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The map label for this gene is tyrS [H]

Identifier: 78049566

GI number: 78049566

Start: 4606827

End: 4608038

Strand: Reverse

Name: tyrS [H]

Synonym: XCV4010

Alternate gene names: 78049566

Gene position: 4608038-4606827 (Counterclockwise)

Preceding gene: 78049569

Following gene: 78049565

Centisome position: 88.98

GC content: 63.2

Gene sequence:

>1212_bases
TTGGCCACTATCGAAGAATCCCTCGCACTCATCGGCCGTGGTGCCGAAGAGATCCTCAAGCTCGATCAGCTGCAGGCGCG
GCTCGCCACCGGTAAGCCGCTGCGGGTGAAGGCCGGCTTCGATCCCACCGCGCCGGATCTGCATCTGGGCCATACCGTGC
TGCTCAACAAGATGCGGCAGTTCCAACAGCTTGGCCATCAGGTGATCTTCCTGATTGGCGACTTCACCGGAATGATCGGC
GACCCGAGCGGCAAGAACGCCACCCGCAAGCCGCTCAGCCGCGAGGATGTGCTGGCCAACGCACGGACCTACGAAGAGCA
GGTCTTCAAGATCCTGGACCGCGAACGCACCGAGGTGCGTTTCAACTCCGAATGGTTCGGCCAGATGAGCGCGGCCGACA
TGATCAAGCTCTCTGCCCAGCACACCGTCGCGCGCATGCTCGAACGCGACGATTTCGCCAAGCGCTTCGGCAGCCAGCAG
CCGATCGCCATCCACGAATTCCTCTACCCGTTGGTGCAAGGCTACGATTCGGTCGCCCTGAAGGCGGACGTCGAGCTGGG
CGGCACCGATCAGAAGTTCAACCTGTTGATGGGGCGCGGCTTGCAGGAGCATTACGGTCAGGCGCCGCAGATCGTGCTGA
CCATGCCGCTGCTGGAAGGCCTGGACGGCGTAGCCAAGATGTCCAAGTCGTTGGGCAATTACATCGGCATCAACGAGCCG
GCCATCGACATCGTCACCAAGACGATGAAGATCGGCGACGAGCTGACCTGGCGCTGGATCGACTTGCTGTCCTTCGATAT
CAGCCTGGCCGAAGCGGCGCGCCTGAAAGAGCAGGTCGCTGCGGGAGAGCTGCATCCGCGCGAGGTCAAATTGCGCCTGG
CACGCGAGCTGGCCACCCGGTTTCACGATGCCGCAACCGCAGAGCAGGCCATTGCCGGCTGGCACGCGGTGGTAACGGGG
CAGGGCGACACCAGCCTGCTACCGCTGCAGGAAGTGGTGGTTCCCGATGAGGGCCTGCGCCTTGCCGGGCTCCTGACCGC
CGCCGGCCTGACGCCCAGCAACTCCGAAGCCACCCGCAAGCTCAAGGAACGCGCGGTCAGGATCGACGGCGAAGTAGTGG
AGGATCCCAGCCGCGTCTTTACGCACGGCTTCGAAGGGGTCATCCAGGTAGGCAAGCGTAATTTTGCCCGCGTATTGCTC
GTCACTGCCTAA

Upstream 100 bases:

>100_bases
TCAAACCATTGAGCCTGTTCATTATTTTCACTTGGCGCGGAATTAACCCGCGTTTAACATCATTCACGTTTTTGACGGCA
AGCGCCGCTGGAGTCTGTGG

Downstream 100 bases:

>100_bases
AGCGCACCAAGGTTGCCGCAGGGCGGCAATCTCGGTGATCTCACGCGTCTTACACGTTGGATAGAAGTGACAGTCCCCAG
GCCCTCAAAGCTTTTCTGCT

Product: tyrosyl-tRNA synthetase

Products: NA

Alternate protein names: Tyrosine--tRNA ligase; TyrRS [H]

Number of amino acids: Translated: 403; Mature: 402

Protein sequence:

>403_residues
MATIEESLALIGRGAEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDELTWRWIDLLSFDISLAEAARLKEQVAAGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTSLLPLQEVVVPDEGLRLAGLLTAAGLTPSNSEATRKLKERAVRIDGEVVEDPSRVFTHGFEGVIQVGKRNFARVLL
VTA

Sequences:

