_bases
GCGCGAAGCCTGGTGGAGGCTGAGCGCACATGTCGCTTCGCCCCGTACCCTCACCCCAACCCCTCTCCCGCAGGAGAGGG
GCTTTCAGTAGAGGGGGACC

Downstream 100 bases:

>100_bases
AGCCAACCTATTCAGCTTGCGCTCGCGTCTGCTTTTTCTGATTTGCGCAGGCACTGCTAGGCTGGTGTCCTTCGTGCCGC
GGCACCGCTTTGAGCCCCTA

Product: drug:H+ antiporter-1 family protein

Products: Proton [Cytoplasm]; drug [Periplasm] [C]

Alternate protein names: TetA(A) [H]

Number of amino acids: Translated: 449; Mature: 448

Protein sequence:

>449_residues
MGASDDSPPQPHHSIHRYTRRFAQLRRAMSLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYV
VAAGWIGWFGFLFAAIQFVCSPLQGALSDRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAY
IADVTPPDKRAGAFGMLGAAFGIGFVAGPLIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAERRTARLDWSHA
NPLGALKLLRRYPQVFGLASVVFLANLAHYVYPSIFVLFAGYQYHWGPREVSWVLAGVGVCSIIVNALLVGRLVRRLGER
RALLLGLGCGVIGFIIYGLADSGTAFLIGVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIAGPLLFAN
VFAWFIGSGAPLHLPGAPWLLAAVLLAAGWAMAWKRAARGAGTSPAAQP

Sequences:

>Translated_449_residues
MGASDDSPPQPHHSIHRYTRRFAQLRRAMSLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYV
VAAGWIGWFGFLFAAIQFVCSPLQGALSDRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAY
IADVTPPDKRAGAFGMLGAAFGIGFVAGPLIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAERRTARLDWSHA
NPLGALKLLRRYPQVFGLASVVFLANLAHYVYPSIFVLFAGYQYHWGPREVSWVLAGVGVCSIIVNALLVGRLVRRLGER
RALLLGLGCGVIGFIIYGLADSGTAFLIGVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIAGPLLFAN
VFAWFIGSGAPLHLPGAPWLLAAVLLAAGWAMAWKRAARGAGTSPAAQP
>Mature_448_residues
GASDDSPPQPHHSIHRYTRRFAQLRRAMSLSPDSAPVPGRRRAALIFIFITVLIDVLSFGVIIPVLPDLVRHFTGGDYVV
AAGWIGWFGFLFAAIQFVCSPLQGALSDRFGRRPVILLSCLGLGLDFILMAIAHSLPMLLLARVISGVCSASFSTANAYI
ADVTPPDKRAGAFGMLGAAFGIGFVAGPLIGGWLGSIGLRWPFWFAAGLALLNVLYGWFVLPESLPAERRTARLDWSHAN
PLGALKLLRRYPQVFGLASVVFLANLAHYVYPSIFVLFAGYQYHWGPREVSWVLAGVGVCSIIVNALLVGRLVRRLGERR
ALLLGLGCGVIGFIIYGLADSGTAFLIGVPISALWAIAAPSAQALITREVGADAQGRVQGALTGLVSLAGIAGPLLFANV
FAWFIGSGAPLHLPGAPWLLAAVLLAAGWAMAWKRAARGAGTSPAAQP

Specific function: Resistance to tetracycline by an active tetracycline efflux. This is an energy-dependent process that decreases the accumulation of the antibiotic in whole cells. This protein functions as a metal-tetracycline/H(+) antiporter [H]

COG id: NA

COG function: NA

Gene ontology:

Cell location: Cell inner membrane; Multi-pass membrane protein [H]

Metaboloic importance: Unknown [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the major facilitator superfamily. TCR/tet family [H]

Homologues:

Organism=Homo sapiens, GI39753965, Length=316, Percent_Identity=31.9620253164557, Blast_Score=146, Evalue=5e-35,
Organism=Homo sapiens, GI94721311, Length=439, Percent_Identity=27.7904328018223, Blast_Score=137, Evalue=2e-32,
Organism=Homo sapiens, GI225703104, Length=167, Percent_Identity=35.9281437125749, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI225703102, Length=167, Percent_Identity=35.9281437125749, Blast_Score=99, Evalue=9e-21,
Organism=Homo sapiens, GI217416366, Length=481, Percent_Identity=25.7796257796258, Blast_Score=68, Evalue=2e-11,
Organism=Escherichia coli, GI1787291, Length=388, Percent_Identity=25, Blast_Score=69, Evalue=8e-13,
Organism=Caenorhabditis elegans, GI71996331, Length=378, Percent_Identity=27.7777777777778, Blast_Score=133, Evalue=2e-31,
Organism=Caenorhabditis elegans, GI17551570, Length=228, Percent_Identity=23.6842105263158, Blast_Score=89, Evalue=4e-18,
Organism=Drosophila melanogaster, GI21357569, Length=361, Percent_Identity=27.9778393351801, Blast_Score=123, Evalue=2e-28,
Organism=Drosophila melanogaster, GI24656463, Length=410, Percent_Identity=26.5853658536585, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI24656468, Length=410, Percent_Identity=26.5853658536585, Blast_Score=121, Evalue=8e-28,
Organism=Drosophila melanogaster, GI161080926, Length=410, Percent_Identity=26.5853658536585, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI161080924, Length=410, Percent_Identity=26.5853658536585, Blast_Score=121, Evalue=1e-27,
Organism=Drosophila melanogaster, GI17738085, Length=457, Percent_Identity=25.164113785558, Blast_Score=118, Evalue=1e-26,
Organism=Drosophila melanogaster, GI221513297, Length=360, Percent_Identity=27.5, Blast_Score=113, Evalue=3e-25,
Organism=Drosophila melanogaster, GI24667557, Length=163, Percent_Identity=28.8343558282209, Blast_Score=77, Evalue=2e-14,
Organism=Drosophila melanogaster, GI24667561, Length=166, Percent_Identity=27.710843373494, Blast_Score=75, Evalue=1e-13,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR020846
- InterPro:   IPR011701
- InterPro:   IPR016196
- InterPro:   IPR004734
- InterPro:   IPR005829
- InterPro:   IPR001958 [H]

Pfam domain/function: PF07690 MFS_1 [H]

EC number: NA

Molecular weight: Translated: 47679; Mature: 47548

Theoretical pI: Translated: 10.59; Mature: 10.59

Prosite motif: PS00141 ASP_PROTEASE ; PS50850 MFS

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.1 %Cys     (Translated Protein)
1.3 %Met     (Translated Protein)
2.4 %Cys+Met (Translated Protein)
1.1 %Cys     (Mature Protein)
1.1 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure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HHHHHHHHHHHHHHHHHHCCCCCCCCCCC
>Mature Secondary Structure 
GASDDSPPQPHHSIHRYTRRFAQLRRAMSLSPDSAPVPGRRRAALIFIFITVLIDVLSF
CCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHH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HHHHHHHHHHHHHHHHHHCCCCCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Alpha

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: Proton [Periplasm]; drug [Cytoplasm] [C]

Specific reaction: Proton [Periplasm] + drug [Cytoplasm] = Proton [Cytoplasm] + drug [Periplasm] [C]

General reaction: NA

Inhibitor: NA

Structure determination priority: 6.0

TargetDB status: NA

Availability: NA

References: 1312499; 6310527 [H]