Click here to switch to the map view.

The map label for this gene is vanX [H]

Identifier: 78049533

GI number: 78049533

Start: 4566464

End: 4567210

Strand: Reverse

Name: vanX [H]

Synonym: XCV3977

Alternate gene names: 78049533

Gene position: 4567210-4566464 (Counterclockwise)

Preceding gene: 78049534

Following gene: 78049532

Centisome position: 88.2

GC content: 64.66

Gene sequence:

>747_bases
ATGACCGACAACCTGCTGTTCCGGAATGGGCGCTGCTGGCGTCAGTGCTTTGTTCAAGGCGCTTTGCTGATGAGCGCCGT
GGCGCCATTTGCGCTGGTCGCCGGCCAGGTGCATATGTCCTCGGCAAGGACCGCGGCAGAGGCCGGCCTTGTGGACGTGC
ACGCCCTCGCGCCGGATATCGCATTGGACATGCGGTATGCGAGCAGCAACAACTTCACTGGGCGGATGGTACCGGGCTAT
GACGCGCCGCGGTGTTACCTGTTGCGGCCGGCTGCCGACGCATTGGCGCGGGTAGCGCAGACGCTCAAGGCCGAGGGCTA
TCGGCTACAGGTGTTCGATTGCTATCGGCCGGTGCGGTCGGTGAAGGCATTCGTCACCTGGGCTGCGGATCTGCAGGATC
AATCGACCAAGGCGCAGTACTACCCGCGGGTGGACAAGCGCGCACTGCTGGGCGATTACATTGCGCCCACCTCCGGGCAC
AGCCGTGGCGCGACGCTGGACCTGGGCTTGCTGGAGTGCCTGCCGGGCGGGTGCCGTGCAGTGGACATGGGCACAGGATT
CGACTTCTTCGATGCGCGGGCGCACACCAACACGCCCGACATCAGCGCAGCGCAACGCGCGCACCGCCAGCGCCTGTTGC
AGGCGATGGCGGCCGAAGGATTTTCCAACTATCCGATGGAATGGTGGCATTTCACGTTGCGTCCCGAACCCACGCCCGAG
ACGGCCTACGATGTTCCGGTAGACTGA

Upstream 100 bases:

>100_bases
GACCACTAGGCCCACAGGGATCGCGCCACGCTGTAGCCGGTGGCGAAAGACGCGCGGCTTGATCTACCGCGCAAATGCGG
CCTCTTGCAAGGGAACCGAA

Downstream 100 bases:

>100_bases
CAGCGACCAGGCAACACGCTCTTGGTCGCTGATCGATCTGGTCGTGCTCGCGATCGGTTGGCCGCTCGCGAGAGCCGTGG
AGGCAGGCATCGTGTCGACG

Product: D-alanyl-D-alanine dipeptidase precursor

Products: NA

Alternate protein names: D-Ala-D-Ala dipeptidase; Vancomycin B-type resistance protein vanX [H]

Number of amino acids: Translated: 248; Mature: 247

Protein sequence:

>248_residues
MTDNLLFRNGRCWRQCFVQGALLMSAVAPFALVAGQVHMSSARTAAEAGLVDVHALAPDIALDMRYASSNNFTGRMVPGY
DAPRCYLLRPAADALARVAQTLKAEGYRLQVFDCYRPVRSVKAFVTWAADLQDQSTKAQYYPRVDKRALLGDYIAPTSGH
SRGATLDLGLLECLPGGCRAVDMGTGFDFFDARAHTNTPDISAAQRAHRQRLLQAMAAEGFSNYPMEWWHFTLRPEPTPE
TAYDVPVD

Sequences:

>Translated_248_residues
MTDNLLFRNGRCWRQCFVQGALLMSAVAPFALVAGQVHMSSARTAAEAGLVDVHALAPDIALDMRYASSNNFTGRMVPGY
DAPRCYLLRPAADALARVAQTLKAEGYRLQVFDCYRPVRSVKAFVTWAADLQDQSTKAQYYPRVDKRALLGDYIAPTSGH
SRGATLDLGLLECLPGGCRAVDMGTGFDFFDARAHTNTPDISAAQRAHRQRLLQAMAAEGFSNYPMEWWHFTLRPEPTPE
TAYDVPVD
>Mature_247_residues
TDNLLFRNGRCWRQCFVQGALLMSAVAPFALVAGQVHMSSARTAAEAGLVDVHALAPDIALDMRYASSNNFTGRMVPGYD
APRCYLLRPAADALARVAQTLKAEGYRLQVFDCYRPVRSVKAFVTWAADLQDQSTKAQYYPRVDKRALLGDYIAPTSGHS
RGATLDLGLLECLPGGCRAVDMGTGFDFFDARAHTNTPDISAAQRAHRQRLLQAMAAEGFSNYPMEWWHFTLRPEPTPET
AYDVPVD

Specific function: Hydrolyzes D-Ala-D-Ala. Has a 250-fold differential in catalytic efficiency for hydrolysis of D-Ala-D-Ala versus D-Ala-D- lactate. Thus D-Ala-D-lactate remains intact for subsequent incorporation into peptidoglycan precursors that terminate in the depsipe

COG id: COG2173

COG function: function code M; D-alanyl-D-alanine dipeptidase

Gene ontology:

Cell location: Secreted (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the peptidase M15D family [H]

Homologues:

Organism=Escherichia coli, GI1787763, Length=184, Percent_Identity=32.0652173913043, Blast_Score=89, Evalue=2e-19,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR009045
- InterPro:   IPR000755 [H]

Pfam domain/function: PF01427 Peptidase_M15 [H]

EC number: =3.4.13.22 [H]

Molecular weight: Translated: 27381; Mature: 27250

Theoretical pI: Translated: 7.46; Mature: 7.46

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.4 %Cys     (Translated Protein)
3.2 %Met     (Translated Protein)
5.6 %Cys+Met (Translated Protein)
2.4 %Cys     (Mature Protein)
2.8 %Met     (Mature Protein)
5.3 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MTDNLLFRNGRCWRQCFVQGALLMSAVAPFALVAGQVHMSSARTAAEAGLVDVHALAPDI
CCCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCH
ALDMRYASSNNFTGRMVPGYDAPRCYLLRPAADALARVAQTLKAEGYRLQVFDCYRPVRS
HEEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VKAFVTWAADLQDQSTKAQYYPRVDKRALLGDYIAPTSGHSRGATLDLGLLECLPGGCRA
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHCCCCCEE
VDMGTGFDFFDARAHTNTPDISAAQRAHRQRLLQAMAAEGFSNYPMEWWHFTLRPEPTPE
EECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCC
TAYDVPVD
CCCCCCCC
>Mature Secondary Structure 
TDNLLFRNGRCWRQCFVQGALLMSAVAPFALVAGQVHMSSARTAAEAGLVDVHALAPDI
CCCEEEECCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEHHHHCCCH
ALDMRYASSNNFTGRMVPGYDAPRCYLLRPAADALARVAQTLKAEGYRLQVFDCYRPVRS
HEEEEECCCCCCCEEECCCCCCCEEEEECCHHHHHHHHHHHHHCCCCEEEEHHHHHHHHH
VKAFVTWAADLQDQSTKAQYYPRVDKRALLGDYIAPTSGHSRGATLDLGLLECLPGGCRA
HHHHHHHHHHHCCCCCCHHHCCCCHHHHHHHHHCCCCCCCCCCCEEECHHHHHCCCCCEE
VDMGTGFDFFDARAHTNTPDISAAQRAHRQRLLQAMAAEGFSNYPMEWWHFTLRPEPTPE
EECCCCCCHHCCCCCCCCCCHHHHHHHHHHHHHHHHHHCCCCCCCHHHEEEEECCCCCCC
TAYDVPVD
CCCCCCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 8380148; 7854121; 7873524 [H]