>Translated_403_residues
MATIEESLALIGRGAEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIG
DPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQ
PIAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
AIDIVTKTMKIGDELTWRWIDLLSFDISLAEAARLKEQVAAGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTG
QGDTSLLPLQEVVVPDEGLRLAGLLTAAGLTPSNSEATRKLKERAVRIDGEVVEDPSRVFTHGFEGVIQVGKRNFARVLL
VTA
>Mature_402_residues
ATIEESLALIGRGAEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQFQQLGHQVIFLIGDFTGMIGD
PSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVRFNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQP
IAIHEFLYPLVQGYDSVALKADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEPA
IDIVTKTMKIGDELTWRWIDLLSFDISLAEAARLKEQVAAGELHPREVKLRLARELATRFHDAATAEQAIAGWHAVVTGQ
GDTSLLPLQEVVVPDEGLRLAGLLTAAGLTPSNSEATRKLKERAVRIDGEVVEDPSRVFTHGFEGVIQVGKRNFARVLLV
TA

Specific function: Catalyzes the attachment of tyrosine to tRNA(Tyr) in a two-step reaction:tyrosine is first activated by ATP to form Tyr- AMP and then transferred to the acceptor end of tRNA(Tyr) [H]

COG id: COG0162

COG function: function code J; Tyrosyl-tRNA synthetase

Gene ontology:

Cell location: Cytoplasm [H]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Contains 1 S4 RNA-binding domain [H]

Homologues:

Organism=Homo sapiens, GI94681057, Length=425, Percent_Identity=27.0588235294118, Blast_Score=107, Evalue=2e-23,
Organism=Escherichia coli, GI1787925, Length=402, Percent_Identity=28.8557213930348, Blast_Score=136, Evalue=2e-33,
Organism=Caenorhabditis elegans, GI25145802, Length=210, Percent_Identity=32.8571428571429, Blast_Score=102, Evalue=3e-22,
Organism=Saccharomyces cerevisiae, GI6325160, Length=246, Percent_Identity=28.0487804878049, Blast_Score=91, Evalue=3e-19,
Organism=Drosophila melanogaster, GI20130383, Length=402, Percent_Identity=24.8756218905473, Blast_Score=117, Evalue=1e-26,

Paralogues:

None

Copy number: 892 Molecules/Cell In: Growth Phase, Glucose-minimal MOPS Media. 3,000 Molecules/Cell In: Glucose minimal media [C]

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR001412
- InterPro:   IPR002305
- InterPro:   IPR014729
- InterPro:   IPR002942
- InterPro:   IPR002307 [H]

Pfam domain/function: PF00579 tRNA-synt_1b [H]

EC number: =6.1.1.1 [H]

Molecular weight: Translated: 44476; Mature: 44345

Theoretical pI: Translated: 6.37; Mature: 6.37

Prosite motif: PS50889 S4 ; PS00178 AA_TRNA_LIGASE_I

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

0.0 %Cys     (Translated Protein)
2.5 %Met     (Translated Protein)
2.5 %Cys+Met (Translated Protein)
0.0 %Cys     (Mature Protein)
2.2 %Met     (Mature Protein)
2.2 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MATIEESLALIGRGAEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDELTWRWIDLLSFDISLAEAARLKEQVAAGELHPREVKLRLARELATR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTSLLPLQEVVVPDEGLRLAGLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
LKERAVRIDGEVVEDPSRVFTHGFEGVIQVGKRNFARVLLVTA
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEC
>Mature Secondary Structure 
ATIEESLALIGRGAEEILKLDQLQARLATGKPLRVKAGFDPTAPDLHLGHTVLLNKMRQ
CCHHHHHHHHCCCHHHHHHHHHHHHHHHCCCCEEEECCCCCCCCCCCCHHHHHHHHHHH
FQQLGHQVIFLIGDFTGMIGDPSGKNATRKPLSREDVLANARTYEEQVFKILDRERTEVR
HHHHCHHEEEEEECCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHCHHHHEE
FNSEWFGQMSAADMIKLSAQHTVARMLERDDFAKRFGSQQPIAIHEFLYPLVQGYDSVAL
ECHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHHHCCCCCEEE
KADVELGGTDQKFNLLMGRGLQEHYGQAPQIVLTMPLLEGLDGVAKMSKSLGNYIGINEP
EEEEEECCCCCHHHHHHCCCCHHHCCCCCEEEEEEHHHHCCHHHHHHHHHHCCCCCCCCC
AIDIVTKTMKIGDELTWRWIDLLSFDISLAEAARLKEQVAAGELHPREVKLRLARELATR
HHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCCHHHHHHHHHHHHHHH
FHDAATAEQAIAGWHAVVTGQGDTSLLPLQEVVVPDEGLRLAGLLTAAGLTPSNSEATRK
HHHHHHHHHHHHCCEEEEECCCCCCCCCHHHHHCCCCCCHHHHHHHHCCCCCCCHHHHHH
LKERAVRIDGEVVEDPSRVFTHGFEGVIQVGKRNFARVLLVTA
HHHHHHHCCCHHHCCHHHHHHHHHHHHHHHCCCCEEEEEEEEC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 9.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]