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The map label for this gene is queF [H]

Identifier: 78049519

GI number: 78049519

Start: 4549638

End: 4550453

Strand: Reverse

Name: queF [H]

Synonym: XCV3963

Alternate gene names: 78049519

Gene position: 4550453-4549638 (Counterclockwise)

Preceding gene: 78049520

Following gene: 78049518

Centisome position: 87.87

GC content: 64.09

Gene sequence:

>816_bases
ATGAATACCCCAGAAGATTCCACCCTCGGTCGTGAGGTCGCCTATCCGAGCGGCTACGACCCGTCGTTGCTGTTTCCGAT
TCCGCGTGCGGCCGGGCGCCAGGCCATCGGGCTGACCGGTGAGCTGCCGTTTATCGGCCGCGATCGCTGGCACGCCTACG
AACTCAGCTGGCTCGATGCACAAGGCAAGCCCTGCGTGGCCACCGCCACCTTGCATGTGCCCTGCGATTCGCCTTCGCTG
ATCGAATCCAAGTCGCTCAAGCTGTATCTCAACTCGCTCAACGCCACCCGCTTCAACAGCGCCGAAGCGGTACGCACGCG
CATTGCCACCGACCTGTCCACGCGCGCTGGTGCCGATGTGGCGGTGGAGTTCGGCCTACCGCCGATCGACGCGGTGGGCG
AGGGCGAATCGATCGATGCGCTGGATGTCAGCATCGACGATTACGGCCCGCCGAACGCGGCCTATCTCTGCGCACGCGCG
CAGCCAGCGGTGGAAGAAGTGCTGACCTCGGCCTTGCTCAAATCCAATTGCCCGGTGACTGGTCAGCCGGATTGGGCCAG
CGTCACCTTGCATTACCGCGGCGCACCGATCGATCGCGAGGGATTGCTGCGTTATTTGGTGAGTTTCCGCGACCACGCCG
AGTTTCATGAGCAATGCGTCGAGCGCATTTTCAACGACGTGCTGACCCAGTGCGCGCCGCAGTGGCTGGTGGTGGAAGCG
CGCTACACACGTCGTGGCGGCCTGGACATCAACCCGCTGCGCAGTTCGGCCAGCGTGCCGACGCCGCTGTCGATCTTCCG
CGACCTGCGCCAATAG

Upstream 100 bases:

>100_bases
GGGTGATTGGGCACGAGCGATGCTCTGCGAGTGCTCCATTGGCCCGTGATACGCCTCGCCATACACCTCACGGCCAGCCC
GTGCGGCCTAAAATGGCGGC

Downstream 100 bases:

>100_bases
ATCGCGCTCATGCGGGATCGCGCGGGCTTCACGGCGGCGCGATCCACGCTTAACAGCGCCCCGCGCACCCTGCTGACACC
GCGATGCACAGGGATTGCAG

Product: 7-cyano-7-deazaguanine reductase

Products: NA

Alternate protein names: 7-cyano-7-carbaguanine reductase; NADPH-dependent nitrile oxidoreductase; PreQ(0) reductase [H]

Number of amino acids: Translated: 271; Mature: 271

Protein sequence:

>271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGELPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDVSIDDYGPPNAAYLCARA
QPAVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ

Sequences:

>Translated_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGELPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDVSIDDYGPPNAAYLCARA
QPAVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
>Mature_271_residues
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGELPFIGRDRWHAYELSWLDAQGKPCVATATLHVPCDSPSL
IESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADVAVEFGLPPIDAVGEGESIDALDVSIDDYGPPNAAYLCARA
QPAVEEVLTSALLKSNCPVTGQPDWASVTLHYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ

Specific function: Catalyzes the NADPH-dependent reduction of 7-cyano-7- deazaguanine (preQ0) to 7-aminomethyl-7-deazaguanine (preQ1) [H]

COG id: COG0780

COG function: function code R; Enzyme related to GTP cyclohydrolase I

Gene ontology:

Cell location: Cytoplasm (Probable) [H]

Metaboloic importance: Non_Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the GTP cyclohydrolase I family. QueF type 2 subfamily [H]

Homologues:

Organism=Escherichia coli, GI1789158, Length=270, Percent_Identity=51.4814814814815, Blast_Score=248, Evalue=4e-67,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR016428
- InterPro:   IPR020602 [H]

Pfam domain/function: PF01227 GTP_cyclohydroI [H]

EC number: =1.7.1.13 [H]

Molecular weight: Translated: 29666; Mature: 29666

Theoretical pI: Translated: 4.87; Mature: 4.87

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

2.2 %Cys     (Translated Protein)
0.4 %Met     (Translated Protein)
2.6 %Cys+Met (Translated Protein)
2.2 %Cys     (Mature Protein)
0.4 %Met     (Mature Protein)
2.6 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGELPFIGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECC
QGKPCVATATLHVPCDSPSLIESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADV
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCE
AVEFGLPPIDAVGEGESIDALDVSIDDYGPPNAAYLCARAQPAVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLHYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCC
>Mature Secondary Structure
MNTPEDSTLGREVAYPSGYDPSLLFPIPRAAGRQAIGLTGELPFIGRDRWHAYELSWLDA
CCCCCCCCCCCCCCCCCCCCCCEEEECCCCCCCCEECCCCCCCCCCCCCCEEEEEEEECC
QGKPCVATATLHVPCDSPSLIESKSLKLYLNSLNATRFNSAEAVRTRIATDLSTRAGADV
CCCCEEEEEEEEECCCCCCCCCCCCEEEEEECCCCCCCCCHHHHHHHHHHHHHCCCCCCE
AVEFGLPPIDAVGEGESIDALDVSIDDYGPPNAAYLCARAQPAVEEVLTSALLKSNCPVT
EEEECCCCCCCCCCCCCCEEEEEEECCCCCCCCEEEEECCCHHHHHHHHHHHHHCCCCCC
GQPDWASVTLHYRGAPIDREGLLRYLVSFRDHAEFHEQCVERIFNDVLTQCAPQWLVVEA
CCCCCEEEEEEECCCCCCHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHHCCCEEEEEE
RYTRRGGLDINPLRSSASVPTPLSIFRDLRQ
EECCCCCCCCCCCCCCCCCCCHHHHHHHHCC

PDB accession: NA

Resolution: NA

Structure class: Alpha Beta

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]