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The map label for this gene is def [H]

Identifier: 78049482

GI number: 78049482

Start: 4506857

End: 4507369

Strand: Reverse

Name: def [H]

Synonym: XCV3926

Alternate gene names: 78049482

Gene position: 4507369-4506857 (Counterclockwise)

Preceding gene: 78049492

Following gene: 78049481

Centisome position: 87.04

GC content: 61.99

Gene sequence:

>513_bases
ATGGCTTTGCTCCCAATTCTTGAGTTTCCCGACCCGCGGCTGCGCACCAAGGCGGTGCCGGTCGATGCTGCCGAGGTCAC
CAGCCAGGCGTTCCAGACGCTGCTGGACGACATGTTCCAGACCATGTACGAGGCGCCAGGCATTGGCCTGGCCGCCAGTC
AGGTGGACGTGCACAAGCGATTCATGGTGATCGACATCAGCGACGAGAAAAACCTGCCGCAGGTGTTCGTCAACCCCGAG
ATCGTGAGCAAACAGGGCGAGCAGCTGTATCAGGAAGGCTGCCTGTCGGTGCCCGGCATCTATGCCGATGTCAGCCGCGC
CGACGCGATCACCGTGCGTTATCTCGACCGCCAGGGCCAGGCGCAGGAACTGCAGGCCGACGGGCTGCTGGCCGTGTGCA
TCCAGCACGAGATGGACCACCTGGACGGCAAGCTGTTCGTGGATTATCTCTCGCCGCTCAAGCGCGAAATGGTGCGCAAG
AAACTGGCCAAACAGCGCAAGCATGTGGCTTGA

Upstream 100 bases:

>100_bases
CTGCGCACTATAGTCTAGAAACCGGGGCGTCACGCAAGCATTGCGCTAGAATGACAGCGTCTTGCCGGCGCCGCGCCGGT
GTTTCCAGACGGGTAACCCC

Downstream 100 bases:

>100_bases
GGGCCGGGATTGGGGATTGGGGATTGGCAAAAGCGGGTTTTTGCAGTGTTTTGAATCTGCTGGTGTCGAATCCTCGAATG
CGAAGTCTGCTGCGATCTGG

Product: peptide deformylase

Products: NA

Alternate protein names: PDF 2; Polypeptide deformylase 2 [H]

Number of amino acids: Translated: 170; Mature: 169

Protein sequence:

>170_residues
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPE
IVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELQADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA

Sequences:

>Translated_170_residues
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPE
IVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELQADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRK
KLAKQRKHVA
>Mature_169_residues
ALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFQTMYEAPGIGLAASQVDVHKRFMVIDISDEKNLPQVFVNPEI
VSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQAQELQADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKK
LAKQRKHVA

Specific function: Removes the formyl group from the N-terminal Met of newly synthesized proteins. Requires at least a dipeptide for an efficient rate of reaction. N-terminal L-methionine is a prerequisite for activity but the enzyme has broad specificity at other positions

COG id: COG0242

COG function: function code J; N-formylmethionyl-tRNA deformylase

Gene ontology:

Cell location: Cytoplasm [C]

Metaboloic importance: Essential [C]

Operon status: Not Known

Operon components: None

Similarity: Belongs to the polypeptide deformylase family [H]

Homologues:

Organism=Homo sapiens, GI11641243, Length=162, Percent_Identity=32.7160493827161, Blast_Score=81, Evalue=5e-16,
Organism=Escherichia coli, GI1789682, Length=164, Percent_Identity=54.2682926829268, Blast_Score=182, Evalue=7e-48,
Organism=Drosophila melanogaster, GI24645728, Length=158, Percent_Identity=31.6455696202532, Blast_Score=82, Evalue=2e-16,
Organism=Drosophila melanogaster, GI24645726, Length=157, Percent_Identity=30.5732484076433, Blast_Score=80, Evalue=7e-16,

Paralogues:

None

Copy number: NA

Swissprot (AC and ID): NA

Other databases:

- InterPro:   IPR000181 [H]

Pfam domain/function: PF01327 Pep_deformylase [H]

EC number: =3.5.1.88 [H]

Molecular weight: Translated: 19095; Mature: 18964

Theoretical pI: Translated: 5.27; Mature: 5.27

Prosite motif: NA

Important sites: NA

Signals:

None

Transmembrane regions:

None

Cys/Met content:

1.2 %Cys     (Translated Protein)
3.5 %Met     (Translated Protein)
4.7 %Cys+Met (Translated Protein)
1.2 %Cys     (Mature Protein)
3.0 %Met     (Mature Protein)
4.1 %Cys+Met (Mature Protein)

Secondary structure:

>Translated Secondary Structure
MALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFQTMYEAPGIGLAASQVDVHKR
CCCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FMVIDISDEKNLPQVFVNPEIVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCC
AQELQADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
HHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC
>Mature Secondary Structure 
ALLPILEFPDPRLRTKAVPVDAAEVTSQAFQTLLDDMFQTMYEAPGIGLAASQVDVHKR
CCCCCCCCCCCCCCCCCCCCCHHHHHHHHHHHHHHHHHHHHHHCCCCCCCHHHHHHHHE
FMVIDISDEKNLPQVFVNPEIVSKQGEQLYQEGCLSVPGIYADVSRADAITVRYLDRQGQ
EEEEEECCCCCCCEEEECHHHHHHHHHHHHHHCCCCCCCHHHHCCCCCEEEEEEECCCCC
AQELQADGLLAVCIQHEMDHLDGKLFVDYLSPLKREMVRKKLAKQRKHVA
HHHHHHCCHHHHHHHHHHHHCCHHHHHHHHHHHHHHHHHHHHHHHHHCCC

PDB accession: NA

Resolution: NA

Structure class: Unstructured

Cofactors: NA

Metal ions: NA

Kcat value (1/min): NA

Specific activity: NA

Km value (mM): NA

Substrates: NA

Specific reaction: NA

General reaction: NA

Inhibitor: NA

Structure determination priority: 10.0

TargetDB status: NA

Availability: NA

References: 12024217 [H]